scholarly journals Phytochemical Composition and Biological Activity of Berries and Leaves from Four Romanian Sea Buckthorn (Hippophae Rhamnoides L.) Varieties

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1170 ◽  
Author(s):  
Adriana Criste ◽  
Adriana Cristina Urcan ◽  
Andrea Bunea ◽  
Flavia Roxana Pripon Furtuna ◽  
Neli Kinga Olah ◽  
...  

Hippophae rhamnoides L. is an important source of natural antioxidant and antimicrobial agents. Phytochemical compounds, antioxidant and antibacterial properties of berries, and leaf extracts from four Romanian sea buckthorn cultivars were investigated. Large differences in the content of total polyphenols and flavonoids between the varieties were observed. HPLC analysis of the polyphenolic compounds showed greater differences in content in leaves than in berries. This study confirmed that sea buckthorn leaves and berries are a rich source of phenolic compounds, especially quercetin derivatives and hydrocinnamic acid derivatives. Five carotenoid compounds were identified in the berries: lutein, zeaxanthin, β-cryptoxanthin, cis-β-carotene, and β-carotene. From the results obtained in this study, it can be stated that the varieties whose berries yielded the highest quantities of polyphenols, flavonoids, and antioxidant activity, can be ranked as follows: SF6 > Golden Abundant > Carmen > Colosal, and for leaf extracts the ranked order is SF6 > Golden Abundant > Colosal > Carmen. A strong correlation between the total flavonoid yield and antioxidant activity (r = 0.96), was observed. All extracts showed antibacterial activity against S. aureus, B. cereus, and P. aeruginosa, however extracts from berries were less potent than extracts from leaves.

Author(s):  
Chagnaadorj Rentsendavaa ◽  
Dóra Székély ◽  
Diána Furulyás ◽  
György Végvári ◽  
Faraja Gonelimali ◽  
...  

Sea Buckthorn (SB) juice is rich in biologically active compounds. It has considerable health benefits; thus, it can serve as functional food ingredient. The aim of this study was to determine and compare the total content of polyphenols, antioxidant capacity (FRAP), soluble solid content, pH value, β-carotene content and colour parameters (L*, a*, b*) in berry juice of cultivar 'Leikora' Sea Buckthorn (SB) (Hippophae rhamnoides L.). The treatment was made as control sample of Sea Buckthorn juice (C), and Sea Buckthorn juice with 0.5 % (P0.5); 1 % (P1); 2 % (P2) dried pomace of Sea Buckthorn. The samples were stored at room temperature for physicochemical analysis at interval of 2 months for a total period of 14 months.According to our results, the β-carotene (C to P2 was 3.71, 4.82, 5.49 and 6.52 mg 100mL−1) as well as the antioxidant capacity of the samples increased with the growth of the pomace content. During storage, degradation occurred in the polyphenol content and antioxidant capacity, but the β-carotene content increased. The increase of the β-carotene content was 94.6 % (C) and 32.7 % (P0.5). The smaller reduction in antioxidant compounds was the higher the sample of pomace content is. The FRAP and total polyphenol values measured during storage confirm that the pomace has antioxidant effect. There is a close correlation between the two parameters, including a positive correlation (r = 0.8614), which indicates that a significant part of the antioxidant capacity of buckthorn is due to the presence of different polyphenols.


2017 ◽  
Vol 27 (7) ◽  
pp. 1257-1265 ◽  
Author(s):  
Chi Heung Cho ◽  
Holim Jang ◽  
Migi Lee ◽  
Hee Kang ◽  
Ho Jjn Heo ◽  
...  

Author(s):  
Raluca Maria PARLOG ◽  
Dan Cristian VODNAR ◽  
Francisc Vasile DULF ◽  
Loredana LEOPOLD ◽  
Carmen SOCACIU

The carotenoids and chlorophylls from sea buckthorn (Hippophae rhamnoides L., ssp. Carpatica) berries, leaves and seeds were identified by HPLC –PDA and quantified by UV-VIS spectrometry. The HPLC chromatograms showed that among carotenoids, lutein (tR= 11.093 min) was the major compound followed by β carotene (tR= 25.04 min), zeaxanthin (tR= 11.51 min), small quantities of neoxanthin (tR= 6.93 min), violaxanthin (tR= 4.66 min) and traces of β cryptoxanthin (tR= 18.14 min).The identified chlorophylls were: chlorophyll a (tR= 17.55 min), chlorophyll b (tR =14.97 min) and pheophytin a (tR= 23.82 min). In accordance with the HPLC fingerprint, the chlorophyll content was: 0% in berries, 41.16 % in leaves and 16.36% in seeds. The total carotenoid concentrations were 293.59, 264.80 and 3.63 mg /kg fresh berries, leaves and seeds, respectively.


Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 847
Author(s):  
Anita Zapałowska ◽  
Natalia Matłok ◽  
Miłosz Zardzewiały ◽  
Tomasz Piechowiak ◽  
Maciej Balawejder

The aim of this research was to show the effect of the ozonation process on the quality of sea buckthorn (Hippophae rhamnoides L.). The quality of the ozonated berries of sea buckthorn was assessed. Prior to and after the ozone treatment, a number of parameters, including the mechanical properties, moisture content, microbial load, content of bioactive compounds, and composition of volatile compounds, were determined. The influence of the ozonation process on the composition of volatile compounds and mechanical properties was demonstrated. The ozonation had negligible impact on the weight and moisture of the samples immediately following the treatment. Significant differences in water content were recorded after 7 days of storage. It was shown that the highest dose of ozone (concentration and process time) amounting to 100 ppm for 30 min significantly reduced the water loss. The microbiological analyses showed the effect of ozone on the total count of aerobic bacteria, yeast, and mold. The applied process conditions resulted in the reduction of the number of aerobic bacteria colonies by 3 log cfu g−1 compared to the control (non-ozonated) sample, whereas the number of yeast and mold colonies decreased by 1 log cfu g−1 after the application of 100 ppm ozone gas for 30 min. As a consequence, ozone treatment enhanced the plant quality and extended plant’s storage life.


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