Phytotoxic Activity of p-Cresol, 2-Phenylethanol and 3-Phenyl-1-Propanol, Phenolic Compounds Present in Cistus ladanifer L.

Numerous studies about the leaf exudate of Cistus ladanifer highlight this Mediterranean shrub as an allelopathic species. Despite the very high diversity of secondary metabolites identified in its labdanum, only a few components have been evaluated. p-Cresol, 2-phenylethanol and 3-phenyl-1-propanol are three phenolic components present in the labdanum of C. ladanifer whose role has not been specified to date. The present study, through a static acute toxicity test, analyzed their activity, with respect to Allium cepa and Lactuca sativa. These three separate compounds and the mixture of all of them have a more or less phytotoxic activity depending on the medium, species and concentration tested. When the test is carried out on paper, the three pure allelochemicals and their mixture at 1 mM significantly inhibited the total germination, the germination rate and the development of the Allium cepa and Lactuca sativa seedlings to a lesser extent, but when the test performed in soil, the effects on the size of roots and cotyledons are attenuated. Furthermore, in the two species tested on paper, the joint action of the three compounds at 1 mM shows a significantly greater inhibition of the measured indices than each of the compounds separately.

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Environmental Risk Characterization of an Antiretroviral (ARV) Lamivudine in Ecosystems

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18168358 ◽

2021 ◽

Vol 18 (16) ◽

pp. 8358

Author(s):

Elizabeth Oyinkansola Omotola ◽

Bettina Genthe ◽

Luyanda Ndlela ◽

Olatunde Stephen Olatunji

Keyword(s):

Daphnia Magna ◽

Allium Cepa ◽

Lactuca Sativa ◽

Germination Rate ◽

Survival Rates ◽

Antiretroviral Drugs ◽

Trophic Levels ◽

Adverse Impact ◽

Root Tip ◽

Root Tips

Antiretroviral drugs for the treatment of human immunodeficiency virus (HIV) and other viral infections are among the emerging contaminants considered for ecological risk assessment. These compounds have been reported to be widely distributed in water bodies and other aquatic environments, while data concerning the risk they may pose to unintended non-target species in a different ecosystem (environment) is scanty. In South Africa and other developing countries, lamivudine is one of the common antiretrovirals applied. Despite this, little is known about its environmental impacts as an emerging contaminant. The present study employed a battery of ecotoxicity bioassays to assess the environmental threat lamivudine poses to aquatic fauna and flora. Daphnia magna (filter feeders), the Ames bacterial mutagenicity test, Lactuca sativa (lettuce) germination test, and the Allium cepa root tip assay were conducted, testing lamivudine at two concentrations (10 and 100 µg/L), with environmental relevance. The Daphnia magna toxicity test revealed a statistically significant response (p << 0.05) with a mortality rate of 85% on exposure to 100 µg/L lamivudine in freshwater, which increased to 100% at 48-h exposure. At lower concentrations of 10 µg/L lamivudine, 90% and 55% survival rates were observed at 24 h and 48 h, respectively. No potential mutagenic effects were observed from the Ames test at both concentrations of lamivudine. Allium cepa bioassays revealed a noticeable adverse impact on the root lengths on exposure to 100 µg/L lamivudine. This impact was further investigated through microscopic examination, revealing some chromosomal aberration in the exposed Allium cepa root tips. The Lactuca sativa bioassay showed a slight adverse impact on both the germination rate of the seeds and their respective hypocotyl lengths compared to the control. Overall, this indicates that lamivudine poses an ecological health risk at different trophic levels, to both flora and fauna, at concentrations previously found in the environment.

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Metabolites from Withania aristata with Potential Phytotoxic Activity

Natural Product Communications ◽

10.1177/1934578x1000500712 ◽

2010 ◽

Vol 5 (7) ◽

pp. 1934578X1000500

Author(s):

Gabriel G. Llanos ◽

Rosa M. Varela ◽

Ignacio A. Jiménez ◽

José M. G. Molinillo ◽

Francisco A. Macías ◽

...

Keyword(s):

Allium Cepa ◽

Lactuca Sativa ◽

Lepidium Sativum ◽

Wheat Coleoptile ◽

Lycopersicum Esculentum ◽

Target Species ◽

Phytotoxic Activity ◽

First Time

A series of apocarotenoids (1-8) and one carotenoid (9) were isolated from the leaves of Withania aristata. In addition, the tetraacetylated apocarotenoid glucosides 10-12 were obtained by acetylation, with derivative 9-hydroxymegastigma-4,6 E-dien-3-one 9- O-β-D-glucopyranoside tetraacetate (10) being described for the first time. The structures have been determined by spectroscopic and spectrometric means, mainly NMR and ESIMS, and comparison with data reported in the literature. These metabolites were evaluated on a systematic phytotoxicity assay using the etiolated wheat coleoptile bioassay. Compounds 1-3, 9 and 12 were further assayed for their phytotoxicity on the target species Lepidium sativum, Lactuca sativa, Lycopersicum esculentum and Allium cepa. Among the assayed compounds, lutein (9) showed the most significant values for phytotoxicity, followed by the non-glycosylated apocarotenoids (6 S, 9 R)-vomifoliol (1) and 9-hydroxymegastigma-4,6 E-dien-3-one (2).

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Influence of Heat on Seed Germination of Cistus Laurifolius and Cistus Ladanifer

International Journal of Wildland Fire ◽

10.1071/wf9920015 ◽

1992 ◽

Vol 2 (1) ◽

pp. 15 ◽

Cited By ~ 47

Author(s):

L Valbuena ◽

R Tarrega ◽

E Luis

Keyword(s):

Seed Germination ◽

High Temperatures ◽

Exposure Time ◽

Germination Rate ◽

Heat Exposure ◽

Cistus Ladanifer ◽

Different Temperatures ◽

Petri Dishes ◽

Germinated Seeds

The influence of high temperatures on germination of Cistus laurifolius and Cistus ladanifer seeds was analyzed. Seeds were subjected to different temperatures for different times, afterwards they were sowed in plastic petri dishes and monitored for germinated seeds over two months.The germination rate observed in Cistus ldanifer was greater than in Cistus laurifolius. In both species, heat increased germination percentages. For Cistus laurifolius higher temperatures or longer exposure times were needed. Germination percentages of Cistus ladanifer were lower when heat exposure time was 15 minutes.It must be emphasized that germination occurred when seeds were not treated, while seeds exposed to 150�C for 5 minutes or more did not germinate.

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Índices de calidad ambiental de aguas del Arroyo Caañabe mediante tests microbiológicos y ecotoxicológico

Ambiente e Agua - An Interdisciplinary Journal of Applied Science ◽

10.4136/ambi-agua.1703 ◽

2016 ◽

Vol 11 (3) ◽

pp. 548

Author(s):

Tomás Rodrigo López Arias ◽

Virginia Florencia Fernández Peralta ◽

Deidamia Mercedes Franco de Diana ◽

Edgar Fidel Galeano Delgado ◽

Fernando Salvador Alonso Márquez ◽

...

Keyword(s):

Daphnia Magna ◽

Allium Cepa ◽

Danio Rerio ◽

Lactuca Sativa ◽

E Coli ◽

Los Grupos

El Arroyo Caañabe, es un curso de agua que corre a través de los Departamentos Central y Paraguarí (República del Paraguay). Las descargas de origen agropecuario, urbano e industrial contaminan sus aguas. En este trabajo se evalúa la calidad del arroyo mediante sus características fisicoquímicas, ecotoxicológicas, microbiológicas y se la comparan con la legislación vigente. Se colectaron muestras en los meses de Julio y Setiembre del año 2014. Se estudiaron tres sitios denominados S1, ubicado en aguas arriba de la Ciudad de Carapegua; S2 en la intersección del arroyo con la Ruta 1, y S3, en la zona límite de las ciudades de Carapegua y Nueva Italia. Se realizaron ensayos de toxicidad aguda con Daphnia magna, Lactuca sativa, y alevines de Danio rerio; además de ensayos crónicos en Tetradesmus wisconsinenesis, D. rerio y Allium cepa. Se evaluaron los grupos y especies microbianos siguientes: aerobios mesófilos, enterobacterias, coliformes totales, coliformes fecales, E. coli, Pseudomona aeruginosa, mohos y levaduras; además se determinaron índices de calidad y de contaminación. Los resultados indican que las aguas del arroyo presentaron características de clase II y de clase III según el padrón establecido por la Secretaría del Ambiente del Paraguay. El índice de calidad de agua (ICA) arrojó valores comprendidos entre 52 y 62 lo que otorga la clasificación de “regular”; mientras que el índice de contaminación trófica (ICOTRO) presentó valores entre 0,12 y 0.26, indicando “eutrofización”. Los recuentos de coliformes fueron superiores en el segundo muestreo, aunque dentro lo establecido por la norma. La presencia de P. aeruginosa en los tres puntos constituye un riesgo para la salud. Ensayos ecotoxicológicos agudos mostraron que las aguas presentan escasos efectos letales, no obstante los ensayos crónicos en A. cepa y el test de micronúcleos en D. rerio indican potenciales efectos citotóxicos y genotóxicos de las aguas del Arroyo Caañabe.

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Potencial fitotóxico de Dicranopteris flexuosa (Schrad.) Underw. (Gleicheniaceae)

Acta Botanica Brasilica ◽

10.1590/s0102-33062011000100012 ◽

2011 ◽

Vol 25 (1) ◽

pp. 95-104 ◽

Cited By ~ 2

Author(s):

Valerí Schmidt da Silva ◽

Ana Carina da Silva Cândido ◽

Caroline Muller ◽

Valdemir Antônio Laura ◽

Odival Faccenda ◽

...

Keyword(s):

Triticum Aestivum ◽

Lycopersicon Esculentum ◽

Allium Cepa ◽

Lactuca Sativa ◽

Triticum Aestivum L ◽

Lactuca Sativa L ◽

Allium Cepa L

O objetivo deste trabalho foi determinar o potencial fitotóxico do extrato etanólico bruto e das frações semipurificadas de Dicranopteris flexuosa por meio de bioensaios de germinação e crescimento de Lactuca sativa L. (alface), Lycopersicon esculentum L. (tomate), Allium cepa L. (cebola) e Triticum aestivum L. (trigo) em laboratório e casa de vegetação e quantificar o teor total de fenóis e flavonóides dos extratos e frações. Nos bioensaios realizados em laboratório foram utilizadas quatro concentrações (0, 250, 500, 1000 mg L-1), com quatro repetições de 50 sementes. A análise dos resultados indica redução da velocidade e/ou inibição da germinação, estímulo do crescimento da raiz das eudicotiledôneas e inibição da raiz adventícia das monocotiledôneas estudadas. Nos bioensaios realizados em casa de vegetação foram utilizadas as mesmas concentrações dos bioensaios em laboratório, com oito repetições de cinco sementes por vaso. A análise dos resultados indica que o comprimento da raiz foi afetado pelo extrato etanólico bruto, ocorrendo estímulo em tomate e inibição em cebola e trigo. A produção de massa seca da parte aérea foi estimulada na menor concentração em alface e trigo. A fração acetato de etila foi a que apresentou os maiores teores de fenóis e flavonóides totais. Embora os resultados sejam preliminares, observa-se que o extrato etanólico e as frações semipurificadas de D. flexuosa também contêm substâncias que interferem no crescimento das plântulas de alface, tomate, cebola e trigo.

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Evolution and Expansion of Flowering Plants

The Paleontological Society Papers ◽

10.1017/s1089332600000772 ◽

2000 ◽

Vol 6 ◽

pp. 209-232 ◽

Cited By ~ 5

Author(s):

Scott L. Wing

Keyword(s):

Large Scale ◽

Terrestrial Ecosystems ◽

Taxonomic Diversity ◽

Climatic Conditions ◽

Flowering Plants ◽

Terrestrial Vegetation ◽

Earth History ◽

Very High ◽

High Diversity ◽

Environmental Disruption

Flowering plants are a classic example of a group arising late in Earth history and yet achieving very high diversity, abundance, and ecological and morphological variety in a great array of environments and climatic conditions on all continents. Thus, the success of flowering plants raises basic questions about how new lineages become inserted into existing terrestrial ecosystems. To what degree did flowering plants replace older lineages competitively, and to what extent did their expansion depend on large-scale environmental disruption or extinction of older groups? Is the higher taxonomic diversity of flowering plants a consequence of higher rates of speciation, lower rates of extinction, or both? Have flowering plants expanded the total area and range of habitats occupied by terrestrial vegetation? What were the effects of the diversification and spread of flowering plants on the structure of habitats and the types of resources available to terrestrial heterotrophs?

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Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes

Revista Colombiana de Ciencias Pecuarias ◽

10.17533/udea.rccp.v32n2a04 ◽

2019 ◽

Vol 32 (2) ◽

pp. 107-116 ◽

Cited By ~ 1

Author(s):

Meysam Ahmadzadeh ◽

Farzad Rashidi ◽

Hamed Amirpour Najafabadi ◽

Amir Jaferian ◽

Shahin Eghbalsaied

Keyword(s):

Body Weight ◽

Milk Production ◽

Genetic Information ◽

Water Buffaloes ◽

Khuzestan Province ◽

Strategic Plans ◽

Ghr Gene ◽

Pcr Rflp ◽

Very High ◽

High Diversity

Background: Genetic information is necessary to devise strategic plans aimed to improve the genetic merit of buffalos. Objective: To assess the effect of genetic polymorphisms in GH, Pit-1, GHR, GHRHR, and KCN3 genes on milk production and body weight of Khuzestan water buffaloes. Methods: Blood samples were collected from 60 buffaloes from the Khuzestan province, Iran. Using the PCR-RFLP technique, the amplified and digested fragments of GH/AluI, GHR/AluI, GHRHR/ HaeIII, Pit1/HinfI, and KCN3/HindIII were genotyped. Results: All animals were monomorphic for GHRHR. The frequency of mutant alleles for GH, GHR, KCN3, and Pit1 was 47.5, 74.2, 49.2, and 51.7%, respectively. There were significant differences (p<0.0001) in the genotypic frequencies of GH, GHR, and Pit1 between high and low milk-yielding buffaloes. The GH (p=0.0002), GHR (p<0.0001) and Pit1 (p<0.0001) polymorphisms also had significant effects on body weight. Sequencing results revealed the presence of C496A, G495A, G498A and C1501T SNPs in the GH, and G1702T in the GHR gene of Khuzestan buffalos. Conclusion: This study highlights the importance of GH, GHR, and Pit1 on milk production and body weight of Khuzestan buffaloes. The results suggest that devising an integrated breeding plan in Khuzestan water buffalos can considerably benefit from the very high diversity in candidate genes.Keywords: animal breeding, casein, genetic variation, growth hormone, milk production, SNP. ResumenAntecedentes: La información genética es necesaria para diseñar planes estratégicos con el objeto de mejorar el mérito genético de los búfalos. Objetivo: Evaluar el efecto de los polimorfismos genéticos en los genes GH, Pit-1, GHR, GHRHR y KCN3 sobre la producción láctea y peso corporal de búfalos de agua de la provincia de Juzestán, Iran. Métodos: Se recolectaron 60 muestras de sangre de búfalos de la provincia de Juzestán, en Irán. Los fragmentos amplificados y digeridos de GH/AluI, GHR/AluI, GHRHR/HaeIII, Pit1/HinfI y KCN3/HindIII fueron clasificados genotípicamente, utilizando la técnica PCR-RFLP. Resultados: Todos los animales fueron monomórficos para el gen GHRHR. La frecuencia alélica de alelos mutantes para los genes GH, GHR, KCN3 y Pit1 fue 47,5, 74,2, 49,2 y 51,7%, respectivamente. Se encontraron diferencias significativas (p<0,0001) en las frecuencias genotípicas de GH, GHR y Pit1 entre búfalos de alta y baja producción. El efecto del polimorfismo GH (p=0,0002), GHR (p<0,0001) y Pit1 (p<0,0001) también fue significativo para peso corporal. Los resultados de la secuenciación revelaron la presencia de SNPs C496A, G495A, G498A y C1501T en GH, y G1702T en el gen GHR. Conclusiones: Este estudio resalta la importancia de los genes GH, GHR y Pit1 sobre la producción de leche y el peso corporal de búfalos de Juzestán. Los resultados sugieren que la elaboración de un plan de cruzamiento integrado en búfalos de agua de Juzestán puede beneficiarse considerablemente de la gran diversidad de genes candidatos.Palabras clave: caseína, hormona del crecimiento, producción de leche, reproducción animal, SNP, variación genética. ResumoAntecedentes: Determinação informações genéticas é o passo crítico para elaborar planos estratégicos com o objetivo de melhorar o mérito genético dos búfalos. Objetivo: Avaliar o efeito de polimorfismos genéticos nos genes GH, Pit-1, GHR, GHRHR e KCN3 na produção de leite e no peso corporal dos búfalos de água do Cuzistão, Irã. Métodos: Amostras de sangue foram coletadas de 60 búfalos da província de Cuzistão, no Irã. Utilizando a técnica PCR-RFLP, os fragmentos amplificados e digeridos de GH/AluI, GHR/AluI, GHRHR/HaeIII, Pit1/HinfI e KCN3/HindIII foram genotipados. Resultados: Todos os animais eram monomórficos para o gene GHRHR. A freqüência alélica de alelos mutantes para os genes GH, GHR, KCN3 e Pit1 foi 47,5, 74,2, 49,2 e 51,7%, respectivamente. Uma diferença significativa (p<0,0001) foi encontrada nas freqüências genotípicas de os genes GH, GHR e Pit1 entre búfalos de alta e baixa produção. O efeito do polimorfismo GH (p=0,0002), GHR (p<0,0001) e Pit1 (p<0,0001) também foi significativo para o peso corporal. Os resultados da sequenciação revelaram a presença de SNPs C496A, G495A, G498A e C1501T no GH, e G1702T no gene GHR dos buffalos do Cuzistão. Conclusões: Este estudo destacou a importância da GH, GHR e Pit1 na produção de leite e no peso corporal de buffalos do Cuzistão. Os resultados sugerem que a elaboração de um plano de melhoramiento genético integrado em búfalos de água do Cuzistão pode beneficiar consideravelmente da grande diversidade de genes candidatos.Palavras-chave: caseína, hormônio do crescimento, produção de leite, reprodução animal, SNP, variação genética.

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Isolation and Identification of Fusarium spp., the Causal Agents of Onion (Allium cepa) Basal Rot in Northeastern Israel

Biology ◽

10.3390/biology9040069 ◽

2020 ◽

Vol 9 (4) ◽

pp. 69

Author(s):

Ben Kalman ◽

Dekel Abraham ◽

Shaul Graph ◽

Rafael Perl-Treves ◽

Yael Meller Harel ◽

...

Keyword(s):

Allium Cepa ◽

Fusarium Species ◽

Germination Rate ◽

Post Inoculation ◽

Fusarium Spp ◽

Isolation And Identification ◽

Preventive Program ◽

Onion Bulbs ◽

Basal Rot

Over the past decade, there have been accumulating reports from farmers and field extension personnel on the increasing incidence and spread of onion (Allium cepa) bulb basal rot in northern Israel. The disease is caused mainly by Fusarium species. Rotting onion bulbs were sampled from fields in the Golan Heights in northeastern Israel during the summers of 2017 and 2018. Tissue from the sampled onion bulbs was used for the isolation and identification of the infecting fungal species using colony and microscopic morphology characterization. Final confirmation of the pathogens was performed with PCR amplification and sequencing using fungi-specific and Fusarium species-specific primers. Four Fusarium spp. isolates were identified in onion bulbs samples collected from the contaminated field: F. proliferatum, F. oxysporum f. sp. cepae, and two species less familiar as causative agents of this disease, F. acutatum and F. anthophilium. Phylogenetic analysis revealed that these species subdivided into two populations, a northern group isolated from white (Riverside cv.) onion bulbs, and a southern group isolated from red (565/505 cv.) bulbs. Pathogenicity tests conducted with seedlings and bulbs under moist conditions proved that all species could cause the disease symptoms, but with different degrees of virulence. Inoculating seeds with spore suspensions of the four species, in vitro, significantly reduced seedlings’ germination rate, hypocotyl elongation, and fresh biomass. Mature onion bulbs infected with the fungal isolates produced typical rot symptoms 14 days post-inoculation, and the fungus from each infected bulb was re-isolated and identified to satisfy Koch’s postulates. The onion bulb assay also reflected the degree of sensitivity of different onion cultivars to the disease. This work is the first confirmed report of the direct and primary cause of Fusarium onion basal rot disease in northeastern Israel. These findings are a necessary step towards uncovering the mycoflora of the diseased onion plants and developing a preventive program that would reduce the disease damage.

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Increasing our knowledge of Brazilian bamboos: two new species of Chusquea subg. Rettbergia (Bambusoideae, Poaceae)

Phytotaxa ◽

10.11646/phytotaxa.161.3.3 ◽

2014 ◽

Vol 161 (3) ◽

pp. 201 ◽

Cited By ~ 1

Author(s):

Aline Costa Mota ◽

REYJANE PATRÍCIA DE OLIVEIRA ◽

LYNN G. CLARK

Keyword(s):

New Species ◽

Atlantic Forest ◽

Conservation Status ◽

Minas Gerais ◽

Foliage Leaf ◽

Ecological Aspects ◽

Two New Species ◽

Woody Bamboos ◽

Very High ◽

High Diversity

Brazil includes a very high diversity of bamboos, but this diversity is far from completely known. We increase the list of Brazilian woody bamboos by describing two new species of Chusquea, which belong to subgenus Rettbergia. Chusquea ciliatifolia sp. nov. differs from other species of this subgenus by its mottled culms, distinctly ciliate margins of the foliage leaf blades and pilose synflorescences; and C. mirabilis sp. nov. by the presence of branches with sericeous girdles. Both C. ciliatifolia and C. mirabilis occur exclusively in the Atlantic Forest, from the states of Bahia and Minas Gerais, known only from five and three populations, respectively. Comments on morphology, taxonomic relationships, ecological aspects, and conservation status of these new species are provided.

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