scholarly journals Temporal Trend of Conventional Sperm Parameters in a Sicilian Population in the Decade 2011–2020

2021 ◽  
Vol 10 (5) ◽  
pp. 993
Author(s):  
Rossella Cannarella ◽  
Rosita A. Condorelli ◽  
Carmelo Gusmano ◽  
Nunziata Barone ◽  
Nunziatina Burrello ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Pooled Data ◽  
Dependent Change ◽  
Time Dependent Change ◽  
The City ◽  
Over Time

Objective: To evaluate the changes of conventional sperm parameters in men who referred to an andrology reference center in Catania (Eastern Sicily, Italy) in the decade 2011–2020. Methods: For this purpose, we selected–retrospectively and randomly–the reports of 1409 semen analyses performed according to the 2010 WHO criteria. Data on sperm concentration, total sperm count, progressive sperm motility, and percentage of normal forms were analyzed using linear regression of the raw and logarithmic-transformed data. The sperm parameters were subsequently pooled in two five-year periods (2011–2015 and 2016–2020) and compared with each other. Finally, the influence of the city of residence was assessed on five-year pooled data. Main results: A slight but non-significant decline of total sperm count (−2.26 million/year; p = 0.065) and the percentage of spermatozoa with normal morphology (−0.08%/year; p = 0.057) was observed. In contrast, a significant increase of progressive sperm motility (+0.28%/year; p = 0.008) over time was found. The total sperm count of the quinquennium 2016–2020 was significantly lower. and an upward trend of progressive sperm motility was found. compared to the years 2011–2015. No changes in sperm concentration and morphology occurred in the years 2011–2015 vs. 2016–2020. Sperm conventional parameters did not differ when the five-year pooled data were analyzed according to the town of residence. Conclusions: Divergent trends of total sperm count and progressive sperm motility over time were found in patients from Eastern Sicily. This may point out the need of assessing whether a time-dependent change of biofunctional sperm parameters occurs to really understand the trend of sperm quality over time.

scholarly journals From Spermiogram to Bio-Functional Sperm Parameters: When and Why Request Them?

2020 ◽  
Vol 9 (2) ◽  
pp. 406 ◽  
Author(s):  
Rosita A. Condorelli ◽  
Aldo E. Calogero ◽  
Giorgio I. Russo ◽  
Sandro La Vignera
Keyword(s):  
Normal Form ◽  
Sperm Motility ◽  
Sperm Count ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Sperm Parameter ◽  
Progressive Motility ◽  
Semen Volume ◽  
Chromatin Compactness

The aim of this experimental study was to evaluate whether infertile patients may benefit from the evaluation of bio-functional sperm parameters in addition to the conventional semen analysis. To accomplish this, we evaluated the correlation between conventional and bio-functional sperm parameters based on their percentile distribution in search of a potential threshold of these latter that associates with conventional sperm parameter abnormalities. The study was conducted on 577 unselected patients with infertility lasting at least 12 months. We identified cut-off values according to the median of the population for mitochondrial membrane potential (MMP), number of alive spermatozoa, and chromatin abnormality. High MMP (HMMP) (≥46.25%) was associated with sperm concentration, sperm count, progressive motility, and normal form. Low MMP (LMMP) (≥36.5%) was found to be associated with semen volume, sperm concentration, total sperm count, progressive motility, total motility, and normal form. The number of alive spermatozoa (≥71.7%) was associated with sperm concentration and progressive motility whereas abnormal chromatin compactness (≥21.10%) was associated with sperm concentration, total sperm count, and progressive motility. The data would suggest that, for every increase in the percentile category of sperm concentration, the risk of finding an HMMP≤46.25 is reduced by 0.4 and by 0.66 for a total sperm count. This risk is also reduced by 0.60 for every increase in the percentile category of sperm progressive motility and by 0.71 for total sperm motility. Each increment of percentile category of the following sperm parameter was followed by a decrease in the risk of finding an LMMP≤36.5: sperm concentration 1.66, total sperm count 1.28, sperm progressive motility 1.27, total sperm motility 1.76, and normal form 1.73. Lastly, the data showed that, for every increase in the percentile category of total sperm count, the risk of finding an abnormal chromatin compactness ≤21.10 is reduced by 1.25 (1.04–1.51, p < 0.05) and an increase of total sperm motility is associated with a reduced risk by 1.44 (1.12–1.85, p < 0.05). Results suggest a correlation between bio-functional and conventional sperm parameters that impact the sperm fertilizing potential. Therefore, the evaluation of bio-functional sperm parameters by flow cytometry may be useful to explain some cases of idiopathic male infertility.

Dietary Supplementation of Antioxidants Improves Semen Quality of IVF Patients in Terms of Motility, Sperm Count, and Nuclear Vacuolization

2012 ◽  
Vol 82 (6) ◽  
pp. 391-398 ◽  
Author(s):  
Barbara Wirleitner ◽  
Pierre Vanderzwalmen ◽  
Astrid Stecher ◽  
Dietmar Spitzer ◽  
Maximilian Schuff ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Morphological Changes ◽  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Sperm Parameters ◽  
Class I ◽  
Antioxidative Therapy

Background: This study aimed to investigate the influence of an oral antioxidative supplementation on sperm quality of in vitro fertilization (IVF) patients, as analyzed by sperm motility according to the WHO criteria and motile sperm organelle morphology examination (MSOME). Methods: Semen samples were collected from 147 patients before undergoing an IVF/intracytoplasmic morphologically-selected sperm injection (IMSI) cycle and 2 - 12 months after an antioxidative supplementation. Semen analysis was evaluated according to WHO and MSOME criteria. Spermatozoa were grouped according to the size of nuclear vacuoles within the sperm’s heads. Patients were divided into oligoasthenoteratozoospermic (OAT) and non-OAT men. Between first and second semen analysis, patients were supplemented orally with an antioxidative preparation. Results: After the antioxidative therapy we observed a significant reduction in the percentage of immotile sperm cells in the patients. Additionally, the percentage of class I spermatozoa according to MSOME criteria was significantly higher after antioxidative supplementation. In OAT patients the percentage of class I sperm was found to be increased, although not significantly. However, we observed a drastic improvement in sperm motility as well as in total sperm count in this group. Conclusion: The results demonstrated a considerable improvement in semen quality, notably in OAT patients. Considering the putative relationship between semen quality on the one hand and reactive oxygen species on the other, the observed changes in the sperm parameters indicate that a decline in semen quality, and even subtle morphological changes, might be associated with oxidative stress. Our findings suggest that an antioxidative and micronutrient supplementation has a remarkable benefit for IVF patients having restricted sperm parameters, in particular.

scholarly journals Effect of hydroxyurea exposure before puberty on sperm parameters in males with sickle cell disease

Blood ◽  
2020 ◽  
Author(s):  
Laure Joseph ◽  
Camille Jean ◽  
Sandra Manceau ◽  
Celine Chalas ◽  
Cecile Arnaud ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Specific Effect ◽  
Sperm Parameters ◽  
Cell Disease ◽  
Transfusion Therapy ◽  
Young Infants

Sperm parameters are known to be impaired in men with sickle cell disease (SCD). Although treatment with hydroxyurea (HU) impacts sperm quality, sperm preservation is impossible before puberty. The present study's primary objective was to analyze and compare sperm parameters in male SCD patients exposed (or not) to HU before puberty. Twenty-six sperm samples from 15 patients (median (range) age: 17 (16-23)) treated with HU during childhood were compared with 46 samples from 23 HU-naïve patients (median (range) age: 20 (16-24)). The median (range) age at HU initiation was 6 years (1-14), the median duration of HU treatment was 4 years (0.5-10) and the mean ± standard deviation dose of HU was 22.4 ± 3.7 mg/kg/day. Although we observed substantial quantitative and qualitative semen abnormalities in all patients, there were no significant differences in semen volume, sperm concentration, total sperm count, and spermatozoa motility, morphology and vitality between the HU-exposed and HU-naïve groups. At the time of the semen analysis, respectively 100% and 52% of the patients in the HU-exposed and HU-naïve groups were on transfusion therapy. Hydroxyurea's specific effect on spermatogenesis in very young infants and the putative value of transfusion for reversing HU's toxicity warrant further investigation.

Determinants of infertility in couples and methods for their correction

2021 ◽  
Vol 20 (1) ◽  
pp. 105-112
Author(s):  
E.V. Shikh ◽  
◽  
A.A. Makhova ◽  
E.D. Khaytovich ◽  
◽  
...  
Keyword(s):  
Sperm Motility ◽  
Male Fertility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Added Sugar ◽  
Trans Fats ◽  
Factors Affecting ◽  
Micronutrient Intake ◽  
Positive Effect

Nutrition is one of the main environmental factors affecting the reproductive system. The modern diet is characterized by higher consumption of high-calorie foods, saturated fats, trans fats, refined grains and added sugar. Despite the heterogeneity of the populations studied, in general, the data confirm a steady downward trend in male sperm quality in most Western countries, including an increase in the prevalence of conditions such as complete asthenozoospermia (lack of motility) and isolated asthenozoospermia (low sperm motility due to varicocele, infections or genetic factors). Clear patterns between nutrition and fertility have been identified. Studies have shown a significant positive effect of additional exogenous micronutrient intake on total sperm count (ω-3 and CoQ10); sperm concentration (selenium, zinc, ω-3 and CoQ10); sperm motility (selenium, zinc, ω-3, CoQ10 and carnitine); morphology (selenium, ω-3, CoQ10 and carnitine). Additional exogenous intake of micronutrients that affect sperm can be used as an effective and safe factor in modulating male fertility. Key words: coenzyme Q10, lipoic acid, male fertility, selenium, sperm, zinc

scholarly journals Improvement in Sperm Parameters With Traditional Iranian Remedy

2016 ◽  
Vol 22 (2) ◽  
pp. 223-226 ◽  
Author(s):  
Farnaz Sohrabvand ◽  
Somaye Mahroozade ◽  
Sodabe Bioos ◽  
Seyed Mohammad Nazari ◽  
Fataneh Hashem Dabaghian
Keyword(s):  
Male Infertility ◽  
Traditional Medicine ◽  
Assisted Reproductive Technology ◽  
Intracytoplasmic Sperm Injection ◽  
Sperm Quality ◽  
Intrauterine Insemination ◽  
Sperm Count ◽  
Reproductive Technology ◽  
Sperm Parameters ◽  
Medicinal Treatment

Introduction. Idiopathic male infertility is a global problem with almost no definite medicinal treatment. Most patients have to go through intrauterine insemination or assisted reproductive technology for achieving fertility. Unfortunately, success rates are low in cases with very low sperm count. Therefore it seems that improvement in sperm quality can have beneficial effects on assisted reproductive technology outcome. Case Report. A 39-year-old man with history of infertility for 6 years was referred to the traditional medicine clinic with a recurrent unsuccessful intracytoplasmic sperm injection trial. His sperm analysis showed severe oligoasthenoteratozoospermia. After taking a traditional remedy he had a remarkable improvement in his sperm parameters, which led to the formation of 8 embryos in the following intracytoplasmic sperm injection cycle. Conclusion. Traditional medicine presents various food and remedy options for treating male infertility. It seems that combination therapy can be beneficial in obtaining better results in treatment of male idiopathic infertility.

scholarly journals Semen quality and diversity of morphological sperm abnormalities in bulls: breed and strain effects

2019 ◽  
Vol 22 (8) ◽  
pp. 931-938
Author(s):  
M. A. Kleshchev ◽  
V. L. Petukhov ◽  
L. V. Osadchuk
Keyword(s):  
Sperm Motility ◽  
Environmental Conditions ◽  
Western Siberia ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Sperm Head ◽  
Sperm Dna Fragmentation ◽  
Sperm Production ◽  
Sperm Abnormalities

At present great attention is paid to studying genetic regulation of farm animal adaptations to environmental conditions. This problem is very important due to a wide expansion of highly productive cattle breeds created in Europe and North America. However, until the present no investigation of changing semen quality in bulls of imported breeds during their adaptations to environmental conditions of Western Siberia has been conducted. The aim of this study was to investigate semen quality peculiarities and the diversity of morphological sperm abnormalities in bulls of imported and local breeds kept in the environmental conditions of the southern part of Western Siberia. We determined sperm concentration, sperm count, and rate of sperm with progressive motility and percentage of morphologically normal spermatozoa. The rate of sperm abnormalities according to Blome’s classifcation was determined too. It was found that the mean values of sperm concentration, sperm motility and percentage of morphologically normal spermatozoa in the bulls investigated were similar to those in bulls kept in European countries. Inter­breed differences in these parameters were not found. However, bulls of the Red Danish, Angler, and Simmental breeds had a higher percentage of misshapen sperm head and pyriform sperm head than bulls of the Black­White breed. An inter­strain difference in sperm motility in bulls of the Black­White breed was observed. It was found that bulls of Reflection Sovereign 198998 strain had lover sperm motility than bulls of Wis Burke Ideal 1013415 strain. No inter­strain differences in sperm production, percentage of morphologically normal spermatozoa and rate of main sperm abnormalities were found. Thus, it has been found that the environmental conditions of the southern part of Western Siberia do not seriously affect the sperm production, sperm motility or percentage of morphologically normal spermatozoa in bulls. However, the increased rate of misshapen and pyriform sperm heads in the bulls of the foreign breeds points to a need to study sperm DNA fragmentation.

scholarly journals The Association Between Race, Obesity, and Sperm Quality Among Men Attending a University Physician Practice in Washington, DC

2020 ◽  
Vol 14 (3) ◽  
pp. 155798832092598
Author(s):  
Nathan L. McCray ◽  
Heather A. Young ◽  
Michael S. Irwig ◽  
David Frankfurter ◽  
Arnold M. Schwartz ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
World Health ◽  
Washington Dc ◽  
Physician Practice ◽  
Research Attention ◽  
Men Of Color ◽  
Hispanic Men

A decades-long decline in sperm counts in Western countries has coincided with an increase in obesity rates, prompting study into their association. Few of these studies have incorporated men of color, the sperm health of whom is relatively unknown. The present exploratory study evaluated the association between body mass index (BMI), race, ethnicity, and sperm parameters among a diverse sample of U.S. men attending a Washington, DC physician practice. Semen samples were collected and processed at a single laboratory and sperm concentration, motility, morphology, and count were evaluated according to World Health Organization (WHO) 5th edition criteria. Multivariate models accounted for covariates related to sperm health. The study population ( n = 128) was largely obese (45.3%) or overweight (34.4%), and 36.0% were black or Hispanic. Black men had lower adjusted sperm concentration compared to white men (75.0 million/mL to 107.4 million/mL, p = .01) and were more likely to have oligozoospermia ( p = .01), asthenozoospermia ( p = .004), and low sperm count ( p < .0001). Hispanic men had higher adjusted sperm concentration compared to non-Hispanic men (124.5 million/mL to 62.1 million/mL, p = .007) and were less likely to have teratozoospermia ( p = .001). Obesity and BMI were associated with lower sperm motility and count in crude models only. Given the study’s sample size its findings should be interpreted with caution but align with the limited epidemiological literature to date that has evaluated racial and ethnic differences in semen quality. Heightened clinical research attention is needed to ensure men of color are included in representative numbers in studies of urologic and andrologic health.

294 EFFECT OF SPERMATOZOA MOTILITY ON TRANSFERABLE EMBRYO RATE OF SUPEROVULATED JAPANESE BLACK CATTLE

2009 ◽  
Vol 21 (1) ◽  
pp. 244
Author(s):  
K. Hayama ◽  
M. Takeuchi ◽  
A. Ideta ◽  
M. Urakawa ◽  
M. Sasatani ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Electronic Systems ◽  
Embryo Production ◽  
Spermatozoa Motility ◽  
Multiple Comparison Test ◽  
Medical Electronic ◽  
D 20

Sperm motility is known to affect fertilization; however, little is known about the relationship between frozen–thawed sperm motility and in vivo fertilization following superovulatory treatment. The objective of this study was to evaluate a sperm function test as potential predictors of embryo production following superovulatory treatment in cattle. Two to five batches of semen (Japanese black bull, n = 4, A to D) were diluted with egg york-citrate-glycerol in 0.5 mL plastic straws, and they were stored in liquid nitrogen until analyzed. Frozen–thawed spermatozoa were evaluated for motility {motile sperm concentration (MSC, million mL–1), progressive MSC (PMSC, million mL–1) and velocity (μm s–1)} using a sperm quality analyzer for bulls (SQA-Vb, Medical Electronic Systems, Caesarea, Israel). Each sample of 20 μL aspirated into the disposable capillary, was inserted into SQA-Vb. Measurements were displayed within 75 s. Intra-assay CVs of MSC, PMSC, and velocity were 14.2, 7.3 and 7.5%, respectively. Inter-assey CVs of them were 13.5, 3.9 and 4.3% respectively. Superstimulated donors (Japanese black cows, n = 161) were artificially inseminated with one dose of frozen–thawed semen (bull A = 74, B = 46, C = 21 and D = 20). The proportion of transferable embryo (IETS grade 1 to 3) was examined on day 7 (day 0 = estrus). Data were analyzed using ANOVA followed by Scheffe multiple comparison test, and Fisher’s z-transformation. MSC, PMSC and velocity values differed significantly among each bull. The values of bull A were much lower than those of the other bulls. The proportion of transferable embryos produced by bull A was significantly lower than that of other bulls (P < 0.05, Table 1). Correlations showed significant association between MSC and proportion of transferable embryos (r = 0.99, P < 0.01). We conclude that bovine sperm motility using a SQA-Vb is a useful predictor of embryo production following superovulatory treatment. Table 1.Relationship between sperm motility and proportion of transferable embryo

23 Sperm quality of Pure Spanish stallions is affected by inbreeding coefficient and age

2020 ◽  
Vol 32 (2) ◽  
pp. 137
Author(s):  
Y. Pirosanto ◽  
M. Valera ◽  
A. Molina ◽  
J. Dorado ◽  
S. Demyda-Peyrás
Keyword(s):  
Sperm Motility ◽  
Inbreeding Depression ◽  
Negative Correlation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Inbreeding Coefficient ◽  
Semen Parameters ◽  
Computer Assisted

Inbreeding depression, a genetic condition produced by the mating of close-related individuals, has been associated with a reduction of fertility in several species. However, a loss in sperm quality was also associated with age. In horses, the few existing reports have described a tendency of both parameters to produce a negative effect on sperm quality. However, those reports were performed using a subjective evaluation of sperm motility. In the present study, a total of 692 ejaculates from 86 Pure Spanish stallions (PRE), aged between 3 and 22 years, were evaluated using a computer-assisted methodology to determine the effect of inbreeding in four semen parameters: free-gel volume (V), sperm concentration (C, by haemocytometer), and total (TM) and progressive (PM) sperm motility (by Spermvision sperm class analyser; Minitube). The inbreeding coefficient (F) was estimated using 300 000 PRE pedigree records approximately (minimum pedigree depth, eight equivalent complete generations; range, between 1 and 30.1%). Stallion, age, ejaculate, and season of semen collection were the variables included in the statistical model (general linear model), with ejaculate and season being the variables with a major effect (by variance components analysis). Our results showed that sperm concentration (r=−0.18; P&lt;0.0001) and volume (to a lesser extent) were reduced with advancing age, both showing a major decline after 15 years of age. To the contrary, sperm motility was not affected by age of the stallion. We also found a negative correlation between the inbreeding coefficient and ejaculate volume (r=−0.14; P&lt;0.001), with a marked decrease seen when F was between 7 and 20%. Also, a negative correlation was observed in PM (r=−0.08; P&lt;0.05), although to a lower extent. Conversely, C and TM were not affected by inbreeding depression (P&gt;0.05). In conclusion, our results demonstrated that high levels of inbreeding can compromise severely the sperm quality of the PRE stallion, which, subsequently, may have a negative influence on fertility. Ongoing studies using genomic data will help to detect genetic variants associated with stallion semen quality and how it is influenced by inbreeding in specific genomic regions.

scholarly journals Improvement of Sperm Quality in Hyperviscous Semen following DNase I Treatment

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Effrosyni Nosi ◽  
Angelos D. Gritzapis ◽  
Konstantinos Makarounis ◽  
Georgios Georgoulias ◽  
Vasilios Kapetanios ◽  
...  
Keyword(s):  
Density Gradient ◽  
Sperm Motility ◽  
Density Gradient Centrifugation ◽  
Sperm Quality ◽  
Sperm Count ◽  
Gradient Centrifugation ◽  
Dnase I ◽  
Control Group ◽  
First Case ◽  
Dnase Treatment

Semen hyperviscosity impairs sperm motility and can lead to male infertility. This prospective study aimed at assessing the ability of exogenous DNase in improving sperm quality, taking into consideration that DNase has been found in the seminal plasma of several species and that neutrophils release chromatin in order to trap bacteria. A total of seventy-seven semen samples with high seminal viscosity (HSV) as the study group and sixty-two semen samples with normal seminal viscosity (NSV) as the control group were compared in this analysis. These semen samples were divided into three groups of receiving treatment (a) with DNase I at 37°C for 15 min, (b) by density gradient centrifugation, and (c) with a combination of the above two methods. Following a fifteen-minute treatment of hyperviscous semen, the motility of spermatozoa in 83% of semen samples increased to a statistically significant degree. On the contrary, DNase treatment of semen with normal viscosity had no such effects. The above treatment was also accompanied by a significant increase in the percentage of normal spermatozoa, resulting in a major decrease of the teratozoospermia index. Comparison between semen samples that underwent density gradient centrifugation following DNase I treatment, to those collected after density gradient treatment alone, showed that in the first case the results were more spectacular. The evaluation of each preparation in terms of yield (% total progressively motile sperm count after treatment in relation to the initial total sperm count) revealed that the combined approach resulted in 29.8% vs. 18.5% with density treatment alone (p=0.0121). DNase I treatment results in an improvement of sperm motility and morphology and could be beneficial to men with hyperviscous semen in assisted reproduction protocols.

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