scholarly journals First Experimental Study of the Influence of Extracellular Vesicles Derived from Multipotent Stromal Cells on Osseointegration of Dental Implants

2021 ◽  
Vol 22 (16) ◽  
pp. 8774
Author(s):  
Igor Maiborodin ◽  
Aleksandr Shevela ◽  
Vera Matveeva ◽  
Vitaly Morozov ◽  
Michael Toder ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Dental Implants ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
Soft Tissues ◽  
Control Group ◽  
Complete Fusion ◽  
Multipotent Stromal Cells ◽  
Tibial Condyle ◽  
Histologic Pattern

Herein, the aim was to study the state of the bone tissue adjacent to dental implants after the use of extracellular vesicles derived from multipotent stromal cells (MSC EVs) of bone marrow origin in the experiment. In compliance with the rules of asepsis and antiseptics under general intravenous anesthesia with propofol, the screw dental implants were installed in the proximal condyles of the tibia of outbred rabbits without and with preliminary introduction of 19.2 μg MSC EVs into each bone tissue defect. In 3, 7, and 10 days after the operation, the density of bone tissue adjacent to different parts of the implant using an X-ray unit with densitometer was measured. In addition, the histological examinations of the bone site with the hole from the removed device and the soft tissues from the surface of the proximal tibial condyle in the area of intra-bone implants were made. It was found out that 3 days after implantation with the use of MSC EVs, the bone density was statistically significantly higher by 47.2% than after the same implantation, but without the injection of MSC EVs. It is possible that as a result of the immunomodulatory action of MSC EVs, the activity of inflammation decreases, and, respectively, the degree of vasodilation in bones and leukocyte infiltration of the soft tissues are lower, in comparison with the surgery performed in the control group. The bone fragments formed during implantation are mainly consolidated with each other and with the regenerating bone. Day 10 demonstrated that all animals with the use of MSC EVs had almost complete fusion of the screw device with the bone tissue, whereas after the operation without the application of MSC EVs, the heterogeneous histologic pattern was observed: From almost complete osseointegration of the implant to the absolute absence of contact between the foreign body and the new formed bone. Therefore, the use of MSC EVs during the introduction of dental implants into the proximal condyle of the tibia of rabbits contributes to an increase of the bone tissue density near the device after 3 days and to the achievement of consistently successful osseointegration of implants 10 days after the surgery.

scholarly journals Morphology of the Myocardium after Experimental Bone Tissue Trauma and the Use of Extracellular Vesicles Derived from Mesenchymal Multipotent Stromal Cells

2021 ◽  
Vol 11 (11) ◽  
pp. 1206
Author(s):  
Igor Maiborodin ◽  
Marina Klinnikova ◽  
Sergey Kuzkin ◽  
Vitalina Maiborodina ◽  
Sergey Krasil’nikov ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Vessels ◽  
Bone Tissue ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
The Body ◽  
Simultaneous Increase ◽  
Multipotent Stromal Cells ◽  
Vascular Walls ◽  
Mesenchymal Multipotent Stromal Cells

The effect of extracellular vesicles (EVs) of various origins on the heart structures in the time of health and disease has been well studied. At the same time, data on the distribution of EVs throughout the body after introduction into the tissues and the possibility of the influence of these EVs on organs distant from the injection site are practically absent. It is also necessary to note a certain inconsistency in the results of various researchers: from articles on the direct absorption of EVs derived from mesenchymal multipotent stromal cells (MSC EVs) by cardiomyocytes to the data that the heart is inherently immune to drug delivery mediated by nanoparticles. In this regard, the morphological changes in the myocardium of outbred rabbits of both sexes weighing 3–4 kg were studied at various times after experimental trauma of the bone tissue in the proximal condyle of the tibia (PCT) and the use of MSC EVs. As a result of modeling the PCT defect, rabbits develop myocardial edema in the heart muscle by the 3rd day, their lymphatic vessels expand, and then, on the 7th day, the blood vessels become dilated. In the myocardium, the relative and absolute contents of neutrophils, erythrocytes, and macrophages increase, but the percentage of lymphocytes decreases. By day 10, almost all of these changes return to their initial values. The detected transformations of the myocardium are most likely due to the ingress of detritus with the blood flow from the PCT. The use of MSC EVs to influence the regeneration of damaged tissue of PCT promotes earlier dilatation of the blood vessels of the heart with pronounced diapedesis of erythrocytes or even hemorrhages, prolongation of edema, the formation of blood clots in vessels with obliteration of their lumen, sclerotic transformation of vascular walls and paravascular tissues. In the myocardium, the number density of neutrophils, the percentage of lymphocytes, and neutrophils become smaller, with a simultaneous increase in the relative numbers of erythrocytes and macrophages, and changes in the content of macrophages remained until the end of the observation—up to 10 days after the surgery. The discovered effect of MSC EVs is most likely associated with the suppression of the activity of the inflammatory process in the PCT area, which, in turn, was caused by a longer ingress of detritus with blood flow into the myocardium. The absence of statistically significant differences between changes in the myocardium of the left and right ventricles may indicate that both detritus from the surgical site and MSC EVs affect the heart spreading through the coronary artery system.

scholarly journals THE STUDY OF STRUCTURAL CHANGES IN BONE TISSUE OF ALVEOLAR PROCESS OF JAWS IN EXPERIMENTAL ANIMALS AFTER IMPLANTATION OF A NEW CONSTRUCTION OF DENTAL IMPLANT FROM TITANIUM BT-1.00 DEVELOPED IN UZBEKISTAN

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Nigman Lukmanovich Khabilov ◽  
Tatyana Olegovna Mun ◽  
Farkhodjon Komiljonovich Usmonov ◽  
Iskander Mukhamedovich Baybekov
Keyword(s):  
Bone Tissue ◽  
Dental Implants ◽  
Dental Implant ◽  
Structural Changes ◽  
Soft Tissues ◽  
Control Group ◽  
Dental Implantation ◽  
Group 12 ◽  
New Construction ◽  
Implant System

The widespread introduction of dental implant in the dental practice will determine progress and the future of prosthetic dentistry. At the same time, like any new direction dental implantation has generated a lot of questions and unsolved problems. On many of these issues and problems can be answered only by using morphological methods. Therefore, after the development of a new construction of the dental implant one of the main issues was the question of its interaction with the jawbone and soft tissues of the oral cavity.We performed the experiment on 24 long snout dogs of both sexes, weighing 30-35 kg, with normal bite, at the age of 20-24 months. Animals were divided into 2 groups, according to samples of screw dental implants used in the experiment: basic group - 12 dogs in which were used samples of the new construction of dental implant “Implant.uz” (Uzbekistan); control group – 12 dogs in which were applied screw dental implant system “Dentium” (South Korea). Terms of derivation of animals from experiments were 1, 3, and 6 months.By using morphological techniques were studied implants “Implant.uz” from medical titanium BT-1.00 brand, their interaction with bone of alveolar ridge. “Implant.uz” did not cause pathological changes in the bone tissue of alveolar ridges of jaws and could be an alternative to known dental implants.

scholarly journals Evaluation of histological changes in peri-implant bone tissue after ultrasound application at early healing stages

2018 ◽  
pp. 38-42 ◽  
Author(s):  
S.P. Rubnikovich ◽  
I.S. Khomich
Keyword(s):  
Bone Tissue ◽  
Dental Implants ◽  
Morphological Changes ◽  
Experimental Studies ◽  
Low Frequency ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Dental Implantation

The aim was to study the nature of the morphological changes over time in the bone tissue around the implants under the influence of low-intensity pulsed ultrasound in the experiment. Materials and methods. Experimental studies were carried out on rabbits of the chinchilla breed, with dental implants installed in the tibia – two experimental groups with ultrasound and one control group. The animals were withdrawn from the experiment at 1, 2, 4 and 8 weeks and histological examination of the sections of peri-implant tissues was carried out. Results. The processes of osseointegration of dental implants in all groups of animals occurred without disrupting the normal process sequence. In the early stages granulation tissue is formed, later it is replaced by fibrous-reticular and coarse-fibrous bone tissue, and at the end – by a more mature lamellar bone tissue. However, the timing and degree of maturation of bone tissue, as well as osseointegration of implants in groups using low-frequency low-intensity pulsed ultrasound and without it differed significantly. The study showed that ultrasound exposure to implants and surrounding tissues induces osteoreparation processes, stimulating neoangiogenesis in granulation and newly formed bone tissue. Conclusion. Application of ultrasound to implants and subsequent application to the peri-implant tissues during and after dental implantation promotes the formation of bone tissue, identical by the histostructure to the maternal bone.

scholarly journals Small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survival

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Aizhen Chen ◽  
Shijie Tang ◽  
Jiawei He ◽  
Xiangyu Li ◽  
Guohao Peng ◽  
...  
Keyword(s):  
Survival Rate ◽  
Regenerative Medicine ◽  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Umbilical Vein ◽  
Fat Graft ◽  
Control Group ◽  
Ki 67

Abstract Background Small extracellular vesicles (sEVs) with genetic information secreted by cells play a crucial role in the cellular microenvironment. In this study, our purpose is to explore the characteristics of the small extracellular vesicles of human adipose-derived mesenchymal stromal cells (hADMSC-sEVs) and studied the role of hADMSC-sEVs in improving the survival rate of grafted fat. Methods In the present study, we used the transmission electron microscopy, nano-tracking analysis, nanoflow surface protein analysis, and zeta potential value to identify sEVs. SEVs’ trajectory was traced dynamically to verify whether hADMSC-sEVs can be internalized into human umbilical vein endothelial cells (HUVECs) in vitro at different times. The angiogenic property of hADMSC-sEVs was observed by measuring the volume, weight, and histological analysis of the grafted fats in nude mouse models. Results Our research showed that the hADMSC-sEVs were sEVs with double-layer membrane structure and the diameter of which is within 30–150 nm. hADMSC-sEVs exert biological influence mainly through internalization into cells. Compared with the control group, the hADMSC-sEVs group had a significantly higher survival rate of grafted fat, morphological integrity, and a lower degree of inflammation and fibrosis. And immunohistochemistry showed that hADMSC-sEVs significantly increased the neovascularisation and the expression of CD34, VEGFR2, and Ki-67 in the graft tissue. Conclusions As a potential nanomaterial, hADMSC-sEVs have been explored in the field of cell-free application of stem cell technology. hADMSC-sEVs promoted the survival of grafted fats by promoting the formation of new blood vessels, which is another promising progress in the field of regenerative medicine. We believe that hADMSC-sEVs will have a broad application prospect in the field of regenerative medicine in the future.

scholarly journals Extracellular vesicles from human multipotent stromal cells protect against hearing loss after noise trauma in vivo

2020 ◽  
Vol 10 (8) ◽  
Author(s):  
Athanasia Warnecke ◽  
Jennifer Harre ◽  
Hinrich Staecker ◽  
Nils Prenzler ◽  
Dirk Strunk ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Noise Trauma

scholarly journals The influence of Extracellular Vesicles (Exosomes) of Mesenchymal Stromal Cells on Bone Tissue Regeneration

2019 ◽  
Vol 27 (2) ◽  
pp. 196-203
Author(s):  
I.V. Maiborodin ◽  
◽  
A.I. Shevela ◽  
V.V. Morozov ◽  
T.V. Mikheeva ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Tissue Regeneration ◽  
Stromal Cells ◽  
Bone Tissue Regeneration

scholarly journals Influence of Implant Material and Surface on Differentiation and Proliferation of Human Adipose-Derived Stromal Cells

2018 ◽  
Vol 19 (12) ◽  
pp. 4033 ◽  
Author(s):  
Susanne Jung ◽  
Lauren Bohner ◽  
Marcel Hanisch ◽  
Johannes Kleinheinz ◽  
Sonja Sielker
Keyword(s):  
Dental Implants ◽  
Stromal Cells ◽  
Soft Tissues ◽  
Rough Surfaces ◽  
Titanium Implants ◽  
Polished Surface ◽  
Adipose Derived Stromal Cells ◽  
Proliferation And Differentiation ◽  
Levene Test ◽  
Promising Source

For the guided regeneration of periimplant hard and soft tissues, human adipose-derived stromal cells (hADSC) seem to be a promising source for mesenchymal stromal cells. For this, the proliferation and differentiation of hADSC were evaluated on titanium and zirconia dental implants with different surface treatments. Results were compared to edaphic cells as human osteoblasts (hOB) and human gingival fibroblasts (HGF). Primary cells were cultured on (1) titanium implants with a polished surface (Ti-PT), (2) sandblasted and acid-etched titanium (Ti-SLA), (3) sandblasted and alkaline etched zirconia (ZrO2-ZLA) and (4) machined zirconia (ZrO2-M). The cell proliferation and differentiation on osteogenic lineage were assessed after 1, 7 and 14 days. Statistical analysis was performed by one-way ANOVA and a modified Levene test with a statistical significance at p = 0.05. PostHoc tests were performed by Bonferroni-Holm. Zirconia dental implants with rough surface (ZrO2-ZLA) showed the highest proliferation rates (p = 0.048). The osteogenic differentiation occurred early for zirconia and later for titanium implants, and it was enhanced for rough surfaces in comparison to polished/machined surfaces. Zirconia was more effective to promote the proliferation and differentiation of hADSCs in comparison to titanium. Rough surfaces were able to improve the biological response for both zirconia and titanium.

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