scholarly journals Cyto/Biocompatibility of Dopamine Combined with the Antioxidant Grape Seed-Derived Polyphenol Compounds in Solid Lipid Nanoparticles

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 916
Author(s):  
Adriana Trapani ◽  
Lorenzo Guerra ◽  
Filomena Corbo ◽  
Stefano Castellani ◽  
Enrico Sanna ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Chemical Characterization ◽  
Grape Seed ◽  
Lipid Nanoparticles ◽  
Non Invasive ◽  
Solid Lipid ◽  
Physico Chemical ◽  
Release Studies ◽  
Nigrostriatal Neurons ◽  
6 Hydroxydopamine

Background: The loss of nigrostriatal neurons containing dopamine (DA) together with the “mitochondrial dysfunction” in midbrain represent the two main causes related to the symptoms of Parkinson’s disease (PD). Hence, the aim of this investigation is to co-administer the missing DA and the antioxidant grape seed-derived proanthocyanidins (grape seed extract, GSE) in order to increase the levels of the neurotransmitter (which is unable to cross the Blood Brain Barrier) and reducing the oxidative stress (OS) related to PD, respectively. Methods: For this purpose, we chose Solid Lipid Nanoparticles (SLN), because they have been already proven to increase DA uptake in the brain. DA-SLN adsorbing GSE (GSE/DA-SLN) were formulated and subjected to physico-chemical characterization, and their cytocompatibility and protection against OS were examined. Results: GSE was found on SLN surface and release studies evidenced the efficiency of GSE in preventing DA autoxidation. Furthermore, SLN showed high mucoadhesive strength and were found not cytotoxic to both primary Olfactory Ensheathing and neuroblastoma SH-SY5Y cells by MTT test. Co-administration of GSE/DA-SLN and the OS-inducing neurotoxin 6-hydroxydopamine (100 μM) resulted in an increase of SH-SY5Y cell viability. Conclusions: Hence, SLN formulations containing DA and GSE may constitute interesting candidates for non-invasive nose-to-brain delivery.

scholarly journals Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 344
Author(s):  
Adriana Trapani ◽  
María Ángeles Esteban ◽  
Francesca Curci ◽  
Daniela Erminia Manno ◽  
Antonio Serra ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Antioxidant Properties ◽  
Grape Seed ◽  
Lipid Nanoparticles ◽  
Antioxidant Compounds ◽  
Gilthead Seabream ◽  
Antioxidant Genes ◽  
Solid Lipid ◽  
Antioxidant Agents ◽  
Fish Cells

The supply of nutrients, such as antioxidant agents, to fish cells still represents a challenge in aquaculture. In this context, we investigated solid lipid nanoparticles (SLN) composed of a combination of Gelucire® 50/13 and Precirol® ATO5 to administer a grape seed extract (GSE) mixture containing several antioxidant compounds. The combination of the two lipids for the SLN formation resulted in colloids exhibiting mean particle sizes in the range 139–283 nm and zeta potential values in the range +25.6–43.4 mV. Raman spectra and X-ray diffraction evidenced structural differences between the free GSE and GSE-loaded SLN, leading to the conclusion that GSE alters the structure of the lipid nanocarriers. From a biological viewpoint, cell lines from gilthead seabream and European sea bass were exposed to different concentrations of GSE-SLN for 24 h. In general, at appropriate concentrations, GSE-SLN increased the viability of the fish cells. Furthermore, regarding the gene expression in those cells, the expression of antioxidant genes was upregulated, whereas the expression of hsp70 and other genes related to the cytoskeleton was downregulated. Hence, an SLN formulation containing Gelucire® 50/13/Precirol® ATO5 and GSE may represent a compelling platform for improving the viability and antioxidant properties of fish cells.

Synthesis and Characterization of Valacyclovir HCl Hybrid Solid Lipid Nanoparticles by Using Natural Oils

2019 ◽  
Vol 13 (1) ◽  
pp. 46-61
Author(s):  
Archana Chacko ◽  
Amaldoss M.J. Newton
Keyword(s):  
Zeta Potential ◽  
Solid Lipid Nanoparticles ◽  
Grape Seed ◽  
Lipid Nanoparticles ◽  
Correlation Spectroscopy ◽  
Good Stability ◽  
Hybrid Nanoparticles ◽  
Solid Lipid ◽  
Natural Oils ◽  
Grape Seed Oil

Background: The Jojoba Simmondsia Chinensis oil is used as one of the main ingredients which has an antioxidant, moisturizing and stabilizing activity. Likewise, grape seed (Vitis vinifera) oil is also used in this preparation which also has some remarkable medicinal properties such as antioxidant, astringent and is also used as a moisturizer. The Valacyclovir Solid Lipid Nanoparticles (SLN) are prepared in combination. Objective: The prime objective of the study was to prepare a nanodispersion with good stability indicating zeta potential. The formulations were prepared by varying concentrations of jojoba oil and grape seed oil which form the hybrid nanoparticles with the drug. Methods: The high-pressure hot-homogenization technique was used to prepare the nanoparticles. The prepared nanoparticles were subjected to characterization analysis such as Mean particle size, Zaverage, and Zeta potential by using Dynamic Light Scattering (DLS) and Photon Correlation Spectroscopy (PCS). The best formulation was subjected to Transmission Electron Microscopy (TEM) technique for surface morphology and other characterizations. The crystalline pattern of the drug alone, drug-loaded nanoparticles and nanoparticles without the drug was studied by XRD. The drug excipients compatibility studies were performed by using Fourier-Transform Infrared Spectroscopy (FTIR) Differential Scanning Calorimetry and (DSC). The other factors such as in vitro drug release, and % drug entrapment efficiency were studied by using suitable methods. Results: The results demonstrated that the particles are in nano range with good stability with appreciable Zeta potential (-48.2±mV). The selected formulations were analyzed for MPS which demonstrated the value of 306.7±183.4 and 416.5±289.3. The best formulation VNP5 demonstrated the Bellshaped curve and confirmed the uniform distribution. Conclusion: Based on the patents, it was demonstrated that valacyclovir is widely used in the treatment and prophylaxis of viral infections in human, particularly infections caused by the herpes group of viruses. Valacyclovir is an effective drug for the treatment of cold sores.

scholarly journals Correction: Synthetic ultra-long chain fatty acyl based amphiphilic lipids as a dual function excipient for the production of surfactant-free solid lipid nanoparticles (SF-SLNs): a physico-chemical study

2016 ◽  
Vol 18 (15) ◽  
pp. 4312-4312
Author(s):  
Wei Wei ◽  
Xiaonan Lu ◽  
Zegao Wang ◽  
Mingdong Dong ◽  
Fengqin Feng ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Chemical Study ◽  
Lipid Nanoparticles ◽  
Fatty Acyl ◽  
Dual Function ◽  
Solid Lipid ◽  
Physico Chemical ◽  
Long Chain

Correction for ‘Synthetic ultra-long chain fatty acyl based amphiphilic lipids as a dual function excipient for the production of surfactant-free solid lipid nanoparticles (SF-SLNs): a physico-chemical study’ by Wei Wei et al., Green Chem., 2016, DOI: 10.1039/c6gc00866f.

Synthetic ultra-long chain fatty acyl based amphiphilic lipids as a dual function excipient for the production of surfactant-free solid lipid nanoparticles (SF-SLNs): a physico-chemical study

2016 ◽  
Vol 18 (14) ◽  
pp. 3962-3971 ◽  
Author(s):  
Wei Wei ◽  
Xiaonan Lu ◽  
Zegao Wang ◽  
Mingdong Dong ◽  
Fengqin Feng ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Chemical Study ◽  
Lipid Nanoparticles ◽  
Fatty Acyl ◽  
Dual Function ◽  
Solid Lipid ◽  
Physico Chemical ◽  
Long Chain

Behenoyl (22 : 0) based amphiphilic lipids are synthesized, some of which demonstrate excellent dual functionality as both solid excipients and emulsifiers in producing uniform solid lipid nanoparticles (SLNs).

scholarly journals Preparation, In Vitro characterization and stability studies of ropinirole lipid nanoparticles enriched hydrogel for treatment of neurodegeneration diseases

2021 ◽  
Vol 11 (2-S) ◽  
pp. 66-75
Author(s):  
Kumara Swamy Samanthula ◽  
Ramesh Alli ◽  
Thirupathi Gorre
Keyword(s):  
Particle Size ◽  
Solid Lipid Nanoparticles ◽  
In Vitro Release ◽  
Entrapment Efficiency ◽  
Lipid Nanoparticles ◽  
Nanostructured Lipid Carriers ◽  
Solid Lipid ◽  
Release Studies ◽  
Vitro Release

Ropinirole (RP), is a selective dopamine agonist that is used alone or with other medications to treat the symptoms of Parkinson’s disease (PD). RP has low bioavailability of only about 50% due to the first-pass metabolism, and it requires frequent dosing during oral administration. The objective of the current research was to develop RP loaded solid lipid nanoparticles (RP-SLNs), nanostructured lipid carriers (RP-NLCs), and their corresponding hydrogels (RP-SLN-C and RP-NLC-C) that might improve efficacy in PD treatment. RP nanoparticles were prepared by homogenization aided probe sonication method and optimized based on particle size, polydispersity index (PDI), zeta potential (ZP), assay, entrapment efficiency, and in vitro release studies. Optimized formulations were converted to hydrogel formulations using Carbopol 934 as a gelling polymer and optimized based on rheological and release characteristics. Optimized formulations were further evaluated using differential scanning calorimetry (DSC), powder X-ray diffractometry (PXRD), scanning electron microscopy (SEM), freeze-drying, and stability study at refrigerated and room temperatures. The optimized RP-SLN formulation showed particle size and entrapment efficiency of 213.5±3.8 nm and 77.9±3.1% compared to 190.6±3.7 nm and 85.7±1.7% for optimized RP-NLC formulation. PXRD supplemented and confirmed DSC results, RP was entrapped in a molecularly dispersed state inside the core of the lipid nanocarrier. Furthermore, RP loaded lipid nanocarriers revealed a spherical shape in SEM images. In vitro release studies demonstrated sustained release profiles for RP from SLNs, NLCs, and their hydrogels over 24 h and were stable over three months at 4ºC and 25ºC storage conditions. Keywords: Parkinson’s disease, Ropinirole, Solid lipid nanoparticles, Nanostructured lipid carriers, Hydrogel.

scholarly journals Physical-Chemical Characterization and Formulation Considerations for Solid Lipid Nanoparticles

2015 ◽  
Vol 17 (3) ◽  
pp. 640-651 ◽  
Author(s):  
Harsh Chauhan ◽  
Sarat Mohapatra ◽  
Daniel J. Munt ◽  
Shantanu Chandratre ◽  
Alekha Dash
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Chemical Characterization ◽  
Lipid Nanoparticles ◽  
Solid Lipid ◽  
Physical Chemical

scholarly journals Formulation and Evaluation of Fluvastatin Loaded Solid Lipid Nanoparticles

2021 ◽  
Vol 69 (1) ◽  
Author(s):  
DR. P. Tripura Sundari ◽  
Akhila G.
Keyword(s):  
Drug Release ◽  
Solid Lipid Nanoparticles ◽  
Entrapment Efficiency ◽  
Tween 80 ◽  
Lipid Nanoparticles ◽  
Solid Lipid ◽  
Drug Content ◽  
Peg 400 ◽  
Bcs Class Ii ◽  
Release Studies

In the present work Fluvastatin was taken which is anti-hyper lipidemic drug used for chronic treatment. It belongs to BCS class-II which means low soluble & high permeable drug. The current dosage regimen is 20-80mg for 2-3 times a day. The present work aims at reducing the dose of the drug by converting it into nanocarrier systems. For this purpose, SLN’s were prepared by employing Compritol as lipid, Soyalecithin as lipophilic surfactant, Tween-80/PEG-400 as hydrophilic surfactant systems. The prepared formulations were named as T1 to T4 with Tween-80 and P1 to P4 with PEG-400. All the prepared formulations are evaluated for drug content, entrapment efficiency and drug release studies. Among all the preparations best formulation was found to be P3 in terms of Drug content of 96.2%, Entrapment efficiency of 90.87% , Drug release of 61.0%, Particle size of 578nm with Zeta potential of -26mV. The present study conclusively demonstrated that the solubility of drug was improved by entrapment of drug into solid lipid carrier which led to prolongation of drug release.

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