Identification and Expression Profile of Chemosensory Genes in the Small Hive Beetle Aethina tumida

Aethina tumida is a parasite and predator of honeybee causing severe loss to the bee industry. No effective and environmentally friendly methods are available to control this pest at present. Chemosensory genes play key roles in insect behavior which can potentially be used as targets for developing environmentally friendly pest control agents. In this study, the putative chemosensory genes in antennae and forelegs of A. tumida involved in olfaction or contact chemical communication of adults were investigated using RNA transcriptome sequencing and PCR methods. Based on transcriptomic data, unigenes encoding 38 odorant receptors (ORs), 24 ionotropic receptors (IRs), 14 gustatory receptors (GRs), 3 sensory neuron membrane proteins (SNMPs), 29 odorant binding proteins (OBPs), and 22 chemosensory proteins (CSPs) were identified. The analyses of tissue expression profiles revealed that genes encoding 38 ORs, 13 antennal IRs, 11 GRs, 1 SNMP, 24 OBPs, and 12 CSPs were predominately expressed in antennae. No significant differences in expression levels of these genes were found between males and females. Genes encoding 5 non-NMDA iGluRs, 3 GRs, 2 SNMPs, 5 OBPs, and 12 CSPs were predominately expressed in forelegs. RT-PCR assays for SNMPs, OBPs and CSPs further revealed that 3 OBPs (AtumOBP3, 26 and 28) and 3 CSPs (AtumCSP7, 8 and 21) were highly expressed in antennae. Our results enrich the gene inventory of A. tumida and facilitate the discovery of potential novel targets for developing new pest control measures.

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Chemosensory Gene Families in the Oligophagous Pear Pest Cacopsylla chinensis (Hemiptera: Psyllidae)

Insects ◽

10.3390/insects10060175 ◽

2019 ◽

Vol 10 (6) ◽

pp. 175 ◽

Cited By ~ 6

Author(s):

Ji-Wei Xu ◽

Xiu-Yun Zhu ◽

Qiu-Jie Chao ◽

Yong-Jie Zhang ◽

Yu-Xia Yang ◽

...

Keyword(s):

Insect Behavior ◽

Gene Families ◽

Tissue Expression ◽

Specific Expression ◽

Female Head ◽

Chemosensory Gene ◽

Chemosensory Genes ◽

Male Head ◽

Chemosensory Systems ◽

Odorant Binding

Chemosensory systems play an important role in insect behavior, and some key associated genes have potential as novel targets for pest control. Cacopsylla chinensis is an oligophagous pest and has become one of the main pests of pear trees, but little is known about the molecular-level means by which it locates its hosts. In this study, we assembled the head transcriptome of C. chinensis using Illumina sequencing, and 63,052 Unigenes were identified. A total of 36 candidate chemosensory genes were identified, including five different families: 12 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), 7 odorant receptors (ORs), 4 ionotropic receptors (IRs), and 2 gustatory receptors (GRs). The number of chemosensory gene families is consistent with that found in other Hemipteran species, indicating that our approach successfully obtained the chemosensory genes of C. chinensis. The tissue expression of all genes using quantitative real-time PCR (qRT-PCR) found that some genes displayed male head, female head, or nymph-biased specific/expression. Our results enrich the gene inventory of C. chinensis and provide valuable resources for the analysis of the functions of some key genes. This will help in developing molecular targets for disrupting feeding behavior in C. chinensis.

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Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes inAthetis lepigone(Lepidoptera: Noctuidae)

PeerJ ◽

10.7717/peerj.3157 ◽

2017 ◽

Vol 5 ◽

pp. e3157 ◽

Cited By ~ 10

Author(s):

Ya-Nan Zhang ◽

Xiu-Yun Zhu ◽

Ji-Fang Ma ◽

Zhi-Ping Dong ◽

Ji-Wei Xu ◽

...

Keyword(s):

Expression Patterns ◽

Environmentally Friendly ◽

Northern China ◽

Tissue Expression ◽

Odorant Binding Proteins ◽

Chemosensory Protein ◽

Genes Encoding ◽

Odorant Binding ◽

Lepidoptera Noctuidae ◽

Oviposition Sites

The olfaction system of insects plays an important role in mediating various physiological behaviors, including locating hosts, avoiding predators, and recognizing mates and oviposition sites. Therefore, some key genes in the system present valuable opportunities as targets for developing novel green pesticides.Athetis lepigone, a noctuid moth can feed on more than 30 different host plants making it a serious polyphagous pest worldwide, and it has become one of the major maize pests in northern China since 2011. However, there are no reports on effective and environmentally friendly pesticides for the control of this pest. In this study, we identified 28 genes encoding putative odorant binding proteins (OBPs) and 20 chemosensory protein (CSPs) genes based on our previousA. lepigonetranscriptomic data. A tissue expression investigation and phylogenetic analysis were conducted in an effort to postulate the functions of these genes. Our results show that nearly half (46.4%) of theAlOBPsexhibited antennae-biased expression while many of theAlCSPswere highly abundant in non-antennal tissues. These results will aid in exploring the chemosensory mechanisms ofA. lepigoneand developing environmentally friendly pesticides against this pest in the future.

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Transcriptome based identification and tissue expression profiles of chemosensory genes in Blattella germanica (Blattaria: Blattidae)

Comparative Biochemistry and Physiology Part D Genomics and Proteomics ◽

10.1016/j.cbd.2016.03.002 ◽

2016 ◽

Vol 18 ◽

pp. 30-43 ◽

Cited By ~ 9

Author(s):

Dong-Juan Niu ◽

Yan Liu ◽

Xiao-Tong Dong ◽

Shuang-Lin Dong

Keyword(s):

Expression Profiles ◽

Blattella Germanica ◽

Tissue Expression ◽

Chemosensory Genes

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Identification of Chemosensory Genes Based on the Antennal Transcriptomic Analysis of Plagiodera versicolora

Insects ◽

10.3390/insects13010036 ◽

2021 ◽

Vol 13 (1) ◽

pp. 36

Author(s):

Xiaolong Liu ◽

Na Tong ◽

Zheran Wu ◽

Yang Li ◽

Meiqi Ma ◽

...

Keyword(s):

Expression Profiles ◽

Gene Families ◽

Tissue Expression ◽

Vital Role ◽

Plagiodera Versicolora ◽

Male And Female ◽

Functional Studies ◽

Chemosensory Genes ◽

Almost All ◽

Chemosensory Systems

Insects can sense surrounding chemical signals by their accurate chemosensory systems. This system plays a vital role in the life history of insects. Several gene families participate in chemosensory processes, including odorant receptors (ORs), ionotropic receptors (IRs), gustatory receptors (GRs), chemosensory proteins (CSPs), odorant binding proteins (OBPs), and sensory neuron membrane proteins (SNMPs). Plagiodera versicolora (Coleoptera: Chrysomelidae), is a leaf-eating forest pest found in salicaceous trees worldwide. In this study, a transcriptome analysis of male and female adult antennae in P. versicolora individuals was conducted, which identified a total of 98 candidate chemosensory genes including 40 ORs, 7 IRs, 13 GRs, 10 CSPs, 24 OBPs, and 4 SNMPs. Subsequently, the tissue expression profiles of 15 P. versicolora OBPs (PverOBPs) and 39 ORs (PverORs) were conducted by quantitative real-time PCR. The data showed that almost all PverOBPs and PverORs were highly expressed in the male and female antennae. In addition, several OBPs and ORs (PverOBP10, PverOBP12, PverOBP18, PverOR24, and PverOR35) had higher expression levels in female antennae than those in the male antennae, indicating that these genes may be taking part in some female-specific behaviors, such as find mates, oviposition site, etc. This study deeply promotes further understanding of the chemosensory system and functional studies of the chemoreception genes in P. versicolora.

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Identification of chemosensory genes by antennal transcriptome analysis and expression profiles of odorant-binding proteins in parasitoid wasp Aulacocentrum confusum

Comparative Biochemistry and Physiology Part D Genomics and Proteomics ◽

10.1016/j.cbd.2021.100881 ◽

2021 ◽

pp. 100881

Author(s):

Yi-jiangcheng Li ◽

Hong-chao Chen ◽

Tian-le Hong ◽

Meng-wen Yan ◽

Jiao Wang ◽

...

Keyword(s):

Transcriptome Analysis ◽

Binding Proteins ◽

Expression Profiles ◽

Parasitoid Wasp ◽

Odorant Binding Proteins ◽

Chemosensory Genes ◽

Odorant Binding

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IPM—A Problem-based Learning Approach

HortScience ◽

10.21273/hortsci.33.3.509e ◽

1998 ◽

Vol 33 (3) ◽

pp. 509e-509

Author(s):

Robert P. Rice

Keyword(s):

Pest Control ◽

Test Performance ◽

Management Strategies ◽

Ornamental Plants ◽

Real Life ◽

Problem Based Learning ◽

Control Measures ◽

Learning Approach ◽

Pest Monitoring ◽

Use Of Resources

The Cal Poly philosophy of “Learn and Understand by Doing” has been integrated with problem-based learning and the use of the latest technology to produce a class that closely simulates real-life pest control situations. Goals of the class, Disease and Pest Control Systems for Ornamental Plants, are to teach students pest monitoring, control and problem solving techniques, the use of resources including the internet and journals, and the use of the latest pest control equipment and application techniques. Students are shown pest situations and then work in groups to diagnose the problem, investigate management strategies, apply control measures, and monitor results. Weekly class presentations inform the class of the various projects and help to teach the class organization and presentation skills. Student evaluations and test performance have demonstrated that students achieve class objectives substantially better with the problem-based learning approach than with the previous lecture-based approach to the class.

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Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer

Biology ◽

10.3390/biology10060500 ◽

2021 ◽

Vol 10 (6) ◽

pp. 500

Author(s):

Jeeyong Lee ◽

Junhye Kwon ◽

DaYeon Kim ◽

Misun Park ◽

KwangSeok Kim ◽

...

Keyword(s):

Candidate Genes ◽

Expression Profiles ◽

Cell Cycle Control ◽

Methylation Status ◽

Locally Advanced ◽

Gene Expression Profiles ◽

Advanced Rectal Cancer ◽

Bioinformatic Analysis ◽

Locally Advanced Rectal Cancer ◽

Genes Encoding

LARC patients were sorted according to their radio-responsiveness and patient-derived organoids were established from the respective cancer tissues. Expression profiles for each group were obtained using RNA-seq. Biological and bioinformatic analysis approaches were used in deciphering genes and pathways that participate in the radio-resistance of LARC. Thirty candidate genes encoding proteins involved in radio-responsiveness–related pathways, including the immune system, DNA repair and cell-cycle control, were identified. Interestingly, one of the candidate genes, cathepsin E (CTSE), exhibited differential methylation at the promoter region that was inversely correlated with the radio-resistance of patient-derived organoids, suggesting that methylation status could contribute to radio-responsiveness. On the basis of these results, we plan to pursue development of a gene chip for diagnosing the radio-responsiveness of LARC patients, with the hope that our efforts will ultimately improve the prognosis of LARC patients.

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