scholarly journals Prospective Approach to the Anaerobic Bioconversion of Benzo- and Dibenzothiophene Sulfones to Sulfide

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1736 ◽  
Author(s):  
Olga Senko ◽  
Olga Maslova ◽  
Marina Gladchenko ◽  
Sergey Gaydamaka ◽  
Argam Akopyan ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Biogas Production ◽  
Poly Vinyl Alcohol ◽  
Sulfur Recovery ◽  
Actual Problem ◽  
Sulfate Reducing ◽  
Pure Cell ◽  
Reducing Bacteria ◽  
First Time ◽  
Anaerobic Bioconversion

Sulfur recovery from organic molecules such as toxic sulfones is an actual problem, and its solution through the use of environmentally friendly and nature-like processes looks attractive for research and application. For the first time, the possible bioconversion of organic sulfones (benzo-and dibenzothiophene sulfones) to inorganic sulfide under anaerobic conditions with simultaneous biogas production from glucose within a methanogenesis process is demonstrated. Biogas with a methane content of 50.7%–82.1% was obtained without H2S impurities. Methanogenesis with 99.7%–100% efficiency and 97.8%–100% conversion of benzo- and dibenzothiophene sulfones (up to 0.45 mM) to inorganic sulfide were obtained in eight days by using a combination of various anaerobic biocatalysts immobilized in a poly(vinyl alcohol) cryogel. Pure cell cultures of sulfate-reducing bacteria and/or H2-producing bacteria were tested as additives to the methanogenic activated sludge. The immobilized activated sludge “enhanced” by bacterial additives appeared to retain its properties and be usable multiple times for the conversion of sulfones under batch conditions.

Interactions between filamentous sulfur bacteria, sulfate reducing bacteria and poly-P accumulating bacteria in anaerobic-oxic activated sludge from a municipal plant

1998 ◽  
Vol 37 (4-5) ◽  
pp. 599-603 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori ◽  
Edja. Kofi. Bosque-Hamilton
Keyword(s):  
Activated Sludge ◽  
Sulfate Reduction ◽  
Reduction Rate ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reduction Rate ◽  
Filamentous Bulking ◽  
Sulfate Reducing ◽  
Sulfur Bacteria ◽  
Sulfate Reduction Rates ◽  
Reducing Bacteria

The interactions between filamentous sulfur bacteria (FSB), sulfate reducing bacteria (SRB) and poly-P accumulating bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions were examined in batch experiments using return sludge (RAS) and settled sewage. Phosphate release and sulfate reduction occurred simultaneously under anaerobic conditions. SRB were more sensitive to temperature changes than PAB. SRB played an important role in the decomposition of propionate to acetate. When the sulfate reduction rates were high, there was a tendency for the maximum release of phosphate also to be high. This was explained by the fact that PAB utilized the acetate produced by SRB. Sulfur oxidizing bacteria were sensitive to temperature change. When the sulfate reduction rate was high, the sulfide oxidizing rate was also high and filamentous bulking occurred. The results showed that sulfate reduction was a cause of filamentous bulking due to Type 021N that could utilize reduced sulfur.

scholarly journals Production of biogas: relationship between methanogenic and sulfate-reducing microorganisms

2017 ◽  
Vol 12 (1) ◽  
pp. 82-91 ◽  
Author(s):  
Ivan Kushkevych ◽  
Monika Vítězová ◽  
Tomáš Vítěz ◽  
Milan Bartoš
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Trees ◽  
Biogas Production ◽  
Sulfate Reducing Bacteria ◽  
Quantitative Composition ◽  
Rrna Gene ◽  
Sulfate Reducing ◽  
Relationship Of ◽  
Reducing Bacteria

AbstractThe production of high-quality methane depends on many factors, including temperature, pH, substrate, composition and relationship of the microorganisms. The qualitative and quantitative composition of methanogenic and sulfate-reducing microorganisms and their relationship in the experimental bioreactors has never been studied. The aim of this research was to characterize, for the first time, the diversity of the methanogenic microorganisms and sulfate-reducing bacteria, and study their relationship and biogas production in experimental bioreactors. Amplification of 16S rRNA gene fragments was carried out. Purified amplicons were paired-end sequenced on an Illumina Mi-Seq platform. The dominant morphotypes of these microorganisms in the bioreactor were homologous (99%) by the sequences of 16S rRNA gene to theMethanosarcina,Thermogymnomonas,Methanoculleusgenera andArchaeondeposited in GenBank. Three dominant genera of sulfate-reducing bacteria,Desulfomicrobium,DesulfobulbusandDesulfovibrio, were detected in the bioreactor. The phylogenetic trees showing their genetic relationship were constructed. The diversity and number of the genera, production of methane, hydrogen sulfide and hydrogen in the bioreactor was investigated. This research is important for understanding the relationship between methanogenic microbial populations and other bacterial physiological groups, their substrate competition and, in turn, can be helpful for controlling methanogenesis in bioreactors.

scholarly journals Nested PCR and New Primers for Analysis of Sulfate-Reducing Bacteria in Low-Cell-Biomass Environments

2010 ◽  
Vol 76 (9) ◽  
pp. 2856-2865 ◽  
Author(s):  
Ludovic Giloteaux ◽  
Marisol Goñi-Urriza ◽  
Robert Duran
Keyword(s):  
Nested Pcr ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Direct Pcr ◽  
Sulfate Reducing ◽  
Cell Biomass ◽  
Low Levels ◽  
Reducing Bacteria ◽  
First Time

ABSTRACT New primers were designed for the amplification of dsrAB genes by nested PCR to investigate the diversity of sulfate-reducing prokaryotes (SRP) in environments with low bacterial cell density. The success of the nested PCR for the determination of SRP diversity was estimated by terminal-restriction fragment length polymorphism analysis in the Reigous, a small creek at an inactive mine (Carnoulès, France), which constitutes an extreme acidic arsenic-rich environment. Nested PCR limits were evaluated in dsrAB-rich sediments, and this technique was compared to direct PCR using either known primers (DSR1F/DSR4R) or new primers (dsr619AF/dsr1905BR). The comparison of clone libraries revealed that, even if the levels of diversity observed were not identical, nested PCR did not reduce the diversity compared to that of direct DSR1F/DSR4R PCR. Clone sequences were affiliated mainly with the Desulfobacteraceae and Desulfohalobiaceae families. Many sequences (∼30%) were related to a deeply branching lineage unaffiliated with any cultured SRP. Although this dsrAB cluster was found in all libraries, the new primers better amplified this lineage, providing more information on this unknown bacterial group. Thanks to these new primers in nested PCR, the SRP community from Carnoulès could be characterized. Specific SRP populations were obtained according to environmental characteristics. Desulfomicrobiaceae-related sequences were recovered in samples with low pH, low levels of dissolved oxygen, and high As content, while sequences belonging to the deeply branching group were found in a less extreme sample. Furthermore, for the first time, dsrAB sequences related to the latter group were recovered from freshwater.

Symbiosis and competition among sulfate reduction, filamentous sulfur, denitrification, and poly-p accumulation bacteria in the anaerobic-oxic activated sludge of a municipal plant

1996 ◽  
Vol 34 (5-6) ◽  
pp. 119-128 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori ◽  
E. J. Bosque-Hamilton
Keyword(s):  
Organic Acids ◽  
Activated Sludge ◽  
Sulfate Reduction ◽  
Sulfide Oxidation ◽  
Sulfate Reducing Bacteria ◽  
Sulfur Cycle ◽  
Sulfate Reducing ◽  
P Accumulation ◽  
Relationship Of ◽  
Reducing Bacteria

Symbiosis and competition were examined among sulfate reducing bacteria (SRB), filamentous sulfur bacteria (FSB), denitrification bacteria (DNB) and poly-P accumulation bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions. Batch experiments were carried out using settled sewage from the same plant as the substrate under several conditions. Under oxic conditions, both sulfate reduction and sulfide oxidation occurred simultaneously, making a symbiotic relationship of SRB and FSB for establishment of a sulfur cycle sustaining the energy requirements. Under anoxic conditions, denitrification was dominant because DNB outcompeted PAB and SRB for organic acids. Under anaerobic conditions, phosphate release and sulfate reduction occurred simultaneously. SRB produced for moles of acetate from four moles of propionate and/or unknown substances by reduction of three moles of sulfate. PAB competed with sulfate-reducing bacteria for organic acids such as propionate. However, PAB utilized acetate produced by SRB.

scholarly journals Rubidium chloride modulated the intestinal microbiota community in mice

2020 ◽  
Author(s):  
Qian Chen ◽  
Zhiguo He ◽  
Yuting Zhuo ◽  
Shuzhen Li ◽  
Wenjing Yang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Sulfate Reducing Bacteria ◽  
Community Diversity ◽  
Microbial Composition ◽  
Intestinal Microbes ◽  
Sulfate Reducing ◽  
Bacterial Microbiome ◽  
Reducing Bacteria ◽  
First Time

Abstract Background The intestinal microbiota plays an important role in host health. Although rubidium (Rb) has been used to study for depression and cancers, the interaction between intestinal microbial commensals and Rb is still unexplored. To gain the knowledge of the relationship between Rb and intestinal microbes, 51 mice receiving RbCl-based treatment and 13 untreated mice were evaluated of their characteristics and bacterial microbiome changes. Results The 16S ribosomal RNA gene sequencing of feces showed RbCl generally maintained the microbial community diversity, while the shifts in gut microbial composition were apparent after RbCl exposure for the first time. RbCl significantly enhanced the abundances of Rikenellaceae, Alistipes, Clostridium XlVa and sulfate-reducing bacteria including Deltaproteobacteria, Desulfovibrionales, Desulfovibrionaceae and Desulfovibrio. While, RbCl significantly inhibited the abundances of Tenericutes, Mollicutes, Anaeroplasmatales, Anaeroplasmataceae and Anaeroplasma lineages. Besides, with regarding to the composition of archaea, RbCl significantly enhanced the abundances of Crenarchaeota, Thermoprotei, Sulfolobales, Sulfolobaceae and Sulfolobus lineages. Conclusions These results revealed that enrichments of Clostridium XlVa, Alistipes and sulfate-reducing bacteria could act on brain-gut-microbiota axis by affecting serotonergic system and immune system. Therefore, it was likely that RbCl would have beneficial anti-effects on depression and cancers by modifying brain-gut-microbiota axis.

scholarly journals First Evidence for the Presence of a Hydrogenase in the Sulfur-Reducing Bacterium Desulfuromonas acetoxidans

1999 ◽  
Vol 181 (17) ◽  
pp. 5505-5508 ◽  
Author(s):  
Marianne Brugna ◽  
Wolfgang Nitschke ◽  
René Toci ◽  
Mireille Bruschi ◽  
Marie-Thérèse Giudici-Orticoni
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Energetic Metabolism ◽  
Sulfate Reducing ◽  
Desulfuromonas Acetoxidans ◽  
Reducing Bacteria ◽  
First Time

ABSTRACT Hydrogenases, which are ubiquitous in sulfate-reducing bacteria, were previously thought to be absent from Desulfuromonas acetoxidans. For the first time, a hydrogenase from the strict anaerobic sulfur-respiring bacterium D. acetoxidans, grown on ethanol-malate, was detected and enriched. To assay the role of the hydrogenase in the energetic metabolism of D. acetoxidans, we examined the reactivity of the enzyme with polyheme cytochromes from the same bacterium.

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