scholarly journals Exploring the Phytochemicals of Acacia melanoxylon R. Br.

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2698
Author(s):  
Diana Alves ◽  
Sidónio Duarte ◽  
Pedro Arsénio ◽  
Joana Gonçalves ◽  
Cecília M. P. Rodrigues ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mass Spectrometry ◽  
Methyl Ester ◽  
Nmr Assignments ◽  
2D Nmr ◽  
Human Colorectal Cancer ◽  
2D Nmr Spectroscopy ◽  
Acacia Melanoxylon ◽  
Colorectal Cancer Cells ◽  
Human Colorectal Cancer Cells

Invasive species are currently a world menace to the environment, although the study of their chemistry may provide a means for their future beneficial use. From a study of Portuguese Acacia melanoxylon R. Br. five known compounds were isolated: lupeol, 3β-Z-coumaroyl lupeol, 3β-E-coumaroyl lupeol (dioslupecin A), kolavic acid 15-methyl ester and vomifoliol (blumenol A). Their structures were elucidated by 1D and 2D NMR spectroscopy and mass spectrometry, and as a result some corrections are made to their previous 13C NMR assignments. Cytotoxicity of 3β-E-coumaroyl lupeol (dioslupecin A) and kolavic acid 15-methyl ester was evaluated against HCT116 human colorectal cancer cells although biological activity was not evident.

scholarly journals Coptidis Rhizoma Extract Reverses 5-Fluorouracil Resistance in hct116 Human Colorectal Cancer Cells via Modulation of Thymidylate Synthase

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1856
Author(s):  
Yong-Hwi Kang ◽  
Jin-Seok Lee ◽  
Nam-Hun Lee ◽  
Seung-Hyung Kim ◽  
Chang-Seob Seo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Thymidylate Synthase ◽  
Cell Cycle Distribution ◽  
Human Colorectal Cancer ◽  
Common Cancer ◽  
G1 Phase Arrest ◽  
Colorectal Cancer Cells ◽  
Coptidis Rhizoma ◽  
Underlying Mechanisms ◽  
Human Colorectal Cancer Cells

Colorectal cancer (CRC) is a malignancy of the colon or rectum. It is ranked as the third most common cancer in both men and women worldwide. Early resection permitted by early detection is the best treatment, and chemotherapy is another main treatment, particularly for patients with advanced CRC. A well-known thymidylate synthase (TS) inhibitor, 5-fluorouracil (5-FU), is frequently prescribed to CRC patients; however, drug resistance is a critical limitation of its clinical application. Based on the hypothesis that Coptidis Rhizoma extract (CRE) can abolish this 5-FU resistance, we explored the efficacy and underlying mechanisms of CRE in 5-FU-resistant (HCT116/R) and parental HCT116 (HCT116/WT) cells. Compared to treatment with 5-FU alone, combination treatment with CRE and 5-FU drastically reduced the viability of HCT116/R cells. The cell cycle distribution assay showed significant induction of the G0/G1 phase arrest by co-treatment with CRE and 5-FU. In addition, the combination of CRE and 5-FU notably suppressed the activity of TS, which was overexpressed in HCT116/R cells, as compared to HCT116/WT cells. Our findings support the potential of CRE as an adjuvant agent against 5-FU-resistant colorectal cancers and indicate that the underlying mechanisms might involve inhibition of TS expression.

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