scholarly journals Rapid, Single-Step Protein Encapsulation via Flash NanoPrecipitation

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1406 ◽  
Author(s):  
Shani L. Levit ◽  
Rebecca C. Walker ◽  
Christina Tang
Keyword(s):  
Self Assembly ◽  
Electrostatic Interactions ◽  
Cationic Polyelectrolyte ◽  
Single Step ◽  
Protein Encapsulation ◽  
Flash Nanoprecipitation ◽  
Hydrophobic Materials ◽  
Model Protein ◽  
Processing Steps ◽  
Multiple Processing

Flash NanoPrecipitation (FNP) is a rapid method for encapsulating hydrophobic materials in polymer nanoparticles with high loading capacity. Encapsulating biologics such as proteins remains a challenge due to their low hydrophobicity (logP < 6) and current methods require multiple processing steps. In this work, we report rapid, single-step protein encapsulation via FNP using bovine serum albumin (BSA) as a model protein. Nanoparticle formation involves complexation and precipitation of protein with tannic acid and stabilization with a cationic polyelectrolyte. Nanoparticle self-assembly is driven by hydrogen bonding and electrostatic interactions. Using this approach, high encapsulation efficiency (up to ~80%) of protein can be achieved. The resulting nanoparticles are stable at physiological pH and ionic strength. Overall, FNP is a rapid, efficient platform for encapsulating proteins for various applications.

scholarly journals Single-Step Self-Assembly and Physical Crosslinking of PEGylated Chitosan Nanoparticles by Tannic Acid

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 749 ◽  
Author(s):  
Raven A. Smith ◽  
Rebecca C. Walker ◽  
Shani L. Levit ◽  
Christina Tang
Keyword(s):  
Tannic Acid ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Hydrophobic Interactions ◽  
Chitosan Nanoparticles ◽  
Single Step ◽  
Step Method ◽  
Core Mass ◽  
Solids Concentration ◽  
Formulation Parameters

Chitosan-based nanoparticles are promising materials for potential biomedical applications. We used Flash NanoPrecipitation as a rapid, scalable, single-step method to achieve self-assembly of crosslinked chitosan nanoparticles. Self-assembly was driven by electrostatic interactions, hydrogen bonding, and hydrophobic interactions; tannic acid served to precipitate chitosan to seed nanoparticle formation and crosslink the chitosan to stabilize the resulting particles. The size of the nanoparticles can be tuned by varying formulation parameters including the total solids concentration and block copolymer to core mass ratio. We demonstrated that hydrophobic moieties can be incorporated into the nanoparticle using a lipophilic fluorescent dye as a model system.

In situ Time-Resolved Small-Angle X-ray Scattering Reveals the Formation Kinetics of Polyelectrolyte Complex Micelles

2019 ◽  
Author(s):  
Hao Wu ◽  
Jeffrey Ting ◽  
Siqi Meng ◽  
Matthew Tirrell
Keyword(s):  
Small Angle ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Polyelectrolyte Complex ◽  
Time Resolved ◽  
X Ray ◽  
X Ray Scattering ◽  
Kinetics Of ◽  
Ray Scattering

We have directly observed the <i>in situ</i> self-assembly kinetics of polyelectrolyte complex (PEC) micelles by synchrotron time-resolved small-angle X-ray scattering, equipped with a stopped-flow device that provides millisecond temporal resolution. This work has elucidated one general kinetic pathway for the process of PEC micelle formation, which provides useful physical insights for increasing our fundamental understanding of complexation and self-assembly dynamics driven by electrostatic interactions that occur on ultrafast timescales.

scholarly journals FAIRSCAPE: a Framework for FAIR and Reproducible Biomedical Analytics

2021 ◽  
Author(s):  
Maxwell Adam Levinson ◽  
Justin Niestroy ◽  
Sadnan Al Manir ◽  
Karen Fairchild ◽  
Douglas E. Lake ◽  
...  
Keyword(s):  
Computational Result ◽  
Large Scale ◽  
Graph Model ◽  
Inferential Reasoning ◽  
Computational Framework ◽  
Textual Description ◽  
Evidence Graph ◽  
Computational Analyses ◽  
Processing Steps ◽  
Multiple Processing

AbstractResults of computational analyses require transparent disclosure of their supporting resources, while the analyses themselves often can be very large scale and involve multiple processing steps separated in time. Evidence for the correctness of any analysis should include not only a textual description, but also a formal record of the computations which produced the result, including accessible data and software with runtime parameters, environment, and personnel involved. This article describes FAIRSCAPE, a reusable computational framework, enabling simplified access to modern scalable cloud-based components. FAIRSCAPE fully implements the FAIR data principles and extends them to provide fully FAIR Evidence, including machine-interpretable provenance of datasets, software and computations, as metadata for all computed results. The FAIRSCAPE microservices framework creates a complete Evidence Graph for every computational result, including persistent identifiers with metadata, resolvable to the software, computations, and datasets used in the computation; and stores a URI to the root of the graph in the result’s metadata. An ontology for Evidence Graphs, EVI (https://w3id.org/EVI), supports inferential reasoning over the evidence. FAIRSCAPE can run nested or disjoint workflows and preserves provenance across them. It can run Apache Spark jobs, scripts, workflows, or user-supplied containers. All objects are assigned persistent IDs, including software. All results are annotated with FAIR metadata using the evidence graph model for access, validation, reproducibility, and re-use of archived data and software.

Biocompatible, chimeric peptide-condensed supramolecular nanoparticles for tumor cell-specific siRNA delivery and gene silencing

2014 ◽  
Vol 50 (58) ◽  
pp. 7806-7809 ◽  
Author(s):  
Hangxiang Wang ◽  
Wei Chen ◽  
Haiyang Xie ◽  
Xuyong Wei ◽  
Shengyong Yin ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Gene Silencing ◽  
Delivery System ◽  
Self Assembly ◽  
Sirna Delivery ◽  
Single Step ◽  
Chimeric Peptide ◽  
Supramolecular Nanoparticles ◽  
Sirna Delivery System

A practical and tumor cell-specific siRNA delivery system was developedviasingle-step self-assembly of an arginine-rich chimeric peptide with siRNA.

scholarly journals Direct, single-step synthesis of hierarchical zeolites without secondary templating

2015 ◽  
Vol 3 (3) ◽  
pp. 1298-1305 ◽  
Author(s):  
Zhuopeng Wang ◽  
Chao Li ◽  
Hong Je Cho ◽  
Shih-Chieh Kung ◽  
Mark A. Snyder ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Mass Transport ◽  
Self Assembly ◽  
Single Step ◽  
Hierarchical Zeolites ◽  
Hierarchical Zeolite ◽  
Initial Stage ◽  
Zeolite Precursors

Hierarchical ZSM-5 with a shell of stacked coffin-shaped crystals and a core of nanocrystal aggregates was synthesized by controlling the formation and self-assembly of zeolite precursors formed in the initial stage of crystallization. The formed hierarchical zeolite shows superior catalytic activity for reaction involving bulky molecules due to enhanced mass transport.

Single-step self-assembly of multilayer graphene based dielectric nanostructures

FlatChem ◽  
2017 ◽  
Vol 4 ◽  
pp. 61-67 ◽  
Author(s):  
Jeong Eun Baek ◽  
Ju Young Kim ◽  
Hyeong Min Jin ◽  
Bong Hoon Kim ◽  
Kyung Eun Lee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Single Step ◽  
Multilayer Graphene

Research on the self-assembly of exfoliated perovskite nanosheets (LaNb2O7−) and cobalt porphyrin utilized for the electrocatalytic oxidation of ascorbic acid

RSC Advances ◽  
2016 ◽  
Vol 6 (52) ◽  
pp. 46388-46393 ◽  
Author(s):  
Binbin Pan ◽  
Wenlei Zhao ◽  
Xiaobo Zhang ◽  
Jinpeng Li ◽  
Jiasheng Xu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Electrocatalytic Oxidation ◽  
Hybrid Film ◽  
The Self ◽  
Cobalt Porphyrin

A sandwich-structured nanocomposite of LaNb2O7/CoTMPyP was fabricated via electrostatic interactions between LaNb2O7− nanosheets and cobalt porphyrin cations, and the obtained hybrid film exhibited excellent electrocatalytic activities toward AA.

scholarly journals IRONSperm: Sperm-templated soft magnetic microrobots

2020 ◽  
Vol 6 (28) ◽  
pp. eaba5855 ◽  
Author(s):  
Veronika Magdanz ◽  
Islam S. M. Khalil ◽  
Juliane Simmchen ◽  
Guilherme P. Furtado ◽  
Sumit Mohanty ◽  
...  
Keyword(s):  
Self Assembly ◽  
Drug Loading ◽  
Chemotherapeutic Agents ◽  
Single Step ◽  
Biological Entity ◽  
Sperm Cells ◽  
Soft Magnetic ◽  
Actuation Frequency ◽  
Magnetic Microrobots

We develop biohybrid magnetic microrobots by electrostatic self-assembly of nonmotile sperm cells and magnetic nanoparticles. Incorporating a biological entity into microrobots entails many functional advantages beyond shape templating, such as the facile uptake of chemotherapeutic agents to achieve targeted drug delivery. We present a single-step electrostatic self-assembly technique to fabricate IRONSperms, soft magnetic microswimmers that emulate the motion of motile sperm cells. Our experiments and theoretical predictions show that the swimming speed of IRONSperms exceeds 0.2 body length/s (6.8 ± 4.1 µm/s) at an actuation frequency of 8 Hz and precision angle of 45°. We demonstrate that the nanoparticle coating increases the acoustic impedance of the sperm cells and enables localization of clusters of IRONSperm using ultrasound feedback. We also confirm the biocompatibility and drug loading ability of these microrobots, and their promise as biocompatible, controllable, and detectable biohybrid tools for in vivo targeted therapy.

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