In Vitro Antimicrobial Activity of Essential Oils Against Salmonella enterica Serotypes Enteritidis and Typhimurium Strains Isolated from Poultry

Salmonella enterica serotype Enteritidis and S. enterica serotype Typhimurium are frequently present among poultry and are associated with outbreaks of human salmonellosis. The study investigated the in vitro antimicrobial activity of essential oils (EOs) obtained from Aloysia triphylla, Cinnamomum zeylanicum, Cymbopogon citratus, Litsea cubeba, Mentha piperita, Syzygium aromaticum against S. Enteritidis and S. Thyphimurium strains previously isolated from poultry. A 1:1 mixture of C. zeylanicum and S. aromaticum was also tested. The activity of all compounds was evaluated against the yeast Saccharomyces cerevisiae, commonly used as probiotic. The highest antibacterial activity was observed for C. zeylanicum (minimum inhibitory concentrations (MICs) ranging from 1.26 mg/mL to 0.63 mg/mL), S. aromaticum (MICs from 2.637 mg/mL to 0.164 mg/mL) and the mixture (MICs from 1.289 mg/mL to 0.322 mg/mL). No activity was recorded against S. cerevisiae. The results suggest a possible use of C. zeylanicum and S. aromaticum, alone or in combination, in the farm environment for disinfection and in poultry diet, combined with S. cerevisiae administration, for an integrated approach to avoid Salmonella intestinal colonization.

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Chemical Composition and in vitro Antimicrobial Activity of the Essential Oils of Two Helichrysum Species from Tanzania

Zeitschrift für Naturforschung C ◽

10.1515/znc-2004-5-614 ◽

2004 ◽

Vol 59 (5-6) ◽

pp. 368-372 ◽

Cited By ~ 52

Author(s):

Christos Bougatsos ◽

Olipa Ngassapa ◽

Deborah K. B. Runyoro ◽

Ioanna B. Chinou

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Pathogenic Fungi ◽

Caryophyllene Oxide ◽

In Vitro Antimicrobial Activity ◽

Aerial Parts ◽

Main Components

The chemical composition of the essential oils obtained from the aerial parts of Helichrysum cymosum and H. fulgidum, from Tanzania, were analyzed by GC and GC/MS. A total of sixty-five compounds, representing 92.4% and 88.2% of the two oils, respectively, were identified. trans-Caryophyllene, caryophyllene oxide, β-pinene, p-cymene, spathulenol and β- bourbonene were found to be the main components. Furthermore, the oils were tested against six gram (±) bacteria and three pathogenic fungi. It was found that the oil of H. fulgidum exhibited significant antimicrobial activity, while the oil of H. cymosum was not active at all.

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Chemical Composition And In-Vitro Antimicrobial Activity Of The Essential Oils Of Three Greek Achillea Species

Zeitschrift für Naturforschung C ◽

10.1515/znc-2002-3-415 ◽

2002 ◽

Vol 57 (3-4) ◽

pp. 287-290 ◽

Cited By ~ 72

Author(s):

Prokopios Magiatis ◽

Alexios-Leandros Skaltsounis ◽

Ioanna Chinou ◽

Serkos A. Haroutounian

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Pathogenic Fungi ◽

The Other ◽

Gram Negative ◽

In Vitro Antimicrobial Activity ◽

Ms Analysis

The chemical composition of the essential oils of Achillea holosericea, Achillea taygetea, Achillea fraasii was determined by GC/MS analysis. Among the ninety-five assayed constituents, camphor, borneol and 1,8-cineol were found to be the major components. The in-vitro antimicrobial activity of these essential oils was evaluated against six bacteria indicating that the first is totally inactive, while the other two possess moderate to strong activities mainly against the Gram negative strains. The essential oil of A. fraasii was also active against the tested pathogenic fungi

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In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates

Journal of Intercultural Ethnopharmacology ◽

10.5455/jice.20160331064446 ◽

2016 ◽

Vol 5 (3) ◽

pp. 212 ◽

Cited By ~ 24

Author(s):

Hercules Sakkas ◽

Panagiota Gousia ◽

Vangelis Economou ◽

Vassilios Sakkas ◽

Stefanos Petsios ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Clinical Isolates ◽

Drug Resistant ◽

Gram Negative ◽

In Vitro Antimicrobial Activity

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The Inhibitory Effect of Three Essential Oils on Candida rugosa Lipase: In Vitro and In Silico Studies

The Natural Products Journal ◽

10.2174/2210315508666181009112415 ◽

2020 ◽

Vol 10 (3) ◽

pp. 208-215 ◽

Cited By ~ 1

Author(s):

Talia Serseg ◽

Khedidja Benarous ◽

Mohamed Yousfi

Keyword(s):

Molecular Docking ◽

Essential Oils ◽

In Silico ◽

Candida Rugosa ◽

Inhibitory Effect ◽

Mentha Piperita ◽

Inhibition Mechanism ◽

Syzygium Aromaticum ◽

In Silico Studies

Background: Essential oils have been used for centuries. EOs are gaining increasing interest because of their acceptance by consumers and their safe status. For the first time, the effect of essential oils on the inhibition of lipases has been investigated in this work. Objective: We aimed in this study to investigate in vitro the inhibitory effects of the three essential oils of most used spices: Peppermint (Mentha piperita L.), cinnamon (Cinnamomum zeylanicum L.) and Cloves (Syzygium aromaticum L. Merr. et Perry) against Candida rugose lipase. In silico studies using molecular docking have been achieved to study the inhibition mechanism of major compounds of EO: menthol, carvacrol, eugenol and cinnamylaldehyde toward CRL. Methods: The inhibitory effect of three essential oils were determined by candida rugosa enzyme and pNP-L as substrate using spectrophotometry. Autodock vina was used for molecular docking with 50 runs. Results: We have found that these essential oils have a strong inhibitory effect with IC50 values 1.09, 1.78 and 1.13 mg/ml compared with Orlistat 0.06 mg/ml. The results show competitive inhibition for the three major compounds Menthol, Carvacrol and Eugenol with uncompetitive inhibition for Cinnamaldehyde. Different repetition ratios of hydrogen bonds and hydrophobic interactions were observed. The saved interactions were with His449, Ser209, Gly123, Gly124 and Phe344 for all molecules. Conclusion: These observations support using and considering essential oils and their major compounds as good sources for design new drugs to treat candidiasis and other diseases related to Lipases.

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In vitro antimicrobial activity of selected essential oils against endometritis-causing microorganisms in mares

Journal of Equine Veterinary Science ◽

10.1016/j.jevs.2021.103840 ◽

2021 ◽

pp. 103840

Author(s):

Fernando P. Lisboa ◽

Wendel P. Silvestre ◽

Jéssica O. Castro ◽

Gabriel V. Martins ◽

Lorenzo G.T.M. Segabinazzi ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

In Vitro Antimicrobial Activity

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Chemical Composition and in-vitro Antimicrobial Activity of Leaf Essential Oils of Senecio pedunculatus Edgew. and Nepeta coerulescens Maxim

Journal of Biologically Active Products from Nature ◽

10.1080/22311866.2021.1887762 ◽

2021 ◽

Vol 11 (1) ◽

pp. 1-10

Author(s):

Navadha Bhatt ◽

Navabha Joshi ◽

Kapil Ghai

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

In Vitro Antimicrobial Activity

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Chemical Composition and Nematicidal Properties of Sixteen Essential Oils—A Review

Plants ◽

10.3390/plants10071368 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1368

Author(s):

Trifone D’Addabbo ◽

Pinarosa Avato

Keyword(s):

Essential Oils ◽

Water Solution ◽

Mentha Piperita ◽

Chemical Compositions ◽

Syzygium Aromaticum ◽

Strong Activity ◽

Monarda Fistulosa ◽

Mortality Effect

Essential oils (EOs) can be a large source of new food-safe and healthy nematicidal products, due to their strong activity on crop pathogens and pests, including phytoparasitic nematodes, as well as to their low environmental persistence. This review summarizes the results from our 10-year studies on chemical features and nematicidal properties of 16 EOs with different botanical origins and compositions, i.e., the EOs from Artemisia herba-alba Asso (Asteraceae), Cinnamomum camphora (L.) J. Presl. and Cinnamomum verum J. Presl. (Lauraceae), Citrus aurantium L., Cinnamomum. sinensis L. Osbeck and Ruta graveolens L. (Rutaceae), Eucalyptus citriodora Hook, Eucalyptus globulus Labill. and Syzygium aromaticum (L.) Marry et Perry (Myrtaceae), Mentha piperita L., Monarda didyma L., Monarda. fistulosa L., Rosmarinus officinalis L. and Thymus satureioides Cosson (Lamiaceae), Pelargonium asperum Ehrh ex Willd (Geraniaceae) and Schinus molle L. (Anacardiaceae). All these EOs were chemically characterized and tested in vitro and/or in vivo for their activity against the phytoparasitic species Meloidogyne incognita Kofoid et White (Chitw.), Pratylenchus vulnus Allen et Jensen and Xiphinema index Thorne et Allen. Toxicity bioassays were conducted by exposing 2nd stage juveniles (J2) of M. incognita, mixed-age specimens of P. vulnus and adult females of X. index to 2–100 μg mL−1 concentrations of EOs or EO’s major constituents for 4–96 h and checking mortality effect after a further 24–72 h permanence in water. Egg hatchability bioassays consisted in exposing (24–48 h) M. incognita egg masses to 500–1000 mg mL−1 EO solutions followed by a 5-week hatching test in water. The in vivo experiments were undertaken in sandy soil strongly infested by M. incognita and treated with different doses of EOs, applied either in water solution or by fumigation. The effects of the treatments on nematode infestation on tomato and in soil were checked at the end of each experiment. Structure-activity relationships, as suggested by the different chemical compositions of tested EOs, were also highlighted. In agreement with literature data, our studies indicated that most of the tested EOs are highly suitable for the formulation of new safe nematicides, though still retarded by the lack of efficient stabilization processes and standardized EOs’ components and extraction techniques.

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