scholarly journals 3D-Printed Barrier Membrane Using Mixture of Polycaprolactone and Beta-Tricalcium Phosphate for Regeneration of Rabbit Calvarial Defects

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3280
Author(s):  
Jun-Young Lee ◽  
Jin-Young Park ◽  
In-Pyo Hong ◽  
Su-Hee Jeon ◽  
Jae-Kook Cha ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Tissue Reaction ◽  
Synthetic Bone ◽  
Tissue Integration ◽  
Osteogenic Cells ◽  
Barrier Membrane ◽  
Beta Tricalcium Phosphate ◽  
Calvarial Defects ◽  
3D Printed ◽  
Good Biocompatibility

Background: Polycarprolactone and beta tricalcium phosphate (PCL/β-TCP) are resorbable biomaterials that exhibit ideal mechanical properties as well as high affinity for osteogenic cells. Aim: Objective of this study was to evaluate healing and tissue reaction to the PCL/β-TCP barrier membrane in the rabbit calvaria model for guided bone regeneration. Materials and Methods: The PCL/β-TCP membranes were 3D printed. Three circular defects were created in calvaria of 10 rabbits. The three groups were randomly allocated for each specimen: (i) sham control; (ii) PCL/β-TCP membrane (PCL group); and (iii) PCL/β-TCP membrane with synthetic bone graft (PCL-BG group). The animals were euthanized after two (n = 5) and eight weeks (n = 5) for volumetric and histomorphometric analyses. Results: The greatest augmented volume was achieved by the PCL-BG group at both two and eight weeks (p < 0.01). There was a significant increase in new bone after eight weeks in the PCL group (p = 0.04). The PCL/β-TCP membrane remained intact after eight weeks with slight degradation, and showed good tissue integration. Conclusions: PCL/β-TCP membrane exhibited good biocompatibility, slow degradation, and ability to maintain space over eight weeks. The 3D-printed PCL/β-TCP membrane is a promising biomaterial that could be utilized for reconstruction of critical sized defects.

3D printed polycaprolactone/beta-tricalcium phosphate/magnesium peroxide oxygen releasing scaffold enhances osteogenesis and implanted BMSCs survival in repairing the large bone defect

2021 ◽  
Author(s):  
Ziyue Peng ◽  
Chengqiang Wang ◽  
Chun Liu ◽  
Haixia Xu ◽  
Yihan Wang ◽  
...  
Keyword(s):  
3D Printing ◽  
Bone Defect ◽  
Tricalcium Phosphate ◽  
Large Bone Defect ◽  
Beta Tricalcium Phosphate ◽  
Defect Area ◽  
3D Printed ◽  
Magnesium Peroxide ◽  
Large Bone ◽  
Phosphate Magnesium

Fabricate a MgO2-contained scaffold by 3D printing to improve ischemia and hypoxia in bone defect area.

Melatonin decorated 3D-printed beta-tricalcium phosphate scaffolds promoting bone regeneration in a rat calvarial defect model

2019 ◽  
Vol 7 (20) ◽  
pp. 3250-3259 ◽  
Author(s):  
Yali Miao ◽  
Yunhua Chen ◽  
Xiao Liu ◽  
Jingjing Diao ◽  
Naru Zhao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Calvarial Defect ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
3D Printed ◽  
Rat Calvarial Defect

3D-printed β-TCP scaffolds decorated with melatonin via dopamine mussel-inspired chemistry enhance the osteogenesis and in vivo bone regeneration.

Fabrication and biological evaluation of porous β-TCP bioceramics produced using digital light processing

2021 ◽  
pp. 095441192110411
Author(s):  
SongFeng Xu ◽  
Hang Zhang ◽  
Xiang Li ◽  
XinXin Zhang ◽  
HuanMei Liu ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Femoral Condyle ◽  
Biological Evaluation ◽  
Mechanical Tests ◽  
Digital Light Processing ◽  
Beta Tricalcium Phosphate ◽  
Ectopic Bone ◽  
Good Biocompatibility ◽  
Mouse Osteoblast

Beta-tricalcium phosphate ( β-TCP) refers to one ideal bone repair substance with good biocompatibility and osteogenicity. A digital light processing (DLP)-system used in this study creates bioceramic green part by stacking up layers of photocurable tricalcium phosphate-filled slurry with various β-TCP weight fractions. Results show that the sintering shrinkage is anisotropic and the shrinkage vertically reaches over that horizontally. The obtained porous β-TCP parts have both macroporous outer structure and microporous inner structure, the macropore size is 400–600 μm and the micropore size is 500–1500 nm. The mechanical tests show that the porous β-TCP bioceramic’s compressive strength reaches 16.53 MPa. The cell culture confirmed that the porous β-TCP bioceramic is capable of achieving the effective attaching, growing, and proliferating pertained to mouse osteoblast cells. This study identified considerable blood vessels and significant ectopic bone forming obviously based on the histologically-related assessment when implanting to rabbit femoral condyle deficiency for 3 months. Thus, under high bioactive property and osteoinductivity, and large precision and mechanical strength that can be adjusted, the DLP printed porous β-TCP ceramics is capable of being promising for special uses of bones repairing.

scholarly journals Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic Promoter

2021 ◽  
Vol 22 (16) ◽  
pp. 9084
Author(s):  
Seokhwan Yun ◽  
Dami Choi ◽  
Dong-Jin Choi ◽  
Songwan Jin ◽  
Won-Soo Yun ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Formation ◽  
Tricalcium Phosphate ◽  
Bone Fractures ◽  
Critical Diameter ◽  
Micro Computed Tomography ◽  
Reconstruction Methods ◽  
Osteoblast Cell Line ◽  
Calvarial Defects ◽  
3D Printed

Bone formation and growth are crucial for treating bone fractures. Improving bone-reconstruction methods using autologous bone and synthetic implants can reduce the recovery time. Here, we investigated three treatments using two different materials, a bone-derived decellularized extracellular matrix (bdECM) and β-tricalcium phosphate (β-TCP), individually and in combination, as osteogenic promoter between bone and 3D-printed polycaprolactone scaffold (6-mm diameter) in rat calvarial defects (8-mm critical diameter). The materials were tested with a human pre-osteoblast cell line (MG63) to determine the effects of the osteogenic promoter on bone formation in vitro. A polycaprolactone (PCL) scaffold with a porous structure was placed at the center of the in vivo rat calvarial defects. The gap between the defective bone and PCL scaffold was filled with each material. Animals were sacrificed four weeks post-implantation, and skull samples were preserved for analysis. The preserved samples were scanned by micro-computed tomography and analyzed histologically to examine the clinical benefits of the materials. The bdECM–β-TCP mixture showed faster bone formation and a lower inflammatory response in the rats. Therefore, our results imply that a bdECM–β-TCP mixture is an ideal osteogenic promoter for treating fractures.

Rat Calvarial Bone Regeneration by 3D-Printed Beta-Tricalcium Phosphate Incorporating MicroRNA-200c

2020 ◽  
Author(s):  
Matthew T. Remy ◽  
Adil Akkouch ◽  
Li He ◽  
Steven Eliason ◽  
Mason E. Sweat ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Calvarial Bone ◽  
Beta Tricalcium Phosphate ◽  
3D Printed
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