scholarly journals A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 167
Author(s):  
Yuqi Mei ◽  
Lifang Wei ◽  
Chuan Chai ◽  
Lisi Zou ◽  
Xunhong Liu ◽  
...  

Spatholobi Caulis (SC), the vine stem of Spatholobus suberectus Dunn, is a widely used traditional Chinese medicine (TCM) for the treatment of blood stasis syndrome and related diseases. Xylem and phloem are the main structures of SC and the color of xylem in SC is red brown or brown while the phloem with resin secretions is reddish brown to dark brown. They are alternately arranged in a plurality of concentric or eccentric rings. In order to investigate the distribution patterns of metabolites in xylem and phloem of SC, an analytical method based on UFLC–QTRAP–MS/MS was established for simultaneous determination of 22 constituents including four flavanols, nine isoflavones, two flavonols, two dihydroflavones, one flavanonol, one chalcone, one pterocarpan, one anthocyanidin and one phenolic acid in the samples (xylem and phloem) from Laos. Furthermore, according to the contents of 22 constituents, heat map, principal components analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS–DA) and t–test were used to evaluate the samples and discover the differences between xylem and phloem of SC. The results indicated that the measured ingredients in xylem and phloem were significantly different. To be specific, the contents of flavonoids in xylem were higher than that in phloem, while the content of protocatechuic acid showed a contrary tendency. This study will not only reveal the distribution patterns of metabolites in xylem and phloem of SC but also facilitate further study on their quality formation.

2009 ◽  
Vol 17 (04) ◽  
pp. 531-546 ◽  
Author(s):  
SHUZHEN GUO ◽  
JIANXIN CHEN ◽  
HUIHUI ZHAO ◽  
WEI WANG ◽  
JIANQIANG YI ◽  
...  

Building an animal model for a disease is a better avenue to understand the inner mechanism of it. Traditional Chinese Medicine accumulated much practical experience and a large amount of literature to heal diseases during the past 3000 years. However, as there is no available animal model for TCM research because syndrome, the core of TCM theory, it is hard to be diagnosed from animals. In this paper, we present a novel strategy to build and evaluate an animal model for syndrome in TCM in the context of a disease. We first carried out a clinical epidemiology survey for a syndrome (Blood stasis syndrome, BSS) diagnosed by TCM experts in the context of a disease (Unstable angina, UA). Meanwhile, the blood samples of patients included in the survey were collected and measured as physical and chemical specifications by laboratory examinations. Alternatively, we used supervised data mining methods to build association between the specifications and the syndrome in the context of UA. The accuracy of classification was used to evaluate performance of the association built. Finally, we built an animal model for myocardial ischemia and validated the model by established diagnosis criterion of myocardial ischemia. Furthermore, the built association was used to evaluate whether an animal is with BSS. The results indicated that the strategy successfully evaluates and separates the animal model for syndrome in TCM from the counterpart for myocardial ischemia. The novel strategy presented in the paper provides a better insight to understand the nature of syndrome in TCM and pave a basis for personalized therapies of UA.


RSC Advances ◽  
2017 ◽  
Vol 7 (89) ◽  
pp. 56471-56483
Author(s):  
Jing-Jing Xu ◽  
Feng Xu ◽  
Shu-Jie Shen ◽  
Teng Li ◽  
Yi-Fan Zhang ◽  
...  

Dynamic changes of the metabolic network during the evolution of a syndrome based on the toxic heat and blood stasis syndrome (THBSS) rat model have been elucidated for the first time.


2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ye Huang ◽  
Jing-Shang Wang ◽  
Hui-jun Yin ◽  
Ke-ji Chen

Blood stasis syndrome (BSS), a comprehensive pathological state, is one of the traditional Chinese medicine syndromes of coronary heart disease (CHD). In our previous study, we investigated that FcγRIIIA (also called CD14+CD16+monocyte subpopulation) is one of the differentially expressed genes related to CHD patients and its possible role in the atherosclerotic formation and plaque rupture. However, whether or not the deregulation of CD14+CD16+monocyte subpopulation expression is implicated in the pathogenesis of CHD patients with BSS has not yet been elucidated. In this study, we found that there was no significant difference between CHD patients with BSS and non-BSS in CD14+CD16+monocyte subpopulation at gene level. Moreover, the protein level of CD14+CD16+monocyte subpopulation in CHD patients with BSS was increased significantly when compared to the CHD patients with non-BSS. Additionally, the level of inflammatory cytokines downstream of CD14+CD16+monocyte subpopulation such as TNF-αand IL-1 in sera was much higher in CHD patients with BSS than that in CHD patients with non-BSS. Taken together, these results indicated that CD14+CD16+monocyte subpopulation was implicated in the pathogenesis of CHD patients with BSS, which may be one of the bases of the essence of BSS investigation.


Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1864
Author(s):  
Shengxin Yin ◽  
Yuqi Mei ◽  
Lifang Wei ◽  
Lisi Zou ◽  
Zhichen Cai ◽  
...  

Abelmoschus manihot (L.) Medic (AM), called Huangshukui in Chinese, is a widely used medicinal plant. Each part of AM has medicinal value, including Abelmoschi Radix (AR), Abelmoschi Herba (AH), Abelmoschi Folium (AF), Abelmoschi Corolla (AC), and Abelmoschi Semen (AS). However, only AC is documented in the Chinese Pharmacopoeia. In order to investigate whether there is any difference between AC and the other parts of AM, an analytical method based on ultra-fast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) was established for the simultaneous determination of 35 constituents in different parts of AM. Moreover, principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were applied to classify and evaluate the different parts of AM based on the content of the 35 constituents. The total contents of the 35 constituents in AC were significantly higher than in the other parts of AM and the results revealed significant differences between AC and the other parts of AM. Eight constituents were remarkably related to the sample classifications. This research does not just provide the basic information for revealing the distribution patterns in different parts of AM from the same origin, but also complements some of the scientific data for the comprehensive quality evaluation of AC.


2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Ying Luo ◽  
Yi Ru ◽  
Huaibo Zhao ◽  
Liu Liu ◽  
Seokgyeong Hong ◽  
...  

Background. Psoriasis has been reported as a high-risk factor for quality of life and survival rate in patients with metabolic disorder. However, there is no animal model for studying this disease. This study aimed to establish and evaluate mouse models of psoriasis with blood stasis syndrome (which is a key to psoriasis pathogenesis, according to Chinese Medicine) complicated with metabolic disorders. Method. Forty-five C57BL/6 mice were randomly divided into the blank control (Control), psoriasis (Imiquimod (IMQ)), psoriasis with metabolic disorders (IMQ + streptozotocin (STZ)), psoriasis with blood stasis syndrome (BSS) (IMQ + BSS), and psoriasis with blood stasis syndrome complicated with metabolic disorders (IMQ + STZ + BSS) groups (n = 9 mice/group). Psoriasis lesions were induced using IMQ cream (on both the ears and back, except in the Control group). Mice of the IMQ + BSS group were fed a half-fat, high-sugar diet and stimulated with ice-water swimming every day. Mice of the IMQ + STZ group were fed a half-fat, high-sugar diet and injected with STZ. Mice of the IMQ + STZ + BSS group were subjected to the same treatments as the IMQ + STZ and IMQ + BSS groups. After induction, the mice in each group were observed for vital signs, ear thickness, body weight, and psoriasis area and severity index (PASI) score. The mice were fasted for 12 h before determination of related laboratory serum indexes. Dorsal skin lesions, aortic arch pathology sections, and signal transducer and activator of transcription 3 (STAT3) were examined by H&E staining and immunohistochemistry. Results. Laboratory indexes in the four model groups were significantly different from those in the Control group (p<0.01); indicators of the IMQ + STZ, IMQ + BSS, and IMQ + STZ + BSS groups showed varying degrees of difference from those of the IMQ group. Conclusions. The established mouse models of psoriasis blood stasis syndrome complicated with glucose and lipid metabolism disorders met the clinical and Chinese Medicine characteristics, and thus they could be used as animal models in future studies of psoriasis complicated with glucose and lipid metabolism disorders.


2011 ◽  
Vol 39 (06) ◽  
pp. 1061-1074 ◽  
Author(s):  
Xiao-Xi Zeng ◽  
Zhao-Xiang Bian ◽  
Tai-Xiang Wu ◽  
Shu-Fei Fu ◽  
Eric Ziea ◽  
...  

Many patients with chronic hepatitis B (CHB) seek help from traditional Chinese medicine (TCM). TCM treatment is based on syndrome differentiation. This study aimed to investigate the syndrome distribution in populations of CHB patients. The pre-specific search strategy was set, and 93 studies (20,106 participants) were identified by electronic and hand searches. The methodological quality of included studies was assessed. Data on syndrome distribution and correlations between syndromes and severity of CHB, were extracted and analyzed. Forty-seven syndromes were identified under 24 different syndrome diagnosis systems for CHB. The majority of included studies reported Liver Depression and Spleen Deficiency (LDSD) (52.54% of studies) or Liver-Gallbladder Dampness Heat (LGDH)/Dampness-Heat Obstructing Middle Energizer (DHME) (32.20%) as the major syndromes in CHB patients without cirrhosis. Pooled analysis revealed that LDSD and LGDH/DHME accounted for 61.19% of participants without cirrhosis. In addition, Liver-Kidney Yin Deficiency (LKYinD) (40.99%) and Spleen-Kidney Yang Deficiency (SKYangD) (40.43%) syndromes were common in patients with severe CHB. The percentage of patients with Blood Stasis syndrome increased as the disease progressed to cirrhosis (32.09%). To conclude, LDSD and LGDH/DHME syndromes are found in a significant majority of CHB patients, particularly in the early stages. LKYinD, SKYangD and Blood Stasis dominate in patients at more advanced stages. More epidemiological studies of high methodological quality on syndrome distribution in CHB and standardization of syndrome differentiation for CHB are required to confirm the trends indicated by the studies reviewed here; confirming these trends can increase the efficacy of treatment and give guidance to doctors.


2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Chenhao Zhang ◽  
Chaolian Huang ◽  
Mingming Wang ◽  
Xiaolin Kong ◽  
Guannan Liu ◽  
...  

Objective. To explore the characters of traditional Chinese medicine (TCM) syndromes after percutaneous coronary intervention (PCI) and to provide syndrome study theoretical evidence for TCM differentiation treatment after PCI through retrospective study. Methods. Patients with coronary heart disease (CHD) who underwent PCI in Cardiovascular Intervention Center of Wangjing Hospital during Dec. 2012 to Dec. 2014 and met the inclusion criteria were enrolled. Retrospective study was then conducted based on patients’ clinical document and angiography data to explore the distribution pattern of TCM syndromes. Results. 801 patients were recruited in the study. TCM syndromes in descending order of their incidence were Qi deficiency and blood stasis syndrome, heart blood stasis syndrome, Qi and Yin deficiency syndrome, phlegm and blood stasis syndrome, Qi stagnation and blood stasis syndrome, Yang asthenia syndrome, heart and kidney yin deficiency syndrome to cold congeal, and blood stasis syndrome in a more to less order. Qi deficiency and blood stasis syndrome was in the most (occurring in 298 patients, 37.20%); Qi and Yin deficiency syndrome occurred in 163 patients (20.35%); heart blood stasis syndrome was shown in 126 patients (15.73%); phlegm and blood stasis syndrome was shown in 95 patients (11.86%). Conclusion. Qi deficiency and blood stasis syndrome was closely associated with post-PCI bleeding, implying that this syndrome might serve as a powerful predictor of GI bleeding as well as a potential supplement to the current predicting and scoring system of bleeding such as CRUSADE.


2020 ◽  
Author(s):  
Min Su ◽  
Gang Cao ◽  
Xiaoli Wang ◽  
Raftery Daniel ◽  
Yan Hong ◽  
...  

Abstract Backgroud: According to the theory of traditional Chinese medicine (TCM), blood stasis syndrome (BSS) is the basis for many cardiovascular diseases. Ginger is often used as Chinese medicine (herb medicine) in China and Southeast Asia for the treatment of cardiovascular, respiratory, and digestive diseases. but the specific mechanism is unclear.Methods: After establishing an acute rat model of blood stasis syndrome, blood and urine of 5 groups of rats were collected for analysis of efficacy indicators. Aortic vessels are used for pathological testing. Serum and urine were used for metabolomics, multivariate statistical analysis was used to explore metabolites and metabolic pathways, and the correlation between metabolites and pharmacodynamic indicators was further explored.Results: In this experiment, the model of blood stasis syndrome was successfully established based on changes in vascular disease and the efficacy index. The experimental results show that the efficacy indicators of dried ginger (DG) extracts of different doses have different degrees of changes than model group (MG), and the high dose of dried ginger group (GJH) changes are the most significant (p < 0.05 or p < 0.01). And 22 metabolites (10 in serum and 12 in urine) were identified and contributed to the blood stasis progress. These metabolites mainly involve seven metabolism pathways in different impact-value. Dried ginger has therapeutic effects on BSS rats by regulating multiple metabolic pathways.Conclusion: This study provides an effective method for understanding the metabolic mechanism of dried ginger extracts on BSS.


Author(s):  
Weibo LU

LANGUAGE NOTE | Document text in Chinese; abstract also in English.中西醫結合包含中醫和西醫之間的團結合作,但更重要的是中醫學和西醫學之間的互相結合。在世界上對傳統醫學曾有過三種方針,即“否定”,“容忍”和“平行”的方針,均不能充分發揮傳統醫學的作用和潛力。中國採取“結合”的方針,較好地解決了這一問題,不僅滿足當前醫療工作的需要,更重要的是在科學研究中,強調創新,兩種醫學的觀點、方法、優勢互補,獲得大量新的研究成果,如針刺原理和針麻研究,青萬素的發現,瘀血證研究等。雖然現代醫學是主流醫學,但尚不足以解決所有問題,如慢性病,老年病等。用結合的思想可以更好地解決各種難題,使醫學科學更快地向前發展,造福於人民。What attitude should we take toward traditional medicine? There have been three types of policies in this regard all over the world. First, the excluding policy prohibits practicing any traditional medicine. Traditional physicians are not qualified to possess the title of physician, and their practice is illegal. Second, the tolerating policy does not make illegal traditional medical practice, but it does not formally affirm the practice. Third, the paralleling policy allows both traditional and modem medical practices, but their use may not overlap. For example, traditional physicians may not use modem medical facilities.Since the establishment of the People’s Republic of China in 1949, a unique policy of integration has been adopted in China. This policy assumes that both traditional Chinese medicine and modern Western medicine have strengths and weaknesses. It requires that both types of medicine be integrated so as to develop more effective methods in treating diseases and preserving health.There are two senses of the integration of traditional Chinese and modern Western medicine. The first sense refers to the integration of the two types of the physicians, i.e., the cooperation between traditional Chinese practitioners and modern Western medical professionals. The second sense refers to the integration of the two disciplines; i.e., to use both traditional Chinese and modern Western medical theories and practices to create new forms of diagnostic and therapeutic approaches and means. The hope was to have the perspectives, methods, and solutions of both types of medicine complement each other.Under the integrating policy, traditional Chinese medicine has been developed tremendously in mainland China. 30 traditional Chinese medical colleges, 2457 traditional Chinese medical hospitals, and 170 traditional Chinese medical research institutions have been established. A number of well-known medical achievements, such as acupunctural analgesia and anesthesia, the discovery of a new type of anti-malaria drug - Qinghaosu (artemisinin), and the study of blood stasis syndrome, have been made by following the integrating strategy.With the rapid growth and development of the diagnostic and therapeutic technologies in modern Western medicine, some individuals are doubtful of the prospect of traditional Chinese medicine and of the necessity of China’s integrating strategy. However, no matter how advanced modern Western medicine as a form of medicine has become, it will not be able to handle all diseases or medical problems effectively and appropriately. The human body and medical reality are too complicated to be fixed by modern medicine once and for all. For instance, in the present time, chronic and geriatric diseases pose perplexing challenges to modern medicine. Possibilities are always open for traditional Chinese medicine as well as the integrated traditional Chinese and modern medicine to make their valuable contributions.DOWNLOAD HISTORY | This article has been downloaded 69 times in Digital Commons before migrating into this platform.


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