Characterization of an Aerosol-Based Photobioreactor for Cultivation of Phototrophic Biofilms

Phototrophic biofilms, in particular terrestrial cyanobacteria, offer a variety of biotechnologically interesting products such as natural dyes, antibiotics or dietary supplements. However, phototrophic biofilms are difficult to cultivate in submerged bioreactors. A new generation of biofilm photobioreactors imitates the natural habitat resulting in higher productivity. In this work, an aerosol-based photobioreactor is presented that was characterized for the cultivation of phototrophic biofilms. Experiments and simulation of aerosol distribution showed a uniform aerosol supply to biofilms. Compared to previous prototypes, the growth of the terrestrial cyanobacterium Nostoc sp. could be almost tripled. Different surfaces for biofilm growth were investigated regarding hydrophobicity, contact angle, light- and temperature distribution. Further, the results were successfully simulated. Finally, the growth of Nostoc sp. was investigated on different surfaces and the biofilm thickness was measured noninvasively using optical coherence tomography. It could be shown that the cultivation surface had no influence on biomass production, but did affect biofilm thickness.

Download Full-text

Characterization of microstructured fibre hole diameter and pitch with Fourier domain optical coherence tomography

31st European Conference on Optical Communications (ECOC 2005) ◽

10.1049/cp:20050421 ◽

2005 ◽

Author(s):

J.C. Jasapara

Keyword(s):

Optical Coherence Tomography ◽

Hole Diameter ◽

Fourier Domain ◽

Optical Coherence ◽

Microstructured Fibre

Download Full-text

Morpho-molecular ex vivo detection and grading of non-muscle-invasive bladder cancer using forward imaging probe based multimodal optical coherence tomography and Raman spectroscopy

The Analyst ◽

10.1039/c9an01911a ◽

2020 ◽

Vol 145 (4) ◽

pp. 1445-1456 ◽

Cited By ~ 1

Author(s):

Fabian Placzek ◽

Eliana Cordero Bautista ◽

Simon Kretschmer ◽

Lara M. Wurster ◽

Florian Knorr ◽

...

Keyword(s):

Raman Spectroscopy ◽

Optical Coherence Tomography ◽

Imaging System ◽

Ex Vivo ◽

Large Field ◽

Optical Coherence ◽

Fiber Optic Probe ◽

Optic Probe ◽

Muscle Invasive

Characterization of bladder biopsies, using a combined fiber optic probe-based optical coherence tomography and Raman spectroscopy imaging system that allows a large field-of-view imaging and detection and grading of cancerous bladder lesions.

Download Full-text

Characterization of Optical Coherence Tomography Images for Colon Lesion Differentiation under Deep Learning

Applied Sciences ◽

10.3390/app11073119 ◽

2021 ◽

Vol 11 (7) ◽

pp. 3119

Author(s):

Cristina L. Saratxaga ◽

Jorge Bote ◽

Juan F. Ortega-Morán ◽

Artzai Picón ◽

Elena Terradillos ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Deep Learning ◽

Data Augmentation ◽

Optical Biopsy ◽

Colon Polyps ◽

Optical Coherence ◽

Colon Lesion ◽

Patient Prognosis ◽

B Scan Images

(1) Background: Clinicians demand new tools for early diagnosis and improved detection of colon lesions that are vital for patient prognosis. Optical coherence tomography (OCT) allows microscopical inspection of tissue and might serve as an optical biopsy method that could lead to in-situ diagnosis and treatment decisions; (2) Methods: A database of murine (rat) healthy, hyperplastic and neoplastic colonic samples with more than 94,000 images was acquired. A methodology that includes a data augmentation processing strategy and a deep learning model for automatic classification (benign vs. malignant) of OCT images is presented and validated over this dataset. Comparative evaluation is performed both over individual B-scan images and C-scan volumes; (3) Results: A model was trained and evaluated with the proposed methodology using six different data splits to present statistically significant results. Considering this, 0.9695 (±0.0141) sensitivity and 0.8094 (±0.1524) specificity were obtained when diagnosis was performed over B-scan images. On the other hand, 0.9821 (±0.0197) sensitivity and 0.7865 (±0.205) specificity were achieved when diagnosis was made considering all the images in the whole C-scan volume; (4) Conclusions: The proposed methodology based on deep learning showed great potential for the automatic characterization of colon polyps and future development of the optical biopsy paradigm.

Download Full-text

Synthesis of a New Phosphonate-Based Sorbent and Characterization of Its Interactions with Lanthanum (III) and Terbium (III)

Polymers ◽

10.3390/polym13091513 ◽

2021 ◽

Vol 13 (9) ◽

pp. 1513

Author(s):

Yuezhou Wei ◽

Khalid A. M. Salih ◽

Mohammed F. Hamza ◽

Toyohisa Fujita ◽

Enrique Rodríguez-Castellón ◽

...

Keyword(s):

High Tech ◽

Low Grade ◽

Acid Solutions ◽

Hydrogel Beads ◽

Secondary Sources ◽

Composite Sorbents ◽

Synthesis Procedure ◽

New Generation ◽

Mild Acid

High-tech applications require increasing amounts of rare earth elements (REE). Their recovery from low-grade minerals and their recycling from secondary sources (as waste materials) are of critical importance. There is increasing attention paid to the development of new sorbents for REE recovery from dilute solutions. A new generation of composite sorbents based on brown algal biomass (alginate) and polyethylenimine (PEI) was recently developed (ALPEI hydrogel beads). The phosphorylation of the beads strongly improves the affinity of the sorbents for REEs (such as La and Tb): by 4.5 to 6.9 times compared with raw beads. The synthesis procedure (epicholorhydrin-activation, phosphorylation and de-esterification) is investigated by XPS and FTIR for characterizing the grafting route but also for interpreting the binding mechanism (contribution of N-bearing from PEI, O-bearing from alginate and P-bearing groups). Metal ions can be readily eluted using an acidic calcium chloride solution, which regenerates the sorbent: the FTIR spectra are hardly changed after five successive cycles of sorption and desorption. The materials are also characterized by elemental, textural and thermogravimetric analyses. The phosphorylation of ALPEI beads by this new method opens promising perspectives for the recovery of these strategic metals from mild acid solutions (i.e., pH ~ 4).

Download Full-text