The Protective Effect of Rosmarinic Acid against Unfavorable Influence of Methylparaben and Propylparaben on Collagen in Human Skin Fibroblasts

Parabens, which are widely used in food, medicines and cosmetics, have a harmful effect on human health. People are most exposed to parabens transdermally by using cosmetic products containing these preservatives. The purpose of this study was to estimate the influence of parabens (methylparaben—MP and propylparaben—PP) on the metabolism of collagen in the human skin fibroblasts and above all, to assess whether rosmarinic acid (RA—50, 100, or 150 μM) can protect these cells from the adverse effects of parabens (0.001% MP and 0.0003% PP, 0.003% MP and 0.001% PP, and 0.01% MP and 0.003% PP). The possible mechanisms of RA action were estimated as well. Parabens decreased the expression of collagen type I and III at mRNA and protein levels, while RA (depending on the concentration) provided partial or total protection against these changes. The effective protection against the adverse effects of parabens on cell viability and proliferation was also provided by RA. The beneficial impact of RA on collagen and the fibroblasts resulted from an independent action of this compound and its interaction with parabens. This study allows us to conclude that this polyphenolic compound may protect from unfavorable health outcomes caused by lifetime human exposure to parabens contained in cosmetic products.

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The Beneficial Effect of Rosmarinic Acid on Benzophenone-3-Induced Alterations in Human Skin Fibroblasts

International Journal of Molecular Sciences ◽

10.3390/ijms222111451 ◽

2021 ◽

Vol 22 (21) ◽

pp. 11451

Author(s):

Anna Galicka ◽

Joanna Sutkowska-Skolimowska

Keyword(s):

Human Skin ◽

Rosmarinic Acid ◽

Type I Collagen ◽

Skin Fibroblasts ◽

Percutaneous Penetration ◽

Systemic Absorption ◽

Type I ◽

Human Skin Fibroblasts

Benzophenone-3 (BP-3) is one of the most widely used chemical sunscreens. The results of many in vitro and in vivo tests confirm its high percutaneous penetration and systemic absorption, which question the safety of its wide use. The aim of our research was to assess the effect of this compound on components of the skin extracellular matrix, and to investigate whether rosmarinic acid (RA) could reduce BP-3-induced changes in human skin fibroblasts. BP-3 used at concentrations of 0.1–100 µM caused a number of unfavorable changes in the level of type I collagen, decorin, sulfated glycosaminoglycans, hyaluronic acid, elastin, and expression or activity of matrix metalloproteinases (MMP-1, MMP-2), elastase and hyaluronidase. Moreover, the intracellular retention of collagen was accompanied by changes in the expression of proteins modifying and controlling the synthesis and secretion of this protein. Most importantly, RA at a concentration of 100 µM significantly reduced or completely abolished the adverse effects of BP-3. Based on these findings, it can be concluded that this polyphenol may provide effective protection against BP-3-induced disturbances in skin cells, which may have important clinical implications.

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Pinus densiflora extract protects human skin fibroblasts against UVB-induced photoaging by inhibiting the expression of MMPs and increasing type I procollagen expression

Toxicology Reports ◽

10.1016/j.toxrep.2014.08.010 ◽

2014 ◽

Vol 1 ◽

pp. 658-666 ◽

Cited By ~ 28

Author(s):

Hoe-Yune Jung ◽

Jae-Cheon Shin ◽

Seon-Min Park ◽

Na-Ri Kim ◽

Wonjung Kwak ◽

...

Keyword(s):

Human Skin ◽

Pinus Densiflora ◽

Skin Fibroblasts ◽

Type I ◽

Human Skin Fibroblasts ◽

Type I Procollagen

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Reciprocal effects of excess potassium salts in the culture medium on type I and type III collagen synthesis by human skin fibroblasts

Cell Biology International Reports ◽

10.1016/0309-1651(84)90164-4 ◽

1984 ◽

Vol 8 (6) ◽

pp. 433-440 ◽

Cited By ~ 1

Author(s):

R HATA ◽

H SUNADA ◽

K ARAI

Keyword(s):

Human Skin ◽

Collagen Synthesis ◽

Culture Medium ◽

Skin Fibroblasts ◽

Type Iii Collagen ◽

Type I ◽

Reciprocal Effects ◽

Human Skin Fibroblasts ◽

Potassium Salts ◽

Type Iii

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Stimulation of Type I Collagen Transcription in Human Skin Fibroblasts by TGF-β: Involvement of Smad 3

Journal of Investigative Dermatology ◽

10.1046/j.1523-1747.1999.00477.x ◽

1999 ◽

Vol 112 (1) ◽

pp. 49-57 ◽

Cited By ~ 299

Author(s):

Shu-Jen Chen ◽

Weihua Yuan ◽

Yasuji Mori ◽

Anait Levenson ◽

John Varga ◽

...

Keyword(s):

Human Skin ◽

Type I Collagen ◽

Skin Fibroblasts ◽

Type I ◽

Human Skin Fibroblasts ◽

Smad 3 ◽

Stimulation Of

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Root extractive from Daphne genkwa benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts

Bioscience Reports ◽

10.1042/bsr20170182 ◽

2017 ◽

Vol 37 (2) ◽

Cited By ~ 5

Author(s):

Dong Yang ◽

Jun-hua Xu ◽

Ren-jie Shi

Keyword(s):

Wound Healing ◽

Human Skin ◽

Anal Fistula ◽

Healing Time ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts ◽

Protein Levels ◽

Daphne Genkwa

Wound healing is the main problem in the therapy of anal fistula (AF). Daphne genkwa root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of D. genkwa root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wound healing times were compared and granulation tissues were collected. In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment of TNF-α (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of D. genkwa root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of TGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) and MEK/ERK signalling pathways were investigated both in vivo and in vitro. Results showed that D. genkwa root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of COL1A1, COL3A1, Timp-1, c-fos and Cyclin D1, while reduced the expression of MMP-3. Further detection presented that MEK/ERK signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from D. genkwa root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of COL1A1 and COL3A1.

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