scholarly journals α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7248
Author(s):  
Tran Duc Viet ◽  
Tran Dang Xuan ◽  
La Hoang Anh
Keyword(s):  
Mass Spectrometry ◽  
Xanthine Oxidase ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Gas Chromatography Mass Spectrometry ◽  
Maximum Efficiency ◽  
Ionization Mass ◽  
Skin Hyperpigmentation ◽  
Key Enzyme ◽  
Important Enzyme

Celastrus hindsii is a popular medicinal plant in Vietnam and Southeast Asian countries as well as in South America. In this study, an amount of 12.05 g of an α-amyrin and β-amyrin mixture was isolated from C. hindsii (10.75 g/kg dry weight) by column chromatography applying different solvent systems to obtain maximum efficiency. α-Amyrin and β-amyrin were then confirmed by gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR). The antioxidant activities of the α-amyrin and β-amyrin mixture were determined via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays with IC50 of 125.55 and 155.28 µg/mL, respectively. The mixture exhibited a high potential for preventing gout by inhibiting a relevant key enzyme, xanthine oxidase (XO) (IC50 = 258.22 µg/mL). Additionally, an important enzyme in skin hyperpigmentation, tyrosinase, was suppressed by the α-amyrin and β-amyrin mixture (IC50 = 178.85 µg/mL). This study showed that C. hindsii is an abundant source for the isolation of α-amyrin and β-amyrin. Furthermore, this was the first study indicating that α-amyrin and β-amyrin mixture are promising in future therapies for gout and skin hyperpigmentation.

scholarly journals Comprehensive Fractionation of Antioxidants and GC-MS and ESI-MS Fingerprints of Celastrus hindsii Leaves

Medicines ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 64 ◽  
Author(s):  
Tran Duc Viet ◽  
Tran Dang Xuan ◽  
Truong Mai Van ◽  
Yusuf Andriana ◽  
Ramin Rayee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Column Chromatography ◽  
Antioxidant Activities ◽  
Southern China ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Esi Ms

Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50–70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, terpenes, and phenols were identified. Of these compounds, α-amyrin, β-amyrin, hydrazine carboxamide, hexadecanoic acid, fucosterol, (3β)-D:C-friedours-7-en-3-ol, rutin, and 2-hydroxy-1-ethyl ester accounted for maximal quantities, whilst concentrations of other constituents were <5%. Conclusions: It is suggested that these identified compounds may greatly contribute to the antioxidant capacity of C. hindsii as well as its potential pharmaceutical properties.

scholarly journals Antioxidant, Xanthine Oxidase, α-Amylase and α-Glucosidase Inhibitory Activities of Bioactive Compounds from Rumex crispus L. Root

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3899 ◽  
Author(s):  
Truong Ngoc Minh ◽  
Truong Mai Van ◽  
Yusuf Andriana ◽  
Le The Vinh ◽  
Dang Viet Hau ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Xanthine Oxidase ◽  
Radical Scavenging ◽  
Positive Control ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Active Components ◽  
Rumex Crispus ◽  
Etoac Extract ◽  
Ic50 Values

The root of Rumex crispus L. has been shown to possess anti-gout and anti-diabetic properties, but the compounds responsible for these pharmaceutical effects have not yet been reported. In this study, we aimed to isolate and purify active components from the root of R. crispus, and to evaluate their anti-radical, anti-gout and anti-diabetic capacities. From the ethyl acetate (EtOAc) extract, two compounds, chrysophanol (1) and physcion (2), were isolated by column chromatography with an elution of hexane and EtOAc at a 9:1 ratio. Their structures were identified by spectrometric techniques including gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), X-ray diffraction analyses and nuclear magnetic resonance (NMR). The results of bioassays indicated that (1) showed stronger activities than (2). For antioxidant activity, (1) and (2) exhibited remarkable DPPH radical scavenging capacity (IC50 = 9.8 and 12.1 µg/mL), which was about two times stronger than BHT (IC50 = 19.4 µg/mL). The anti-gout property of (1) and (2) were comparable to the positive control allopurinol, these compounds exerted strong inhibition against the activity of xanthine oxidase (IC50 = 36.4 and 45.0 µg/mL, respectively). In the anti-diabetic assay, (1) and (2) displayed considerable inhibitory ability on α-glucosidase, their IC50 values (IC50 = 20.1 and 18.9 µg/mL, respectively) were higher than that of standard acarbose (IC50 = 143.4 µg/mL). Findings of this study highlight that (1) and (2) may be promising agents to treat gout and diabetes, which may greatly contribute to the medicinal properties of Rumex crispus root.

Analysis of DCM extractable components from hot-pressed hybrid poplar

Holzforschung ◽  
2012 ◽  
Vol 66 (8) ◽  
pp. 927-934 ◽  
Author(s):  
Noridah B. Osman ◽  
Armando G. McDonald ◽  
Marie-Pierre G. Laborie
Keyword(s):  
Mass Spectrometry ◽  
Hybrid Poplar ◽  
Gel Permeation Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Wood Substrate ◽  
Thermal Compression ◽  
Organic Extracts ◽  
Extractable Compounds ◽  
Soluble Material

Abstract The effects of thermal compression on the organic-soluble material of a uniform wood substrate, hybrid poplar (clone OP-367), has been studied. Poplar veneers were preconditioned to 0% or 8% moisture content and subsequently hot pressed at 150°C, 200°C, and 250°C. The dichloromethane (DCM) extracts were characterized by various hyphenated analyses [gas chromatography-mass spectrometry (GC-MS), analytical pyrolysis (Py-GC-MS), electrospray ionization-mass spectrometry (ESI-MS), and gel permeation chromatography (GPC)]. The yields and composition of extractable compounds were markedly influenced by treatment at 250°C compared to those obtained at lower temperatures. Most importantly, the organic extracts contained a significant portion of lignin-derived compounds after treatment at 250°C.

Extraction and Characterization of Gibberellins from Hordeum vulgare L. Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 183 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Mass Spectrometry ◽  
Dry Weight ◽  
Paper Electrophoresis ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Fresh Weight ◽  
Hordeum Vulgare L ◽  
Layer Chromatography

Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.

scholarly journals Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

2016 ◽  
Vol 88 (1) ◽  
pp. 281-292 ◽  
Author(s):  
CHARLENE S.C. GARCIA ◽  
CAROLINE MENTI ◽  
ANA PAULA F. LAMBERT ◽  
THIAGO BARCELLOS ◽  
SIDNEI MOURA ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mammalian Cells ◽  
Annexin V ◽  
The Body ◽  
Salvia Officinalis ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Hek 293 ◽  
Salvia Officinalis L

ABSTRACT Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identification sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia officinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, inflammation and cancer.

scholarly journals Analytical Variables Affecting Analysis of F2-Isoprostanes and F4-Neuroprostanes in Human Cerebrospinal Fluid by Gas Chromatography/Mass Spectrometry

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Hsiu-Chuan Yen ◽  
Hsing-Ju Wei ◽  
Ting-Wei Chen
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cerebrospinal Fluid ◽  
Chemical Ionization ◽  
Negative Ion ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Negative Ion Chemical Ionization ◽  
Gold Marker

F2-isoprostanes (F2-IsoPs) are a gold marker of lipid peroxidationin vivo, whereas F4-neuroprostanes (F4-NPs) measured in cerebrospinal fluid (CSF) or brain tissue selectively indicate neuronal oxidative damage. Gas chromatography/negative-ion chemical-ionization mass spectrometry (GC/NICI-MS) is the most sensitive and robust method for quantifying these compounds, which is essential for CSF samples because abundance of these compounds in CSF is very low. The present study revealed potential interferences on the analysis of F2-IsoPs and F4-NPs in CSF by GC/NICI-MS due to the use of improper analytical methods that have been employed in the literature. First, simultaneous quantification of F2-IsoPs and F4-NPs in CSF samples processed for F4-NPs analysis could cause poor chromatographic separation and falsely higher F2-IsoPs values for CSF samples with high levels of F2-IsoPs and F4-NPs. Second, retention of unknown substances in GC columns from CSF samples during F4-NPs analysis and from plasma samples during F2-IsoPs analysis might interfere with F4-NPs analysis of subsequent runs, which could be solved by holding columns at a high temperature for a period of time after data acquisition. Therefore, these special issues should be taken into consideration when performing analysis of F2-IsoPs and F4-NPs in CSF to avoid misleading results.

Sign in / Sign up

Export Citation Format

Share Document