scholarly journals Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil

Materials ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 400
Author(s):  
Katarzyna Wojtowicz ◽  
Teresa Steliga ◽  
Piotr Kapusta ◽  
Joanna Brzeszcz ◽  
Tomasz Skalski
Keyword(s):  
Petroleum Hydrocarbons ◽  
Rhodococcus Erythropolis ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Biodegradation Process ◽  
Polycyclic Aromatic ◽  
Benzene Toluene ◽  
Xylene Isomers ◽  
Monoaromatic Hydrocarbons ◽  
Petroleum Pollutants

Biodegradation is a method of effectively removing petroleum hydrocarbons from the natural environment. This research focuses on the biodegradation of aliphatic hydrocarbons, monoaromatic hydrocarbons such as benzene, toluene, ethylbenzene, and all three xylene isomers (BTEX) and polycyclic aromatic hydrocarbons (PAHs) as a result of soil inoculation with a biopreparation A1 based on autochthonous microorganisms and a biopreparation A1 with the addition of γ-PGA. The research used biopreparation A1 made of the following strains: Dietzia sp. IN133, Gordonia sp. IN138 Mycolicibacterium frederiksbergense IN53, Rhodococcus erythropolis IN119, Rhodococcus sp. IN136 and Pseudomonas sp. IN132. The experiments were carried out in laboratory conditions (microbiological tests, respirometric tests, and in semi-technical conditions (ex-situ prism method). The biodegradation efficiency was assessed on the basis of respirometric tests, chromatographic analyses and toxicological tests. As a result of inoculation of AB soil with the biopreparation A1 within 6 months, a reduction of total petroleum hydrocarbons (TPH) (66.03%), BTEX (80.08%) and PAHs (38.86%) was achieved and its toxicity was reduced. Inoculation of AB soil with the biopreparation A1 with the addition of γ-PGA reduced the concentration of TPH, BTEX and PAHs by 79.21%, 90.19%, and 51.18%, respectively, and reduced its toxicity. The conducted research has shown that the addition of γ-PGA affects the efficiency of the biodegradation process of petroleum pollutants, increasing the degree of TPH biodegradation by 13.18%, BTEX by 10.11% and PAHs by 12.32% compared to pure biopreparation A1.

scholarly journals Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 709 ◽  
Author(s):  
Teresa Steliga ◽  
Katarzyna Wojtowicz ◽  
Piotr Kapusta ◽  
Joanna Brzeszcz
Keyword(s):  
Polychlorinated Biphenyls ◽  
Mixed Culture ◽  
Petroleum Hydrocarbons ◽  
Rhodococcus Erythropolis ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Soil Toxicity ◽  
Bacterial Strains ◽  
Sterile Soil ◽  
Rhodococcus Sp

Biodegradation is one of the most effective and profitable methods for the elimination of toxic polychlorinated biphenyls (PCBs) and total petroleum hydrocarbons (TPH) from the environment. In this study, aerobic degradation of the mentioned pollutants by bacterial strains Mycolicibacterium frederiksbergense IN53, Rhodococcus erythropolis IN129, and Rhodococcus sp. IN306 and mixed culture M1 developed based on those strains at 1:1:1 ratio was analyzed. The effectiveness of individual strains and of the mixed culture was assessed based on carried out respirometric tests and chromatographic analyses. The Rhodococcus sp. IN306 turned out most effective in terms of 18 PCB congeners biodegradation (54.4%). The biodegradation index was decreasing with an increasing number of chlorine atoms in a molecule. Instead, the Mycolicobacterium frederiksbergense IN53 was the best TPH degrader (37.2%). In a sterile soil, contaminated with PCBs and TPH, the highest biodegradation effectiveness was obtained using inoculation with mixed culture M1, which allowed to reduce both the PCBs (51.8%) and TPH (34.6%) content. The PCBs and TPH biodegradation capacity of the defined mixed culture M1 was verified ex-situ with prism method in a non-sterile soil polluted with aged petroleum hydrocarbons (TPH) and spent transformer oil (PCBs). After inoculation with mixed culture M1, the PCBs were reduced during 6 months by 84.5% and TPH by 70.8% as well as soil toxicity was decreased.

scholarly journals Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity

2020 ◽  
Vol 85 (6) ◽  
pp. 821-830
Author(s):  
Aleksandra Zeradjanin ◽  
Jelena Avdalovic ◽  
Marija Ljesevic ◽  
Olivera Tesic ◽  
Srdjan Miletic ◽  
...  
Keyword(s):  
Humic Acids ◽  
Petroleum Hydrocarbons ◽  
Added Value ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Number Of Microorganisms ◽  
The Fourier Transform ◽  
The Relationship ◽  
Acid Structure ◽  
Qualitative Changes

Environmental pollution is a global problem, while bioremediation technology removes pollutants from the environment using microorganisms. This study was aimed at investigating how a bioremediation process affected soil humification. In soil polluted with petroleum and its derivatives that was submitted to bioremediation, besides the total petroleum hydrocarbons and the number of microorganisms, quantitative and qualitative changes of isolated humic acids were determined during the process. The bioremediation of 150 m3 of polluted soil lasted 150 days. The level of total petroleum hydrocarbons decreased by 86.6 %, while the level of humic acids increased by 26.5 %. The elemental analysis showed the reduction of C and the H/C ratio and the increase of O and the O/C ratio of isolated humic acids during the process. The ratio of absorbencies at 465 and 665 nm also increased. Based on this and the Fourier-transform infrared spectra, it was shown that the humic acids isolated at the end of bioremediation were enriched with oxygen functional groups and aromatic structures. This study provides one of the first insights into the relationship between bioremediation and humification, as well as evidence of how hydrocarbon-degrading microorganisms have a significant influence on changes to humic acid structure during bioremediation.

scholarly journals The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil

2009 ◽  
Vol 52 (4) ◽  
pp. 1043-1055 ◽  
Author(s):  
Adriano Pinto Mariano ◽  
Sérgio Henrique Rezende Crivelaro ◽  
Dejanira de Franceschi de Angelis ◽  
Daniel Marcos Bonotto
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Microbial Population ◽  
Diesel Oil ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Co2 Production ◽  
Soil Bioremediation ◽  
Negative Effects ◽  
Labile Carbon

This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days. Although an increase in the soil fertility and microbial population were obtained with the vinasse, it demonstrated not to be adequate to enhance the bioremediation efficiency of diesel oil contaminated soils. The addition of the vinasse in the contaminated groundwaters had negative effects on the biodegradation of the hydrocarbons, since vinasse, as a labile carbon source, was preferentially consumed.

SEASONAL DISTRIBUTION AND CONTAMINATION LEVELS OF TOTAL PETROLEUM HYDROCARBONS AND POLYCYCLIC AROMATIC HYDROCARBONS IN MARINE ENVIRONMENT AT ALANG[-]SOSIYA SHIP SCRAPPING YARD, GULF OF CAMBAY, INDIA

2005 ◽  
Vol 2005 (1) ◽  
pp. 149-160
Author(s):  
M. Srinivasa Reddy ◽  
Shaik Basha ◽  
H. V. Joshi ◽  
G. Ramachandraiah
Keyword(s):  
Summer Monsoon ◽  
Aromatic Hydrocarbons ◽  
Petroleum Hydrocarbons ◽  
Winter Season ◽  
Total Petroleum Hydrocarbons ◽  
Reference Station ◽  
Intertidal Sediments ◽  
Pollution Levels ◽  
Polycyclic Aromatic ◽  
Contamination Levels

ABSTRACT The present study assesses the distribution and contamination levels of total petroleum hydrocarbons (TPHCs) in surface seawaters during three successive seasons, (summer, monsoon and winter), and polyeyelie aromatic hydrocarbons (PAHs) in intertidal sediments along the worlds largest Alang-Sosiya ship-scrapping yard. The pollution levels at this coast are evaluated and compared with those at one reference station (Mahuva, 60 km away from this yard towards south) in this region and similar ship scrapping yards in Asia. The concentrations of TPHCs in seawater were high in winter season followed by summer and monsoon which were relatively, much higher, about six times in winter and five times in summer/monsoon as compared to the reference station at Mahuva. The TPHCs concentrations in the coastal waters of Alang-Sosiya region are nearly 10–100 times higher on compared with other regions. The levels of PAHs found in the Alang-Sosiya sediments are between four and nine times higher than these prescribed levels by OSPAR commission. The overall levels of PAHs show the low content in Alang-Sosiya compared to values reported from Chang Jiang in China while the concentrations were nearly three times higher than Aliaga in Turkey. The relationships between the content of PAHs and %TOM (total organic matter) in sediments are discussed.

scholarly journals Bioremediation of Soil Contaminated with Diesel using Biopile system

2018 ◽  
Vol 14 (3) ◽  
pp. 48-56
Author(s):  
Noor Mohsen Jabbar ◽  
Estabriq Hasan Kadhim ◽  
Alaa Kareem Mohammed
Keyword(s):  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Bacterial Culture ◽  
Atmospheric Temperature ◽  
Pilot Scale ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Inoculum ◽  
Amended Soil

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition).  Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.

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