Antidiabetic Activity and Potential Mechanism of Amentoflavone in Diabetic Mice

Aim: To investigate the anti-diabetic activity of amentoflavone (AME) in diabetic mice, and to explore the potential mechanisms. Methods: Diabetic mice induced by high fat diet and streptozotocin were administered with amentoflavone for 8 weeks. Biochemical indexes were tested to evaluate its anti-diabetic effect. Hepatic steatosis, the histopathology change of the pancreas was evaluated. The activity of glucose metabolic enzymes, the expression of Akt and pAkt, and the glucose transporter type 4 (GLUT4) immunoreactivity were detected. Results: AME decreased the level of glucose, total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and glucagon, and increased the levels of high density lipoprotein cholesterol (HDL-C) and insulin. Additionally, AME increased the activity of glucokinase (GCK), phosphofructokinase-1 (PFK-1), and pyruvate kinase (PK), and inhibited the activity of glycogen synthase kinase-3 (GSK-3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6-phosphatase (G-6-Pase). Mechanistically, AME increased superoxide dismutase (SOD), decreased malondialdehyde (MDA), activation of several key signaling molecules including pAkt (Ser473), and increased the translocation to the sedimenting membranes of GLUT4 in skeletal muscle tissue. Conclusions: AME exerted anti-diabetic effects by regulating glucose and lipid metabolism, perhaps via anti-oxidant effects and activating the PI3K/Akt pathway. Our study provided novel insight into the role and underlying mechanisms of AME in diabetes.

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Differences in the Effects of Anthocyanin Supplementation on Glucose and Lipid Metabolism According to the Structure of the Main Anthocyanin: A Meta-Analysis of Randomized Controlled Trials

Nutrients ◽

10.3390/nu13062003 ◽

2021 ◽

Vol 13 (6) ◽

pp. 2003

Author(s):

Risa Araki ◽

Akira Yada ◽

Hirotsugu Ueda ◽

Kenichi Tominaga ◽

Hiroko Isoda

Keyword(s):

Lipid Metabolism ◽

Meta Analysis ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Resistance Index ◽

Lipoprotein Cholesterol ◽

Controlled Trials ◽

Oh Groups ◽

Chemical Structures ◽

Glucose And Lipid Metabolism

The effectiveness of anthocyanins may differ according to their chemical structures; however, randomized clinical controlled trials (RCTs) or meta-analyses that examine the consequences of these structural differences have not been reported yet. In this meta-analysis, anthocyanins in test foods of 18 selected RCTs were categorized into three types: cyanidin-, delphinidin-, and malvidin-based. Delphinidin-based anthocyanins demonstrated significant effects on triglycerides (mean difference (MD): −0.24, p < 0.01), low-density lipoprotein cholesterol (LDL-C) (MD: −0.28, p < 0.001), and high-density lipoprotein cholesterol (HDL-C) (MD: 0.11, p < 0.01), whereas no significant effects were observed for cyanidin- and malvidin-based anthocyanins. Although non-significant, favorable effects on total cholesterol (TC) and HDL-C were observed for cyanidin- and malvidin-based anthocyanins, respectively (both p < 0.1). The ascending order of effectiveness on TC and LDL-C was delphinidin-, cyanidin-, and malvidin-based anthocyanins, and the differences among the three groups were significant (both p < 0.05). We could not confirm the significant effects of each main anthocyanin on glucose metabolism; however, insulin resistance index changed positively and negatively with cyanidin- and delphinidin-based anthocyanins, respectively. Therefore, foods containing mainly unmethylated anthocyanins, especially with large numbers of OH groups, may improve glucose and lipid metabolism more effectively than those containing methylated anthocyanins.

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Hypoglycemic Effect of Momordica charantia L. Var. Abbreviata Ser. Protein Hydrolysate Prepared with Alcalase

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.195-196.324 ◽

2012 ◽

Vol 195-196 ◽

pp. 324-329

Author(s):

Xiao Qing Yuan ◽

Chao Yuan ◽

Hong Mei Liu

Keyword(s):

Protein Hydrolysate ◽

Low Density Lipoprotein ◽

Fasting Blood Glucose ◽

Density Lipoprotein ◽

Momordica Charantia ◽

Lipoprotein Cholesterol ◽

Hypoglycemic Effect ◽

Degree Of Hydrolysis ◽

Diabetic Mice ◽

Long Term Study

Momordica charantia L. Var. abbreviata Ser. protein hydrolysates (MCPH) were prepared by enzymatic hydrolysis using Alcalase 2.4L. The acute hypoglycemic effect of MCPH at different degree of hydrolysis (DH) was investigated in alloxan-induced diabetic mice. The result showed the MCPH at 11% DH had the highest hypoglycemic effect. In the long-term study, repeated oral administration of MCPH at 11% DH for 28 days significantly reduced the fasting blood glucose (FBG) level, serum total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-c) levels in diabetic mice. At the same time, MCPH markedly increased body weight, serum high density lipoprotein cholesterol (HDL-c) after 28 days of treatment.

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Association Between Cardiac Natriuretic Peptides and Lipid Profile: a Systematic Review and Meta-Analysis

Scientific Reports ◽

10.1038/s41598-019-55680-z ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Francesco Spannella ◽

Federico Giulietti ◽

Marica Bordicchia ◽

John C. Burnett ◽

Riccardo Sarzani

Keyword(s):

Systematic Review ◽

Lipid Profile ◽

Natriuretic Peptides ◽

Meta Analysis ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Lipoprotein Cholesterol ◽

Glucose And Lipid Metabolism ◽

Cardiac Natriuretic Peptides ◽

Metabolic Properties

AbstractCardiac natriuretic peptides (NPs) play a fundamental role in maintaining cardiovascular (CV) and renal homeostasis. Moreover, they also affect glucose and lipid metabolism. We performed a systematic review and meta-analysis of studies investigating the association of NPs with serum lipid profile. A PubMed and Scopus search (2005–2018) revealed 48 studies reporting the association between NPs and components of lipid profile [total cholesterol (TC), low-density lipoprotein cholesterol (LDLc), high-density lipoprotein cholesterol (HDLc) and triglycerides (TG)]. Despite high inconsistency across studies, NPs levels were inversely associated with TC [k = 32; pooled r = −0.09; I2 = 90.26%], LDLc [k = 31; pooled r = −0.09; I2 = 82.38%] and TG [k = 46; pooled r = −0.11; I2 = 94.14%], while they were directly associated with HDLc [k = 41; pooled r = 0.06; I2 = 87.94%]. The relationship with LDLc, HDLc and TG lost significance if only studies on special populations (works including subjects with relevant acute or chronic conditions that could have significantly affected the circulating levels of NPs or lipid profile) or low-quality studies were taken into account. The present study highlights an association between higher NP levels and a favorable lipid profile. This confirms and extends our understanding of the metabolic properties of cardiac NPs and their potential in CV prevention.

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Effect of Mangifera indica L. on hyperglycemia, hyperlipidemia and atherogenicity in diabetic mice

TAP CHI SINH HOC ◽

10.15625/0866-7160/v40n2.11617 ◽

2018 ◽

Vol 40 (2) ◽

pp. 168-176

Author(s):

Nguyen Thi Ai Lan ◽

Dai Thi Xuan Trang

Keyword(s):

Body Weight ◽

Mangifera Indica ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Lipoprotein Cholesterol ◽

Atherogenic Index ◽

Diabetic Mice ◽

Low Density Lipoprotein Cholesterol ◽

Young Leaves ◽

Alloxan Monohydrate

In this study, the antihyperglycemic and antihyperlipidemic effects of Mangifera indica L. (MIL) young leaves extract were investigated using diabetic mice models. Male Mus musculus L. mice were intratoripeneally injected by Alloxan monohydrate (AM) to induce diabetes and were observed for 28 days. The blood glucose, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL_C), low-density lipoprotein cholesterol (LDL_C) and atherogenic index were evaluated. The acute toxicity of MIL was determined at the dose of 1000, 2500, 5000 mg/kg body weight of normal mice. Diabetic mice were orally administrated with extract of MIL (150, 300, 450 mg/kg body weight/ twice a day). The results showed that the MIL significantly decreased the plasma glucose, TC, TG, LDL_C levels and atherogenic index and greatly elevated the HDL_C level in diabetic mice (p

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HEALING POTENCY OF HAEMATOCOCCUS PLUVIALIS EXTRACT FOR TREATING TYPE 2 DIABETES IN RATS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i1.15629 ◽

2016 ◽

Vol 9 (1) ◽

pp. 192

Author(s):

Farouk K. El-baz ◽

Hanan F. Aly ◽

Sayeda M. Abdo ◽

Safaa A. Saad

Keyword(s):

Haematococcus Pluvialis ◽

Histopathological Examination ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Ethanolic Extract ◽

Diabetic Rats ◽

Lipoprotein Cholesterol ◽

Antidiabetic Activity

Objective: The present study aims to evaluate the antidiabetic effect of ethanolic extract of Haematococcus pluvialis (H. pluvialis) in streptozotocin (STZ)-induced diabetic rats.Methods: The antidiabetic activity of H. pluvialis was investigated by the determination of glucose and insulin levels, aspartate (AST), alanine transaminases (ALT), lipid profile including total cholesterol (TC), triglycerides (TG), low-density lipoprotein-cholesterol (LDL-C) and high-density-lipoprotein-cholesterol (HDL-C). Histopathological examination of pancreas and liver were also carried out.Results: The results revealed that the levels of glucose, TC, TG, LDL-C as well as AST and ALT enzyme activities were increased significantly in diabetic rats. While, insulin and HDL-C levels decreased significantly in STZ-induced diabetic rats. The remediation of diabetic rats with H. pluvialis attenuated the elevated levels of glucose, TC, TG, LDL-C as well as AST and ALT activities in diabetic rats. Besides, it improved insulin, HDL-C levels, pancreas and hepatic architectures.Conclusion: H. pluvialis extract has a promising antidiabetic potency through attenuation of several metabolic disorders associated diabetes.

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