scholarly journals Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1527
Author(s):  
Andreja Jungić ◽  
Vladimir Savić ◽  
Josip Madić ◽  
Ljubo Barbić ◽  
Besi Roić ◽  
...  
Keyword(s):  
Blood Serum ◽  
Current Knowledge ◽  
Swine Influenza Virus ◽  
Swine Influenza ◽  
M Gene ◽  
Serum Samples ◽  
Tissue Samples ◽  
The Matrix ◽  
Na Gene

In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one subtype were detected in 62.19% of blood serum samples. Detection of swIAV antigen was performed by IHC and detected in 8 of 28 lung samples collected post-mortem. The matrix (M) gene was detected in nine of one hundred and forty-two lung tissue samples and in seven of twenty-nine nasopharyngeal swabs. Phylogenetic analysis of amplified HA and NA gene fragments in Croatian isolates suggests the presence of swIAV H1avN1av.

Antigenic and genetic characterization of a European avian-like H1N1 swine influenza virus from a boy in China in 2011

2012 ◽  
Vol 158 (1) ◽  
pp. 39-53 ◽  
Author(s):  
Xian Qi ◽  
Lunbiao Cui ◽  
Yongjun Jiao ◽  
Yuning Pan ◽  
Xihan Li ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Genetic Characterization ◽  
Swine Influenza Virus ◽  
Swine Influenza

Identification and characterization of human dendritic cell subsets in the steady state: a review of our current knowledge

2016 ◽  
Vol 64 (4) ◽  
pp. 833-847 ◽  
Author(s):  
Vineet Indrajit Patel ◽  
Jordan Patrick Metcalf
Keyword(s):  
Steady State ◽  
Current Knowledge ◽  
Cell Types ◽  
Lymphoid Tissues ◽  
Transcriptional Level ◽  
Functional Abilities ◽  
Tissue Samples ◽  
To Come ◽  
Antigen Presenting

Dendritic cells (DC) are generally categorized as a group of rare antigen presenting cells that are to the crucial development of immune responses to pathogens and also of tolerance to self-antigens. Therefore, having the ability to identify DC in specific tissues and to test their functional abilities in the steady state are scientific gaps needing attention. Research on primary human DC is lacking due to their rarity and the difficulty of obtaining tissue samples. However, recent findings have shown that several different DC subsets exist, and that these subsets vary both by markers expressed and functions depending on their specific microenvironment. After discriminating from other cell types, DC can be split into myeloid and plasmacytoid fractions. While plasmacytoid DC express definite markers, CD123 and BDCA-2, myeloid DC encompass several different subsets with overlapping markers expressed. Such markers include the blood DC antigens BDCA-1 and BDCA-3, along with Langerin, CD1a and CD14. Marker specificity is further reduced when accounting for microenvironmental differences, as observed in the blood, primary lymphoid tissues, skin and lungs. The mixed leukocyte reaction (MLR) has been used to measure the strength of antigen presentation by specific DC subsets. Surface markers and MLR require standardization to enable consistent identification of and comparisons between DC subsets. To alleviate these issues, researchers have begun comparing DC subsets at the transcriptional level. This has allowed degrees of relatedness to be determined between DC in different microenvironments, and should be a continued area of focus in years to come.

Characterization of a newly emerged genetic cluster of H1N1 and H1N2 swine influenza virus in the United States

Virus Genes ◽  
2009 ◽  
Vol 39 (2) ◽  
pp. 176-185 ◽  
Author(s):  
Amy L. Vincent ◽  
Wenjun Ma ◽  
Kelly M. Lager ◽  
Marie R. Gramer ◽  
Juergen A. Richt ◽  
...  
Keyword(s):  
United States ◽  
Influenza Virus ◽  
Swine Influenza Virus ◽  
Swine Influenza ◽  
The United States ◽  
Genetic Cluster

Isolation and molecular characterization of an H5N1 swine influenza virus in China in 2015

2017 ◽  
Vol 163 (3) ◽  
pp. 701-705
Author(s):  
Haibo Wu ◽  
Fan Yang ◽  
Rufeng Lu ◽  
Lihua Xu ◽  
Fumin Liu ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Molecular Characterization ◽  
Swine Influenza Virus ◽  
Swine Influenza

scholarly journals Characterization of a novel oil-in-water emulsion adjuvant for swine influenza virus and Mycoplasma hyopneumoniae vaccines

Vaccine ◽  
2015 ◽  
Vol 33 (25) ◽  
pp. 2903-2908 ◽  
Author(s):  
A. Galliher-Beckley ◽  
L.K. Pappan ◽  
Rachel Madera ◽  
Y. Burakova ◽  
A. Waters ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Swine Influenza Virus ◽  
Swine Influenza ◽  
Mycoplasma Hyopneumoniae ◽  
Water Emulsion ◽  
Oil In Water ◽  
Oil In Water Emulsion

Genetic characterization of H1N1, H1N2 and H3N2 swine influenza virus in Thailand

2008 ◽  
Vol 153 (6) ◽  
pp. 1049-1056 ◽  
Author(s):  
Salin Chutinimitkul ◽  
Nattakarn Thippamom ◽  
Sudarat Damrongwatanapokin ◽  
Sunchai Payungporn ◽  
Roongroje Thanawongnuwech ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Genetic Characterization ◽  
Swine Influenza Virus ◽  
Swine Influenza

scholarly journals Laboratory characterization of a swine influenza virus isolated from a fatal case of human influenza.

1989 ◽  
Vol 27 (6) ◽  
pp. 1413-1416 ◽  
Author(s):  
P A Rota ◽  
E P Rocha ◽  
M W Harmon ◽  
V S Hinshaw ◽  
M G Sheerar ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Fatal Case ◽  
Swine Influenza Virus ◽  
Swine Influenza ◽  
Human Influenza ◽  
Laboratory Characterization

scholarly journals Diagnosis and Molecular Characterization ofMycobacterium aviumsubsp.paratuberculosisfrom Dairy Cows in Colombia

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
J. A. Fernández-Silva ◽  
A. Abdulmawjood ◽  
M. Bülte
Keyword(s):  
Molecular Characterization ◽  
Tandem Repeats ◽  
Dairy Herd ◽  
Variable Number ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dairy Herds ◽  
Serum Samples ◽  
Tissue Samples ◽  
Fecal Samples

The objective of this study was the serological, bacteriological and molecular diagnosis, as well as the molecular characterization ofMycobacterium aviumsubsp.paratuberculosis(Map) in adult cows of five Colombian dairy herds. Serum samples were tested by an indirect absorbed enzyme–linked immunosorbent assay (ELISA-C). All fecal samples were tested by pooled culture. After that, fecal samples of Map positive pools were tested individually by culture and polymerase chain reaction (PCR). In one herd, slurry and tissue samples from one animal were also taken and tested by PCR and culture. Map isolates were analyzed by the Multilocus Short Sequence Repeat (MLSSR) and the Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTR) methods. ELISA produced positive results in 1.8% (6/329) of the animals and 40% (2/5) of the herds. Four fecal, two tissue, and two slurry samples from a herd were Map positive by culture and PCR. MLSSR and MIRU-VNTR revealed two different strain profiles among eight Map isolates recovered. This study reports the first molecular characterization of Map in one dairy herd in Colombia, the limitations for individual diagnosis of subclinical Map infections in cattle, and the usefulness of pooled fecal samples and environmental sampling for Map diagnosis.

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