Alleviation of Lead Stress on Sage Plant by 5-Aminolevulinic Acid (ALA)

Oxidative stress is imparted by a varying range of environmental factors involving heavy metal stress. Thus, the mechanisms of antioxidant resistance may advance a policy to improve metal tolerance. Lead as a toxic heavy metal negatively affects the metabolic activities and growth of medicinal and aromatic plants. This investigation aimed to assess the function of 5-aminolevulinic acid (ALA) in the alleviation of Pb stress in sage plants (Salvia officinalis L.) grown either hydroponically or in pots. Various concentrations of Pb (0, 100, 200, and 400 µM) and different concentrations of ALA (0, 10, and 20 mg L−1) were tested. This investigation showed that Pb altered the physiological parameters. Pb stress differentially reduced germination percentage and protein content compared to control plants. However, lead stress promoted malondialdehyde (MDA) and H2O2 contents in the treated plants. Also, lead stress enhanced the anti-oxidative enzyme activities; ascorbate peroxidase superoxide, dismutase, glutathione peroxidase, and glutathione reductase in Salvia plants. ALA application enhanced the germination percentage and protein content compared to their corresponding controls. Whereas, under ALA application MDA and H2O2 contents, as well as the activities of SOD, APX, GPX, and GR, were lowered. These findings suggest that ALA at the 20 mgL−1 level protects the Salvia plant from Pb stress. Therefore, the results recommend ALA application to alleviate Pb stress.

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Metal-responsive transcription factor (MTF-1) and heavy metal stress response in Drosophila and mammalian cells: a functional comparison

Biological Chemistry ◽

10.1515/bc.2004.074 ◽

2004 ◽

Vol 385 (7) ◽

pp. 597-603 ◽

Cited By ~ 39

Author(s):

K. Balamurugan ◽

D. Egli ◽

A. Selvaraj ◽

B. Zhang ◽

O. Georgiev ◽

...

Keyword(s):

Heavy Metal ◽

Transcription Factor ◽

Mammalian Cells ◽

Metal Tolerance ◽

Mammalian Cell Culture ◽

Heavy Metal Stress ◽

Targeted Disruption ◽

Toxic Heavy Metal ◽

Liver Degeneration ◽

Transcription Factor 1

AbstractThe zinc finger transcription factor MTF-1 (metalresponsive transcription factor-1) is conserved from insects to vertebrates. Its major role in both organisms is to control the transcription of genes involved in the homeostasis and detoxification of heavy metal ions such as Cu[2+], Zn[2+] and Cd [2+]. In mammals, MTF-1 serves at least two additional roles. First, targeted disruption of the MTF-1 gene results in death at embryonic day 14 due to liver degeneration, revealing a stagespecific developmental role. Second, under hypoxicanoxic stress, MTF-1 helps to activate the transcription of the gene placental growth factor (PlGF), an angiogenic protein. Recently we characterized dMTF-1, theDrosophilahomolog of mammalian MTF-1. Here we present a series of studies to compare the metal response in mammals and insects, which reveal common features but also differences. A human MTF-1 transgene can restore to a large extent metal tolerance to flies lacking their own MTF-1 gene, both at low and high copper concentrations. Likewise,DrosophilaMTF-1 can substitute for human MTF-1 in mammalian cell culture, although both the basal and the metalinduced transcript levels are lower. Finally, a clear difference was revealed in the response to mercury, a highly toxic heavy metal: metallothioneintype promoters respond poorly, if at all, to Hg[2+] in mammalian cells but strongly inDrosophila, and this response is completely dependent on dMTF-1.

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Effect of Lead and Copper on Photosynthetic Apparatus in Citrus (Citrus aurantium L.) Plants. The Role of Antioxidants in Oxidative Damage as a Response to Heavy Metal Stress

Plants ◽

10.3390/plants10010155 ◽

2021 ◽

Vol 10 (1) ◽

pp. 155

Author(s):

Anastasia Giannakoula ◽

Ioannis Therios ◽

Christos Chatzissavvidis

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Heavy Metal ◽

Metal Tolerance ◽

Heavy Metal Tolerance ◽

Membrane Integrity ◽

Photosynthetic Apparatus ◽

Heavy Metal Stress ◽

Photosynthetic Parameters ◽

Citrus Aurantium

Photosynthetic changes and antioxidant activity to oxidative stress were evaluated in sour orange (Citrus aurantium L.) leaves subjected to lead (Pb), copper (Cu) and also Pb + Cu toxicity treatments, in order to elucidate the mechanisms involved in heavy metal tolerance. The simultaneous effect of Pb− and Cu on growth, concentration of malondialdehyde (MDA), hydrogen peroxide (H2O2), chlorophylls, flavonoids, carotenoids, phenolics, chlorophyll fluorescence and photosynthetic parameters were examined in leaves of Citrus aurantium L. plants. Exogenous application of Pb and Cu resulted in an increase in leaf H2O2 and lipid peroxidation (MDA). Toxicity symptoms of both Pb and Cu treated plants were stunted growth and decreased pigments concentration. Furthermore, photosynthetic activity of treated plants exhibited a significant decline. The inhibition of growth in Pb and Cu-treated plants was accompanied by oxidative stress, as indicated by the enhanced lipid peroxidation and the high H2O2 concentration. Furthermore, antioxidants in citrus plants after exposure to high Pb and Cu concentrations were significantly increased compared to control and low Pb and Cu treatments. In conclusion, this study indicates that Pb and Cu promote lipid peroxidation, disrupt membrane integrity, reduces growth and photosynthesis and inhibit mineral nutrition. Considering the potential for adverse human health effects associated with high concentrations of Pb and Cu contained in edible parts of citrus plants the study signals that it is important to conduct further research into the accessibility and uptake of the tested heavy metals in the soil and whether they pose risks to humans.

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Over-expression of chickpea metallothionein 1 gene confers tolerance against major toxic heavy metal stress in Arabidopsis

Physiology and Molecular Biology of Plants ◽

10.1007/s12298-021-01103-1 ◽

2021 ◽

Author(s):

Arvind Kumar Dubey ◽

Anil Kumar ◽

Navin Kumar ◽

Sanoj Kumar ◽

Meenakshi ◽

...

Keyword(s):

Heavy Metal ◽

Heavy Metal Stress ◽

Metal Stress ◽

Toxic Heavy Metal ◽

Over Expression

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5-Aminolevulinic Acid-Induced Heavy Metal Stress Tolerance and Underlying Mechanisms in Plants

Journal of Plant Growth Regulation ◽

10.1007/s00344-018-9875-y ◽

2018 ◽

Vol 37 (4) ◽

pp. 1423-1436 ◽

Cited By ~ 12

Author(s):

Shafaqat Ali ◽

Muhammad Rizwan ◽

Abbu Zaid ◽

Muhammad Saleem Arif ◽

Tahira Yasmeen ◽

...

Keyword(s):

Heavy Metal ◽

Stress Tolerance ◽

Aminolevulinic Acid ◽

Heavy Metal Stress ◽

Metal Stress ◽

Underlying Mechanisms

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Heavy metal tolerance of Pontechium maculatum (Boraginaceae) from several ultramafic localities in Serbia

Botanica Serbica ◽

10.2298/botserb1901073j ◽

2019 ◽

Vol 43 (1) ◽

pp. 73-83 ◽

Cited By ~ 3

Author(s):

Ksenija Jakovljevic ◽

Sanja Djurovic ◽

Mina Antusevic ◽

Nevena Mihailovic ◽

Uros Buzurovic ◽

...

Keyword(s):

Heavy Metal ◽

Metal Tolerance ◽

Heavy Metal Tolerance ◽

Heavy Metal Stress ◽

Balkan Peninsula ◽

Major And Trace Elements ◽

Soil Samples ◽

Plant Tissues ◽

Trace Element Profiles ◽

Range Of Values

Pontechium maculatum, a facultative metallophyte, was collected from four ultramafic localities in Serbia and analysed in terms of micro- and macroelement accumulation. The aim of the study was to reveal trace element profiles and differences in uptake and translocation of heavy metals in populations growing under heavy metal stress. The concentrations of major and trace elements in soil samples (Ca, Mg, Fe, Mn, Ni, Pb, Cr, Zn, Cu, Co, Cd) and in plant tissues (Fe, Mn, Ni, Pb, Cr, Zn, Cu, Co, Cd) are presented. The results of our analysis indicate that P. maculatum efficiently absorbs Zn and Cr, while for most of the other elements accumulation levels fit in the range of values obtained for several other species from ultramafic localities on the Balkan Peninsula.

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Overcoming the Bacteriostatic Effects of Heavy Metals on A. thiooxidans Direct Bioleaching of Saprolitic Ni Laterite Ores

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1130.263 ◽

2015 ◽

Vol 1130 ◽

pp. 263-267 ◽

Cited By ~ 1

Author(s):

Hee Chan Jang ◽

Marjorie Valix

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Metal Tolerance ◽

Heavy Metal Tolerance ◽

Heavy Metal Stress ◽

Lag Phase ◽

Tolerance Index ◽

Acid Generation ◽

Laterite Ores ◽

Laterite Ore

In this study, the adaptation of A. thiooxidans to heavy metals leached from saprolitic Ni laterite ores was performed by gradual acclimatisation. The bacteria was cultivated in heavy metals (Ni, Co, Fe, Mg, Cr and Mn) with total concentrations of 2400 to 24000 ppm equivalent to total dissolution of 1 to 10% (w/v) pulp densities of the saprolitic Ni laterite ore. Adaptation evolution mapped from its tolerance index was found to be dependent on metal concentration, acid generation, and period of adaptation. Bio-stimulation of cell growth and acid production was promoted by heavy metal stress on the bacteria. Pre-established heavy metal tolerance of the bacteria improved the leaching rate in its early phase; 20% and 7% increase in Ni and Co metal recoveries were observed in using adapted bacteria. However heavy metal tolerance was also achieved by the bacteria during the leaching process, albeit delayed by a lag phase. These results confirm the robust nature and suitability of A. thiooxidans in direct biomining of Ni ores.

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Genome-Wide Identification and Expression Analysis of Metal Tolerance Protein Gene Family in Medicago truncatula Under a Broad Range of Heavy Metal Stress

Frontiers in Genetics ◽

10.3389/fgene.2021.713224 ◽

2021 ◽

Vol 12 ◽

Author(s):

Ahmed H. El-Sappah ◽

Rania G. Elbaiomy ◽

Ahmed S. Elrys ◽

Yu Wang ◽

Yumin Zhu ◽

...

Keyword(s):

Heavy Metal ◽

Gene Ontology ◽

Gene Family ◽

Medicago Truncatula ◽

Metal Ion ◽

Metal Tolerance ◽

Functional Characterization ◽

Heavy Metal Stress ◽

Metal Stress ◽

Gene Similarity

Metal tolerance proteins (MTPs) encompass plant membrane divalent cation transporters to specifically participate in heavy metal stress resistance and mineral acquisition. However, the molecular behaviors and biological functions of this family in Medicago truncatula are scarcely known. A total of 12 potential MTP candidate genes in the M. truncatula genome were successfully identified and analyzed for a phylogenetic relationship, chromosomal distributions, gene structures, docking analysis, gene ontology, and previous gene expression. M. truncatula MTPs (MtMTPs) were further classified into three major cation diffusion facilitator (CDFs) groups: Mn-CDFs, Zn-CDFs, and Fe/Zn-CDFs. The structural analysis of MtMTPs displayed high gene similarity within the same group where all of them have cation_efflux domain or ZT_dimer. Cis-acting element analysis suggested that various abiotic stresses and phytohormones could induce the most MtMTP gene transcripts. Among all MTPs, PF16916 is the specific domain, whereas GLY, ILE, LEU, MET, ALA, SER, THR, VAL, ASN, and PHE amino acids were predicted to be the binding residues in the ligand-binding site of all these proteins. RNA-seq and gene ontology analysis revealed the significant role of MTP genes in the growth and development of M. truncatula. MtMTP genes displayed differential responses in plant leaves, stems, and roots under five divalent heavy metals (Cd2+, Co2+, Mn2+, Zn2+, and Fe2+). Ten, seven, and nine MtMTPs responded to at least one metal ion treatment in the leaves, stems, and roots, respectively. Additionally, MtMTP1.1, MtMTP1.2, and MtMTP4 exhibited the highest expression responses in most heavy metal treatments. Our results presented a standpoint on the evolution of MTPs in M. truncatula. Overall, our study provides a novel insight into the evolution of the MTP gene family in M. truncatula and paves the way for additional functional characterization of this gene family.

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Populus deltoides Kunitz trypsin inhibitor 3 confers metal tolerance and binds copper, revealing a new defensive role against heavy metal stress

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2015.02.005 ◽

2015 ◽

Vol 115 ◽

pp. 28-37 ◽

Cited By ~ 8

Author(s):

Fernando P. Guerra ◽

Luz Reyes ◽

Ariela Vergara-Jaque ◽

Carola Campos-Hernández ◽

Adelina Gutiérrez ◽

...

Keyword(s):

Heavy Metal ◽

Trypsin Inhibitor ◽

Populus Deltoides ◽

Metal Tolerance ◽

Heavy Metal Stress ◽

Metal Stress ◽

Kunitz Trypsin Inhibitor ◽

Defensive Role

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Role of Silicon in Mitigation of Heavy Metal Stresses in Crop Plants

Plants ◽

10.3390/plants8030071 ◽

2019 ◽

Vol 8 (3) ◽

pp. 71 ◽

Cited By ~ 39

Author(s):

Javaid Akhter Bhat ◽

S. M. Shivaraj ◽

Pritam Singh ◽

Devanna B. Navadagi ◽

Durgesh Kumar Tripathi ◽

...

Keyword(s):

Heavy Metal ◽

Metal Ions ◽

Metal Tolerance ◽

Abiotic Factors ◽

Heavy Metal Tolerance ◽

Heavy Metal Stress ◽

Crop Plants ◽

Metal Stress ◽

Growth And Yield

Over the past few decades, heavy metal contamination in soil and water has increased due to anthropogenic activities. The higher exposure of crop plants to heavy metal stress reduces growth and yield, and affect the sustainability of agricultural production. In this regard, the use of silicon (Si) supplementation offers a promising prospect since numerous studies have reported the beneficial role of Si in mitigating stresses imposed by biotic as well as abiotic factors including heavy metal stress. The fundamental mechanisms involved in the Si-mediated heavy metal stress tolerance include reduction of metal ions in soil substrate, co-precipitation of toxic metals, metal-transport related gene regulation, chelation, stimulation of antioxidants, compartmentation of metal ions, and structural alterations in plants. Exogenous application of Si has been well documented to increase heavy metal tolerance in numerous plant species. The beneficial effects of Si are particularly evident in plants able to accumulate high levels of Si. Consequently, to enhance metal tolerance in plants, the inherent genetic potential for Si uptake should be improved. In the present review, we have discussed the potential role and mechanisms involved in the Si-mediated alleviation of metal toxicity as well as different approaches for enhancing Si-derived benefits in crop plants.

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Melatonin Confers Heavy Metal Tolerance of a Dark Septate Endophyte (DSE) Exophiala Pisciphila Via Lowering Oxidative Stress and Heavy Metal Accumulation

10.21203/rs.3.rs-54820/v1 ◽

2020 ◽

Author(s):

Yang Yu ◽

Zhaowei Teng ◽

Zongmin Mou ◽

Yan Lv ◽

Tao Li ◽

...

Keyword(s):

Oxidative Stress ◽

Heavy Metal ◽

Metal Accumulation ◽

Metal Tolerance ◽

Heavy Metal Tolerance ◽

Heavy Metal Stress ◽

Heavy Metal Accumulation ◽

Metal Stress ◽

Dark Septate Endophyte ◽

Exophiala Pisciphila

Abstract Background: The high antioxidant capacity of melatonin contributing to heavy metal tolerance for plants and animals is widely studied, while researches on microorganisms especially in filamentous fungi are rare. One typical dark septate endophyte (DSE), Exophiala pisciphila, showed significant resistance to heavy metals.Results: In this study, exogenous melatonin was verified to reduce heavy metal damage via relieving oxidative stress, activating antioxidant systems, and decreasing heavy metal accumulation in E. pisciphila. Melatonin biosynthesis enzyme genes were upregulated under heavy metal stress. Furthermore, the overexpression of E. pisciphila TDC1 (EpTDC1) and E. pisciphila ASMT1 (EpASMT1) responsible for melatonin biosynthesis in Escherichia coli and Arabidopsis thaliana, enhanced heavy metal stress tolerance for the two organisms by lowering the oxidative stress and reducing the Cd accumulation in the whole plants, especially in the roots.Conclusions: Our results indicate that melatonin confers heavy metal resistance in E. pisciphila by lowering oxidative stress and heavy metal accumulation.

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