scholarly journals Comparison of Multiple Bioactive Constituents in the Corolla and Other Parts of Abelmoschus manihot

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1864
Author(s):  
Shengxin Yin ◽  
Yuqi Mei ◽  
Lifang Wei ◽  
Lisi Zou ◽  
Zhichen Cai ◽  
...  
Keyword(s):  
Ion Trap ◽  
Distribution Patterns ◽  
Scientific Data ◽  
The Other ◽  
Bioactive Constituents ◽  
Medicinal Value ◽  
Components Analysis ◽  
Fast Performance Liquid Chromatography ◽  
Different Parts

Abelmoschus manihot (L.) Medic (AM), called Huangshukui in Chinese, is a widely used medicinal plant. Each part of AM has medicinal value, including Abelmoschi Radix (AR), Abelmoschi Herba (AH), Abelmoschi Folium (AF), Abelmoschi Corolla (AC), and Abelmoschi Semen (AS). However, only AC is documented in the Chinese Pharmacopoeia. In order to investigate whether there is any difference between AC and the other parts of AM, an analytical method based on ultra-fast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) was established for the simultaneous determination of 35 constituents in different parts of AM. Moreover, principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were applied to classify and evaluate the different parts of AM based on the content of the 35 constituents. The total contents of the 35 constituents in AC were significantly higher than in the other parts of AM and the results revealed significant differences between AC and the other parts of AM. Eight constituents were remarkably related to the sample classifications. This research does not just provide the basic information for revealing the distribution patterns in different parts of AM from the same origin, but also complements some of the scientific data for the comprehensive quality evaluation of AC.

scholarly journals Quality Evaluation of Ophiopogonis Radix from Two Different Producing Areas

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3220 ◽  
Author(s):  
Mengxia Tan ◽  
Jiali Chen ◽  
Chengcheng Wang ◽  
Lisi Zou ◽  
Shuyu Chen ◽  
...  
Keyword(s):  
Ion Trap ◽  
Comprehensive Evaluation ◽  
Functional Health ◽  
Health Food ◽  
Bioactive Constituents ◽  
Components Analysis ◽  
Grey Relational ◽  
Insight Into

Ophiopogonis Radix, also known as Mai-dong in Chinese, was a commonly used traditional Chinese medicine (TCM) and functional health food. Two products of Ophiopogonis Radix are largely produced in the Sichuan and Zhejiang province, which are called “Chuan maidong (CMD)” and “Zhe maidong (ZMD)” respectively. To distinguish and evaluate the quality of CMD and ZMD, an analytical method based on ultra-fast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) was established for simultaneous determination of 32 constituents including 4 steroidal saponins, 3 homisoflavonoids, 15 amino acids, and 10 nucleosides in 27 Mai-dong samples from Sichuan and Zhejiang. Furthermore, principal components analysis (PCA), partial least squares discriminant analysis (PLS-DA), t-test, and grey relational analysis (GRA) were applied to discriminate and evaluate the samples from Sichuan and Zhejiang based on the contents of 32 constituents. The results demonstrated that the bioactive constituents in CMD and ZMD were significantly different, and CMD performed better in the quality assessment than ZMD. This study not only provides a basic information for differentiating CMD and ZMD, but offers a new insight into comprehensive evaluation and quality control of Ophiopogonis Radix from two different producing areas.

A rapid and highly sensitive method for simultaneous determination of bioactive constituents in leaf extracts of six Ocimum species using ultra high performance liquid chromatography-hybrid linear ion trap triple quadrupole mass spectrometry

2016 ◽  
Vol 8 (2) ◽  
pp. 333-341 ◽  
Author(s):  
Renu Pandey ◽  
Preeti Chandra ◽  
Brijesh Kumar ◽  
Bhupender Dutt ◽  
Kulwant Rai Sharma
Keyword(s):  
Food Processing ◽  
High Performance ◽  
Ion Trap ◽  
Linear Ion Trap ◽  
Quadrupole Mass ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Highly Sensitive ◽  
Ocimum Species

Ocimum species have tremendous value in pharmaceutical, perfumery, food processing and cosmetic industries, also in traditional rituals and medicines.

scholarly journals Distribution Patterns for Bioactive Constituents in Pericarp, Stalk and Seed of Forsythiae Fructus

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 340 ◽  
Author(s):  
Lifang Wei ◽  
Yuqi Mei ◽  
Lisi Zou ◽  
Jiali Chen ◽  
Mengxia Tan ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Folk Medicine ◽  
Plant Secondary Metabolites ◽  
Distribution Patterns ◽  
Scientific Data ◽  
Bioactive Constituents ◽  
Phenylethanoid Glycosides ◽  
Liquid Chromatography Tandem Mass ◽  
Phytochemical Profiles ◽  
One Way Anova

Forsythiae Fructus (FF) is a widely used folk medicine in China, Japan, and Korea. The distribution of bioactive constituents throughout the fruit segments has rarely been addressed, although mounting evidence suggests that plant secondary metabolites are synthesized and distributed regularly. The phytochemical profiles of three segments of FF (pericarp, stalk and seed) were firstly revealed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based quantitative analysis of twenty-one bioactive constituents, including three phenylethanoid glycosides, five lignans, eight flavonoids, and five phenolic acids to explore the spatial distribution of bioactive constituents. Furthermore, the hierarchical clustering analysis (HCA) and one-way analysis of variance (one-way ANOVA) were conducted to visualize and verify the distribution regularity of twenty-one analytes among three segments. The results showed that phytochemical profiles of the three segments were similar, i.e., phenylethanoid glycosides covering the most part were the predominant compounds, followed by lignans, flavonoids and phenolic acids. Nevertheless, the abundance of twenty-one bioactive constituents among three segments was different. Specifically, phenylethanoid glycosides were highly expressed in the seed; lignans were primarily enriched in the stalk; flavonoids were largely concentrated in the pericarp, while the contents of phenolic acids showed no much difference among various segments. The research improves our understanding of distribution patterns for bioactive constituents in FF, and also complements some scientific data for further exploring the quality formation mechanism of FF.

scholarly journals Spectrophotometric Determination of Caramel Content in Spirits Aged in Oak Casks

2002 ◽  
Vol 85 (3) ◽  
pp. 744-750 ◽  
Author(s):  
Mauricio Boscolo ◽  
Luiz G Andrade-Sobrinho ◽  
Benedito S Lima-Neto ◽  
Douglas W Franco ◽  
Marcia Miguel Castro Ferreira
Keyword(s):  
Clustering Analysis ◽  
Geographic Origin ◽  
The Other ◽  
Ratio Method ◽  
Calibration Model ◽  
First Derivative ◽  
The Mean ◽  
Components Analysis ◽  
Clear Differentiation

Abstract A new methodology was developed for determination of caramel in spirits aged in oak casks. The method is based on differences between the electronic spectra of oak aqueous alcoholic extracts and caramel solutions in the same solvent. The data were treated by 2 different approaches: the simplest one was based on the plot of caramel concentration versus the ratio of absorbance at 210 and 282 nm; the other was based on a partial least squares (PLS) calibration model using the first derivative of the spectral data. Both methodologies were applied to analysis of 159 aged spirit samples. The mean caramel content of several Brazilian sugar cane spirits (cachaça) and all United States whiskies was smaller than that of Scottish whiskies and other brandies from several countries. Correlation was good between caramel concentrations for the same sample calculated by the 2 methods. The uncertainties following PLS and the absorbance ratio method were 0.01 and 0.03 g/L, respectively, for a sample containing 0.45 g/L caramel. Treatment of UV-VIS spectra by pattern recognition using hierarchical clustering analysis and principal components analysis allowed discrimination of the samples as a function of their caramel content. It was possible to distinguish U.S. whiskies from other whiskies, but a clear differentiation among Brazilian cachaças as a function of their geographic origin was not feasible. Small caramel quantities as low as 0.08 g/L were clearly detected by these methodologies.

scholarly journals Identification of Poinsettia Cultivars Using RAPD Markers

HortScience ◽  
1997 ◽  
Vol 32 (1) ◽  
pp. 122-124 ◽  
Author(s):  
Jing-Tian Ling ◽  
Roger Sauve ◽  
Nick Gawel
Keyword(s):  
Cluster Analysis ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
The Other ◽  
Euphorbia Pulcherrima ◽  
Randomly Amplified Polymorphic Dna ◽  
Upgma Cluster Analysis ◽  
Leaf Tissues ◽  
Components Analysis

Randomly amplified polymorphic DNA (RAPD) techniques were used to compare the DNA from leaf tissues of nine commercial poinsettia (Euphorbia pulcherrima Wild ex Klotzsch) cultivars. Amplification occurred in 57 out of 60 (95%) tested primers. Nine primers that revealed polymorphisms among cultivars were selected for further evaluation. Forty-eight RAPD bands were scored from these primers, and 33 (69%) were polymorphic. All tested cultivars could be discriminated with seven bands generated from primers OPB7 and OPC13. Results of a UPGMA cluster analysis and principal components analysis placed the nine cultivars into two groups: one group consisted of `Jingle Bells', `Supjibi', and `V-17 Angelika', the other of `V-14 Glory', `Red Sails', `Jolly Red', and `Freedom'. `Lilo Red' and `Pink Peppermint' belonged to the latter group, but were relatively distant from other cultivars in that group. These results indicate that RAPDs are efficient for identification of poinsettia cultivars and for determination of the genetic relationships among cultivars.

scholarly journals Development of a Gas Chromatographic/Mass Spectrometric Method for Determination of Phthalates in Oily Foods

2010 ◽  
Vol 93 (1) ◽  
pp. 315-322 ◽  
Author(s):  
Anna Sannino
Keyword(s):  
Ion Trap ◽  
Mass Spectrometric ◽  
The Other ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Florisil Column ◽  
Dibutyl Sebacate ◽  
Full Scan ◽  
Chromatographic Mass

Abstract A GC/MS method was developed for simultaneous determination of 12 phthalates and four other plasticizersacetyl tributyl citrate, di(2-ethylhexyl) adipate, dibutyl sebacate, di-isononyl cyclohexane 1,2-dicarboxylate (DINCH)in vegetable oil, pesto, and tomato sauce. Samples were extracted with acetonitrile and cleaned on a Florisil column. The final extract was analyzed by GC in combination with ion trap MS. The phthalates and di-isononyl cyclohexane 1,2-dicarboxylate were detected by MS/MS, while the other three plasticizers were monitored in the same GC run using full scan mode. The analytical process was validated in each matrix by the analysis of blank samples. Performance characteristics, such as linearity, LOQ, precision, and recoveries were studied. Studies at fortification levels of 0.25200 mg/kg gave mean recoveries ranging from 71 to 106 and RSD values between 7 and 12 for all compounds. LOQs were 0.0500.10 mg/kg for all the target compounds except di-isononyl phthalate, di-isodecyl phthalate, and DINCH (2.0 mg/kg).

scholarly journals Wasp Venom Biochemical Components and Their Potential in Biological Applications and Nanotechnological Interventions

Toxins ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 206
Author(s):  
Aida Abd El-Wahed ◽  
Nermeen Yosri ◽  
Hanem H. Sakr ◽  
Ming Du ◽  
Ahmed F. M. Algethami ◽  
...  
Keyword(s):  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Biological Applications ◽  
Bioactive Constituents ◽  
Wasp Venom ◽  
New Agents ◽  
Current Review ◽  
Medicinal Value ◽  
Antigen 5 ◽  
Different Parts

Wasps, members of the order Hymenoptera, are distributed in different parts of the world, including Brazil, Thailand, Japan, Korea, and Argentina. The lifestyles of the wasps are solitary and social. Social wasps use venom as a defensive measure to protect their colonies, whereas solitary wasps use their venom to capture prey. Chemically, wasp venom possesses a wide variety of enzymes, proteins, peptides, volatile compounds, and bioactive constituents, which include phospholipase A2, antigen 5, mastoparan, and decoralin. The bioactive constituents have anticancer, antimicrobial, and anti-inflammatory effects. However, the limited quantities of wasp venom and the scarcity of advanced strategies for the synthesis of wasp venom’s bioactive compounds remain a challenge facing the effective usage of wasp venom. Solid-phase peptide synthesis is currently used to prepare wasp venom peptides and their analogs such as mastoparan, anoplin, decoralin, polybia-CP, and polydim-I. The goal of the current review is to highlight the medicinal value of the wasp venom compounds, as well as limitations and possibilities. Wasp venom could be a potential and novel natural source to develop innovative pharmaceuticals and new agents for drug discovery.

scholarly journals Phytochemical screening and analysis of heavy metals of Nerium oleander (L.) leaves

2020 ◽  
Vol 10 (4) ◽  
pp. 346
Author(s):  
Jamila Fliou ◽  
Ouassima Riffi ◽  
Ali Amechrouq ◽  
Mohammed Elhourri ◽  
Mostafa El Idrissi ◽  
...  
Keyword(s):  
Absorption Spectrometry ◽  
Scientific Data ◽  
The Other ◽  
Nerium Oleander ◽  
Phytochemical Screening ◽  
Low Concentrations ◽  
Anthracene Derivatives ◽  
Harmful Effects ◽  
Pb Concentration

<p>The main objective of this research is studying phytochemical screening of the extracts of polluted <em>Nerium oleander</em> (L.), and unpolluted<em> Nerium oleander</em> (L.) collected from Meknes region (Morocco), and determination of metals concentration that can have harmful effects on human health. In addition, the study provides scientific data.</p><p>Studies have shown that the two extracts of polluted <em>Nerium oleander</em> (L.) and unpolluted <em>Nerium oleander</em> (L.) plant are rich in flavonoids, catechic and gallic tannins. In contrast, anthracene derivatives and flavonoids are absent. On the other hand, the comparative analysis of the results of atomic absorption spectrometry showed that the two plants of polluted <em>Nerium oleander</em> (L.) and unpolluted <em>Nerium oleander</em> (L.) contain a fairly high content of Na, Ca and Mg while the Pb concentration has exceeded the standard given by the WHO. While lithium and iron are present in low concentrations.</p>

scholarly journals A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 167
Author(s):  
Yuqi Mei ◽  
Lifang Wei ◽  
Chuan Chai ◽  
Lisi Zou ◽  
Xunhong Liu ◽  
...  
Keyword(s):  
Discriminant Analysis ◽  
Chinese Medicine ◽  
Protocatechuic Acid ◽  
Blood Stasis ◽  
Distribution Patterns ◽  
Blood Stasis Syndrome ◽  
Heat Map ◽  
Components Analysis ◽  
Spatholobus Suberectus

Spatholobi Caulis (SC), the vine stem of Spatholobus suberectus Dunn, is a widely used traditional Chinese medicine (TCM) for the treatment of blood stasis syndrome and related diseases. Xylem and phloem are the main structures of SC and the color of xylem in SC is red brown or brown while the phloem with resin secretions is reddish brown to dark brown. They are alternately arranged in a plurality of concentric or eccentric rings. In order to investigate the distribution patterns of metabolites in xylem and phloem of SC, an analytical method based on UFLC–QTRAP–MS/MS was established for simultaneous determination of 22 constituents including four flavanols, nine isoflavones, two flavonols, two dihydroflavones, one flavanonol, one chalcone, one pterocarpan, one anthocyanidin and one phenolic acid in the samples (xylem and phloem) from Laos. Furthermore, according to the contents of 22 constituents, heat map, principal components analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS–DA) and t–test were used to evaluate the samples and discover the differences between xylem and phloem of SC. The results indicated that the measured ingredients in xylem and phloem were significantly different. To be specific, the contents of flavonoids in xylem were higher than that in phloem, while the content of protocatechuic acid showed a contrary tendency. This study will not only reveal the distribution patterns of metabolites in xylem and phloem of SC but also facilitate further study on their quality formation.

scholarly journals Distribution Patterns of Vector Snails and Trematode Cercaria in their Vectors in Some Selected Areas of Mymensingh

2013 ◽  
Vol 5 (2) ◽  
pp. 37-46 ◽  
Author(s):  
Z Islam ◽  
MZ Alam ◽  
S Akter ◽  
BC Roy ◽  
MMH Mondal
Keyword(s):  
Natural Resources ◽  
Distribution Patterns ◽  
Coastal Areas ◽  
The Other ◽  
Snail Species ◽  
Population Densities ◽  
Larval Stages ◽  
Indoplanorbis Exustus ◽  
Mixed Types

Different species of vector snails carry larval stages of different trematodes. Studies were performed to investigate different snail species and parthinate of trematodes carried by them, in some selected areas of Mymensingh Sadar, Mymensingh. Snails were collected by hand picking method and examined after crushing. Among 864 tested snails, it revealed the presence of six species of snails like Lymnaea auricularia 145 (16.8%), Lymnaea luteola 205 (23.7%), Indoplanorbis exustus 273 (31.6%), Vivipara spp. 164 (18.9%), Brotia spp. 35 (4.1%) and Pila spp. 42 (4.8%) in the study areas. Among them 5.8% L. luteola and 6.2% L. auricularia were infected with gymnacephalus cercariae. Again, 1.4% L. auricularia and 1.8% I. exustus were infected with echinostome cercaria. In this study, 1.8% Vivipara spp. and 2.6% I. exustus were positive for furcocercus cercaria. On the other hand, 1.8% Vivipara spp. was positive for xiphidocercus cercariae and 1.8% I. exustus was also positive for amphistome cercariae. One or more species of snails were found to harbor single or mixed types of cercariae such as gymnacepahlus, echinostome and furcocercous. Population densities of different species of snails were determined in per unit time which were statistically significant (p<0.01). More studies are needed to focus on the determination of different vector snails and cercariae carried by them throughout the Bangladesh especially from plain lands, hilly areas, alluvial lands and coastal areas during four conventional seasons.DOI: http://dx.doi.org/10.3329/jesnr.v5i2.14599 J. Environ. Sci. & Natural Resources, 5(2): 37-46 2012

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