Chemical and Biological Profiles of Dendrobium in Two Different Species, Their Hybrid, and Gamma-Irradiated Mutant Lines of the Hybrid Based on LC-QToF MS and Cytotoxicity Analysis

The Dendrobium species (Orchidaceae) has been cultivated as an ornamental plant as well as used in traditional medicines. In this study, the chemical profiles of Dendrobii Herba, used as herbal medicine, Dendrobium in two different species, their hybrid, and the gamma-irradiated mutant lines of the hybrid, were systematically investigated via ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QToF MS). Among the numerous peaks detected, 17 peaks were unambiguously identified. Gigantol (1), (1R,2R)-1,7-hydroxy-2,8-methoxy-2,3-dihydrophenanthrene-4(1H)-one (2), tristin (3), (−)-syringaresinol (4), lusianthridin (5), 2,7-dihydroxy-phenanthrene-1,4-dione (6), densiflorol B (7), denthyrsinin (8), moscatilin (9), lusianthridin dimer (10), batatasin III (11), ephemeranthol A (12), thunalbene (13), dehydroorchinol (14), dendrobine (15), shihunine (16), and 1,5,7-trimethoxy-2-phenanthrenol (17), were detected in Dendrobii Herba, while 1, 2, and 16 were detected in D. candidum, 1, 11, and 16 in D. nobile, and 1, 2, and 16 in the hybrid, D. nobile × candidum. The methanol extract taken of them was also examined for cytotoxicity against FaDu human hypopharynx squamous carcinoma cells, where Dendrobii Herba showed the greatest cytotoxicity. In the untargeted metabolite analysis of 436 mutant lines of the hybrid, using UPLC-QToF MS and cytotoxicity measurements combined with multivariate analysis, two tentative flavonoids (M1 and M2) were evaluated as key markers among the analyzed metabolites, contributing to the distinction between active and inactive mutant lines.

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Keratinocyte Growth Factor Induces Gene Expression Signature Associated with Suppression of Malignant Phenotype of Cutaneous Squamous Carcinoma Cells

PLoS ONE ◽

10.1371/journal.pone.0033041 ◽

2012 ◽

Vol 7 (3) ◽

pp. e33041 ◽

Cited By ~ 14

Author(s):

Mervi Toriseva ◽

Risto Ala-aho ◽

Sirkku Peltonen ◽

Juha Peltonen ◽

Reidar Grénman ◽

...

Keyword(s):

Gene Expression ◽

Growth Factor ◽

Keratinocyte Growth Factor ◽

Gene Expression Signature ◽

Squamous Carcinoma ◽

Carcinoma Cells ◽

Malignant Phenotype ◽

Expression Signature ◽

Squamous Carcinoma Cells

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Antibody-induced epidermal growth factor receptor dimerization mediates inhibition of autocrine proliferation of A431 squamous carcinoma cells.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)47026-3 ◽

1994 ◽

Vol 269 (44) ◽

pp. 27595-27602

Author(s):

Z Fan ◽

Y Lu ◽

X Wu ◽

J Mendelsohn

Keyword(s):

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Growth Factor Receptor ◽

Squamous Carcinoma ◽

Carcinoma Cells ◽

Receptor Dimerization ◽

Squamous Carcinoma Cells ◽

Epidermal Growth

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Knockdown of long non-coding RNA RP11-297P16.3 inhibits the migration and invasion of laryngeal squamous carcinoma cells

Clinical & Translational Oncology ◽

10.1007/s12094-021-02609-6 ◽

2021 ◽

Author(s):

H. Wu ◽

W. Wang ◽

J. Zhu

Keyword(s):

Squamous Carcinoma ◽

Carcinoma Cells ◽

Migration And Invasion ◽

Squamous Carcinoma Cells ◽

Non Coding Rna ◽

Long Non Coding Rna

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Cytotoxic Influence of Khat (Catha edulis (Vahl) Forssk. ex Endl) on Oral Fibroblasts, Squamous Carcinoma Cells, and Expression of α Smooth Muscle Actin

Applied Sciences ◽

10.3390/app11083524 ◽

2021 ◽

Vol 11 (8) ◽

pp. 3524

Author(s):

Azeem Ul Yaqin Syed ◽

Muhammad A. Ahmed ◽

Eman I. AlSagob ◽

Mansour Al-Askar ◽

Abdulrahman M. AlMubarak ◽

...

Keyword(s):

Cell Death ◽

Smooth Muscle ◽

Smooth Muscle Actin ◽

Control Cell ◽

Squamous Carcinoma ◽

Carcinoma Cells ◽

Muscle Actin ◽

Catha Edulis ◽

Squamous Carcinoma Cells ◽

Dose Dependent

The aim was to determine the cytotoxicity of Khat (Catha edulis (Vahl) Forssk. ex Endl) on normal oral fibroblasts (NOFs) and SCC4 (squamous carcinoma cells) along with expression of α-smooth muscle actin (α-SMA) in fibroblasts. Khat filtrate was prepared to obtain a concentrated viscous solution. NOFs and SCC4 cells were cultured in biological cabinets and were grown in Dulbeccos’ modified Eagles medium. Frozen cells were thawed at 37 °C and cell seeding was performed. NOFs and SCC4 cells were seeded on 96 well plates and allowed to attach. The medium was removed and a fresh medium containing different concentrations of Khat was added. The group without Khat served as a negative control and 4% paraformaldehyde as the positive control. Cell viability was assessed using the MTT assay and effect of Khat on fibroblast and SCC4 phenotypes was evaluated by immunostaining. Analysis of variance was used to assess data (p < 0.05). NOF 316 showed cell death in response to 4% paraformaldehyde, 12.5, 6.25, and 3.12 mg/mL of Khat. The highest concentration of Khat (25 mg/mL) failed to cause cytotoxicity of NOF 316. NOF 319 and NOF 26 displayed cell death at all concentrations of Khat, however, cytotoxicity was not dose dependent. NOF 18 and SCC4 cells showed dose-dependent cell death. NOF 316 showed α-SMA expression after 1 mg/mL of Khat exposure. Not all fibroblasts were α-SMA-positive, suggesting specific activation of a subset of fibroblasts. Khat is cytotoxic to NOF and SCC4 cells. Furthermore, it can also cause activation and phenotypic changes in oral fibroblasts, indicating a potential role in progression of oral squamous cell carcinoma.

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