scholarly journals Using Rhizosphere Phosphate Solubilizing Bacteria to Improve Barley (Hordeum vulgare) Plant Productivity

2021 ◽  
Vol 9 (8) ◽  
pp. 1619
Author(s):  
Ana Ibáñez ◽  
Alba Diez-Galán ◽  
Rebeca Cobos ◽  
Carla Calvo-Peña ◽  
Carlos Barreiro ◽  
...  
Keyword(s):  
Dry Weight ◽  
Crop Productivity ◽  
Barley Plant ◽  
Phosphate Solubilizing Bacteria ◽  
Microbial Inoculants ◽  
Total Phosphorous ◽  
Pot Trials ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Vulgare Plant

On average less than 1% of the total phosphorous present in soils is available to plants, making phosphorous one of the most limiting macronutrients for crop productivity worldwide. The aim of this work was to isolate and select phosphate solubilizing bacteria (PSB) from the barley rhizosphere, which has other growth promoting traits and can increase crop productivity. A total of 104 different bacterial isolates were extracted from the barley plant rhizosphere. In this case, 64 strains were able to solubilize phosphate in agar plates. The 24 strains exhibiting the highest solubilizing index belonged to 16 different species, of which 7 isolates were discarded since they were identified as putative phytopathogens. The remaining nine strains were tested for their ability to solubilize phosphate in liquid medium and in pot trials performed in a greenhouse. Several of the isolated strains (Advenella mimigardefordensis, Bacillus cereus, Bacillus megaterium and Burkholderia fungorum) were able to significantly improve levels of assimilated phosphate, dry weight of ears and total starch accumulated on ears compared to non-inoculated plants. Since these strains were able to increase the growth and productivity of barley crops, they could be potentially used as microbial inoculants (biofertilizers).

scholarly journals THE EFFECT OF SALINITY TO ACTIVITY AND EFFECTIVITY PHOSPHATE SOLUBILIZING BACTERIA ON GROWTH AND PRODUCTION OF PADDY

2015 ◽  
Vol 2 (1) ◽  
pp. 609 ◽  
Author(s):  
Sri Widawati
Keyword(s):  
Bacillus Thuringiensis ◽  
Pseudomonas Fluorescens ◽  
Dry Weight ◽  
Phosphate Solubilizing Bacteria ◽  
Aerobacter Aerogenes ◽  
Randomized Design ◽  
Growth Activity ◽  
Growth Promoting ◽  
Completely Randomized Design ◽  
Phosphate Solubilizing

<p>This study aimed to determine the extent of phosphate solubilizing bacteria resistant to salinity and still be able to provide P for paddy plant. Research using completely randomized design with fertilizer treatments: <br />(A) Bakteri Aerobacter aerogenes + Azotobacter indicus (B) Bakteri Bacillus thuringiensis + B. megaterium <br />+ Pseudomonas fluorescens, (C) Bakteri Nocardia mesentrica + Spirillum lipoferum, (D) Mix bakteri Pseudomonas fluorescens, Bacillus thuringiensis, B. megaterium, Nocardia mesentrica, Aerobacter aerogenes, Spirillum lipoferum, dan Azotobacter indicus, and (E) control (whitout inoculant), and salinity (NaCl): (1) 0% (0 grams / 7 kg soil), (2) 0.1% (7 grams / 7 kg soil), (3) 0.2% (14 g / 7 kg soil), (4) 0.3% (21 g / 7 kg soil), and (5) 0 , 4% (28 g / 7 kg soil). Thirty and one hundred days after transplanting (DAT), and then measured plant height, number of tillers, number and dry weight of whole grain paddy. The results showed that 0,1 % (7 gram/7 kg tanah) salinity is very good for the growth, activity and effectiveness of phosphate solubilizing bacteria and production of paddy, but 0.4% salinity (28 gr/7kg land) is still safe on the growth, activity and effectiveness of phosphate solubilizing bacteria (Pseudomonas fluorescens, Bacillus thuringiensis, <br />B. megaterium, Nocardia mesentrica, Aerobacter aerogenes, spirillum lipoferum and Azotobacter indicus) as biofertilizer or growth promoting rhizobacteria on growth and production of paddy.</p><p><br /><strong>Keywords</strong>: Salinity, Phosphate solubilizing bacteria, Paddy</p>

scholarly journals Profiling of Plant Growth-Promoting Metabolites by Phosphate-Solubilizing Bacteria in Maize Rhizosphere

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1071
Author(s):  
Minchong Shen ◽  
Jiangang Li ◽  
Yuanhua Dong ◽  
Hong Liu ◽  
Junwei Peng ◽  
...  
Keyword(s):  
Plant Growth ◽  
Microbial Control ◽  
Agricultural Practices ◽  
Phosphate Solubilizing Bacteria ◽  
Mean Values ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Microbial Treatments

Microbial treatment has recently been attracting attention as a sustainable agricultural strategy addressing the current problems caused by unreasonable agricultural practices. However, the mechanism through which microbial inoculants promote plant growth is not well understood. In this study, two phosphate-solubilizing bacteria (PSB) were screened, and their growth-promoting abilities were explored. At day 7 (D7), the lengths of the root and sprout with three microbial treatments, M16, M44, and the combination of M16 and M44 (Com), were significantly greater than those with the non-microbial control, with mean values of 9.08 and 4.73, 7.15 and 4.83, and 13.98 and 5.68 cm, respectively. At day 14 (D14), M16, M44, and Com significantly increased not only the length of the root and sprout but also the underground and aboveground biomass. Differential metabolites were identified, and various amino acids, amino acid derivatives, and other plant growth-regulating molecules were significantly enhanced by the three microbial treatments. The profiling of key metabolites associated with plant growth in different microbial treatments showed consistent results with their performances in the germination experiment, which revealed the metabolic mechanism of plant growth-promoting processes mediated by screened PSB. This study provides a theoretical basis for the application of PSB in sustainable agriculture.

scholarly journals Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

2017 ◽  
Vol 66 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Jian Zhang ◽  
Peng Cheng Wang ◽  
Ling Fang ◽  
Qi-An Zhang ◽  
Cong Sheng Yan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Wood Flour ◽  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Sequence Comparisons ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

scholarly journals Isolation of Phosphate Solubilizing Bacteria and their Use for Plant Growth Promotion in Tomato Seedling and Plant

2018 ◽  
Vol 13 (2) ◽  
pp. 61-70
Author(s):  
Rajiv Pathak ◽  
Vipassana Paudel ◽  
Anupama Shrestha ◽  
Janardan Lamichhane ◽  
Dhurva. P. Gauchan
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
In Vitro Study ◽  
Dry Weight ◽  
Greenhouse Experiment ◽  
Phosphate Solubilizing Bacteria ◽  
Tomato Seedling ◽  
Phosphate Solubilizing ◽  
Phosphorous Compounds

Phosphorous (P) is an essential macronutrient and most soils contain high levels of P. However, its availability to plant is limited by rapid immobilization of phosphorous compounds to insoluble forms and hence plant available forms of P in soils are found in low amounts. Phosphate solubilizing bacteria provide an eco-friendly alternative to convert insoluble phosphates into plant available forms. In the present study, three phosphate solubilizing bacterial isolates (PB-1, PB-4 and VC-01) with visually significant phosphate solubilizing abilities were isolated from tomato rhizosphere soil. In-vitro study in pikovskaya’s agar revealed that isolate PB-1 had the highest phosphate solubilizing ability with a phosphate solubilizing index of 2.08±0.07 followed by isolate VC-01 (1.31±0.09) and PB-4 (1.24±0.08). Isolates were used as bacterial inoculum to assess their ability to promote tomato (Lycopersicon esculentum var. Srijana) seedling and plant growth in in-vitro and greenhouse experiment respectively. Isolate PB-4 showed best growth promotion in seedling assay whereas isolate PB-1 and VC-01 also promoted seedling growth compared to control. In greenhouse experiment however, isolates VC-01 and PB-1 significantly enhanced all parameters (shoot length, root length, shoot and root dry weight) compared to uninoculated control whereas isolate PB-4 had a positive effect on all parameters except root length.Kathmandu University Journal of Science, Engineering and TechnologyVol. 13, No. 2, 2017, page: 61-70

scholarly journals Isolasi Rhizobakteri dan Pengaruh Aplikasinya dengan Pupuk N-P terhadap Mutu Benih dan Pertumbuhan Bibit Tetua Betina Jagung

2018 ◽  
Vol 45 (3) ◽  
pp. 255
Author(s):  
Pitri Ratna Asih ◽  
Memen Surahman ◽  
Dan Giyanto
Keyword(s):  
Field Experiments ◽  
Seed Quality ◽  
Female Parent ◽  
Dry Weight ◽  
Hybrid Seed ◽  
Phosphate Solubilizing Bacteria ◽  
Isolation And Identification ◽  
Seed Field ◽  
P Fertilizer ◽  
Phosphate Solubilizing

Increased productivity of maize can be done with the use of high quality seeds from improved varieties such as hybrid seed. The objectives of this study were Increasing productivity of maize female parent is important in order to reduce the price of hybrid seed. The objectives of this study were to determine the nitrogen fixing bacteria compatible with phosphate solubilizing bacteria (PSB), and using those bacteria to increase physiological seed quality and seedling growth of maize female parent. The research consisted of laboratory and field experiments. Laboratory experiment for the isolation and identification of rhizobacteria resulted in 25 Azotobacter and 29 Actinomycetes non-pathogenic isolates capable of fixing nitrogen and PSB selected for compatibility tests were AB3, B28, P12, P14, P24, and P31. The compatibility test showed 25 pairs of BPF with Azotobacter and 16 pairs of BPF with Actinomycetes were mutually compatible. The BPF pair with Azotobacter or Actinomycetes P24-AzL7, P24-AzL9, B28-AcCKB4, P24-AcCKB9, P24-AcCKB20, and P24-AcCKW5 were able to increase the vigor index of hybrid maize female parent seed. Field experiment was arranged in a split plot design with three replications. The main plot was dosage of N-P fertilizer (0%, 25%, 50%, 75%, and 100% of recommendation dosage), and the subplot was 12 rhizobacteria treatments selected from 25 compatible pairs of BPF with Azotobacter and 16 pairs of BPF with Actinomycetes and 1 control. The application of compatible pairs of bacteria had a significant effect on plant height, the number of leaves at 3 and 4 weeks after planting and plant dry weight. However, the best treatment i.e. B28-AcCKB4 was not significantly different with the nutrient broth treatment (as control).<br /><br /><br />

scholarly journals Keanekaragaman Mikrob Fungsional Rizosfer Nanas dengan Berbagai Tingkat Produktivitas

2020 ◽  
Vol 25 (4) ◽  
pp. 584-591
Author(s):  
Aditya Dyah Utami ◽  
Suryo Wiyono ◽  
Rahayu Widyastuti ◽  
Priyo Cahyono
Keyword(s):  
Phytophthora Cinnamomi ◽  
Disease Incidence ◽  
Crop Productivity ◽  
Total Density ◽  
Phosphate Solubilizing Bacteria ◽  
Vegetative Phase ◽  
Randomized Sampling ◽  
Chitinolytic Bacteria ◽  
Phosphate Solubilizing ◽  
Potassium Solubilizing Bacteria

Functional microbes of rhizosphere play important roles in nutrient transformation and controlling disease as well as in supporting plant growth and development. However, there is no study on the role of functional microbes on pineapple productivity. The purpose of this study was to investigate the abundance and diversity of soil functional microbes at different growth phases at two levels of productivity and their correlations to disease incidence. The research process included sampling of pineapple rhizospheric soil from vegetative and generative phases pineapples at low and high plant productivity sites, observations of disease incidence, and isolations of functional microbes. Functional groups of bacteria were Azotobacter, phosphate-solubilizing bacteria, potassium-solubilizing bacteria, antibiotics-producing bacteria, IAA-producing bacteria, and chitinolytic bacteria. The soil sampling method was simple randomized sampling at 6 locations with an area of each location ± 5 ha with a depth of 20 cm. Rhizosphere were taken in plants grown in high productivity area (>60tons/ha) and low productivity area (<60 tons/ha) in vegetative and generative phases. The results showed that potassium-solubilizing bacteria, chitinolytic bacteria, and IAA-producing bacteria were more abundant during the generative phase compared to those during vegetative phase. While Azotobacter, phosphate-solubilizing bacteria, and antibiotic-producing bacteria were more predominant during vegetative phase at various crop productivy. Total density of microbes was higher in soil with high crop productivity than that in soil with low crop productivity. The abundance of chitinolytic bacteria and IAA-producing bacteria had negative correlation with disease caused by Erwinia chrysanthemi and Phytophthora cinnamomi. Keywords: chitinolytic bacteria, growth phase, IAA, pineapple disease

scholarly journals A Bacterium Isolated From Soil in a Karst Rocky Desertification Region Has Efficient Phosphate-Solubilizing and Plant Growth-Promoting Ability

2021 ◽  
Vol 11 ◽  
Author(s):  
Jinge Xie ◽  
Zongqiang Yan ◽  
Guifen Wang ◽  
Wenzhi Xue ◽  
Cong Li ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gluconic Acid ◽  
Lateral Roots ◽  
Utilization Efficiency ◽  
Phosphate Solubilizing Bacteria ◽  
Karst Rocky Desertification ◽  
Rocky Desertification ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

Phosphorus in the soil accessible to plants can easily be combined with calcium ion, the content of which is high in karst rocky desertification (KRD) regions, thereby resulting in a low utilization efficiency of phosphorus. The application of phosphate-solubilizing bacteria (PSB) from the KRD region would facilitate enhanced phosphate availability in the soil. In the present study, the strains belonging to Acinetobacter, Paraburkholderia, and Pseudomonas with efficient phosphate-solubilizing ability were isolated from fruit tree rhizosphere soils in KRD regions. Particularly, Acinetobacter sp. Ac-14 had a sustained and stable phosphate-solubilizing ability (439–448 mg/L, 48–120 h). Calcium carbonate decreased the phosphate-solubilizing ability in liquid medium; however, it did not affect the solubilization index in agar-solidified medium. When cocultivated with Arabidopsis thaliana seedling, Ac-14 increased the number of lateral roots, fresh weight, and chlorophyll content of the seedlings. Metabolomics analysis revealed that Ac-14 could produce 23 types of organic acids, majorly including gluconic acid and D-(-)-quinic acid. Expression of Ac-14 glucose dehydrogenase gene (gcd) conferred Pseudomonas sp. Ps-12 with a sustained and stable phosphate-solubilizing ability, suggesting that the production of gluconic acid is an important mechanism that confers phosphate solubilization in bacteria. Moreover, Ac-14 could also produce indole acetic acid and ammonia. Collectively, the isolated Ac-14 from KRD regions possess an efficient phosphate-solubilizing ability and plant growth-promoting effect which could be exploited for enhancing phosphorus availability in KRD regions. This study holds significance for the improvement of soil fertility and agricultural sustainable development in phosphorus-deficient KRD regions.

Production of Plant Growth Promoting Substances by Phosphate Solubilizing Bacteria Isolated from Vertisols

2007 ◽  
Vol 2 (3) ◽  
pp. 326-333 ◽  
Author(s):  
A. Vikram ◽  
H. Hamzehzarghani . ◽  
A.R. Alagawadi . ◽  
P.U. Krishnaraj . ◽  
B.S. Chandrashekar .
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilizing Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing
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