scholarly journals Chemical Composition and In Vitro Cytotoxic and Antimicrobial Activities of the Essential Oil from Leaves of Zanthoxylum monogynum St. Hill (Rutaceae)

Medicines ◽  
2017 ◽  
Vol 4 (2) ◽  
pp. 31
Author(s):  
Fernanda Silva ◽  
Nara Santos ◽  
Renata Pascon ◽  
Marcelo Vallim ◽  
Carlos Figueiredo ◽  
...  
Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 453 ◽  
Author(s):  
Nhan Trong Le ◽  
Duc Viet Ho ◽  
Tuan Quoc Doan ◽  
Anh Tuan Le ◽  
Ain Raal ◽  
...  

The present study aimed to determine the antimicrobial activity and chemical composition of leaves-extracted essential oil of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le (L. domatiophorus), including antibacterial, antimycotic, antitrichomonas and antiviral effects. The essential oil was obtained using hydrodistillation, with an average yield of 0.34 ± 0.01% (v/w, dry leaves). There were 52 constituents as identified by GC/MS with available authentic standards, representing 96.74% of the entire leaves oil. The essential oil was comprised of three main components, namely viridiflorene (16.47%), (-)-δ-cadinene (15.58%) and γ-muurolene (8.00%). The oil showed good antimicrobial activities against several species: Gram-positive strains: Staphylococcus aureus (two strains) and Enterococcus faecalis, with Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) values from 0.25 to 1% (v/v); Gram-negative strains such as Escherichia coli (two strains), Pseudomonas aeruginosa (two strains) and Klebsiella pneumoniae, with MIC and MLC values between 2% and 8% (v/v); and finally Candida species, having MIC and MLC between 0.12 and 4% (v/v).Antitrichomonas activity of the oil was also undertaken, showing IC50, IC90 and MLC values of 0.008%, 0.016% and 0.03% (v/v), respectively, after 48h of incubation. The essential oil resultedin being completely ineffective against tested viruses, ssRNA+ (HIV-1, YFV, BVDV, Sb-1, CV-B4), ssRNA- (hRSVA2, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses with EC50 values over 100 µg/mL. This is the first, yet comprehensive, scientific report about the chemical composition and pharmacological properties of the essential oil in L. domatiophorus.


2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Megil J. McNeil ◽  
Roy B. R. Porter ◽  
Lawrence A. D. Williams

The chemical composition of the essential oil obtained from the aerial parts of Cleome serrata by hydrodistillation was analyzed by employing GC-FID, GC-MS and RI. Fourteen compounds comprising 90.4% of the total oil composition were characterized. The main components identified were ( Z)-phytol (53.0%) and di(2-ethylhexyl)-phthalate (DEHP) (14.7%). The oil was evaluated for its in vitro antimicrobial activities against nine pathogenic microorganisms using the filter paper disc diffusion method. Moderate antimicrobial activity was observed against five of the pathogens assayed. In addition, the essential oil was tested against the sweet potato weevil, Cylas formicarius elegantulus. Strong knockdown insecticidal activity was observed.


2008 ◽  
Vol 25 (No. 2) ◽  
pp. 81-89 ◽  
Author(s):  
A. Adiguzel ◽  
H. Ozer ◽  
H. Kilic ◽  
B. Cetin

The present work reports the <i>in vitro</i> antimicrobial activities of the essential oil and methanol extract from <i>Satureja hortensis</i> as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), &gamma;-terpinene (18.56%), carvacrol (13.98%), and <i>p</i>-cymene (8.97). The essential oil of <i>Satureja hortensis</i> exhibited the activity against 25 bacteria, 8 fungi, and a yeast, <i>C. albicans</i>; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 &micro;l/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.


Molecules ◽  
2012 ◽  
Vol 17 (8) ◽  
pp. 9540-9558 ◽  
Author(s):  
Dorsaf Ben Hassine ◽  
Manef Abderrabba ◽  
Yan Yvon ◽  
Ahmed Lebrihi ◽  
Florence Mathieu ◽  
...  

2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Ismail Amri ◽  
Laura De Martino ◽  
Aurelio Marandino ◽  
Hamrouni Lamia ◽  
Hanana Mohsen ◽  
...  

Aromatic plants can interfere in the Mediterranean ecosystem, mainly by the introduction in the environment of volatile compounds. For this reason, we studied the chemical composition and the possible phytotoxic and antimicrobial activities of the essential oil extracted from leaves of Tunisian Artemisia herba-alba Asso. The chemical composition of the essential oil, obtained by hydrodistillation, was analyzed by GC and GC-MS. In all, 24 compounds were identified. The main components were camphor (39.1.%), chrysanthenone (15.0%) and cis-thujone (7.8%). The essential oil was evaluated for its in vitro phytotoxic activity against germination and initial radical growth of Raphanus sativus L., Lepidium sativum L., Sinapis arvensis L., Triticum durum L. and Phalaris canariensis L. seeds. The radicle elongation of the five seeds was affected to different extents by the oil, while germination was not affected. The oil, when tested against eight selected bacterial strains, showed low antimicrobial activity. The chemical composition of the oil of A. herba-alba can help in the chemosystematics of this complex genus. However, the recorded biological activities seem to be neither ecologically nor medicinally significant.


2020 ◽  
Vol 10 ◽  
Author(s):  
Faiza Kaddour ◽  
Nadia Aissaoui ◽  
Mohammed El Amine Dib ◽  
Okkacha Bensaid ◽  
Alain Muselli

Background: Infectious diseases are responsible worldwide for millions of deaths a year. Antibiotics, which have saved so many lives and improved life expectancy, may become ineffective due to a worrying increase in bacterial resistance. Some of the appropriate actions that could be initiated to address this problem are to develop and search for new antimicrobial substances from medicinal plants, and combine antibiotics with antimicrobials agents isolated from a reservoir of bioactive natural product. Objectives: The purpose of this work was to study the chemical composition of the essential oil and hydrosol extract of Plumbago europaea, to evaluate their in-vitro antimicrobial activities and evaluate in-vitro combinatory antimicrobial effect of hydrosol extract with Gentamicin and Amphotericin B against a large panel of microorganisms in an effort to reduce their minimum effective dose and minimizing their side effects. Methods: The essential oil and hydrosol extract obtained from roots of Plumbago europaea were analyzed by GC/MS and tested for their antibacterial and antifungal activities against twelve different strains of microorganisms. The effectiveness, in-vitro, of the association between the hydrosol extract and both Gentamicin and Amphotericin B was also investigated using the checkerboard method. Results: The obtained results revealed that nine and four components, representing for 92.4 % and 97.4% of the total essential oil and hydrosol extract composition were identified, respectively and hydrosol extract was more active than the essential oil against all screened microorganisms, with interesting MIC values (19 μg/mL). An important effect of hydrosol extract was obtained in decreasing the MIC of Gentamicin and Amphotericin B in all tested combinations. Conclusion: The in-vitro combination of the hydrosol extract with Gentamicin and Amphotericin B led to substantial MIC reduction against all tested microorganisms. This combination can help to reduce the minimum effective dose of antimicrobial drugs used, which may help to decrease their side effects; and deliver these medicines with similar potency.


2008 ◽  
Vol 73 (7) ◽  
pp. 703-711 ◽  
Author(s):  
Branislava Lakusic ◽  
Mihailo Ristic ◽  
Violeta Slavkovska ◽  
Jelena Antic-Stankovic ◽  
Marina Milenkovic

The present paper describes the chemical composition and antimicrobial activity of the essential oil of the endemic species Satureja horvatii Silic, collected in Montenegro. The essential oil was obtained from the aerial parts of the plant by hydrodistillation and analyzed by GC-MS. From the 34 compounds representing 100 % of the oil, the major compound was the phenolic monoterpene thymol (63.37 %). The oil contained smaller amounts of g-terpinene (7.49 %), carvacrol methyl ether (4.92 %), carvacrol (4.67 %), p-cymene (4.52%), a-terpinene (1.81 %), borneol (1.58 %), a-thujene (1.56 %), b-caryophyllene (1.55 %) and b-myrcene (1.44 %). The antimicrobial activity of the essential oil of S. horvatii was evaluated using the agar diffusion and broth microdilution methods. The essential oil exhibited antimicrobial activity to varying degrees against all the tested strains. The maximum activity of S. horvatii oil was observed against Gram-positive bacteria (Micrococcus luteus, Staphylococcus epidermidis, Staphylococcus aureus and Enterococcus faecalis) and against the yeast (Candida albicans). The oil exhibited moderate activity against the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae and weak activity against Pseudomonas aeruginosa. This study confirms that the essential oil of S. horvatii possesses antimicrobial activities in vitro against medically important pathogens.


2017 ◽  
Vol 22 (4) ◽  
pp. 770-776 ◽  
Author(s):  
Kamiar Zomorodian ◽  
Mahmoodreza Moein ◽  
Keyvan Pakshir ◽  
Forough Karami ◽  
Zahra Sabahi

Resistance of many pathogens to available drugs is a global challenge and is leading to growing interest in natural alternative products. In this study, chemical composition and in vitro antibacterial and antifungal activities of the essential oil from Salvia mirzayanii were investigated. The chemical constituents of essential oil from S mirzayanii were analyzed by gas chromatography–mass spectrometry. The antimicrobial activity was determined by broth microdilution. The main identified compounds were 1,8-cineole (41.2 ± 1.3%), linalool acetate (11.0 ± 0.5%), and α-terpinyl acetate (6.0 ± 0.4%) (mL of essential oil/g of plant material). The MIC95 were 0.03 to 0.5 µL/mL and 16 to 128 µL/mL for gram-positive and gram-negative bacteria, respectively. These results indicated that Salvia mirzayanii essential oil significantly inhibited the growth of standard and clinically isolated tested yeasts by MIC50 0.03 to 1 µL/mL. Potent antibacterial and antifungal activities of Salvia mirzayanii essential oil may be considered in future study, particularly against antibiotic-resistant cases.


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