scholarly journals The Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode

Micromachines ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1337
Author(s):  
Leyla Karadurmus ◽  
Burcu Dogan-Topal ◽  
Sevinc Kurbanoglu ◽  
Afzal Shah ◽  
Sibel A. Ozkan
Keyword(s):  
Metal Nanoparticles ◽  
Interaction Mechanism ◽  
Differential Pulse ◽  
Layer By Layer ◽  
Screen Printed Electrodes ◽  
Calf Thymus ◽  
Binding Time ◽  
Guanine And Adenine ◽  
Metal Nanomaterials ◽  
Screen Printed

The screen-printed electrodes have gained increasing importance due to their advantages, such as robustness, portability, and easy handling. The manuscript presents the investigation of the interaction between double-strand deoxyribonucleic acid (dsDNA) and three anthracyclines: epirubicin (EPI), idarubicin (IDA), and doxorubicin (DOX) by differential pulse voltammetry on metal nanoparticles modified by screen-printed electrodes. In order to investigate the interaction, the voltammetric signals of dsDNA electroactive bases were used as an indicator. The effect of various metal nanomaterials on the signals of guanine and adenine was evaluated. Moreover, dsDNA/PtNPs/AgNPs/SPE (platinum nanoparticles/ silver nanoparticles/screen-printed electrodes) was designed for anthracyclines–dsDNA interaction studies since the layer-by-layer modification strategy of metal nanoparticles increases the surface area. Using the signal of multi-layer calf thymus (ct)-dsDNA, the within-day reproducibility results (RSD%) for guanine and adenine peak currents were found as 0.58% and 0.73%, respectively, and the between-day reproducibility results (RSD%) for guanine and adenine peak currents were found as 1.04% and 1.26%, respectively. The effect of binding time and concentration of three anthracyclines on voltammetric signals of dsDNA bases were also evaluated. The response was examined in the range of 0.3–1.3 ppm EPI, 0.1–1.0 ppm IDA and DOX concentration on dsDNA/PtNPs/AgNPs/SPE. Electrochemical studies proposed that the interaction mechanism between three anthracyclines and dsDNA was an intercalation mode.

Uric Acid Sensor Based on PEDOT:PSS Modified Screen-Printed Carbon Electrode Fabricated with a Simple Painting Technique

2021 ◽  
Vol 24 (2) ◽  
pp. 43-50
Author(s):  
Wulan Tri Wahyuni ◽  
Rudi Heryanto ◽  
Eti Rohaeti ◽  
Achmad Fauzi ◽  
Budi Riza Putra
Keyword(s):  
Uric Acid ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
Electrochemical Measurement ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Layer By Layer ◽  
Screen Printed Carbon Electrode ◽  
Limit Of Quantitation ◽  
Screen Printed

A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.

Simultaneous determination of guanine and adenine in human saliva with graphite sparked screen-printed electrodes

Talanta ◽  
2021 ◽  
pp. 123119
Author(s):  
Anastasios V. Papavasileiou ◽  
Maria G. Trachioti ◽  
Jan Hrbac ◽  
Mamas I. Prodromidis
Keyword(s):  
Simultaneous Determination ◽  
Human Saliva ◽  
Screen Printed Electrodes ◽  
Guanine And Adenine ◽  
Screen Printed

scholarly journals Electrochemically Reduced Graphene Oxide-Based Screen-Printed Electrodes for Total Tetracycline Determination by Adsorptive Transfer Stripping Differential Pulse Voltammetry

Sensors ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 76 ◽  
Author(s):  
Anabela S. Lorenzetti ◽  
Tania Sierra ◽  
Claudia E. Domini ◽  
Adriana G. Lista ◽  
Agustin G. Crevillen ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Differential Pulse Voltammetry ◽  
Reduced Graphene Oxide ◽  
Selective Adsorption ◽  
Differential Pulse ◽  
Screen Printed Electrodes ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide ◽  
Pulse Voltammetry ◽  
Screen Printed

Disposable electrochemically reduced graphene oxide-based (ERGO) screen-printed electrodes (SPE) were developed for the determination of total tetracyclines as a sample screening approach. To this end, a selective adsorption-detection approach relied on adsorptive transfer stripping differential pulse voltammetry (AdTDPV) was devised, where the high adsorption capacity and the electrochemical properties of ERGO were simultaneously exploited. The approach was very simple, fast (6 min.), highly selective by combining the adsorptive and the electrochemical features of tetracyclines, and it used just 10 μL of the sample. The electrochemical sensor applicability was demonstrated in the analysis of environmental and food samples. The not-fully explored AdTDPV analytical possibilities on disposable nanostructured transducers become a new tool in food and environmental fields; drawing new horizons for “in-situ” analysis.

scholarly journals A Voltammetric Electronic Tongue Based on Commercial Screen-printed Electrodes for the Analysis of Aminothiols by Differential Pulse Voltammetry

2017 ◽  
Vol 29 (6) ◽  
pp. 1559-1565 ◽  
Author(s):  
Clara Pérez-Ràfols ◽  
Ana Gómez ◽  
Núria Serrano ◽  
José Manuel Díaz-Cruz ◽  
Cristina Ariño ◽  
...  
Keyword(s):  
Differential Pulse Voltammetry ◽  
Electronic Tongue ◽  
Differential Pulse ◽  
Screen Printed Electrodes ◽  
Pulse Voltammetry ◽  
Voltammetric Electronic Tongue ◽  
Screen Printed

Derivatization of SWCNTs with cobalt phthalocyanine residues and applications in screen printed electrodes for electrochemical detection of thiocholine

2011 ◽  
Vol 56 (11) ◽  
pp. 3988-3995 ◽  
Author(s):  
Elena Jubete ◽  
Kamila Żelechowska ◽  
Oscar A. Loaiza ◽  
Pedro J. Lamas ◽  
Estibalitz Ochoteco ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Cobalt Phthalocyanine ◽  
Screen Printed Electrodes ◽  
Screen Printed
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