Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis

2015 ◽  
Vol 16 (11) ◽  
pp. 915-920 ◽  
Author(s):  
Vinayak Mahableshwar Joshi ◽  
Kishore Gajanan Bhat ◽  
Sandeep Suresh Katti ◽  
Manohar Suresh Kugaji ◽  
Preeti Shivaji Ingalgi
Keyword(s):  
Human Cytomegalovirus ◽  
Chronic Periodontitis ◽  
Cross Sectional Study ◽  
Clinical Parameters ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Exact Test ◽  
Healthy Group ◽  
Periodontal Infection

ABSTRACT Objective The identification of new uncultured species and viruses supports the possibility of combination of the herpesvirus- bacterial periodontal infection for periodontitis. The paucity of data and studies with larger sample size in Indian subjects provides an unclear picture of the presence of the herpesvirus in this population. Materials and methods This was a cross-sectional study consisting of 100 each in the healthy group and chronic periodontitis (CP) group. The subgingival plaque was collected and polymerase chain reaction was performed post deoxyribonucleic acid (DNA) extraction by using specific primers for human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). The data were analyzed using Fisher's exact test, Mann-Whitney U test and Spearman's coefficient correlation. Results Human cytomegalovirus and EBV viruses were significantly higher in the CP group as compare to the healthy group. A higher percentage of those with CMV positive had EBV also positive (28.3%) compared to only 9.1% of CMV negative being EBV positive in the CP group. When both the healthy and CP group in total was compared, there was a significant correlation with all clinical parameters. Conclusion Both the viruses dominated in disease as compared to health were similar to the earlier findings. The CP group had higher pocket depth and clinical attachment loss in the virus positive subjects. These findings could suggest that virus serves as a prelude to the disease and the combination of the two viruses could play a role in the pathogenesis. How to cite this article Joshi VM, Bhat KG, Katti SS, Kugaji MS, Ingalgi PS. Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis. J Contemp Dent Pract 2015;16(11):915-920.

scholarly journals Association of haptoglobin and natural resistance-associated macrophage protein 1 alleles with heme-consuming periodontal pathogens in chronic periodontitis and peri-implantitis: A pilot study

2020 ◽  
Vol 12 (1) ◽  
pp. 39-44
Author(s):  
Mahdi Kadkhodazadeh ◽  
Ahmad Reza Ebadian ◽  
Zahra Alizadeh Tabari ◽  
Reza Amid ◽  
Anahita Moscowchi
Keyword(s):  
Chronic Periodontitis ◽  
Cross Sectional Study ◽  
Natural Resistance ◽  
Hybridization Technique ◽  
Periodontal Pathogens ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Macrophage Protein ◽  
Iranian Patients

Background. This study aimed to assess the association of haptoglobin (HP) and natural resistance-associated macrophage protein 1 (NRAMP1) alleles with the presence of heme-consuming periodontal pathogens in a group of Iranian patients with chronic periodontitis and peri-implantitis. Methods. This cross-sectional study evaluated 69 eligible chronic periodontitis and peri-implantitis patients selected from Shahid Beheshti Dental School. The periodontally diseased individuals had at least three teeth with clinical attachment loss of ≥3 mm and a probing pocket depth (PPD) of ≥3 mm in at least two quadrants. Peri-implant PPD of at least one site was ≥5 mm with or without suppuration and bleeding on probing. A plaque index of >20% and a radiographic crestal bone loss was present in at least one site around the implant. The paper point method was used for sampling from the deepest periodontal/peri-implant pocket of each tooth or implant for the DNA checkerboard hybridization technique. Statistical analyses were performed with PASW Statistics 18.0. The variables were presented as absolute and relative frequencies (%). Results. An Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) score of 1–2 was 5.8 times more frequent in HP 2, rs1723540 G, and rs2276631 G alleles. A Porphyromonas gingivalis (P. gingivalis) score of 1–2 was 4.8 times more common in the subjects carrying HP 2, rs1723540 G, and rs2276631 G alleles compared with HP 1, rs1723540 A, and rs2276631 A alleles. Conclusion. Within the limitations of this study, it seems that there was a relationship between HP and NRAMP1 allele frequencies and the presence of heme-consuming periodontal pathogens in the Iranian patients with chronic periodontitis and peri-implantitis evaluated in the present study.

scholarly journals Periodontal P eriodontal Parameters and Anti-Car ameters and Anti-Cardiolipin Antibodies F diolipin Antibodies Following ollowing Periodontal Ther eriodontal Therapy in Chr y in Chronic Periodontitis eriodontitis

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Wan Majdiah Wan Mohamad ◽  
◽  
Noor Rashidah Saad ◽  
Siti Lailatul Akmar Zainuddin ◽  
Haslina Taib ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Periodontal Therapy ◽  
Periodontal Pocket ◽  
Membrane Phospholipids ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Blood Samples ◽  
Periodontal Infection ◽  
Significant Difference

Anti-cardiolipin (anti-CL) antibodies are autoantibodies which are directed against cell membrane phospholipids. A significant number of periodontitis patients showed positive for anti-CL antibody. Objective: This study aimed to determine the periodontal parameters and anti-CL antibodies levels before and after non-surgical periodontal therapy in chronic periodontitis. Methods: This cross-sectional study had been carried out at Periodontal Clinic, Hospital Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia. Thirty five chronic periodontitis (CP) and 39 non-periodontitis (NP) patients underwent clinical periodontal examination at baseline. Plaque index (PI), gingival index (GI), periodontal pocket depth (PPD), and clinical attachment loss (CAL) were measured. Scaling and polishing was performed and blood samples were taken for IgG and IgM anti-CL antibodies analysis. Re-evaluation was performed four weeks after initial therapy. CP patients were re-examined, all periodontal parameters were recorded and blood samples were taken for reassessment of IgG and IgM anti-CL antibodies. Results: Significant difference means of PI (p=0.001), GI (p=0.000), PPD (p=0.000) and, CAL (p=0.000) were found between CP and NP groups. All periodontal parameters were significantly reduced (p≤0.05) after four weeks of therapy. The mean levels of IgG and IgM anti-CL antibodies at baseline were significantly higher in CP than NP group (IgG=4.46 vs 3.22, p=0.002; IgM=3.28 vs 2.57, p=0.019). No significant difference of the median levels of IgG (p=0.82) and IgM anti-CL antibodies (p=0.35) following therapy. Conclusion: All periodontal parameters were significantly reduced following periodontal therapy. Higher level of Anti-CL antibodies in CP indicates stimulation of autoantibodies production by periodontal infection. Nonetheless no significant changes of this anti-CL antibodies levels despite significant reduction of the clinical parameters after periodontal therapy

Optimization of the terms of supportive periodontal therapy using photoactivated disinfection

2019 ◽  
Vol 24 (2) ◽  
pp. 121-126 ◽  
Author(s):  
V. G. Atrushkevich ◽  
L. Yu. Orekhova ◽  
O. O. Yanushevich ◽  
E. Yu. Sokolova ◽  
E. S. Loboda
Keyword(s):  
Chronic Periodontitis ◽  
Adjunctive Treatment ◽  
Control Group ◽  
Clinical Parameters ◽  
Ozone Therapy ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Supportive Periodontal Therapy ◽  
Tannerella Forsythensis ◽  
Observation Period

Relevance: to indentify if periodontal treatment which is presented by photoactivated disinfection (PAD) adjunctively to scaling and root planing (SRP) yield better outcomes than ozone therapy as an adjunct to SRP in periodontitis.Materials and methods: we examined 57 (mean age 49,3 ± 1,02) patients with chronic periodontitis, divided into groups, SRP + PAD, SRP + ozone therapy and SRP alone. Subgingival plaque samples were subjected to DNA extraction and real time PCR amplifcation for detection Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Tannerella forsythensis (Tf), Treponema denticola (Td), Aggregatibacter actinomycetemcomitans (Aa). The amount of periodontopathogens and clinical parameters including plaque index, clinical attachment loss, pocket depth, bleeding on probing were measured at baseline, after 40, 90 and 180 days.Results: the results in groups of PAD+SRP and ozone therapy+SRP showed an improvement in all clinical parameters PI, BOP, PD, CAL and the quantity of Pg, Td and Tf compared to the control group during an observation period.Conclusion: the results showed additional benefts from PAD and ozone therapy as an adjunctive treatment to SRP for patients of chronic periodontitis.

scholarly journals Prevalence of Aggregatibacter actinomycetemcomitans and Periodontal Findings among 14 to 15-Year Old Danish Adolescents: A Descriptive Cross-Sectional Study

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1054
Author(s):  
Anne Birkeholm Jensen ◽  
Flemming Isidor ◽  
Marianne Lund ◽  
Michael Væth ◽  
Anders Johansson ◽  
...  
Keyword(s):  
Aggregatibacter Actinomycetemcomitans ◽  
Cross Sectional Study ◽  
Northern Europe ◽  
Subgingival Plaque ◽  
Sectional Study ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Plaque Score ◽  
Probing Pocket Depth

Aggregatibacter actinomycetemcomitans (Aa) is a keystone pathogen associated with periodontitis in adolescents. The knowledge on the prevalence of Aa and periodontitis among adolescents in Northern Europe is sparse. A total of 525 14- to 15-year-old adolescents from the municipality of Aarhus, Denmark, underwent a full-mouth clinical examination. Plaque score (PS), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment loss (CAL) were recorded. Subgingival plaque samples (SPS) and stimulated saliva samples (SSS) were collected and analyzed for the presence of JP2 and non-JP2 genotypes of Aa using real-time PCR. A total of 70 (13.3%) individuals were positive for Aa, with 17 found in SPS, 19 in SSS, and 35 in both. The highly leukotoxic JP2 genotype of Aa was not detected. The individuals positive for Aa in both SPS and SSS had poorer periodontal outcomes (PPD and CAL) than individuals without Aa and individuals carrying Aa in either SPS or SSS only. In conclusion, 13% of 14- to 15-year-old Danish adolescents were positive for Aa, and the presence of Aa in both SPS and SSS was associated with poorer periodontal outcomes.

SEVERE CHRONIC PERIODONTITIS AND C-REACTIVE PROTEIN LEVELS

2017 ◽  
Vol 6 (2) ◽  
pp. 1
Author(s):  
Johelle De Santana Passos Soares ◽  
Isaac Suzart Gomes-Filho ◽  
Julita Maria Freitas Coelho ◽  
Simone Seixas da Cruz ◽  
Luís Claúdio Lemos Correia ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Descriptive Analysis ◽  
Cross Sectional Study ◽  
Blood Collection ◽  
C Reactive Protein ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Protein Levels ◽  
Reactive Protein ◽  
Significant Difference

This study aims to analyze the relationship between chronic periodontitis and C-reactive protein (CRP), taking certain associated factors into consideration. A cross-sectional study was conducted on a sample of 75 adults of both sexes. After the participants had been interviewed, they underwent physical and dental examinations and blood collection. CRP levels were evaluated by means of nephelometry. A periodontal clinical examination was conducted by trained examiners and the diagnosis of chronic periodontitis was established when at least 30% of the individual’s teeth presented clinical attachment loss ≥ 5 mm. The analysis procedures consisted of descriptive analysis and linear regression. The results showed that there was no statistically significant difference in CRP levels between the groups with and without periodontitis. The median CRP level in the group with periodontitis was 2.3 mg/l (25-75% interquartile range, IQR = 0.74-5.4) and in the group without periodontitis, 1.8 mg/l (25-75% IQR = 0.79-4.54) (p = 0.417). Log CRP was significantly correlated only with the individual’s body mass index (BMI). The main findings from this study indicate that there is no association between severe chronic periodontitis and CRP, and factors like BMI need to be analyzed carefully in studies on this topic.

scholarly journals Prevalence and extent of chronic periodontitis and its risk factors in a Portuguese subpopulation: a retrospective cross-sectional study and analysis of Clinical Attachment Loss

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5258 ◽  
Author(s):  
Vanessa Machado ◽  
João Botelho ◽  
António Amaral ◽  
Luís Proença ◽  
Ricardo Alves ◽  
...  
Keyword(s):  
Risk Factors ◽  
Chronic Periodontitis ◽  
Dental Treatment ◽  
Cross Sectional Study ◽  
Risk Indicators ◽  
Multivariate Logistic Regression Model ◽  
Sectional Study ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Attachment Loss

Objectives To assess the prevalence and extent of chronic periodontitis, and its risk factors in a Portuguese subpopulation referred to periodontal examination. Methods This retrospective cross-sectional study used a subset of data from patients who sought dental treatment in a university dental clinic in the Lisbon metropolitan area. The sample consisted of 405 individuals (225 females/180 males), aged 20–90 years. All patients underwent a full-mouth periodontal examination and chronic periodontitis was defined as Clinical Attachment Loss (CAL) ≥ 3 mm affecting two or more teeth. Aggressive periodontitis cases were excluded from the analysis. Results Prevalence of chronic periodontitis was 83.5% (95% CI [80.4–86.6%]). For these subjects, CAL ≥ 3 mm affected 86.0% (95% CI [84.7–87.2]) of sites and 83.7% (95% CI [81.7–85.6]) of teeth, respectively. Mean CAL ranged from 3.6 to 4.3 mm, according to age. In the multivariate logistic regression model, smoking (OR = 3.55, 95% CI [1.80–7.02]) and older age (OR = 8.70, 95% CI [3.66–20.69] and OR = 4.85, 95% CI [2.57–9.16]), for 65+ and 45–64 years old, respectively, were identified as risk indicators for CAL ≥ 3 mm. Conclusions This particular Portuguese adult subpopulation had a high prevalence of chronic periodontitis, with severe and generalized clinical attachment loss, and its presence was significantly associated with age and smoking. This data should serve to prepare future detailed epidemiological studies and appropriate public health programs.

scholarly journals Periodontal Disease Status among Adults from South Africa—Prevalence and Effect of Smoking

2019 ◽  
Vol 16 (19) ◽  
pp. 3662 ◽  
Author(s):  
Usuf Chikte ◽  
Carla Cruvinel Pontes ◽  
Innocent Karangwa ◽  
Faheema Kimmie-Dhansay ◽  
Rajiv T. Erasmus ◽  
...  
Keyword(s):  
Periodontal Diseases ◽  
Disease Status ◽  
Epidemiological Data ◽  
Cross Sectional Study ◽  
Serum Cotinine ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Public Health Programs ◽  
Present Cross Sectional Study

Periodontal diseases are among the six most prevalent non-communicable diseases (NCDs) worldwide, constituting a burden for oral and general health. There is a shortage of epidemiological data on periodontal diseases in Africa. The aim of the present cross-sectional study was to present the periodontal status and cotinine levels of a South African population of adults. This study included individuals living in the Belville South area. Bleeding on probing (BOP) and pocket depth were recorded for each tooth, and clinical attachment loss (CAL) was recorded as the highest score per sextant. Cotinine levels were measured in ng/mL. A total of 951 individuals were included. More than one third of all subjects had BOP. Regarding pocket depth, over 50% of the subjects had shallow pockets (4–5 mm), and almost 6% had deep pockets. CAL ≥ 4 mm was present in 40.1% of the subjects. Males presented worse periodontal conditions than females. In total, 52.7% of the participants had serum cotinine levels of ≥15 ng/mL. Cotinine levels had no effect on periodontal variables. Periodontal diseases were highly prevalent, and periodontal conditions were worse in males. Preventive and restorative public health programs are required to improve oral health in this population.

scholarly journals Estimation of Salivary Malondialdehyde Levels in Smokeless Tobacco Chewers with Chronic Periodontitis-A Cross Sectional Clinico Biochemical Study

2021 ◽  
pp. 356-365
Author(s):  
Shivaraj B. Warad ◽  
Jyoti Pattanashetti ◽  
Nagaraj Kalburgi ◽  
Arati Koregol ◽  
Subramaniam Rao
Keyword(s):  
Smokeless Tobacco ◽  
Chronic Periodontitis ◽  
Biochemical Study ◽  
Saliva Sample ◽  
Uv Spectrophotometry ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Periodontal Inflammation ◽  
Salivary Malondialdehyde

Objective: Despite the reported effects of smokeless tobacco (ST) on the periodontium and high prevalence of ST use in rural populations and in males studies on this specific topic are limited. The purpose of this cross-sectional investigation was to measure lipid peroxidation (as an end product of oxidative stress) end product i.e. Malondialdehyde (MDA) in saliva of patients with gingivitis, chronic periodontitis and to assess the influence of smokeless tobacco on Salivary Malondialdehyde (S-MDA). Materials and methods: Total 30 patients with gingivitis, 30 with chronic periodontitis and 30 Smokeless Tobacco Chewers with Chronic Periodontitis and 30 periodontally healthy subjects were included in the study. Plaque Index (PI), Gingival Index (GI), Probing Pocket Depth (PD), and Clinical Attachment Loss (CAL) were recorded followed by stimulated Saliva sample collection. Salivary MDA Levels were assessed by UV Spectrophotometry. Results: There was a statistically significant increase in the salivary MDA levels in gingivitis, chronic periodontitis and in smokeless tobacco chewers with chronic periodontitis when compared with healthy group. Conclusions: Higher salivary MDA levels in gingivitis group, chronic periodontitis, and smokeless tobacco chewers with chronic periodontitis reflects increasedoxygen radical activity during periodontal inflammation.

Sign in / Sign up

Export Citation Format

Share Document