Prevalence of Aggregatibacter actinomycetemcomitans and Periodontal Findings among 14 to 15-Year Old Danish Adolescents: A Descriptive Cross-Sectional Study

Aggregatibacter actinomycetemcomitans (Aa) is a keystone pathogen associated with periodontitis in adolescents. The knowledge on the prevalence of Aa and periodontitis among adolescents in Northern Europe is sparse. A total of 525 14- to 15-year-old adolescents from the municipality of Aarhus, Denmark, underwent a full-mouth clinical examination. Plaque score (PS), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment loss (CAL) were recorded. Subgingival plaque samples (SPS) and stimulated saliva samples (SSS) were collected and analyzed for the presence of JP2 and non-JP2 genotypes of Aa using real-time PCR. A total of 70 (13.3%) individuals were positive for Aa, with 17 found in SPS, 19 in SSS, and 35 in both. The highly leukotoxic JP2 genotype of Aa was not detected. The individuals positive for Aa in both SPS and SSS had poorer periodontal outcomes (PPD and CAL) than individuals without Aa and individuals carrying Aa in either SPS or SSS only. In conclusion, 13% of 14- to 15-year-old Danish adolescents were positive for Aa, and the presence of Aa in both SPS and SSS was associated with poorer periodontal outcomes.

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Pre-schoolers’ tooth brushing behaviour and association with their oral health: a cross sectional study

BMC Oral Health ◽

10.1186/s12903-021-01643-8 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Iqra Muhammad Khan ◽

Shani Ann Mani ◽

Jennifer Geraldine Doss ◽

Mahmoud Danaee ◽

Lydia Yi Li Kong

Keyword(s):

Preschool Children ◽

Oral Health ◽

Dental Caries ◽

Parental Involvement ◽

Cross Sectional Study ◽

Parental Supervision ◽

Gingival Index ◽

Sectional Study ◽

Cross Sectional ◽

Plaque Score

Abstract Background Toothbrushing is an important yet neglected behaviour that affects the oral health of preschool children. Little is reported on parental supervision, an essential aspect of routine effective toothbrushing in this age group. The aim of this study was to evaluate pre-schoolers’ toothbrushing behaviour including parental involvement and its association with their oral health. Methods This was a cross-sectional study. A total of 92 preschool children (4–6 years) were invited to participate with their parents/guardians. Nine parameters of toothbrushing behaviour were assessed from parental responses (questionnaire) and observation of child and parents/guardians (video recording). Oral examination included recording plaque, gingival and dental caries indices. BORIS software was used to assess toothbrushing parameters and Smart PLS was used to perform association with a second-generation multivariate analysis to create models with and without confounding factors. Results Girls were slightly more (53%) than boys (47%). Children aged 4 years were slightly more in number (38%), followed by 6-year-olds and 5-year-olds. Nearly, 90% parents had tertiary education and 46% had more than 2 children. Differences were recorded in the reported and observed behaviour. Thirty-five percent parents/guardians reported using pea-size toothpaste amount but only 28% were observed. Forty percent reported to brush for 30 s–1 min, however 51% were observed to brush for 1–2 min. Half the children were observed to use fluoridated toothpaste (F < 1000 ppm) under parental supervision (11%). The mean (SD) plaque score reduction after toothbrushing was 10.80 (2.46), mean pre-brushing plaque score was 90.3 (10.2), mean gingival index was 0.89 (0.65) and mean dental caries status (ICDAS(1–6)) was 18.87 (12.39). Toothbrushing behaviour in terms of toothbrushing technique, duration, pattern and frequency, toothbrush type and grip type, toothpaste type and amount, post-brushing mouth rinsing and parental involvement contributed significantly to plaque score change (86%), dental caries status (73%), gingival index (66%) and pre-brushing plaque score (31%). The significant confounding variables had a small influence on oral health of preschool children. Conclusions Preschool children’s toothbrushing behaviour was inadequate while their oral health was poor, with a significant association between the two parameters.

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Oral health status among long-term hospitalized adults: A cross sectional study.

10.7287/peerj.preprints.178 ◽

2013 ◽

Author(s):

Leon Bilder ◽

Nirit Yavnai ◽

Avi Zini

Keyword(s):

Health Status ◽

Oral Health ◽

Cross Sectional Study ◽

Oral Health Status ◽

Sectional Study ◽

Cross Sectional ◽

Term Care ◽

Plaque Score ◽

Dental Professional

Background: Many Long-Term Care (LTC) patients suffer from dental neglect due to difficulties in achieving appropriate dental professional care; although oral health has important influence on the quality of life among them. Dental care of the long term institutionalized adults is often limited to emergency and first aid care and there is insufficient data regarding oral health status in this population.Objectives: To describe the oral health status of the long-term hospitalized adults. Materials and methods: A cross-sectional study including clinical oral examinations was carried out among institutionalized LTC patients aged 18 and older in a geriatric - psychiatric Hospital in Israel. Main outcome measures were: edentulousness, presence of dentures, mucosal findings, number of teeth, number of functional teeth, level of dental hygiene and, dental caries. Results: Subjects’ mean age was 65 years; 31.3% of the patients were edentulous, and only 14% had partial or full dentures. Only 17.2% were caries free. Females had significantly higher number of caries cavitation than men (p=0.044). The number of caries cavitation was higher among patients with higher plaque scores (p<0.001) and when taking Clonex (p=0.018). Number of residual teeth in mouth was higher in the low plaque score group (p<0.001). Carious teeth percentage was higher among the high plaque score group (p<0.001)

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Validating gingival surface temperature as an alternative tool in the diagnosis of periodontal disease activity: An observational clinical trial

Journal of Dental Research Dental Clinics Dental Prospects ◽

10.15171/joddd.2019.019 ◽

2019 ◽

Vol 13 (2) ◽

pp. 123-127

Author(s):

Sumanth Gunupati ◽

Hasya Sappiti ◽

Sreenivas Nagarakanti ◽

BV Ramesh Reddy ◽

Vijay Kumar Chava

Keyword(s):

Pilot Study ◽

Periodontal Disease ◽

Surface Temperature ◽

Disease Activity ◽

Diagnostic Tool ◽

Pocket Depth ◽

Clinical Attachment Loss ◽

Cross Sectional ◽

Probing Pocket Depth ◽

Pilot Clinical Study

Background. Elevated temperature has been recognized as an inflammatory sign. It is the only indication that can be both objectively and quantitatively evaluated and is considered as a potential indicator of periodontal disease. Assessing gingival surface temperature (GST) could be a diagnostic parameter to determine periodontal health. This pilot clinical study aimed to validate gingival surface temperature (GST) as a clinical diagnostic tool to measure periodontal disease activity by correlating with the periodontal inflamed surface area (PISA). Methods. A cross-sectional mono-center pilot study was conducted with a convenient sample of 50 participants with a mean age of 34.14±13.7 years. Clinical parameters such as probing pocket depth (PPD) clinical attachment loss (CAL) and bleeding on probing (BOP) were measured. GST was recorded with a single lead of the bedside patient monitor and correlated with PISA. Results. The results showed a positive correlation between PISA and GST (P=0.46). Conclusion. This study showed a rise in GST of inflamed sites, but the results did not support the hypothesis that increased GST is an indicator of periodontal disease. As this is a pilot study, further studies with more larger sample sizes need to be undertaken to confirm its use as a diagnostic tool in clinical trials.

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Peer Review #1 of "Candidal carriage in saliva and subgingival plaque among smokers and non-smokers with chronic periodontitis—a cross-sectional study (v0.2)"

10.7287/peerj.8441v0.2/reviews/1 ◽

2020 ◽

Author(s):

IA Taher

Keyword(s):

Peer Review ◽

Chronic Periodontitis ◽

Cross Sectional Study ◽

Subgingival Plaque ◽

Sectional Study ◽

Cross Sectional

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Investigation of Salivary RANKL and OPG Levels in Periodontitis Patients at Hospital Universiti Sains Malaysia

European Journal of Dentistry ◽

10.1055/s-0041-1731930 ◽

2021 ◽

Author(s):

Saba Asif ◽

Basaruddin Ahmad ◽

Syed Ameer Hamza ◽

Haslina Taib ◽

Nur Karyatee Kassim ◽

...

Keyword(s):

Cross Sectional Study ◽

Periodontal Pocket ◽

Pocket Depth ◽

Cross Sectional ◽

Plaque Score ◽

Mean Values ◽

Severe Periodontitis ◽

Whole Saliva ◽

Elisa Technique ◽

Unstimulated Whole Saliva

Abstract Background This study was aimed to determine the levels of salivary receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) and its association with periodontal status among periodontitis patients. Patients and Methods A cross-sectional study was designed and performed at the Dental Clinic, Hospital Universiti Sains Malaysia (HUSM). Random sampling was employed to identify 88 participants into three groups: 30 mild periodontitis, 30 moderate to severe periodontitis, and 28 healthy (nonperiodontitis) patients. Periodontal parameters: periodontal pocket depth (PPD), clinical attachment level (CAL), plaque score (PS), and gingival bleeding index (GBI) were recorded. In total, 4 mL of unstimulated whole saliva was collected to determine the levels of salivary RANKL and OPG proteins by using ELISA technique. Data were analyzed by using SPSS software version 24.0. Results Mean values for PPD (5.3 ± 0.5) and CAL (5.6 ± 0.5) were observed higher for moderate to severe periodontitis as compared with values (4.4 ± 0.2) (4.5 ± 0.2) in mild periodontitis patients. The mean salivary RANKL and OPG was 0.23 ± 0.07 ng/mL and 1.78 ± 0.70 ng/mL respectively in moderate to severe periodontitis. Only salivary RANKL levels were significantly and positively correlated with all the clinical periodontal parameters. Conclusion The levels of salivary RANKL were higher as opposed to lower OPG levels in periodontitis patients in contrast to healthy (nonperiodontitis) patients. RANKL levels were significantly associated with the periodontal parameters. Therefore, we can conclude that RANKL can potentially aid as an adjunctive diagnostic protein in evaluating periodontal disease.

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Clinical signs, symptoms, perceptions and impact on quality of life of patients suffering peri-implant diseases: a university-representative cross-sectional study

10.1101/2020.06.08.20125229 ◽

2020 ◽

Author(s):

Mario Romandini ◽

Cristina Lima ◽

Ignacio Pedrinaci ◽

Ana Araoz ◽

Maria Costanza Soldini ◽

...

Keyword(s):

Quality Of Life ◽

Clinical Signs ◽

Cross Sectional Study ◽

Pocket Depth ◽

Cross Sectional ◽

Probing Pocket Depth ◽

History Of ◽

A Minor ◽

Health Diagnosis

AbstractAimTo study the symptoms and perception reported by patients with peri-implant diseases, as well as their signs and their potential impact on the oral health quality of life.Materials and Methods240 randomly selected patients were invited to participate. As part of the history assessment, the patient OHIP-14Sp was evaluated together with, for each implant, the patient perception regarding the peri-implant health status and the history of pain, spontaneous discomfort, bleeding, suppuration, swelling and discomfort during brushing. As part of the clinical examination, the following potential signs of peri-implant diseases were collected: probing pocket depth (PPD), mucosal dehiscence (MD), extent of BoP, presence of SoP, and visual signs of redness and swelling. Those parameters were analyzed in relation to the actual peri-implant health diagnosis.Results99 patients with a total of 458 dental implants were studied. Even in case of peri-implantitis, 88.89% of the implants were perceived by the patients as healthy. The total OHIP-14Sp sum score did not differ in relation to the peri-implant health diagnosis. Increased reports of spontaneous discomfort, bleeding, swelling and discomfort during brushing were observed in presence of disease. However, only a minor proportion of implants with peri-implant diseases presented symptoms. PPD≥6 mm was more frequent in diseased than in healthy implants(p<0.01), while PPD≥8 in pre-periimplantitis/peri-implantitis than in healthy/mucositis implants(p<0.01). Implants with peri-implantitis showed higher MD than implants without peri-implantitis(p<0.01).ConclusionsPeri-implant diseases are in most cases asymptomatic and not perceived by the patients. Despite being unable to accurately discriminate between peri-implant mucositis and peri-implantitis, PPD and MD resulted as the only two clinical signs associated with pre-periimplantitis/peri-implantitis.

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Association between Sleep Quality and Duration and Periodontal Disease among University Students: A Cross-Sectional Study

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17093034 ◽

2020 ◽

Vol 17 (9) ◽

pp. 3034

Author(s):

Md Monirul Islam ◽

Daisuke Ekuni ◽

Naoki Toyama ◽

Ayano Taniguchi-Tabata ◽

Kota Kataoka ◽

...

Keyword(s):

Periodontal Disease ◽

University Students ◽

Sleep Quality ◽

Sleep Duration ◽

Cross Sectional Study ◽

Poor Sleep ◽

Sectional Study ◽

Pocket Depth ◽

Cross Sectional ◽

Japanese University

The purpose of this cross-sectional study was to investigate the association between sleep quality and duration, and periodontal disease among a group of young Japanese university students. First-year students (n = 1934) at Okayama University who voluntarily underwent oral health examinations were included in the analysis. Sleep quality and duration were assessed by the Japanese version of the Pittsburgh Sleep Quality Index. Dentists examined Oral Hygiene Index-Simplified (OHI-S), probing pocket depth (PPD), and percentage of sites with bleeding on probing (BOP). Periodontal disease was defined as presence of PPD ≥ 4 mm and BOP ≥ 30%. Overall, 283 (14.6%) students had periodontal disease. Poor sleep quality was observed among 372 (19.2%) students. Mean (± standard deviation) sleep duration was 7.1 ± 1.1 (hours/night). In the logistic regression analysis, periodontal disease was significantly associated with OHI-S (odds ratio [OR]: 2.30, 95% confident interval [CI]: 1.83–2.90; p < 0.001), but not sleep quality (OR: 1.09, 95% CI: 0.79–1.53; p = 0.577) or sleep duration (OR: 0.98, CI: 0.87–1.10; p = 0.717). In conclusion, sleep quality and duration were not associated with periodontal disease among this group of young Japanese university students.

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Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1781 ◽

2015 ◽

Vol 16 (11) ◽

pp. 915-920 ◽

Cited By ~ 3

Author(s):

Vinayak Mahableshwar Joshi ◽

Kishore Gajanan Bhat ◽

Sandeep Suresh Katti ◽

Manohar Suresh Kugaji ◽

Preeti Shivaji Ingalgi

Keyword(s):

Human Cytomegalovirus ◽

Chronic Periodontitis ◽

Cross Sectional Study ◽

Clinical Parameters ◽

Pocket Depth ◽

Clinical Attachment Loss ◽

Cross Sectional ◽

Exact Test ◽

Healthy Group ◽

Periodontal Infection

ABSTRACT Objective The identification of new uncultured species and viruses supports the possibility of combination of the herpesvirus- bacterial periodontal infection for periodontitis. The paucity of data and studies with larger sample size in Indian subjects provides an unclear picture of the presence of the herpesvirus in this population. Materials and methods This was a cross-sectional study consisting of 100 each in the healthy group and chronic periodontitis (CP) group. The subgingival plaque was collected and polymerase chain reaction was performed post deoxyribonucleic acid (DNA) extraction by using specific primers for human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). The data were analyzed using Fisher's exact test, Mann-Whitney U test and Spearman's coefficient correlation. Results Human cytomegalovirus and EBV viruses were significantly higher in the CP group as compare to the healthy group. A higher percentage of those with CMV positive had EBV also positive (28.3%) compared to only 9.1% of CMV negative being EBV positive in the CP group. When both the healthy and CP group in total was compared, there was a significant correlation with all clinical parameters. Conclusion Both the viruses dominated in disease as compared to health were similar to the earlier findings. The CP group had higher pocket depth and clinical attachment loss in the virus positive subjects. These findings could suggest that virus serves as a prelude to the disease and the combination of the two viruses could play a role in the pathogenesis. How to cite this article Joshi VM, Bhat KG, Katti SS, Kugaji MS, Ingalgi PS. Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis. J Contemp Dent Pract 2015;16(11):915-920.

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Prevalence of Peri-Implantitis: A Multi-Centered Cross-Sectional Study on 248 Patients

Dentistry Journal ◽

10.3390/dj8030080 ◽

2020 ◽

Vol 8 (3) ◽

pp. 80

Author(s):

Tommaso Weinstein ◽

Tommaso Clauser ◽

Massimo Del Fabbro ◽

Matteo Deflorian ◽

Andrea Parenti ◽

...

Keyword(s):

Bone Loss ◽

Supportive Therapy ◽

Cross Sectional Study ◽

Smoking Habits ◽

Sectional Study ◽

Related Factors ◽

Pocket Depth ◽

Cross Sectional ◽

History Of ◽

Implant Therapy

The aim of this multicenter cross-sectional study was to determine the prevalence of peri-implantitis and to assess its association with several patient- and implant-related factors. Patients with at least one implant, who came for a recall visit to one of the four centers over a period of five months, were enrolled. Presence of peri-implantitis (defined as bleeding on probing, exudate/suppuration, bone loss > 0.2 mm/year and increased pocket depth) and several other variables (e.g., smoking habits, history of periodontitis, diabetes) were recorded. Out of 248 enrolled patients (1162 implants), 10 patients had at least one implant with peri-implantitis (4.03%); a total of 14 implants were affected (1.20%). A statistically significant association between peri-implantitis and diabetes was found (OR 8.65; CI: 1.94–38.57). Smoking more than 10 cigarettes per day (OR: 0.53; CI 0.03–9.45) and history of periodontitis (OR: 2.42; CI: 0.49–11.89) were not found to be statistically associated with peri-implantitis. Even if implant therapy is a consolidated treatment, biological complications do happen. Strict supportive therapy recalls could lead to lower rates of peri-implantitis and earlier diagnosis.

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