scholarly journals Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils

2021 ◽  
Vol 14 (9) ◽  
pp. 842
Author(s):  
Ngamrayu Ngamdokmai ◽  
Kornkanok Ingkaninan ◽  
Nattiya Chaichamnong ◽  
Krongkarn Chootip ◽  
Nitra Neungchamnong ◽  
...  
Keyword(s):  
Essential Oils ◽  
Dosage Form ◽  
Stability Study ◽  
Stability Evaluation ◽  
Herbal Extracts ◽  
Characterization Methods ◽  
Caffeine Content ◽  
The Stability ◽  
Mixed Oil ◽  
Original Formula

Recently, the herbal compress was successfully developed and applied for cellulite treatment. The aim of this study was to formulate a more convenient dosage form of herbal application from the original formula. In addition, we aimed to characterize and evaluate the stability of the developed dosage form. A gelled emulsion, or an “emgel,” incorporated with 0.1 wt% tea and coffee extracts (1:1 ratio) plus 5 wt% essential oils (mixed oil) was prepared. The caffeine content in the finished product obtained from tea and coffee extracts analyzed by HPLC was 48.1 ± 2.3 µg/g. The bio-active marker monoterpenes of mixed oil characterized by headspace GCMS were camphene 50.8 ± 1.8 µg/mg, camphor 251.0 ± 3.2 µg/mg, 3-carene 46.7 ± 1.8 µg/mg, α-citral 75.0 ± 2.1 µg/mg, β-citral 65.6 ± 1.3 µg/mg, limonene 36.8 ± 6.7 µg/mg, myrcene 53.3 ± 4.5 µg/mg, α-pinene 85.2 ± 0.6 µg/mg, β-pinene 88.4 ± 1.1 µg/mg, and terpinene-4-ol 104.3 ± 2.6 µg/mg. The stability study was carried out over a period of 3 months at 4, 25, and 50 °C. The caffeine content showed no significant changes and passed the acceptance criteria of ≥80% at all tested temperatures. However, monoterpenes showed their stability for only 2 months at 50 °C. Therefore, the shelf-life of the emgel was, consequently, calculated to be 31 months using the Q10 method. Thus, the anti-cellulite emgel was successfully formulated. The characterization methods and stability evaluation for caffeine and monoterpenes in an emgel matrix were also successfully developed and validated.

scholarly journals A Fast and Validated HPLC Method for Simultaneous Determination of Dopamine, Dobutamine, Phentolamine, Furosemide, and Aminophylline in Infusion Samples and Injection Formulations

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Fuchao Chen ◽  
Baoxia Fang ◽  
Sicen Wang
Keyword(s):  
Mobile Phase ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Stability Study ◽  
Precision Data ◽  
Column Temperature ◽  
Ich Guidelines ◽  
Limit Of Quantitation ◽  
The Stability

A simple, fast, and validated HPLC method was developed for the simultaneous quantization of five cardiovascular agents: dopamine (DPM), dobutamine (DBM), phentolamine (PTM), furosemide (FSM), and aminophylline (APL) either in infusion samples or in an injection dosage form. The proposed method was achieved with a 150 mm × 4.6 mm, 5.0 μm C18 column, by using a simple linear gradient. Mobile phase A was buffer (50 mM KH2PO4) and mobile Phase B was acetonitrile at a flow rate of 1.0 mL/min. The column temperature was kept at 30°C, and the injection volume was 20 μL. All analytes were separated simultaneously at a retention time (tr) of 3.93, 5.84, 7.06, 8.76, and 9.67 min for DPM, DBM, PTM, FSM, and APL, respectively, with a total run time of less than 15.0 min. The proposed method was validated according to ICH guidelines with respect to accuracy, precision, linearity, limit of detection, limit of quantitation, and robustness. Linearity was obtained over a concentration range of 12.0–240.0, 12.0–240.0, 20.0–200.0, 6.0–240.0, and 10.0–200.0 μg/mL DPM, DBM, PTM, FSM, and APL, respectively. Interday and intraday accuracy and precision data were recorded in the acceptable limits. The new method has successfully been applied for quantification of all five drugs in their injection dosage form, infusion samples, and for evaluation of the stability of investigated drugs in mixtures for endovenous use. The results of the stability study showed that mixtures of DPM, DBM, PTM, FSM, and APL in 5% glucose or 0.9% sodium chloride injection were stable for 48 hours when stored in polypropylene syringes at 25°C.

scholarly journals Formulation and evaluation of potash alum as deodorant lotion and after shaving astringent as cream and gel

2014 ◽  
Vol 3 (2) ◽  
pp. 228-233 ◽  
Author(s):  
Abdulkarim K. Alzomor ◽  
Ahmad Safe Moharram ◽  
Nahlah Mansour Al Absi
Keyword(s):  
Dosage Form ◽  
Inhibitory Concentration ◽  
Pharmaceutical Journal ◽  
Dosage Forms ◽  
Stability Study ◽  
Dispersion Method ◽  
Mueller Hinton ◽  
Normal Microbiota ◽  
The Stability

Potash alum has different traditional application including deodorant and astringent. In order to wide advancement in development of pharmaceutical and cosmetic industries we preferred to work in this field as well as for improvement of safety and efficacy of traditionally used natural products. The aim of the present study was to formulate deodorant potash alum as lotion dosage form and astringent as cream and gel dosage forms. The activity of potash alum against axillaries normal microbiota was tested in vitro using wells diffusion agar method for different concentrations (0.1% to 9%) on Mueller Hinton (MH) agar and the minimum inhibitory concentration (MIC) was determined as 2% and also subculture was done to determine the activity of potash alum as bacteriostatic. Alum powder has wide traditional usage as astringent to prevent bleeding from minor cuts and for its accelerated healing. Therefore, a more appropriate dosage form was designed in a suitable concentration (2% gel) to elongate its astringent activity by its higher retention time due to highly viscous nature of gel. Using suitable excipients, 2% lotion and gel were prepared by dispersion method while emulsification was used for preparation of the potash alum cream of same strength. The above formulations were evaluated by comparing their pH, viscosity, spreadability, contents uniformity and in vitro diffusion. The stability study was carried out at 25°C for 3 months and at 40°C with 75±5% RH for one month. pH, viscosity, spreadability, extrudability and consistency of the products were evaluated at the end of the studies. The results indicated no change in the formulations demonstrating their stable nature as a dosage form and suitability as a commercial product line.DOI: http://dx.doi.org/10.3329/icpj.v3i2.17512 International Current Pharmaceutical Journal, January 2014, 3(2): 228-233

scholarly journals Application of a Validated Method in the Stability Study of Colistin Sulfate and Methylparaben in a Veterinary Suspension Formulation by High-Performance Liquid Chromatography with a Diode Array Detector

2007 ◽  
Vol 90 (3) ◽  
pp. 706-714 ◽  
Author(s):  
Pilar PÉrez-Lozano ◽  
Encarna GarcÍa-Montoya ◽  
Anna Orriols ◽  
Montse MiÑarro ◽  
Josep Ramon TicÓ ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Dosage Form ◽  
Stability Study ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Colistin Sulfate ◽  
The Stability

Abstract A methodology following International Cooperation on Harmonization for Veterinary Products (VICH) guidelines for the stability evaluation of colistin sulfate in a nonaqueous suspension pharmaceutical dosage form for veterinary use (via their drinking water) is described. This method monitors the percentage of colistin sulfate during the stability study of the preparation in drinking water and establishes the shelf life of the final product by a new high-performance liquid chromatography method which was developed and validated for the simultaneous determination of colistin sulfate [colistin A (Polymixin E1) and colistin B (Polymixin E2)] and methylparaben (Nipagin<sup/>) using a diode array detector (DAD). The method uses a Kromasil C18 column and isocratic elution. The mobile phase consisted of an acetonitrilesodium sulfate anhydrous solution (25 + 75) pumped at a flow rate of 1.5 mL/min. The DAD was set at 215 nm. The validation study was carried out according to the VICH guidelines in order to prove that the new analytical method meets the reliability characteristics, which include the fundamental criteria for validation: selectivity, linearity, precision, accuracy, and sensitivity. The method was applied during the quality control or stability studies of the suspension dosage form in order to quantify the drug (colistin) and preservative, and proved to be suitable for rapid and reliable quality control.

Determination of Required Hydrophile-Lipophile Balance Value of Modified Coconut Oil

2014 ◽  
Vol 1060 ◽  
pp. 172-175
Author(s):  
Sukannika Tubtimsri ◽  
Chutima Limmatvapirat ◽  
Pornsak Sriamornsak ◽  
Sontaya Limmatvapirat
Keyword(s):  
Dosage Form ◽  
Coconut Oil ◽  
Stability Study ◽  
Temperature Cycling ◽  
Minimal Size ◽  
Accelerated Stability ◽  
The Stability ◽  
Key Parameter ◽  
Emulsions Stability

Hydrophile–lipophile balance (HLB) is a key parameter for formulation of emulsions. Recently, modified coconut oil (MCO) was successfully developed as antimicrobial oil and was attempted to use as active ingredient in emulsions. Therefore, the aim of this study was to determine the required HLB of MCO. The emulsions was produced with different ratios of emulsifier mixtures of Tween® 80 and Span® 80 , giving HLB from 4.3 to 15.0 and was investigated in respect of their emulsions stability. The result showed that an emulsion with HLB value of 12 demonstrated the most stable in terms of smallest droplet size and minimal size distribution. The stability was also later confirmed by centrifugation and temperature cycling. The emulsions which possessed HLB values closed to required HLB demonstrated minimal creaming and cracking upon accelerated stability study. In conclusion, this study could provide the guideline for formulation of the stable MCO emulsions which may be further developed into suitable dosage form.

scholarly journals 3D sub-nanometer analysis of glucose in an aqueous solution by cryo-atom probe tomography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
T. M. Schwarz ◽  
C. A. Dietrich ◽  
J. Ott ◽  
E. M. Weikum ◽  
R. Lawitzki ◽  
...  
Keyword(s):  
Atom Probe Tomography ◽  
Density Functional ◽  
Chemical Characterization ◽  
Analytical Techniques ◽  
Atom Probe ◽  
Characterization Methods ◽  
Signal Deconvolution ◽  
The Stability ◽  
Frozen Solutions ◽  
3D Volume

AbstractAtom Probe Tomography (APT) is currently a well-established technique to analyse the composition of solid materials including metals, semiconductors and ceramics with up to near-atomic resolution. Using an aqueous glucose solution, we now extended the technique to frozen solutions. While the mass signals of the common glucose fragments CxHy and CxOyHz overlap with (H2O)nH from water, we achieved stoichiometrically correct values via signal deconvolution. Density functional theory (DFT) calculations were performed to investigate the stability of the detected pyranose fragments. This paper demonstrates APT’s capabilities to achieve sub-nanometre resolution in tracing whole glucose molecules in a frozen solution by using cryogenic workflows. We use a solution of defined concentration to investigate the chemical resolution capabilities as a step toward the measurement of biological molecules. Due to the evaporation of nearly intact glucose molecules, their position within the measured 3D volume of the solution can be determined with sub-nanometre resolution. Our analyses take analytical techniques to a new level, since chemical characterization methods for cryogenically-frozen solutions or biological materials are limited.

scholarly journals Clinical Application and Efficacy of Silver Drug in Ophthalmology: A Literature Review and New Formulation of EYE Drops with Drug Silver (I) Complex of Metronidazole with Improved Dosage Form

Biomedicines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 210
Author(s):  
Arleta Waszczykowska ◽  
Dominik Żyro ◽  
Justyn Ochocki ◽  
Piotr Jurowski
Keyword(s):  
Dosage Form ◽  
Complex Analysis ◽  
Bacterial Resistance ◽  
Eye Drops ◽  
Ophthalmic Diseases ◽  
The Stability ◽  
New Formulation ◽  
Additional Therapy ◽  
Antibacterial Potential

The use of silver preparations in medicine is becoming increasingly popular. The basic aim of this evaluation was to review the literature on the clinical (in vivo) and antibacterial potential of silver preparations in ophthalmic diseases. The second goal was to summarize the results of experimental research on the use of silver preparations in ophthalmology. The third objective was to present a method for stabilizing eye drops containing silver (I) complex. Analysis of the pH stability of the silver (I) complex with metronidazole in the prepared dosage form (eye drops) was carried out. Most silver preparations are clinically used for topical application. Few experimental results indicate the usefulness of intraocular or systemic administration of silver (I) preparations as an alternative or additional therapy in infectious and angiogenic eye diseases. The development of a new formulation increases the stability of the dosage form. New forms of silver (I) products will certainly find application in the treatment of many ophthalmic diseases. One of the most important features of the silver (I) complex is its capacity to break down bacterial resistance. The new eye drops formula can significantly improve comfort of use. Due to their chemical nature, silver (I) compounds are difficult to stabilize, especially in the finished dosage form.

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