scholarly journals A Transit Time-Resolved Microflow Cytometry-Based Agglutination Immunoassay for On-Site C-Reactive Protein Detection

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 109
Author(s):  
Jianxi Qu ◽  
Yushan Zhang ◽  
Mathieu Chenier ◽  
Chang-qing Xu ◽  
Lan Chen ◽  
...  
Keyword(s):  
Detection Limit ◽  
Transit Time ◽  
Protein Detection ◽  
Detection Accuracy ◽  
Agglutination Reaction ◽  
Detection Time ◽  
C Reactive Protein ◽  
Time Resolved ◽  
Reactive Protein ◽  
Detection Principle

An accurate and rapid microflow cytometry-based agglutination immunoassay (MCIA) suitable for on-site antibody or antigen detection was proposed. In this study, quantitative C-reactive protein (CRP) detection was chosen as a model assay in order to demonstrate the detection principle. The average transit time was employed to estimate the extent of the agglutination reaction and improve the detection accuracy as compared to the intensity-dependent methods. The detection time was less than 8 min. and only a 20 µL serum sample was needed for each test. The results showed a linear relationship between the average transit time of aggregates and CRP concentrations ranging from 0 to 1 µg/mL. The R2 of this relationship was 0.99. The detection limit of this technology was 0.12 µg/mL CRP. The system used for CRP detection can be extended to also monitor other clinically relevant molecules.

scholarly journals The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andreea Lorena Mateescu ◽  
Nicolae-Bogdan Mincu ◽  
Silvana Vasilca ◽  
Roxana Apetrei ◽  
Diana Stan ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Protein Complexes ◽  
C Reactive Protein ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Reactive Protein ◽  
In Vitro Diagnostic ◽  
The Stability ◽  
Positive Controls

AbstractThe purpose of the present study was to evaluate de influence of protein–sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive protein is a plasmatic protein used as a biomarker for the diagnosis of a series of health problems such as ulcerative colitis, cardiovascular diseases, metabolic syndrome, due to its essential role in the evolution of chronic inflammation. The sugar–protein interaction was investigated using steady state and time resolved fluorescence. The results revealed that there are more than two classes of tryptophan, with different degree of accessibility for the quencher molecule. Our study also revealed that sugar–protein complexes have superior thermostability, especially after gamma irradiation at 2 kGy, the protein being stable and functional even after 22 days exposure to 40 °C.

Myocardial Infarction Biomarker C‐reactive Protein Detection on Nanocomposite Aptasensor

2020 ◽  
Author(s):  
Jing Li ◽  
Haitao Li ◽  
Jinpeng Xu ◽  
Xingzhou Zhao ◽  
Shujiang Song ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Protein Detection ◽  
C Reactive Protein ◽  
Reactive Protein

Evaluation of 2-Methacryloyloxyethyl Phosphorylcholine Polymeric Nanoparticle for Immunoassay of C-Reactive Protein Detection

2004 ◽  
Vol 76 (9) ◽  
pp. 2649-2655 ◽  
Author(s):  
Jongwon Park ◽  
Shigeru Kurosawa ◽  
Junji Watanabe ◽  
Kazuhiko Ishihara
Keyword(s):  
Protein Detection ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Polymeric Nanoparticle

scholarly journals Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

2014 ◽  
Vol 05 (03) ◽  
pp. 153-158 ◽  
Author(s):  
Hanen Chammem ◽  
Imen Hafaid ◽  
Olivier Meilhac ◽  
Farid Menaa ◽  
Laurence Mora ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Plasma ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Protein Detection ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles

Sensors ◽  
2011 ◽  
Vol 11 (12) ◽  
pp. 11335-11342 ◽  
Author(s):  
Harri Härmä ◽  
Juha Toivonen ◽  
Juhani T. Soini ◽  
Pekka Hänninen ◽  
Wolfgang J. Parak
Keyword(s):  
Fluorescence Immunoassay ◽  
C Reactive Protein ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Semiconducting Nanoparticles ◽  
Reactive Protein

Disposable Polydimethylsiloxane/Silicon Hybrid Chips for Protein Detection

2004 ◽  
Author(s):  
Shifeng Li ◽  
David Fozdar ◽  
Dongbing Shao ◽  
Shaochen Chen ◽  
Pierre N. Floriano ◽  
...  
Keyword(s):  
Protein Detection ◽  
Ccd Camera ◽  
Multiplex Assay ◽  
Pdms Layer ◽  
C Reactive Protein ◽  
Fluorescent Assay ◽  
Reactive Protein ◽  
Agarose Beads ◽  
Fluid Network ◽  
Pressure Compensation

This paper presents disposable protein analysis chips with single and multiple chambers - constructed from poly (dimethylsiloxane) (PDMS) and silicon. The chips are composed of a multilayer stack of PDMS layers that sandwich a silicon microchip. This inner silicon chip features an etched array of microcavities hosting agarose beads. The sample is introduced into the fluid network in the top PDMS layer where it is directed to the bead chamber. After reaction of the analyte with the probe beads, signal generated on the beads is captured with a CCD camera, digitally processed, and analyzed. An established bead-based fluorescent assay for C-reactive protein (CRP) was used here to characterize these hybrid chips. The detection limit of the single chamber protein chip was found to be 1ng/mL. Additionally, using the back pressure compensation method, the signals from each of the four-chamber chip were found to be within 10% of each other. Moreover, the fabrication of the multiple-chamber chip may increase throughput and multiplex assay capacity.

Sign in / Sign up

Export Citation Format

Share Document