scholarly journals Binding Characteristics Study of DNA based Aptamers for E. coli O157:H7

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 204
Author(s):  
Saika Siddiqui ◽  
Jie Yuan
Keyword(s):  
Comparative Study ◽  
Binding Affinity ◽  
Rapid Detection ◽  
Binding Capacity ◽  
Comprehensive Analysis ◽  
Binding Characteristics ◽  
E Coli ◽  
Fast Detection ◽  
The Common ◽  
Binding Capability

E. coli O157:H7 is a pathogenic bacterium producing verotoxins that could lead to serious complications such as hemolytic uremia syndrome. Fast detection of such pathogens is important. For rapid detection, aptamers are quickly gaining traction as alternative biorecognition molecules besides conventional antibodies. Several DNA aptamers have been selected for E. coli O157:H7. Nonetheless, there has not been a comparative study of the binding characteristics of these aptamers. In this work, we present a comprehensive analysis of binding characteristics including binding affinity (Kd) and binding capacity (Bmax) of DNA-based aptamers for E. coli O157:H7 using qPCR. Our results show that aptamer E18R has the highest binding capacity to E. coli 157:H7 and the highest specificity over non-pathogenic E. coli strains K12 and DH5α. Our study also finds that the common biotin-tag modification at 5′ end typically changes the binding capacity significantly. For most of the selected aptamers, the binding capacity after a biotin-tag modification decreases. There exists a discrepancy in the binding capability between the selected aptamer and the aptamer used for detection. Our study also shows that a lower concentration of Mg2+ ions in the binding buffer leads to a decrease in the binding capacity of E17F and E18R, while it does not affect the binding capacity of S1 and EcoR1.

Fast detection of E. coli by a novel fluorescent biosensor based on a FRET system between UCNPs and GO@Fe3O4 in urine specimens

2021 ◽  
Author(s):  
Yuan Yao ◽  
Guoming Xie ◽  
Xin Zhang ◽  
Jinshan Yuan ◽  
Yulei Hou ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
E Coli ◽  
Fast Detection ◽  
Fluorescent Biosensor ◽  
Turn On ◽  
Urine Specimens

Biosensors based on nanomaterials are becoming a research hotspot for the rapid detection of pathogenic bacteria. Herein, a “turn-on” fluorescent biosensor based on a FRET system was constructed for the...

COMPARATIVE STUDY ON GONO GINI'S ASSETS DUE TO A DEATH ACCORDING TO THE ISLAMIC LAW AND POSITIVE LAW

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Pardan Syafrudin
Keyword(s):  
Comparative Study ◽  
Common Property ◽  
Islamic Law ◽  
Positive Law ◽  
Community Property ◽  
Communal Property ◽  
Islamic Jurisprudence ◽  
Legal Restriction ◽  
The Common ◽  
Husband And Wife

The Common properties (community property) is an asset that the husband and wife acquired during the household lifes, which both of them is agree that after united through marriage bonds, that the property produced by one or both of them will be common property. It shows, that if there's an agreement between husband and wife before marriage (did not to unify their property), then the property produced both will not become a joint treasure. Thus, if a husband or wife dies, or divorces, then the property owned by both of them can be distributed in accordance with their respective shares, another case when the two couples are not making an agreement, then the property gained during marriage bonds can be divided into types of communal property. In Islamic law, this kind of treasure is not contained in the Qur'an or Sunnah. Nor in Islamic jurisprudence. However, Islamic law legalizes the existence of common property as long as it is applicable in a society and the benefit in the distribution of such property. In contrast to the positive law, this property types have been regulated and described in the Marriage Law, as well as the Islamic Law Compilations, which became the legal restriction in the affairs of marriage in force in Indonesia. In this study, the author tries to compile the existence of common property according to the Islamic law reviews and positive law.

Results of a comparative study of the use of fodder worms of two breeds for pool growing of young tilapy and clary catfish in the conditions of the Almaty

2020 ◽  
pp. 73-80
Author(s):  
Z. Bolatbekova ◽  
S. Assylbekova ◽  
B. Kulatayev ◽  
S. Koishybayeva ◽  
N. Bulavina
Keyword(s):  
Comparative Study ◽  
Biochemical Composition ◽  
Nutritional Value ◽  
Fish Farming ◽  
Biological Indicators ◽  
Comprehensive Analysis ◽  
Fish Feed ◽  
Fish Breeding ◽  
Breeding Zone ◽  
Improved Methods

The aryicle presents the results of a comparative study of the biochemical composition of earthworms of dendrobene and prospector rocks, the peculiarities of their cultivation and their use for feeding when growing juvenile tilapia and clary catfish. The experience of cultivating earthworms of two breeds using the improved methods of foreign authors is described. The results of a study of the biochemical composition of cultivated objects that determine their nutritional value for feeding fish are presented. The fish-breeding and biological indicators of juvenile tilapia and clary catfish, obtained during the cultivation of dendrobene and prospector in fish farming in the Almaty region (VI fish-breeding zone) during worm feeding, are analyzed. A comprehensive analysis of the studied indicators made it possible to assign a certain rating place to each worm breed in relation to its use as fish feed.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

scholarly journals Lowering DNA binding affinity of SssI DNA methyltransferase does not enhance the specificity of targeted DNA methylation in E. coli

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Krystyna Ślaska-Kiss ◽  
Nikolett Zsibrita ◽  
Mihály Koncz ◽  
Pál Albert ◽  
Ákos Csábrádi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Dna Binding ◽  
Binding Affinity ◽  
Dna Methyltransferase ◽  
Restriction Enzymes ◽  
Dna Binding Protein ◽  
E Coli ◽  
Dna Binding Affinity ◽  
Higher Eukaryotes

AbstractTargeted DNA methylation is a technique that aims to methylate cytosines in selected genomic loci. In the most widely used approach a CG-specific DNA methyltransferase (MTase) is fused to a sequence specific DNA binding protein, which binds in the vicinity of the targeted CG site(s). Although the technique has high potential for studying the role of DNA methylation in higher eukaryotes, its usefulness is hampered by insufficient methylation specificity. One of the approaches proposed to suppress methylation at unwanted sites is to use MTase variants with reduced DNA binding affinity. In this work we investigated how methylation specificity of chimeric MTases containing variants of the CG-specific prokaryotic MTase M.SssI fused to zinc finger or dCas9 targeting domains is influenced by mutations affecting catalytic activity and/or DNA binding affinity of the MTase domain. Specificity of targeted DNA methylation was assayed in E. coli harboring a plasmid with the target site. Digestions of the isolated plasmids with methylation sensitive restriction enzymes revealed that specificity of targeted DNA methylation was dependent on the activity but not on the DNA binding affinity of the MTase. These results have implications for the design of strategies of targeted DNA methylation.

Two Indo-Portuguese Creoles in contrast

2002 ◽  
Vol 17 (2) ◽  
pp. 191-236 ◽  
Author(s):  
J. Clancy Clements ◽  
Andrew J. Koontz-Garboden
Keyword(s):  
Comparative Study ◽  
Independent Origin ◽  
The Common ◽  
Lexical Items ◽  
Syntactic Properties

This paper presents a comparative study of two Indo-Portuguese creoles, Korlai Creole Portuguese (KP) and Daman Creole Portuguese (DP). Using recently collected data, the phonology, pronominal systems, TMA markers, syntactic properties, and lexical items of KP and DP are compared and contrasted. The question of the common vs. independent origin of KP and DP is also discussed.

A comparative study of the ?utriculo-endolymphatic valve? in some of the common mammals

1930 ◽  
Vol 46 (4) ◽  
pp. 333-347 ◽  
Author(s):  
Erwin Frederick Hoffman ◽  
Theodore Hieronymus Bast
Keyword(s):  
Comparative Study ◽  
The Common

scholarly journals Comparative Study of Ag Nanostructures: Molecular Simulations, Electrochemical Behavior, and Antibacterial Effect

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Álvaro de Jesús Ruíz-Baltazar ◽  
Simón Yobanny Reyes-López ◽  
D. Larrañaga ◽  
R. Pérez
Keyword(s):  
Cyclic Voltammetry ◽  
Ethylene Glycol ◽  
Comparative Study ◽  
Sodium Borohydride ◽  
Electrochemical Behavior ◽  
Antibacterial Effect ◽  
Chemical Reduction ◽  
Molecular Simulations ◽  
The Other ◽  
E Coli

Nanoparticles of Ag with different sizes and structures were obtained and studied. Two methods for reductions of Ag ions were employed, chemical reduction by sodium borohydride and ethylene glycol. Cuboctahedral and icosahedral structures were obtained. Molecular simulations were carried out in order to evaluate the reactivity of both structures. On the other hand, the electrochemical activity and antibacterial effect (E. coli) of the cuboctahedral and icosahedral structures were measured experimentally. The results obtained by molecular simulation, cyclic voltammetry, and antibacterial effect were compared and discussed in this work.

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