scholarly journals Occurrence and Characteristics of Staphylococcus aureus in a Hungarian Dairy Farm during a Control Program

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 104
Author(s):  
Flóra M. Petróczki ◽  
Ákos Pásztor ◽  
Kata D. Szűcs ◽  
Károly Pál ◽  
Gábor Kardos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Large Scale ◽  
Dairy Farm ◽  
Control Program ◽  
Penicillin G ◽  
Milk Samples ◽  
Foodborne Outbreaks ◽  
Bulk Tank ◽  
Spa Types ◽  
Spa Type

In this research, our aim was to assess the occurrence of Staphylococcus aureus in a Hungarian large-scale dairy farm during the S. aureus control program conducted in the course of our studies. Furthermore, the phenotypic and genotypic properties of the isolates (type of haemolysis, antibiotic susceptibility, staphylococcal enterotoxin (SE) gene carrying ability and spa type) were determined. S. aureus was detected in all bulk tank milk samples collected during this study. Two different spa types were identified among the 17 strains isolated in the farm. A total of 14 of the 17 studied strains (82%) showed β-haemolysis on blood agar, 2/17 strains (12%) expressed double zone and 1/17 strains (6%) showed weak β-haemolysis. All strains were susceptible to most antibiotics tested (cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, tetracycline and trimethoprim/sulphamethoxazole), but all strains were resistant to penicillin G. A total of 11 of the 17 strains (65%) were found to harbour seg, sei, selm, seln, selo genes; 4/17 strains (24%) harboured sei, selm, seln, selo genes and 2/17 strains (11%) harboured sei gene. Since the new SEs/SEls can also cause foodborne outbreaks potentially and all strains were found to be resistant to penicillin G, it is essential to decrease and keep the prevalence of S. aureus low in the dairy farm and the implementation of the S. aureus control program is also highly justified. The results showed that the S. aureus count decreased by the end of our studies, so the control program was proved to be effective.

scholarly journals Rapid Detection of Mycoplasma bovis, Staphylococcus aureus and Streptococcus agalactiae in Cattle Bulk Tank Milk in Cyprus and Relations with Somatic Cell Counts

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 841
Author(s):  
Maria Liapi ◽  
George Botsaris ◽  
Costas Arsenoglou ◽  
Nikolas Markantonis ◽  
Christodoulos Michael ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Somatic Cell ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Mycoplasma Bovis ◽  
Bulk Tank Milk ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
Cell Counts ◽  
Bulk Tank

One hundred and seventy-seven (177) bulk tank milk samples were analyzed with a commercially available real-time polymerase chain reaction kit and 11 (6.21%), 41 (23.16%), and 58 (32.77%) tested positive for Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae, respectively. Statistical analysis revealed a significant relationship between the presence of S. aureus and S. agalactiae. Enumeration of somatic cells was performed in the same samples by flow cytometry. The somatic cell counts were found higher in S. aureus and S. agalactiae positive samples. No association was found between M. bovis presence and somatic cells counts. Low internal assay control Ct values were found to be related with high somatic cell counts. Noticeably, this is the first report for the presence of M. bovis in Cyprus. Therefore, its presence was confirmed by bulk tank milk culture, conventional PCR, and next generation sequencing. Furthermore, M. bovis was typed with multilocus sequencing typing and was allocated to sequence type 29 (ST 29). Real-time PCR in bulk tank milk samples is a useful tool to detect mammary infections, especially for neglected pathogens such as M. bovis.

Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in bulk tank milk, livestock and dairy-farm personnel in north-central and north-eastern Greece: Prevalence, characterization and genetic relatedness

2019 ◽  
Vol 84 ◽  
pp. 103249 ◽  
Author(s):  
Panagiotis Papadopoulos ◽  
Apostolos S. Angelidis ◽  
Theofilos Papadopoulos ◽  
Charalampos Kotzamanidis ◽  
Antonios Zdragas ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Relatedness ◽  
Dairy Farm ◽  
Bulk Tank Milk ◽  
North Central ◽  
Methicillin Resistant ◽  
North Eastern ◽  
Bulk Tank

scholarly journals Iodine content in bulk tank milk samples in relation to dairy farm size

2014 ◽  
Vol 83 (10) ◽  
pp. S9-S13 ◽  
Author(s):  
Lenka Vorlová ◽  
Lucia Hodulová ◽  
Ivana Borkovcová ◽  
Hana Přidalová ◽  
Romana Kostrhounová ◽  
...  
Keyword(s):  
Dairy Farm ◽  
Iodine Content ◽  
Dairy Farms ◽  
Raw Milk ◽  
Farm Size ◽  
Milk Samples ◽  
Significant Difference ◽  
Organic Dairy ◽  
Bulk Tank ◽  
Organic Dairy Farms

The aim of this study was to compare the iodine content in raw milk from organic and conventional dairy farms of different sizes. Milk samples were collected between 2012 and 2013, and the iodine content was determined by a Sandell-Kolthoff reaction after dry alkaline digestion of the milk samples. Comparing the iodine content in raw milk samples from small sized dairy farms (116.76 ± 46.29 μg/l) and large sized dairy farms (173.70 ± 35.42 μg/l), a significant difference in iodine content was observed (P ≤ 0.05). The lowest values were found in small and medium dairy farms, 45.30 μg/l and 40.46 μg/l, respectively. High variability (112.92 ± 94.74 μg/l) in the iodine content was detected in raw milk from medium sized dairy farms. When considering milk samples from organic dairy farms (119.29 μg /l ± 40.37) vs. conventional dairy farms (136.55 μg/l ± 42.91), no significant difference was detected. These results indicate higher iodine content in milk from large dairy farms regardless of conventional or organic farming methods.

scholarly journals Findings of methicillin-resistant strains of Staphylococcus aureus in livestock

2010 ◽  
Vol 27 (Special Issue 2) ◽  
pp. 36-41
Author(s):  
Z. Šťástková ◽  
S. Karpíšková ◽  
R. Karpíšková
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Potential Hazard ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Milk Samples ◽  
Genes Encoding ◽  
Resistant Strains ◽  
Bulk Tank

The aim of our study was to determine the occurrence of methicillin resistant Staphylococcus aureus (MRSA) at dairy farms in the Czech Republic. Altogether 1061 samples from 95 farms were examined. The samples analysed were milk (individual and bulk tank milk samples), animal swabs and swabs from the farm environment. In total, 299 S. aureus isolates were obtained, of which 23 were MRSA. These MRSA isolates originated from three farms (13 isolates from farm A and 5 isolates from each of farms B and C). All MRSA isolates carried the mecA gene while none of them carried the genes for PVL, TSST-1 and exfoliatins. Only the isolates from goat farm C were positive for the genes encoding enterotoxins. By SCCmec typing, the strains were classified as community-associated MRSA carrying SCCmec IV or V. This study revealed that animals can be an important source of methicillin resistant staphylococci and represent a potential hazard of further spread.

scholarly journals Occurrence of enterotoxin genes and macrorestriction analysis of Staphylococcus aureus isolated from bovine mastitis and bulk-tank milk samples in Italy. An epidemiological study

2004 ◽  
Vol 3 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Andrea Serraino ◽  
Leonardo Alberghini ◽  
Maria Cristina Fontana ◽  
Cosima Annemüller ◽  
Christoph Lämmler ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Epidemiological Study ◽  
Bovine Mastitis ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Enterotoxin Genes ◽  
Bulk Tank

scholarly journals A study on Methicillin resistant Staphylococcus aureus mastitis in dairy cows

2014 ◽  
Vol 6 (2) ◽  
pp. 356-361 ◽  
Author(s):  
D. Chandrasekaran ◽  
P. Venkatesan ◽  
K. G. Tirumurugaan ◽  
A. P. Nambi ◽  
P. S. Thirunavukkarasu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cows ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clinical Mastitis ◽  
Penicillin G ◽  
Diffusion Method ◽  
Isolation And Identification ◽  
Methicillin Resistant ◽  
Milk Samples ◽  
Agar Disc

Methicillin-resistant Staphylococcus aureus (MRSA) poses a serious problem in dairy animals suffering from mastitis. The study was carried out to evaluate the incidence of Methicillin resistant S. aureus from clinical mastitis milk samples and their antibiotic resistance profile and characterised with respect to the molecular features that contributed to the resistance in these pathogens. Isolation and identification of Methicillin resistant S. aureus were performed from acute clinical mastitis samples. The isolates were tested using agar disc diffusion method for their antimicrobial susceptibility and modified resazurin assay micro dilution technique for MIC to 8 different antimicrobial drugs. A total of 235 clinical mastitis milk samples from dairy cows were cultured for incidence of S. aureus. Methicillin resistant S. aureus was isolated from a total of 12 (44.25%) of the 116 S. aureus samples. Based on the antimicrobial sensitivity and MIC results, MRSA isolates were found sensitive to gentamicin, enrofloxcain, amoxicillin+sulbactam, ceftriaxone and resistant to amoxicillin, oxytetracycline, penicillin G and oxacillin. Most of MRSA isolates were found to be multi-drug resistant. MRSA alert kit test and mecA and blaZ target gene PCR were found to be useful in the confirmation of MRSA.

scholarly journals Antimicrobial Resistance Profiles of Staphylococcus Aureus Isolated from Meat Carcasses and Bovine Milk in Abattoirs and Dairy Farms of the Eastern Cape, South Africa

2018 ◽  
Author(s):  
Ezekiel Green ◽  
Abongile Pekana
Keyword(s):  
South Africa ◽  
Staphylococcus Aureus ◽  
Bovine Milk ◽  
Dairy Farms ◽  
Penicillin G ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
Biochemical Tests ◽  
Milk Samples ◽  
Antibiotic Susceptibility Test

Background: Staphylococcus aureus (S. aureus) occasionally threatens the life of the host as a persistent pathogen even though it is normal flora of humans and animals. We characterized drug resistance in S. aureus isolated from animal carcasses and milk samples from the abattoirs and dairy farms in the Eastern Cape Province. Methods: A 1000 meat swab samples and 200 raw milk samples were collected from selected abattoirs and dairy farms in the Eastern Cape Province, South Africa. S. aureus was isolated and positively identified using biochemical tests and confirmed by molecular methods. Antibiotic susceptibility test against 14 different antibiotics was performed against all isolates. Antibiotic resistance genes were also detected. Results: Of the 1200 samples collected, 134 (11.2%) samples were positive for S. aureus. Resistance ranged from 71.6% for penicillin G to 39.2% for tetracycline. Resistance gene (blaZ) was detected in 13 (14.9%), while msrA was found in 31 (52.5%) of S. aureus isolates. Conclusions: The present result shows the potential dissemination of multidrug-resistant S. aureus strains in the dairy farms and abattoirs in the Eastern Cape. Therefore, this implies that the organism may rapidly spread through food and pose serious public health risk

Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep

2004 ◽  
Vol 101 (2) ◽  
pp. 101-107 ◽  
Author(s):  
D Scherrer ◽  
S Corti ◽  
J.E Muehlherr ◽  
C Zweifel ◽  
R Stephan
Keyword(s):  
Staphylococcus Aureus ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank ◽  
Genotypic Characteristics

scholarly journals Isolation and characterization of staphylococcus aureus from two large-scale food poisoning outbreaks in Vietnam

2020 ◽  
pp. 139-147
Author(s):  
Lam Quoc Hung ◽  
◽  
Huong Minh Nguyen ◽  
Ta Thi Yen ◽  
Le Vinh Hoa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Food Production ◽  
Large Scale ◽  
Virulence Genes ◽  
Food Poisoning ◽  
Isolation And Characterization ◽  
Food Borne ◽  
Different Strains ◽  
Spa Type

In Vietnam and around the world, Staphylococcus aureus remains a major hazard of food safety and food poisoning. S. aureus is present in many places and easily contaminates food production during processing chains. In this study, we successfully isolated S. aureus strains from suspected samples of two food borne poisoning outbreaks in Ha Giang and Vinh Phuc in 2017 and 2018, respectively. The collected samples were examined for presence of staphylococcal enterotoxins (SEs) by using 3MTMTECRATM Staph Enterotoxin kit, from there all the samples were positive with SEs. Different strains of S. aureus were isolated and then confirmed by MALDI-TOF technique. Those strains then were stored in Brain heart solution with 15% glycerol until further analysis. Our results identified three STs, ST96, ST88 (spa type t7558), and ST72 (spa type t3092), were responsible for two outbreaks. Two virulence genes detected from the above strains were sea and sec. Furthermore, these strains are test for antibiotic resistance susceptibility with commonly antibiotics. Penicillin are found to be resisted by all three STs, in particularly, ST96 and ST88 are both resistant to erythromycin while ST72 is resistant to gentamicin. Taken together, our study highlights the usefulness of molecular characterization to study and monitor bacterial pathogens associated with food poisoning outbreaks in Vietnam.

Sign in / Sign up

Export Citation Format

Share Document