scholarly journals In Vitro Root Induction from Argan (Argania spinosa (L.) Skeels) Adventitious Shoots: Influence of Ammonium Nitrate, Auxins, Silver Nitrate and Putrescine, and Evaluation of Plantlet Acclimatization

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1062
Author(s):  
Ilham Amghar ◽  
Mohammed Ibriz ◽  
Maha Ibrahimi ◽  
Abdelaali Boudra ◽  
Fatima Gaboun ◽  
...  
Keyword(s):  
Silver Nitrate ◽  
Ammonium Nitrate ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Adventitious Shoots ◽  
Naphthalene Acetic Acid ◽  
High Survival ◽  
Root Induction ◽  
Argania Spinosa

Argania spinosa (L.) Skeels is an endangered plant species endemic to Morocco. In recent years, attempts to develop in vitro regeneration systems for this species were made. However, rooting and acclimatization of in vitro plants have been a bottleneck for successful propagation. In the present study, the effects of different concentrations of auxins, putrescine, silver nitrate (AgNO3) and ammonium nitrate on the in vitro rooting of adventitious shoots of two argan genotypes “Mejji” and “R’zwa”, were evaluated. The highest rooting percentages (86.6% in “Mejji” and 84.4% in “R’zwa”) were observed on Murashige and Skoog (MS) medium modified by reducing the ammonium nitrate concentration and supplemented with 1.5 mg L−1 indole-3-butyric acid (IBA), 0.5 mg L−1 1-naphthalene acetic acid (NAA), 2 mg L−1 AgNO3 and 160 mg L−1 putrescine. This medium resulted in the development of a good root system after only 10 days of culture. Plantlet acclimatization was carried out using different substrate mixtures, and high survival rates (100%) were observed when the substrate contained either peat alone or a sand–peat mixture (1:1, w/w). The high percentages of rooting and acclimatization reported in the present study are of high importance for rapid and large-scale propagation of this endangered species.

scholarly journals Phytohormones Influence on the In-Vitro Regeneration of Saccharum Officinarum L. from Apical Meristem Culture

2014 ◽  
Vol 5 (2) ◽  
pp. 85 ◽  
Author(s):  
Ejiroghene Felix Lawyer ◽  
Z. O. Jamaleddine ◽  
P. T. Lyam ◽  
I. T. Borokini ◽  
A. A. Adedeji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Saccharum Officinarum ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Indole Butyric Acid

Growth regulators especially auxins and cytokinins are critical for plant in-vitro regeneration. The effect of these plant growth regulators on in-vitro propagation of Saccharum officinarum L (Sugarcane) was investigated. In vitro response of two different varieties of sugarcane (NCS 005 and NCS 008) to Plant Growth Regulators was obtained in this study. Formation of buds was obtained on shoot apical meristem when cultured on MS (Murashige and Skoog) medium supplemented with 0.1mg/l BAP (6-Benzylaminopurine). After two weeks of initiation, regenerated meristem was inoculated into MS (Murashige and Skoog) fortified with different concentrations and combination of cytokinins. Shoot multiplication was optimal on 0.5mg/l BAP + 0.25 mg/l Kin(Kinetin) for NCS 005 variety while for NCS 008 variety, no significant (P≥0.05) difference was observed between 1.5mg/l BAP and 1.5mg/l BAP +0.5mg/l Kin. The best root induction for in vitro derived shoots was obtained on 1.0 mg/l NAA (Naphthalene acetic acid) and 2.0 mg/l IBA( Indole butyric acid) for both varieties of sugarcane within ten days of culture transfer. Successfully established plantlets showed excellent growth response when weaned under regulated green house conditions.

scholarly journals A Novel Method for Efficient Micropropagation of Radermachera xylocarpa (Roxb.) K. Schum., a Rare Medicinally Important Forest Species

2013 ◽  
Vol 23 (1) ◽  
Author(s):  
Manjary Sathe ◽  
Megha Vibhute ◽  
Monica Jain ◽  
Pankaj Srivastav
Keyword(s):  
Tree Species ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Forest Tree ◽  
Root Induction ◽  
Mass Propagation ◽  
Forest Tree Species ◽  
Bud Induction ◽  
Indigenous Forest

Radermachera xylocarpa (Roxb.) K. Schum. is a rare indigenous forest tree species which is utilized for its wood and medicinal properties. Due to its overexploitation and specific habitat requirements the species is restricted to limited areas. In vitro mass propagation of tree species faces various challenges and no such efforts have yet been taken in propagation of this useful plant using these methods.  In order to overcome the hurdles and understanding an urgent need of its conservation and mass propagation present authors attempt to develop a simple effective tissue culture protocol for regeneration of R. xylocarpa. Nodal explants were cultured on MS supplemented with various concentrations of cytokinins and auxins.  Among different cytokinins, maximum bud induction and proliferation was obtained in media supplemented with Kn along with IBA and for effective root induction which is tough to obtain in tree species, 100% rooting was achieved in cultures with increasing concentrations of IBA. Field survival is a major challenge with regenerated plants of forest tree species. We report here for the first time 100% survival of plants in soil by carefully standardizing the period of hardening and acclimatization procedures. A novel and effective in vitro regeneration protocol of R. xylocarpa has been successfully standardized which can be adopted for large scale propagation, reforestation and conservation of rare Radermachera xylocarpa of medicinal importance.Plant Tissue Cult. & Biotech. 23(1): 21?29, 2013 (June)DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15556

In vitro propagation of Camellia fascicularis: a plant species with extremely small populations

2020 ◽  
Vol 100 (2) ◽  
pp. 202-208
Author(s):  
Mengting Wang ◽  
Guiliang Zhang ◽  
Peiyao Xin ◽  
Yun Liu ◽  
Bin Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Germplasm Conservation ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium

Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary bud per nodal stem was obtained from C. fascicularis cultured on Murashige & Skoog (MS) medium containing 0.1 mg L−1 indole-3-acetic acid (IAA) combined with 1.0 mg L−1 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to modified woody plant medium (WPM) supplemented with 3.0 mg L−1 BA in combination with 0.3 mg L−1 IAA for multiplication, thereby resulting in a high shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on one-half MS medium supplemented with 2.0 mg L−1 indole-3-butyric acid (IBA) in combination with 0.3 mg L−1 α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing rooting of nodal stems was 1.0 mg L−1 IBA in combination with 2.0 mg L−1 NAA, which resulted in 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.

scholarly journals Effect of Plant Growth Regulators (BA, KIN and NAA) on In vitro Propagation of Papaya (Carica papaya)

2020 ◽  
pp. 15-23
Author(s):  
Nahida Hasan ◽  
Humayra Huq ◽  
Fahima Khatun ◽  
Shamim Ara Sumi
Keyword(s):  
In Vitro Propagation ◽  
Carica Papaya ◽  
In Vitro Regeneration ◽  
Combined Treatment ◽  
Survival Rates ◽  
Tween 20 ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Breeding Programs

The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207 from the period of September 2017 to June 2018. This research aims to study the effect of Benzyladenine (BA), Kinetin (KIN) and Naphthalene acetic acid (NAA) either in combination or alone on In vitro propagation of papaya (Carica papaya). The shoot tips of young shoots were used as explant, which was sterilized using freshly prepared 0.1% HgCl2 mixing with few drops of Tween-20, were inoculated in MS media supplemented with 0.1% activated charcoal. The minimum days to shoot induction (10.25) were recorded on MS medium containing 0.5 mg/L BA. The highest shoots (4.5) and length of shoot (5.75 cm) observed in 1.0 mg/L BA. The combined treatment 1.0 mg/L BA+0.75 mg/LKIN gave the highest number of shoots (5.25) and length of shoot (5.78 cm).The minimum days (8.5) to root induction was reported in 2.0 mg/L NAA along with maximum 8.25 roots per plantlet. The highest length of root (6.92 cm) was observed in 2.0 mg/L NAA. In regenerated plantlets, 80% survival rates were observed in growth chamber conditions and 75% in the open atmosphere were achieved. Finally, the in vitro regeneration protocol described herein can potentially be used as a tool in molecular breeding programs for the improvement of different cultivars and genotypes of papaya.

scholarly journals In vitro Regeneration of Saussurea heteromalla (D. Don.) Hand-Mazz Using Auxins and Current Status in Galiyat Abbottabad

2020 ◽  
Vol 10 (5) ◽  
pp. 1-7
Author(s):  
H. Mehreen ◽  
J. Zafar ◽  
G. Zishan
Keyword(s):  
In Vitro Regeneration ◽  
In Vitro Rooting ◽  
Current Status ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Indole Butyric Acid ◽  
Half Strength ◽  
Emergence Percentage

Current Status of Saussurea heteromalla was investigated in Galiyat areas of District Abbottabad, viz., Jahaffar, Seri, Beeran Gali, Banj, Haryala, Daryala Gali, Sarbhanna, Barriyan, Akhreela and Broangiala. Saussurea heteromalla was found common in Seri, Sarbhanna and Barriyan; endangered in Haryala, Jahaffar, Banj, Daryala Gali and Beeran Gali and absent in Akhreela and Broangiala. In vitro regeneration of Saussurea heteromalla (D. Don.) Hand-Mazz on MS media was conducted and shoots were developed on full strength MS medium supplemented with 1 mgL-1 GA3. The developed shoots were transferred for root induction to half strength MS medium fortified with various concentrations of Indole butyric acid (IBA) and α-Naphthalene acetic acid (NAA) i.e. T1 (control), T2 (2 mgL-1 IBA), T3 (3 mgL-1 IBA), T4 (4 mgL-1 IBA), T5 (1 mgL-1 NAA), T6 (2 mgL-1 NAA) and T7 (3 mgL-1 NAA). Maximum mean shoot length (6.3 cm), mean number of leaves (7), mean number of nodes (5.25); highest root emergence percentage (71%), means root length (1.5 cm), mean number of roots (3) and highest survival rate (100%) was recorded in treatment T4. However, treatments T5 and T6 also seem to be effective for in vitro rooting of Saussurea heteromalla. Treatments T2, T3 and T7 showed minimum root growth. It was concluded that IBA at higher concentration is more effective for in vitro rooting and better shoot growth of Saussurea heteromalla whereas NAA also initiate rooting but at lower concentration.

scholarly journals Comparison of Phytochemical and Antioxidant Activities in Micropropagated and Seed-derived Salvia miltiorrhiza Plants

HortScience ◽  
2018 ◽  
Vol 53 (7) ◽  
pp. 1038-1044 ◽  
Author(s):  
Bo-Ling Liu ◽  
Zhi-Bin Fan ◽  
Ze-Qun Liu ◽  
Xun-Hong Qiu ◽  
Yan-Hong Jiang
Keyword(s):  
Seed Germination ◽  
Antioxidant Activities ◽  
Salvia Miltiorrhiza ◽  
In Vitro Regeneration ◽  
Antioxidant Properties ◽  
Adventitious Shoots ◽  
Chemical Properties ◽  
Naphthalene Acetic Acid ◽  
Ms Medium

Salvia miltiorrhiza (commonly known in China as Danshen) is widely used in traditional Chinese medicine, and it is applied in the treatment of many diseases, particularly cardiovascular disease. Commercial propagation of Danshen is carried out either through seed germination or in vitro regeneration (micropropagation). However, it is not clear if the different propagation methods affect the chemical properties of the derived plants. In the present study, we first established a highly efficient tissue culture system for Danshen propagation. The addition of 1.0 mg·L−1 6-benzyladenine (BA) and 0.1 mg·L−1 α-naphthalene acetic acid (NAA) to Murashige and Skoog (MS) medium was optimal for inducing adventitious shoots; the highest rate of rooting was recorded on MS medium with 0.2 mg·L−1 NAA, on which the survival rate of transplanted plantlets was 95%. Next, we assessed antioxidant properties in the different tissues of plants of the same age, derived from micropropagation or seed germination, and measured tanshinone, total phenol, and total flavonoid contents. Our results showed that tissues of micropropagated plantlets had higher antioxidant activities than tissues of seed-derived plantlets; the micropropagated plantlets also had higher tanshinone contents in their roots. Thus, a rapid and efficient micropropagation system was established for Danshen, and it can be used for cultivating this plant to obtain therapeutic compounds.

In vitro regeneration of Moringa oleifera Lam.: A medicinal tree of family Moringaceae

2019 ◽  
Vol 79 (3) ◽  
Author(s):  
Suresh . Chand ◽  
Ashu . Pandey ◽  
Oshin . Verma
Keyword(s):  
Somatic Embryogenesis ◽  
Moringa Oleifera ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Apices ◽  
Moringa Oleifera Lam

A protocol is developed for somatic embryogenesis and organogenesis from nodal segments and shoot-apices of Moringa oleifera Lam. Callusing and multiple shoots induction occurred in nodal segments (0.5-1.0 cm) after 2 weeks of culture on Woody Plant Medium, 1981 (WPM) containing N6-benzyladenine (BA), alone or in combinations with various concentrations of á-naphthalene acetic acid (NAA). The concentration of BA at 4.44 μM was found optimum for multiple shoots formation, resulting in 3.22 ± 0.17 shoots per explants. WPM was enriched by adding certain additives to minimize yellowing and shedding of leaves in proliferated shoots. Maximum roots induction (86.6%) was observed in enriched WPM containing 2.46 μM IBA. Somatic embryos were developed from the calli obtained from nodal segments on enriched WPM supplemented with IBA and BA. Histological studies were carried out for studying the pattern of somatic embryogenesis. Multiple shoots formed from shoot apices (1.0-1.5 cm) cultured on enriched WPM (with or without IBA) after 2 weeks of culture. Root induction occurred simultaneously. The regenerated plantlets were gradually transferred to half strength and later to quarter strength liquid WPM, for 10 days, before transferring to pots. Approximately, 70% survival rate was recorded from in vitro grown plantlets on transfer to pots. Somatic embryogenesis in M. oleifera from nodal explants is reported for the first time in this communication.

scholarly journals In Vitro Regeneration of Castor (Ricinus Communis L.) Using Cotyledon Explants

HortScience ◽  
2008 ◽  
Vol 43 (1) ◽  
pp. 215-219 ◽  
Author(s):  
Yeh-Jin Ahn ◽  
Grace Qianhong Chen
Keyword(s):  
Ricinus Communis ◽  
In Vitro Regeneration ◽  
Adventitious Shoots ◽  
Shoot Elongation ◽  
Induction Medium ◽  
Root Induction ◽  
Oilseed Crop ◽  
Ms Medium ◽  
Cotyledon Explants

An efficient plant regeneration protocol using cotyledon explants was established for castor (Ricinus communis L.), an important oilseed crop. Mature seed-derived cotyledon explants produced adventitious shoots when placed on Murashige and Skoog (MS) medium containing thidiazuron (TDZ). The rate of shoot regeneration was maximal (≈25 shoots per explant) when explants were cultured on shoot induction medium supplemented with 5 μm TDZ and preincubated in the dark for the first 7 days before transferring to the day/night cycle (16/8 h). Only the proximal ends of cotyledon explants produced adventitious shoots, although green calli were observed in cotyledon veins. After 4 weeks in culture, explants with well-developed shoot buds were transferred to MS medium without plant growth regulators for the shoot elongation and development. At ≈4 months after culture initiation, shoots (2 cm in length) were transferred to root induction medium (MS medium supplemented with 5 μm indole-3-butyric acid) where they developed roots in 4 to 6 weeks. Plantlets were transferred to soil and acclimatized to greenhouse conditions. Histological analysis showed the adventitious induction of the shoots originated from the cortical and epidermal cell layers of the cotyledon explants.

In vitro regeneration of Persian poppy (Papaver bracteatum)

Biologia ◽  
2010 ◽  
Vol 65 (4) ◽  
Author(s):  
Sara Rostampour ◽  
Haleh Sohi ◽  
Ali Dehestani
Keyword(s):  
Ascorbic Acid ◽  
Callus Induction ◽  
High Performance ◽  
High Efficiency ◽  
In Vitro Regeneration ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Papaver Bracteatum ◽  
Callus Proliferation

AbstractPersian poppy (Papaver bracteatum Lindl.) is an important commercial source of medicinal opiates and related compounds. In this research, calli were induced from seeds, roots, cotyledons and hypocotyls of P. bracteatum at a high efficiency. The optimized callus induction media consisted of the Murashige and Skoog (MS) basic media supplemented with 1.0 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D), 0.1 mg/L kinetin and 15 mg/L ascorbic acid. The concentrations of 2,4-D and ascorbic acid were found critical to callus induction and proliferation. Subsequent subcultures resulted in excellent callus proliferation. Ascorbic acid at concentration 15 mg/L increased the callus proliferation significantly. Maximum callus growth was achieved when the explants were incubated at 25°C. MS salts at full strength were found inhibitory for callus induction, while ľ MS salts were found to favor callus induction. Shoot regeneration of calli in vitro was achieved on ľ MS medium containing 0.5 mg/L benzylamine purine and 1.0 mg/L naphthalene acetic acid. Analysis of alkaloid extracts from Persian poppy tissues by high-performance liquid chromatography showed that thebaine accumulated in the tissues of plants. The thebaine alkaloid profile of the Persian poppy is a well-defined model to evaluate the potential for metabolic engineering of thebaine production in P. bracteatum.

scholarly journals In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

2016 ◽  
Vol 8 (2) ◽  
pp. 161-163
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Large Scale ◽  
Leaf Disc ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Root Induction ◽  
Survival Percentage ◽  
Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

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