Bioactive Compounds from Mangrove Endophytic Fungus and Their Uses for Microorganism Control

Mangroves are ecosystems with unique characteristics due to the high salinity and amount of organic matter that house a rich biodiversity. Fungi have aroused much interest as they are an important natural source for the discovery of new bioactive compounds, with potential biotechnological and pharmacological interest. This review aims to highlight endophytic fungi isolated from mangrove plant species and the isolated bioactive compounds and their bioactivity against protozoa, bacteria and pathogenic viruses. Knowledge about this type of ecosystem is of great relevance for its preservation and as a source of new molecules for the control of pathogens that may be of importance for human, animal and environmental health.

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Biological potential and chemical composition of bioactive compounds from endophytic fungi associated with thai mangrove plants

South African Journal of Botany ◽

10.1016/j.sajb.2021.04.031 ◽

2021 ◽

Vol 141 ◽

pp. 66-76

Author(s):

Kathawut Sopalun ◽

Wanpen Laosripaiboon ◽

Anyalak Wachirachaikarn ◽

Siriluck Iamtham

Keyword(s):

Chemical Composition ◽

Bioactive Compounds ◽

Endophytic Fungi ◽

Mangrove Plants ◽

Biological Potential

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Low Temperature Illitization through Illite-Dioctahedral Vermiculite Mixed Layers in a Tropical Saline Lake Rich in Hydrothermal Fluids (Sochagota Lake, Colombia)

Minerals ◽

10.3390/min11050523 ◽

2021 ◽

Vol 11 (5) ◽

pp. 523

Author(s):

Gabriel Ricardo Cifuentes ◽

Juan Jiménez-Millán ◽

Claudia Patricia Quevedo ◽

Fernando Nieto ◽

Javier Cuadros ◽

...

Keyword(s):

Electron Microscopy ◽

Organic Matter ◽

Low Temperature ◽

High Salinity ◽

Source Rocks ◽

Temperate Climate ◽

Lake Basin ◽

X Ray ◽

Saline Waters ◽

Mixed Layers

In this investigation, we showed that high salinity promoted by hydrothermal inputs, reducing conditions of sediments with high content in organic matter, and the occurrence of an appropriate clay mineral precursor provide a suitable framework for low-temperature illitization processes. We studied the sedimentary illitization process that occurs in carbonaceous sediments from a lake with saline waters (Sochagota Lake, Colombia) located at a tropical latitude. Water isotopic composition suggests that high salinity was produced by hydrothermal contribution. Materials accumulated in the Sochagota Lake’s southern entrance are organic matter-poor sediments that contain detrital kaolinite and quartz. On the other hand, materials formed at the central segment and near the lake exit (north portion) are enriched in organic matter and characterized by the crystallization of Fe-sulfides. X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), high resolution transmission electron microscopy (HRTEM), and energy dispersive X-ray spectrometry (EDX) data allowed for the identification of illite and illite-dioctahedral vermiculite mixed layers (I-DV), which are absent in the southern sediments. High humidity and temperate climate caused the formation of small-sized metastable intermediates of I-DV particles by the weathering of the source rocks in the Sochagota Lake Basin. These particles were deposited in the low-energy lake environments (middle and north part). The interaction of these sediments enriched in organic matter with the saline waters of the lake enriched in hydrothermal K caused a reducing environment that favored Fe mobilization processes and its incorporation to I-DV mixed layers that acted as mineral precursor for fast low temperature illitization, revealing that in geothermal areas clays in lakes favor a hydrothermal K uptake.

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Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal

F1000Research ◽

10.12688/f1000research.23250.1 ◽

2020 ◽

Vol 9 ◽

pp. 379 ◽

Cited By ~ 1

Author(s):

Dhurva Prasad Gauchan ◽

Pratistha Kandel ◽

Astha Tuladhar ◽

Ashesh Acharya ◽

Upendra Kadel ◽

...

Keyword(s):

Antimicrobial Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Bioactive Compounds ◽

Endophytic Fungi ◽

Diffusion Method ◽

Dry Extract ◽

Brine Shrimp Lethality Assay ◽

Fungal Extracts ◽

Taxus Wallichiana

Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties. Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts. Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata, Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC50 of 104.2 and 125.9µg/ml, respectively. Conclusions: The crude fungal extracts obtained from endophytes: A. alternata, C. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents.

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