scholarly journals Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3152
Author(s):  
Afrah Nawaz ◽  
Syed Mohsin Ali ◽  
Nosheen Fatima Rana ◽  
Tahreem Tanweer ◽  
Amna Batool ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Metallic Nanoparticles ◽  
Beta Lactams ◽  
In Vitro Antibacterial Activity ◽  
Bacteria Resistance ◽  
Host Tissues ◽  
In Vivo Assessment

Metallic nanoparticles, such as gold nanoparticles (AuNPs), have been extensively studied as drug delivery systems for various therapeutic applications. However, drug-loaded-AuNPs have been rarely explored in vivo for their effect on bacteria residing inside tissues. Ciprofloxacin (CIP) is a second-generation fluoroquinolone with a broad-spectrum of antibiotic properties devoid of developing bacteria resistance. This research is focused on the synthesis and physical characterization of Ciprofloxacin-loaded gold nanoparticles (CIP-AuNPs) and their effect on the colonization of Enterococcus faecalis in the liver and kidneys of mice. The successfully prepared CIP-AuNPs were stable and exerted enhanced in vitro antibacterial activity against E. faecalis compared with free CIP. The optimized CIP-AuNPs were administered (500 µg/Kg) once a day via tail vein to infected mice for eight days and were found to be effective in eradicating E. faecalis from the host tissues. Moreover, unlike CIP, CIP-AuNPs were non-hemolytic. In summary, this study demonstrated that CIP-AuNPs are promising and biocompatible alternative therapeutics for E.-faecalis-induced infections resistant to conventional drugs (e.g., beta-lactams and vancomycin) and should be further investigated.

Effect of Plasmonic Gold Nanoparticles on Benign and Malignant Cellular Autofluorescence: A Novel Probe for Fluorescence Based Detection of Cancer

2007 ◽  
Vol 6 (5) ◽  
pp. 403-412 ◽  
Author(s):  
Ivan El-Sayed ◽  
Xiaohua Huang ◽  
Fima Macheret ◽  
Joseph Oren Humstoe ◽  
Randall Kramer ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Metallic Nanoparticles ◽  
Noble Metal Nanoparticles ◽  
Live Cells ◽  
Epithelial Cell Line ◽  
Optical Probes ◽  
Oral Squamous Carcinoma ◽  
Absorption Of Light

Due to the strong surface fields of noble metal nanoparticles, absorption and scattering of electromagnetic radiation is greatly enhanced. Noble metallic nanoparticles represent potential novel optical probes for simultaneous molecular imaging and photothermal cancer therapy using the enhanced scattering and absorption of light. Further, gold nanoparticles can affect molecular fluorescence via chemical, electronic, or photonic interactions. Live cells generate fluorescence due to intracellular and extracellular molecules. Differences in the biochemical composition between healthy and malignant cells can be exploited in vivo to help identify cancer spectroscopically. The interaction of gold nanoparticles with cellular autofluorescence has not yet been characterized. We hypothesized that gold nanoparticles delivered to live cells in vitro would alter cellular autofluorescence and may be useful as a novel class of contrast agent for fluorescence based detection of cancer. The fluorescence of two fluorophores that are responsible for tissue autofluorescence, NADH and collagen, and of two oral squamous carcinoma cell lines and one immortalized benign epithelial cell line were measured in vitro. Gold nanoparticles of different shapes, both spheres and rods, quenched the fluorescence of the soluble NADH and collagen. Reduction of NADH fluorescence was due to oxidation of NADH to NAD+ catalyzed by gold nanoparticles (results we previously published). Reduction of collagen fluorescence appears due to photonic absorption of light. Furthermore, a mean quenching of 12/8% (p<0.00050) of the tissue autofluorescence of cell suspensions was achieved in this model when nanospheres were incubated with the live cells. Gold nanospheres significantly decrease cellular autofluorescence of live cells under physiological conditions when excited at 280nm. This is the first report to our knowledge to suggest the potential of developing targeted gold nanoparticles optical probes as contrast agents for fluorescence based diagnoses of cancer.

Biofabrication of gold nanoparticles with bone remodeling potential: an in vitro and in vivo assessment

2020 ◽  
Vol 22 (6) ◽  
Author(s):  
Sneha Singh ◽  
Archita Gupta ◽  
Irfan Qayoom ◽  
Arun Kumar Teotia ◽  
Sneha Gupta ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Bone Remodeling ◽  
In Vivo Assessment

Characterization of Photo-Oxidized Small Bore Grafts

1999 ◽  
Author(s):  
Diane L. Hern-Anderson ◽  
Anne Marie J. Hernandez ◽  
John P. Ranieri ◽  
Steven Weinberg
Keyword(s):  
Vascular Graft ◽  
Covalent Modification ◽  
Fresh Tissue ◽  
Burst Strength ◽  
Photo Oxidation ◽  
Modification Process ◽  
In Vivo Assessment

Abstract Many have hypothesized that both chemical and mechanical material properties are important in the design of a small bore vascular graft. In an attempt to address both issues, a xenogeneic graft crosslinked by photo-oxidation and covalently modified with heparin was developed. The compliance, burst strength, and suture pull strength of photo-oxidized (PhotoFix®) and heparin-modified photo-oxidized ovine carotid tissue were tested in vitro. The heparin modification process was found to have no statistically significant effect on any of these properties. The shrink temperature of fresh, photo-oxidized, and heparin-modified photo-oxidized tissue was assessed using DSC. A small increase in the shrink temperature of the heparin-modified photo-oxidized tissue over the fresh tissue was found to be statistically significant (P &lt; 0.05). In vivo assessment of unmodified, heparin-soaked, and heparin-modified PhotoFix ovine grafts indicated that covalent modification of the grafts was imperative for the graft’s success.

Biosynthesis and characterization of gold nanoparticles: Kinetics, in vitro and in vivo study

2017 ◽  
Vol 78 ◽  
pp. 553-564 ◽  
Author(s):  
Nabeel Ahmad ◽  
Sharad Bhatnagar ◽  
Ritika Saxena ◽  
Danish Iqbal ◽  
A.K. Ghosh ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
In Vivo Study

scholarly journals Gold Nanoparticles As A Modifying Agent of Ceramic-Polymer Composites

2014 ◽  
Vol 59 (3) ◽  
pp. 1005-1009 ◽  
Author(s):  
A. Sobczak-Kupiec ◽  
B. Tyliszczak ◽  
K. Krupa-Żuczek ◽  
D. Malina ◽  
M. Piątkowski ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Polymer Composites ◽  
Metallic Nanoparticles ◽  
Water Environment ◽  
Degradation Process ◽  
Degradation Behaviour ◽  
Fourier Transformed Infrared Spectroscopy ◽  
Ft Ir

Abstract Much effort has been invested in the development of biomaterials for the repair or replacement of hard tissue. The synthesis of composites based on mineral and organic constituents is nowadays extremely important for the development of materials for biomedical applications. In this paper we report the preparation and characterization of ceramic-polymer composites doped with gold nanoparticles. Properties and applications in medicine and dentistry of colloidal gold nanoparticles depends upon their size and shape. The influence of the presence of the metallic nanoparticles on the degradation process was investigated by pH and conductivity analyses of water filtrates. The nanocomposites were characterized with the use of X-ray Diffaction (XRD) and Fourier Transformed Infrared Spectroscopy (FT-IR) methods.The results of in vitro tests confirmed that it is possible to produce hydroxyapatite/polymer (HA/polymer) composites doped with gold nanoparticles (AuNPs) for medical applications. Tests proved that content of gold nanoparticles in composites had influence on degradation behaviour of HA/Polymer/AuNPs in water environment.

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

Dosimetry and Radioenhancement Comparison of Gold Nanoparticles in Kilovoltage and Megavoltage Radiotherapy using MAGAT Polymer Gel Dosimeter

2019 ◽  
Vol 9 (2Apr) ◽  
Author(s):  
S Farahani ◽  
N Riyahi Alam ◽  
S Haghgoo ◽  
M Khoobi ◽  
Gh Geraily ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
3D Visualization ◽  
Energy Levels ◽  
Polymer Gel ◽  
External Radiotherapy ◽  
Dose Enhancement ◽  
Internal Radiotherapy ◽  
Boost Radiotherapy

Background: Numerous unique characteristics of the nanosized gold, including high atomic number, low toxicity, and high biocompatibility make it one of the most appropriate nanostructures to boost radiotherapy efficacy. Many in-vivo and in-vitro investigations have indicated that gold nanoparticles (AuNPs) can significantly increase tumor injuries in low kilovoltage radiotherapy. While deep-lying tumors require much higher energy levels with greater penetration power, and investigations carried out in megavoltage energy range show contradictory results.Objective: In this study, we quantitatively assess and compare dose enhancement factors (DEFs) obtained through AuNPs under radiation of Cobalt-60 source (1.25MeV) versus Iridium-192 source (0.380 KeV) using MAGAT gel dosimeter.Material and Methods: MAGAT polymer gel in both pure and combined with 0.2 mM AuNPs was synthesized. In order to quantify the effect of energy on DEF, irradiation was carried out by Co-60 external radiotherapy and Ir-192 internal radiotherapy. Finally, readings of irradiated and non-irradiated gels were performed by MR imaging.Result: The radiation-induced R2 (1/T2) changes of the gel tubes doped with AuNPs compared to control samples, upon irradiation of beams released by Ir-192 source showed a significant dose enhancement (15.31% ±0.30) relative to the Co-60 external radiotherapy (5.85% ±0.14).Conclusion: This preliminary study suggests the feasibility of using AuNPs in radiation therapy (RT), especially in low-energy sources of brachytherapy. In addition, MAGAT polymer gel, as a powerful dosimeter, could be used for 3D visualization of radiation dose distribution of AuNPs in radiotherapy.

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