scholarly journals Layer-By-Layer: The Case for 3D Bioprinting Neurons to Create Patient-Specific Epilepsy Models

Materials ◽  
2019 ◽  
Vol 12 (19) ◽  
pp. 3218 ◽  
Author(s):  
Natasha Antill-O’Brien ◽  
Justin Bourke ◽  
Cathal D. O’Connell
Keyword(s):  
Three Dimensional ◽  
3D Models ◽  
Patient Specific ◽  
Layer By Layer ◽  
3D Bioprinting ◽  
3D Structures ◽  
Neuronal Populations ◽  
Layer Deposition ◽  
Epilepsy Models ◽  
Ink Formulation

The ability to create three-dimensional (3D) models of brain tissue from patient-derived cells, would open new possibilities in studying the neuropathology of disorders such as epilepsy and schizophrenia. While organoid culture has provided impressive examples of patient-specific models, the generation of organised 3D structures remains a challenge. 3D bioprinting is a rapidly developing technology where living cells, encapsulated in suitable bioink matrices, are printed to form 3D structures. 3D bioprinting may provide the capability to organise neuronal populations in 3D, through layer-by-layer deposition, and thereby recapitulate the complexity of neural tissue. However, printing neuron cells raises particular challenges since the biomaterial environment must be of appropriate softness to allow for the neurite extension, properties which are anathema to building self-supporting 3D structures. Here, we review the topic of 3D bioprinting of neurons, including critical discussions of hardware and bio-ink formulation requirements.

A Prototype of a 3D Bioprinter

2015 ◽  
Vol 237 ◽  
pp. 221-226 ◽  
Author(s):  
Jakub Mielczarek ◽  
Grzegorz Gazdowicz ◽  
Jakub Kramarz ◽  
Piotr Łątka ◽  
Marcin Krzykawski ◽  
...  
Keyword(s):  
Control System ◽  
Drug Testing ◽  
Three Dimensional ◽  
Living Cells ◽  
Layer By Layer ◽  
3D Bioprinting ◽  
Innovative Method ◽  
Layer Deposition ◽  
Potential Applications ◽  
Technical Details

3D bioprinting is an innovative method of manufacturing three-dimensional tissue-like structures. The method is based on a layer-by-layer deposition of biocompatible materials successively forming a scaffold for living cells. The technology allows to fabricate complicated tissue morphology, including vascular-like networks. The range of potential applications of 3D bioprinting is immense: from drug testing, across regenerative medicine, to organ transplantation. In this paper, we describe a prototype of a 3D bioprinter utilizing gelatin methacrylate (GelMA) doped with a photoinitiator as the printing substance. Biological requirements for the material, its synthesis and application adequacy for the bioprinting process are discussed. Technical details of the mechanical construction of the bioprinter and its control system are presented

scholarly journals 3D PRINTING AND 3D BIOPRINTING TO USE FOR MEDICAL APPLICATIONS

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Milan Šljivić ◽  
Dragoljub Mirjanić ◽  
Nataša Šljivić ◽  
Cristiano Fragassa ◽  
Ana Pavlović
Keyword(s):  
3D Printing ◽  
Three Dimensional ◽  
3D Models ◽  
Physical Models ◽  
Layer By Layer ◽  
3D Bioprinting ◽  
Solid Objects ◽  
Modern Methods ◽  
Digital File

The Additive manufacturing 3D printing is a process of creating a three dimensional solid objects or rapid prototyping of 3D models from a digital file, which builds layer by layer. The 3D bioprinting is a form sophisticated of 3D printing technology involving cells and tissues for the production of tissue for regenerative medicine, which is also built layer by layer into the area of human tissue or organ. This paper defines the modern methods and materials of the AM, which are used for the development of physical models and individually adjusted implants for 3D printing for medical purposes. The main classification of 3D printing and 3D bioprinting technologies are also defined by typical materials and a field of application. It is proven that 3D printing and 3D bioprinting techniques have a huge potential and a possibility to revolutionize the field of medicine.

scholarly journals Embedded Multimaterial Extrusion Bioprinting

2017 ◽  
Vol 23 (2) ◽  
pp. 154-163 ◽  
Author(s):  
Marco Rocca ◽  
Alessio Fragasso ◽  
Wanjun Liu ◽  
Marcel A. Heinrich ◽  
Yu Shrike Zhang
Keyword(s):  
Gravitational Force ◽  
3D Model ◽  
Three Dimensional ◽  
Experimental Setup ◽  
Complex Structures ◽  
Layer By Layer ◽  
3D Bioprinting ◽  
Entire Structure ◽  
Layer Deposition ◽  
Further Development

Embedded extrusion bioprinting allows for the generation of complex structures that otherwise cannot be achieved with conventional layer-by-layer deposition from the bottom, by overcoming the limits imposed by gravitational force. By taking advantage of a hydrogel bath, serving as a sacrificial printing environment, it is feasible to extrude a bioink in freeform until the entire structure is deposited and crosslinked. The bioprinted structure can be subsequently released from the supporting hydrogel and used for further applications. Combining this advanced three-dimensional (3D) bioprinting technique with a multimaterial extrusion printhead setup enables the fabrication of complex volumetric structures built from multiple bioinks. The work described in this paper focuses on the optimization of the experimental setup and proposes a workflow to automate the bioprinting process, resulting in a fast and efficient conversion of a virtual 3D model into a physical, extruded structure in freeform using the multimaterial embedded bioprinting system. It is anticipated that further development of this technology will likely lead to widespread applications in areas such as tissue engineering, pharmaceutical testing, and organs-on-chips.

Combining patient-specific, digital 3D models with tele-education for adolescents with CHD

2021 ◽  
pp. 1-6
Author(s):  
David Liddle ◽  
Sheri Balsara ◽  
Karin Hamann ◽  
Adam Christopher ◽  
Laura Olivieri ◽  
...  
Keyword(s):  
Medical Condition ◽  
Three Dimensional ◽  
Medical Knowledge ◽  
3D Models ◽  
Patient Specific ◽  
Coarctation Of Aorta ◽  
Education Session ◽  
Post Intervention ◽  
Cardiac Defects ◽  
Transposition Of Great Arteries

Abstract Introduction: Adolescents with CHD require transition to specialised adult-centred care. Previous studies have shown that adolescents’ knowledge of their medical condition is correlated with transition readiness. Three-dimensional printed models of CHD have been used to educate medical trainees and patients, although no studies have focused on adolescents with CHD. This study investigates the feasibility of combining patient-specific, digital 3D heart models with tele-education interventions to improve the medical knowledge of adolescents with CHD. Methods: Adolescent patients with CHD, aged between 13 and 18 years old, were enrolled and scheduled for a tele-education session. Patient-specific digital 3D heart models were created using images from clinically indicated cardiac magnetic resonance studies. The tele-education session was performed using commercially available, web-conferencing software (Zoom, Zoom Video Communications Inc.) and a customised software (Cardiac Review 3D, Indicated Inc.) incorporating an interactive display of the digital 3D heart model. Medical knowledge was assessed using pre- and post-session questionnaires that were scored by independent reviewers. Results: Twenty-two adolescents completed the study. The average age of patients was 16 years old (standard deviation 1.5 years) and 56% of patients identified as female. Patients had a variety of cardiac defects, including tetralogy of Fallot, transposition of great arteries, and coarctation of aorta. Post-intervention, adolescents’ medical knowledge of their cardiac defects and cardiac surgeries improved compared to pre-intervention (p < 0.01). Conclusions: Combining patient-specific, digital 3D heart models with tele-education sessions can improve adolescents’ medical knowledge and may assist with transition to adult-centred care.

Evaluation of Spherical Parameterization Methods for Three-Dimensional Reconstruction of Medical Images

2013 ◽  
Vol 365-366 ◽  
pp. 1342-1349
Author(s):  
Xing Hui Wu ◽  
Zhi Xiu Hao
Keyword(s):  
Medical Images ◽  
Three Dimensional ◽  
3D Models ◽  
Mapping Method ◽  
Geometric Error ◽  
Patient Specific ◽  
Shape Modelling ◽  
Dimensional Reconstruction ◽  
Statistical Shape ◽  
Spherical Parameterization

The spherical parameterization is important for the correspondence problem that is a major part of statistical shape modelling for the reconstruction of patient-specific 3D models from medical images. In this paper, we present comparative studies of five common spherical mapping methods applied to the femur and tibia models: the Issenburg et al. method, the Alexa method, the Saba et al. method, the Praun et al. method and the Shen et al. method. These methods are evaluated using three sets of measures: distortion property, geometric error and distance to standard landmarks. Results show that the Praun et al. method performs better than other methods while the Shen et al. method can be regarded as the most reliable one for providing an acceptable correspondence result. We suggest that the area preserving property can be used as a sufficient condition while the angle preserving property is not important when choosing a spherical mapping method for correspondence application.

Digital Design and 3D Printing of Aortic Arch Reconstruction in HLHS for Surgical Simulation and Training

2018 ◽  
Vol 9 (4) ◽  
pp. 454-458 ◽  
Author(s):  
Sarah A. Chen ◽  
Chin Siang Ong ◽  
Nagina Malguria ◽  
Luca A. Vricella ◽  
Juan R. Garcia ◽  
...  
Keyword(s):  
3D Printing ◽  
Aortic Arch ◽  
Surgical Simulation ◽  
Three Dimensional ◽  
3D Models ◽  
Digital Design ◽  
Patient Specific ◽  
Aortic Arch Reconstruction ◽  
Arch Reconstruction ◽  
And Training

Purpose: Patients with hypoplastic left heart syndrome (HLHS) present a diverse spectrum of aortic arch morphology. Suboptimal geometry of the reconstructed aortic arch may result from inappropriate size and shape of an implanted patch and may be associated with poor outcomes. Meanwhile, advances in diagnostic imaging, computer-aided design, and three-dimensional (3D) printing technology have enabled the creation of 3D models. The purpose of this study is to create a surgical simulation and training model for aortic arch reconstruction. Description: Specialized segmentation software was used to isolate aortic arch anatomy from HLHS computed tomography scan images to create digital 3D models. Three-dimensional modeling software was used to modify the exported segmented models and digitally design printable customized patches that were optimally sized for arch reconstruction. Evaluation: Life-sized models of HLHS aortic arch anatomy and a digitally derived customized patch were 3D printed to allow simulation of surgical suturing and reconstruction. The patient-specific customized patch was successfully used for surgical simulation. Conclusions: Feasibility of digital design and 3D printing of patient-specific patches for aortic arch reconstruction has been demonstrated. The technology facilitates surgical simulation. Surgical training that leads to an understanding of optimal aortic patch geometry is one element that may potentially influence outcomes for patients with HLHS.

scholarly journals Three-Dimensional Bioprinting of Cartilage by the Use of Stem Cells: A Strategy to Improve Regeneration

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1749 ◽  
Author(s):  
Livia Roseti ◽  
Carola Cavallo ◽  
Giovanna Desando ◽  
Valentina Parisi ◽  
Mauro Petretta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Life Quality ◽  
Chemical Properties ◽  
Cartilage Tissue ◽  
Layer By Layer ◽  
Search Terms ◽  
Layer Deposition ◽  
Fine Control

Cartilage lesions fail to heal spontaneously, leading to the development of chronic conditions which worsen the life quality of patients. Three-dimensional scaffold-based bioprinting holds the potential of tissue regeneration through the creation of organized, living constructs via a “layer-by-layer” deposition of small units of biomaterials and cells. This technique displays important advantages to mimic natural cartilage over traditional methods by allowing a fine control of cell distribution, and the modulation of mechanical and chemical properties. This opens up a number of new perspectives including personalized medicine through the development of complex structures (the osteochondral compartment), different types of cartilage (hyaline, fibrous), and constructs according to a specific patient’s needs. However, the choice of the ideal combination of biomaterials and cells for cartilage bioprinting is still a challenge. Stem cells may improve material mimicry ability thanks to their unique properties: the immune-privileged status and the paracrine activity. Here, we review the recent advances in cartilage three-dimensional, scaffold-based bioprinting using stem cells and identify future developments for clinical translation. Database search terms used to write this review were: “articular cartilage”, “menisci”, “3D bioprinting”, “bioinks”, “stem cells”, and “cartilage tissue engineering”.

Custom Patient-Specific Three-Dimensional Printed Mitral Valve Models for Pre-Operative Patient Education Enhance Patient Satisfaction and Understanding

2019 ◽  
Vol 13 (3) ◽  
Author(s):  
Kay S. Hung ◽  
Michael J. Paulsen ◽  
Hanjay Wang ◽  
Camille Hironaka ◽  
Y. Joseph Woo
Keyword(s):  
Patient Education ◽  
Mitral Valve ◽  
Three Dimensional ◽  
3D Models ◽  
Patient Specific ◽  
Imaging Data ◽  
Anatomical Models ◽  
Mitral Valves ◽  
Flexible Polymer ◽  
3D Printed

In recent years, advances in medical imaging and three-dimensional (3D) additive manufacturing techniques have increased the use of 3D-printed anatomical models for surgical planning, device design and testing, customization of prostheses, and medical education. Using 3D-printing technology, we generated patient-specific models of mitral valves from their pre-operative cardiac imaging data and utilized these custom models to educate patients about their anatomy, disease, and treatment. Clinical 3D transthoracic and transesophageal echocardiography images were acquired from patients referred for mitral valve repair surgery and segmented using 3D modeling software. Patient-specific mitral valves were 3D-printed using a flexible polymer material to mimic the precise geometry and tissue texture of the relevant anatomy. 3D models were presented to patients at their pre-operative clinic visit and patient education was performed using either the 3D model or the standard anatomic illustrations. Afterward, patients completed questionnaires assessing knowledge and satisfaction. Responses were calculated based on a 1–5 Likert scale and analyzed using a nonparametric Mann–Whitney test. Twelve patients were presented with a patient-specific 3D-printed mitral valve model in addition to standard education materials and twelve patients were presented with only standard educational materials. The mean survey scores were 64.2 (±1.7) and 60.1 (±5.9), respectively (p = 0.008). The use of patient-specific anatomical models positively impacts patient education and satisfaction, and is a feasible method to open new opportunities in precision medicine.

3D-BIOPRINTING (Application of 3D printer for Organ Fabrication)

2015 ◽  
Vol 3 (5) ◽  
pp. 346
Author(s):  
Y. Aishwarya ◽  
B. Gourangi ◽  
K. Abhijeet
Keyword(s):  
Organ Transplant ◽  
Three Dimensional ◽  
Organ Shortage ◽  
3D Printer ◽  
Layer By Layer ◽  
3D Bioprinting ◽  
Successive Layer ◽  
Major Development ◽  
High Level ◽  
Advanced Computer

Chronic shortage of human organs for transplantation has become more problematic in spite of major development in transplant technologies. In 2009, only 27,996 (18%) of 154,324 patients received organs and 8,863 (25 per day) died while on the waiting list. As of early 2014, approximately 120,000 people in the U.S. were awaiting an organ transplant. The solution to this problem is 3D bio-printing. This technology may provide a unique and new opportunity where we can print 3D organs. It incorporates two technologies, tissue engineering and 3D printing. 3D bioprinting involves dispensing cells onto a biocompatible scaffold using a successive layer-by-layer approach to generate tissue-like three-dimensional structures. It uses instruction in the CAD file for formation of the object, high level computer programming and ability to build highly advanced computer systems, it offers hope for bridging the gap between organ shortage and transplantation needs.

scholarly journals Natural Polymers for 3D Bioprinting of Organs

2020 ◽  
pp. 30-40
Author(s):  
Galina Sroslova ◽  
Yuliya Zimina ◽  
Elena Nesmeyanova ◽  
Margarita Postnova
Keyword(s):  
Raw Materials ◽  
Sol Gel ◽  
Three Dimensional ◽  
Biologically Active ◽  
Artificial Organs ◽  
Gel Point ◽  
Layer By Layer ◽  
3D Bioprinting ◽  
Natural Polymers ◽  
Biological Origin

Three-dimensional (3D) bioprintingis a well-known promising technology for the production of artificial biological organs providing unprecedented versatility for manipulating cells and other biomaterials with precise control of their location in space. Over the past decade, a number of 3D bioprinting technologies have been developed. Unlike traditional manufacturing technologies, 3D bioprinting allows to produce individual or personalized fabric designs. This helps to deposit cells of the desired type with selected biomaterials and desired biologically active substances. Natural polymers play a leading role in maintaining cellular and biomolecular processes before, during, as well as after three-dimensional bioprinting. Polymers of biological origin can be extracted from natural raw materials by means of physical or chemical methods. These polymers are widely used as effective hydrogels for loading cells to form tissues, build a vascular, nervous, lymphatic network, and also to implement multiple biological, biochemical, physiological, biomedical and other functions. Any natural polymers that have a sol-gel phase transition (i.e., a gel point) under certain conditions can be printed using the automatic layer-by-layer deposition method. In fact, very few of them can be printed under various conditions (low temperature, without the help of physical, chemical, biochemical crosslinking of the incorporated polymer chains). Thus, not all natural polymers can meet all the basic requirements for 3D bioprinting. As a rule, natural polymers as the main component of various inks, which contain cells suspended in a specific medium, must meet several basic requirements for successful 3D bioprinting of organs, as well as clinical applications. These include biocompatibility, that is, non-toxic or without apparent toxicity; biodegradability (unlikenon-biodegradable polymers can be used as auxiliary structures); biostability with sufficiently high mechanical strength both at the time of processing and during operation; bioprinterness (workability). This review is devoted to modern research in the field of natural polymers used to print biological artificial organs.

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