scholarly journals Characterization of Differentially Expressed Genes under Salt Stress in Olive

2021 ◽  
Vol 23 (1) ◽  
pp. 154
Author(s):  
Soraya Mousavi ◽  
Roberto Mariotti ◽  
Maria Cristina Valeri ◽  
Luca Regni ◽  
Emanuele Lilli ◽  
...  
Keyword(s):  
Salt Stress ◽  
Mediterranean Area ◽  
Tolerance Mechanism ◽  
Groundwater Salinization ◽  
Salt Stress Response ◽  
Olive Cultivars ◽  
Moderate Salinity ◽  
High Salt Stress ◽  
Complete Genomic

Climate change, currently taking place worldwide and also in the Mediterranean area, is leading to a reduction in water availability and to groundwater salinization. Olive represents one of the most efficient tree crops to face these scenarios, thanks to its natural ability to tolerate moderate salinity and drought. In the present work, four olive cultivars (Koroneiki, Picual, Royal de Cazorla and Fadak86) were exposed to high salt stress conditions (200 mM of NaCl) in greenhouse, in order to evaluate their tolerance level and to identify key genes involved in salt stress response. Molecular and physiological parameters, as well as plant growth and leaves’ ions Na+ and K+ content were measured. Results of the physiological measurements showed Royal de Cazorla as the most tolerant cultivar, and Fadak86 and Picual as the most susceptible ones. Ten candidate genes were analyzed and their complete genomic, CDS and protein sequences were identified. The expression analysis of their transcripts through reverse transcriptase quantitative PCR (RT-qPCR) demonstrated that only OeNHX7, OeP5CS, OeRD19A and OePetD were upregulated in tolerant cultivars, thus suggesting their key role in the activation of a salt tolerance mechanism.

scholarly journals Molecular Characterization of NDL1-AGB1 Mediated Salt Stress Signaling: Further Exploration of the Role of NDL1 Interacting Partners

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2261
Author(s):  
Nidhi Gupta ◽  
Abhishek Kanojia ◽  
Arpana Katiyar ◽  
Yashwanti Mudgil
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
G Protein ◽  
Salinity Stress ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Negative Regulator ◽  
Salt Stress Response

Salt stress is considered to be the most severe abiotic stress. High soil salinity leads to osmotic and ionic toxicity, resulting in reduced plant growth and crop production. The role of G-proteins during salt stresses is well established. AGB1, a G-protein subunit, not only plays an important role during regulation of Na+ fluxes in roots, but is also involved in the translocation of Na+ from roots to shoots. N-Myc Downregulated like 1 (NDL1) is an interacting partner of G protein βγ subunits and C-4 domain of RGS1 in Arabidopsis. Our recent in-planta expression analysis of NDL1 reported changes in patterns during salt stress. Based on these expression profiles, we have carried out functional characterization of the AGB1-NDL1 module during salinity stress. Using various available mutant and overexpression lines of NDL1 and AGB1, we found that NDL1 acts as a negative regulator during salt stress response at the seedling stage, an opposite response to that of AGB1. On the other hand, during the germination phase of the plant, this role is reversed, indicating developmental and tissue specific regulation. To elucidate the mechanism of the AGB1-NDL1 module, we investigated the possible role of the three NDL1 stress specific interactors, namely ANNAT1, SLT1, and IDH-V, using yeast as a model. The present study revealed that NDL1 acts as a modulator of salt stress response, wherein it can have both positive as well as negative functions during salinity stress. Our findings suggest that the NDL1 mediated stress response depends on its developmental stage-specific expression patterns as well as the differential presence and interaction of the stress-specific interactors.

scholarly journals RsSOS1 Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na+ Homeostasis in Radish (Raphanus sativus L.)

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 458
Author(s):  
Wanting Zhang ◽  
Jingxue Li ◽  
Junhui Dong ◽  
Yan Wang ◽  
Liang Xu ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Functional Characterization ◽  
Vital Role ◽  
Salt Stress Response ◽  
Reading Frame ◽  
Yield And Quality

Radish is a kind of moderately salt-sensitive vegetable. Salt stress seriously decreases the yield and quality of radish. The plasma membrane Na+/H+ antiporter protein Salt Overly Sensitive 1 (SOS1) plays a crucial role in protecting plant cells against salt stress, but the biological function of the RsSOS1 gene in radish remains to be elucidated. In this study, the RsSOS1 gene was isolated from radish genotype ‘NAU-TR17’, and contains an open reading frame of 3414 bp encoding 1137 amino acids. Phylogenetic analysis showed that RsSOS1 had a high homology with BnSOS1, and clustered together with Arabidopsis plasma membrane Na+/H+ antiporter (AtNHX7). The result of subcellular localization indicated that the RsSOS1 was localized in the plasma membrane. Furthermore, RsSOS1 was strongly induced in roots of radish under 150 mmol/L NaCl treatment, and its expression level in salt-tolerant genotypes was significantly higher than that in salt-sensitive ones. In addition, overexpression of RsSOS1 in Arabidopsis could significantly improve the salt tolerance of transgenic plants. Meanwhile, the transformation of RsSOS1△999 could rescue Na+ efflux function of AXT3 yeast. In summary, the plasma membrane Na+/H+ antiporter RsSOS1 plays a vital role in regulating salt-tolerance of radish by controlling Na+ homeostasis. These results provided useful information for further functional characterization of RsSOS1 and facilitate clarifying the molecular mechanism underlying salt stress response in radish.

Characterization of the Polyamine Biosynthetic Pathways and Salt Stress Response in Brachypodium distachyon

2017 ◽  
Vol 37 (2) ◽  
pp. 625-634 ◽  
Author(s):  
Yoshihiro Takahashi ◽  
Misako Tahara ◽  
Yuki Yamada ◽  
Yuka Mitsudomi ◽  
Kaoruko Koga
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Brachypodium Distachyon ◽  
Biosynthetic Pathways ◽  
Salt Stress Response

scholarly journals Characterization of Olive (Olea Europaea L.) Genetic Resources via PCR-Based Molecular Marker Systems

2021 ◽  
Vol 2 (1) ◽  
pp. 26-33
Author(s):  
S. Galatali ◽  
N. Abdul Ghafoor ◽  
E. Kaya
Keyword(s):  
Molecular Marker ◽  
Genetic Resources ◽  
Olea Europaea ◽  
Oil Quality ◽  
Mediterranean Area ◽  
Olea Europaea L ◽  
Olive Cultivars ◽  
Olive Varieties ◽  
Olea Europaéa

Olea europaea L., which is one of the ancient culture species cultivated in the Mediterranean area, has approximately 1200 cultivars. Its wild thype forms from O. europaea subsp. europaea var. sylvestris and its culture type forms from O. europaea subsp. europaea var. europaea. Olive cultivation is multiplied by grafting or cutting whereas wild type olive seedlings derived from seeds of O. europaea L. var. sylvestris. Because they have very big level heterozygosities, the genetic diversity of olive cultivars is quite high that they are predominantly allogamus. This genetic variability causes many confusions for identification of olive cultivars and since both oil quality and olive productivity are traits inherited to a variety, it is urgently needed to solve characterization and evaluation of olive genetic resources. The molecular marker systems are independent from environmental factors and effective technology to both detect homonymous and synonymous of cultivars and identify olive varieties. The current review is aimed to present molecular marker systems for identification and characterization of olive cultivars and emphasize their application for conservation of olive germplasm.

Molecular characterization of a novel soybean gene encoding a neutral PR-5 protein induced by high-salt stress

2009 ◽  
Vol 47 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Hiroyuki Tachi ◽  
Kumiko Fukuda-Yamada ◽  
Toshio Kojima ◽  
Masakazu Shiraiwa ◽  
Hidenari Takahara
Keyword(s):  
Salt Stress ◽  
Molecular Characterization ◽  
High Salt ◽  
Gene Encoding ◽  
Soybean Gene ◽  
High Salt Stress
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