scholarly journals Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

2008 ◽  
Vol 25 (No. 2) ◽  
pp. 81-89 ◽  
Author(s):  
A. Adiguzel ◽  
H. Ozer ◽  
H. Kilic ◽  
B. Cetin
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Satureja Hortensis ◽  
Bacteria And Fungi

The present work reports the <i>in vitro</i> antimicrobial activities of the essential oil and methanol extract from <i>Satureja hortensis</i> as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), &gamma;-terpinene (18.56%), carvacrol (13.98%), and <i>p</i>-cymene (8.97). The essential oil of <i>Satureja hortensis</i> exhibited the activity against 25 bacteria, 8 fungi, and a yeast, <i>C. albicans</i>; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 &micro;l/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

scholarly journals In VitroAntimicrobial Activity of Essential Oil ofThymus schimperi,Matricaria chamomilla,Eucalyptus globulus, andRosmarinus officinalis

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Awol Mekonnen ◽  
Berhanu Yitayew ◽  
Alemnesh Tesema ◽  
Solomon Taddese
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Drug Preparation ◽  
Bacteria And Fungi

In this study, thein vitroantimicrobial activities of four plant essential oils (T. schimperi,E. globulus,R. officinalis, andM. Chamomilla) were evaluated against bacteria and fungi. The studies were carried out using agar diffusion method for screening the most effective essential oils and agar dilution to determine minimum inhibitory concentration of the essential oils. Results of this study revealed that essential oils ofT. schimperi,E. globulus, andR. officinaliswere active against bacteria and some fungi. The antimicrobial effect ofM. chamomillawas found to be weaker and did not show any antimicrobial activity. The minimum inhibitory concentration values ofT. schimperiwere<15.75 mg/mL for most of the bacteria and fungi used in this study. The minimum inhibitory concentration values of the other essential oils were in the range of 15.75–36.33 mg/mL against tested bacteria. This study highlighted the antimicrobial activity of the essential oil ofE. globulus,M. chamomilla,T. Schimperi, andR. officinalis. The results indicated thatT. schimperihave shown strong antimicrobial activity which could be potential candidates for preparation of antimicrobial drug preparation.

scholarly journals In Vitro Antimicrobial Activity of Essential Oil Extracted from Leaves of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le in Vietnam

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 453 ◽  
Author(s):  
Nhan Trong Le ◽  
Duc Viet Ho ◽  
Tuan Quoc Doan ◽  
Anh Tuan Le ◽  
Ain Raal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Antiviral Effects ◽  
Scientific Report ◽  
Main Components ◽  
Hiv 1

The present study aimed to determine the antimicrobial activity and chemical composition of leaves-extracted essential oil of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le (L. domatiophorus), including antibacterial, antimycotic, antitrichomonas and antiviral effects. The essential oil was obtained using hydrodistillation, with an average yield of 0.34 ± 0.01% (v/w, dry leaves). There were 52 constituents as identified by GC/MS with available authentic standards, representing 96.74% of the entire leaves oil. The essential oil was comprised of three main components, namely viridiflorene (16.47%), (-)-δ-cadinene (15.58%) and γ-muurolene (8.00%). The oil showed good antimicrobial activities against several species: Gram-positive strains: Staphylococcus aureus (two strains) and Enterococcus faecalis, with Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) values from 0.25 to 1% (v/v); Gram-negative strains such as Escherichia coli (two strains), Pseudomonas aeruginosa (two strains) and Klebsiella pneumoniae, with MIC and MLC values between 2% and 8% (v/v); and finally Candida species, having MIC and MLC between 0.12 and 4% (v/v).Antitrichomonas activity of the oil was also undertaken, showing IC50, IC90 and MLC values of 0.008%, 0.016% and 0.03% (v/v), respectively, after 48h of incubation. The essential oil resultedin being completely ineffective against tested viruses, ssRNA+ (HIV-1, YFV, BVDV, Sb-1, CV-B4), ssRNA- (hRSVA2, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses with EC50 values over 100 µg/mL. This is the first, yet comprehensive, scientific report about the chemical composition and pharmacological properties of the essential oil in L. domatiophorus.

scholarly journals Chemical Composition and Antifungal Activity of the Essential Oils of Schinus weinmannifolius Collected in the Spring and Winter

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Camila Hernandes ◽  
Silvia H. Taleb-Contini ◽  
Ana Carolina D. Bartolomeu ◽  
Bianca W. Bertoni ◽  
Suzelei C. França ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antimicrobial Action ◽  
Pharmacological Profile

Reports on the chemical and pharmacological profile of the essential oil of Schinus weinmannifolius do not exist, although other Schinus species have been widely investigated for their biological activities. This work aimed to evaluate the chemical composition and antimicrobial activity of the essential oil of S. weinmannifolius collected in the spring and winter. The essential oils were extracted by hydrodistillation, analyzed by GC/MS and submitted to microdilution tests, to determine the minimum inhibitory concentration. The oils displayed different chemical composition and antimicrobial action. Bicyclogermacrene and limonene predominated in the oils extracted in the winter and spring, respectively, whereas only the latter oil exhibited antifungal activity.

scholarly journals Chemical Composition and Antimicrobial Activities of the Essential Oils from Ocimum Selloi and Hesperozygis myrtoides

2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Márcia G. Martini ◽  
Humberto R. Bizzo ◽  
Davyson de L. Moreira ◽  
Paulo M. Neufeld ◽  
Simone N. Miranda ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Open Air Markets ◽  
Janeiro State

Ocimum selloi, a traditional medicinal plant from Brazil, is sold in open-air markets at Rio de Janeiro State. Hesperozygis myrtoides is a very aromatic small bush found in the State of Minas Gerais, Brazil, growing at an altitude of 1800m. The chemical composition of both essential oils was analyzed as well as their antimicrobial activity against fungi and bacteria. For all specimens of Ocimum selloi obtained at open-air markets, methylchavicol was major compound found (93.6% to 97.6%) in their essential oils. The major compounds identified in the oil of H. myrtoides were pulegone (44.4%), isomenthone (32.7%), and limonene (3.5%). Both oils displayed antimicrobial activity against all tested microorganisms but Candida albicans was the most susceptible one. Combinations of the two oils in different proportions were tested to verify their antimicrobial effect against C. albicans, which, however, was not modified in any of the concentrations tested. The minimum inhibitory concentration (MIC) was determined to confirm the antimicrobial activity against C. albicans as well as other clinical isolates ( C. glabrata, C. krusei, C. parapsilosis and C. tropicalis).

scholarly journals The In Vitro Antimicrobial Effects of Lavandula angustifolia Essential Oil in Combination with Conventional Antimicrobial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Stephanie de Rapper ◽  
Alvaro Viljoen ◽  
Sandy van Vuuren
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Lavandula Angustifolia ◽  
Gram Negative ◽  
Stock Solutions

The paper focuses on the in vitro antimicrobial activity of Lavandula angustifolia Mill. (lavender) essential oil in combination with four commercial antimicrobial agents. Stock solutions of chloramphenicol, ciprofloxacin, nystatin, and fusidic acid were tested in combination with L. angustifolia essential oil. The antimicrobial activities of the combinations were investigated against the Gram-positive bacterial strain Staphylococcus aureus (ATCC 6538) and Gram-negative Pseudomonas aeruginosa (ATCC 27858) and Candida albicans (ATCC 10231) was selected to represent the yeasts. The antimicrobial effect was performed using the minimum inhibitory concentration (MIC) microdilution assay. Isobolograms were constructed for varying ratios. The most prominent interaction was noted when L. angustifolia essential oil was combined with chloramphenicol and tested against the pathogen P. aeruginosa (ΣFIC of 0.29). Lavendula angustifolia essential oil was shown in most cases to interact synergistically with conventional antimicrobials when combined in ratios where higher volumes of L. angustifolia essential oil were incorporated into the combination.

scholarly journals Chemical composition and antimicrobial activity of the essential oil from Satureja horvatii Silic (Lamiaceae)

2008 ◽  
Vol 73 (7) ◽  
pp. 703-711 ◽  
Author(s):  
Branislava Lakusic ◽  
Mihailo Ristic ◽  
Violeta Slavkovska ◽  
Jelena Antic-Stankovic ◽  
Marina Milenkovic
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Micrococcus Luteus ◽  
Antimicrobial Activities ◽  
Maximum Activity ◽  
Moderate Activity ◽  
Aerial Parts ◽  
Weak Activity

The present paper describes the chemical composition and antimicrobial activity of the essential oil of the endemic species Satureja horvatii Silic, collected in Montenegro. The essential oil was obtained from the aerial parts of the plant by hydrodistillation and analyzed by GC-MS. From the 34 compounds representing 100 % of the oil, the major compound was the phenolic monoterpene thymol (63.37 %). The oil contained smaller amounts of g-terpinene (7.49 %), carvacrol methyl ether (4.92 %), carvacrol (4.67 %), p-cymene (4.52%), a-terpinene (1.81 %), borneol (1.58 %), a-thujene (1.56 %), b-caryophyllene (1.55 %) and b-myrcene (1.44 %). The antimicrobial activity of the essential oil of S. horvatii was evaluated using the agar diffusion and broth microdilution methods. The essential oil exhibited antimicrobial activity to varying degrees against all the tested strains. The maximum activity of S. horvatii oil was observed against Gram-positive bacteria (Micrococcus luteus, Staphylococcus epidermidis, Staphylococcus aureus and Enterococcus faecalis) and against the yeast (Candida albicans). The oil exhibited moderate activity against the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae and weak activity against Pseudomonas aeruginosa. This study confirms that the essential oil of S. horvatii possesses antimicrobial activities in vitro against medically important pathogens.

scholarly journals Chemical Characterization and In vitro Antimicrobial Activity of Caralluma europaea Essential Oil and Its Synergistic Potential with Conventional Antibiotics

2019 ◽  
pp. 1-11 ◽  
Author(s):  
Loubna Ait Dra ◽  
Abdellah Aghraz ◽  
Brahim Boualy ◽  
Saadia Oubaassine ◽  
Mustapha Barakate ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Aerial Part ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Acid Oxidation ◽  
Pharmaceutical Industries

Aim: This study was carried out to investigate the antioxidant and antimicrobial activities of essential oil from the aerial part of Caralluma europaea and to evaluate the synergistic potential between essential oil and antibiotics. Methodology: The chemical composition, antioxidant, antimicrobial activities and synergetic interaction between antimicrobial agents and essential oil isolated by hydrodistillation from the aerial part of C. europaea were evaluated. The chemical composition was analyzed by a Gas chromatography/mass spectrometry (GC/MS) system. Antioxidant activity was measured employing three methods: scavenging of free radical DPPH, reducing power assay and the inhibition of linoleic acid oxidation. The antimicrobial activity of essential oil against microbial strains was qualitatively and quantitatively assessed by the presence or absence of inhibition zones diameters, and MIC values. The in vitro association between essential oil and some commercial antibiotics was also investigated. Results: The GC/MS analysis shows that a total of 21 constituents were identified and the main compounds were Terpinolene (19.5%), α-Terpinene (16.2%) and Linalool (15.3%). Antioxidant study showed that essential oil exhibited antioxidant activity with IC50 values ranging from 0.32 mg/ml to 1.45 mg/ml. The results of antimicrobial activity showed that the essential oil had an inhibitory effect against the majority of tested microorganisms except K. pneumonia and  P. aeruginosa. Gram-positive bacteria were found to be more sensitive than Gram-negative ones. Furthermore, essential oil approved an interesting antifungal activity against yeast species. Out of 25 combinations tested 64% showed total synergism, 20% had a partial synergistic interaction and 16% showed no effect. The best synergistic effect was obtained with the combination essential oil-gentamycin. Conclusion: Our results are of a great importance and suggest that C. europaea essential oil contain bioactive compounds with antioxidant and antimicrobial properties with possible applications in the food and pharmaceutical industries.

scholarly journals Antimicrobial potential of Casearia sylvestris against oral bacteria

2016 ◽  
Vol 45 (4) ◽  
pp. 214-218 ◽  
Author(s):  
Amanda Henriques CAVALHEIRO ◽  
Ana Paula Terossi GODOI ◽  
Tatiane Cruz de CARVALHO ◽  
Alma Blasida CATIRSE ◽  
Niege Araçari Jacometti Cardoso FURTADO
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Oral Bacteria ◽  
Volatile Fraction ◽  
Casearia Sylvestris

Abstract Aim The aim of this study was to obtain Casearia sylvestris leave extracts by different extractive methods, including the obtention of essential oil, in order to compare their antimicrobial activities to conventional mouthwash chlorhexidine against oral bacteria. Material and method For this evaluation, extracts from the leaves were obtained by different methods of extraction (infusion, decoction, maceration and percolation) using different solvent systems: water 100%, ethanol 100%, methanol 100%, water: ethanol 3:7; water: ethanol 7:3; water: methanol 7:3 and water: methanol 3:7. The essential oil, which corresponds to a volatile fraction, was obtained by hydrodistillation using Clevenger modified apparatus. The microdilution broth method was used to determine the values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for the following microorganisms: Streptococcus mutans ATCC 25175, S. mitis ATCC 49456, S. sanguinis ATCC 10556, S. salivarius ATCC 25975, Lactobacillus casei ATCC 11578 and Enterococcus faecalis ATCC4082. Chlorhexidine gluconate was used as a positive control. Result All extracts evaluated in the used protocol displayed MIC values higher than 400 µg/mL and few showed bactericidal activity. The antimicrobial activity of essential oil was higher than the activity of the extracts, and the best minimum inhibitory concentration and minimum bactericidal concentration values were obtained against L. casei (MIC of 0.023 µg/mL and MBC of 0.046 µg/mL) and S. mutans (MIC of 25 µg/mL and MBC of 50 µg/mL), respectively. Conclusion The essential oil of Casearia sylvestris has significant antimicrobial activity against oral microorganisms.

scholarly journals Stereospecific ligands and their complexes, Part VIII: Antimicrobial activity of palladium(II) complexes with 0,0'-dialkyl esters of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl)-pentanoic acid

2012 ◽  
Vol 66 (3) ◽  
pp. 349-355 ◽  
Author(s):  
Dragoslav Ilic ◽  
Jelena Vujic ◽  
Ivana Radojevic ◽  
Olgica Stefanovic ◽  
Ljiljana Comic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
General Formula ◽  
Inhibitory Concentration ◽  
Pentanoic Acid ◽  
In Vitro Antimicrobial Activity ◽  
Microdilution Method ◽  
Vis Spectroscopy ◽  
Bacteria And Fungi

Palladium(II) complexes (1-4) of general formula [PdCl2(R2-S,S-eddp)] with bidentate N,N?-ligands, O,O'-dialkyl esters (R = ethyl, n-propyl, n-butyl and n-pentyl), of (S,S)-ethylenediamine-N,N?-di-2-(4-methyl)pentanoic acid (S,S-eddp) were prepared and characterized by microanalysis, infrared and UV/VIS spectroscopy. The ligands and its complexes were tested for their in vitro antimicrobial activity against 15 species of bacteria and fungi. Testing is performed by the microdilution method, with the minimum inhibitory concentration (MIC) and the minimum microbicidal concentration (MMC) being determined. The MIC values were in range from 4.9 ?g cm-3 to > 5000 ?g cm-3 while MMC values ranged from 78 ?g cm-3 to > 5000 ?g cm-3. Palladium(II) complexes [PdCl2(Ln)] (n = 1-4) have statistically significant higher activity than the corresponding ligands. The complex 4 displayed the strongest activity among all tested compounds.

scholarly journals Composition and antimicrobial activity of the essential oil from Galatella linosyris (L.) Rchb. f. (Asteraceae)

2012 ◽  
Vol 77 (5) ◽  
pp. 619-626 ◽  
Author(s):  
Dejan Godjevac ◽  
Ljubodrag Vujisic ◽  
Ivan Vuckovic ◽  
Vlatka Vajs ◽  
Marina Sokovic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Chemical Analysis ◽  
Essential Oil ◽  
Inhibitory Concentration

The investigation of chemical composition and antimicrobial activity of the essential oil of Galatella linosyris was presented. Chemical analysis (GC/MS, NMR) showed that sabinene (40%), ?-pinene (35.5%), ?-pinene (4.5%), limonene (4%), ?-muurolene (4%), and E-Caryophyllene (3.3%) were dominant components in this oil. The microdilution assays was used to evaluate minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC, MFC). G. linosyris essential oil exhibited better antifungal than antibacterial activity.

Sign in / Sign up

Export Citation Format

Share Document