Release of Bisphenol A from Pit and Fissure Sealants According to Different pH Conditions

Changes in intraoral pH can cause changes in the chemical decomposition and surface properties of treated resin-based pits and fissure sealants (sealant). The purpose of this study is to evaluate the release of bisphenol A (BPA) from sealants under three different pH conditions over time. The test specimen was applied with 6 sealants 5 mg each on a glass plate (10 × 10 mm) and photopolymerized. The samples were immersed for 10 min, 1 h, and 24 h in solutions of pH 3.0, 6.5, and 10.0 at 37 °C. BPA release was measured using a gas chromatography-mass spectrometer. A statistical analysis was performed by two-way ANOVA and one-way ANOVA to verify the effect of pH conditions and time on BPA release. The BPA concentration in the pH 3.0 group was higher at all points than with pH 6.5 and pH 10.0 (p < 0.05), and gradually increased over time (p < 0.05). As a result, it was confirmed that low pH negatively influences BPA release. Therefore, frequent exposure to low pH due to the consumption of various beverages after sealant treatment can negatively affect the sealant’s chemical stability in the oral cavity.

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In vivo bisphenol-A release from dental pit and fissure sealants: A systematic review

Journal of Dentistry ◽

10.1016/j.jdent.2013.04.012 ◽

2013 ◽

Vol 41 (8) ◽

pp. 659-667 ◽

Cited By ~ 41

Author(s):

Dimitrios Kloukos ◽

Nikolaos Pandis ◽

Theodore Eliades

Keyword(s):

Systematic Review ◽

Bisphenol A ◽

Fissure Sealants ◽

Pit And Fissure Sealants

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Comparing Bond Strength,Microleakage and Clinical Performance of Three Pit and Fissure Sealants

Case Medical Research ◽

10.31525/ct1-nct04272866 ◽

2020 ◽

Author(s):

Keyword(s):

Bond Strength ◽

Clinical Performance ◽

Fissure Sealants ◽

Pit And Fissure Sealants

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Knowledge Analysis of Pit and Fissure Sealants Among the Dental Students

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.sp2.031 ◽

2020 ◽

Vol 12 (sp2) ◽

Keyword(s):

Dental Students ◽

Fissure Sealants ◽

Pit And Fissure Sealants ◽

Knowledge Analysis

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Potential of arsenic bioremediation by a cyanobacterium isolated from the Salado River in the Atacama Desert

Journal of Plankton Research ◽

10.1093/plankt/fbab016 ◽

2021 ◽

Vol 43 (2) ◽

pp. 156-160

Author(s):

Pablo Pérez-Portilla ◽

Juan Araya ◽

Karem Gallardo ◽

Adriana Aránguiz-Acuña

Keyword(s):

Atacama Desert ◽

Aquatic Systems ◽

Arid Environments ◽

Northern Chile ◽

Evolutionary Mechanisms ◽

Alkaline Conditions ◽

Ph Conditions ◽

Removal Of Arsenic ◽

Salado River ◽

Over Time

Abstract Cyanobacteria and microalgae are recognized as excellent metal(loid)s-bioremediators of aquatic systems. We isolated a cyanobacterium from the Salado River in the Atacama Desert, northern Chile, which was identified as Cyanobium sp. Growth inhibition bioassays were conducted with arsenic and cadmium, and tolerance of Cyanobium to these metals was estimated. Removal of arsenic was assessed under different pH conditions and over time. We showed that the Cyanobium strain isolated from the Salado River has a greater tolerance to the arsenic and cadmium compounds than other species commonly used in metal(loid)s-bioremediation. Removal of up to 90% of arsenic was obtained in alkaline conditions, within the first 3 hours of exposure suggesting that Cyanobium sp. isolated from the Atacama Desert could be further studied with biotechnological purposes and to understand the evolutionary mechanisms of adaption to arid environments.

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Expression patterns of two carbonic anhydrase genes, Na+/K+-ATPase and V-type H+-ATPase, in the freshwater crayfish, Cherax quadricarinatus, exposed to low pH and high pH

Australian Journal of Zoology ◽

10.1071/zo16048 ◽

2017 ◽

Vol 65 (1) ◽

pp. 50 ◽

Cited By ~ 3

Author(s):

Muhammad Yousuf Ali ◽

Ana Pavasovic ◽

Peter B. Mather ◽

Peter J. Prentis

Keyword(s):

Carbonic Anhydrase ◽

Expression Patterns ◽

Maximum Level ◽

Low Ph ◽

Freshwater Crayfish ◽

Α Subunit ◽

Cherax Quadricarinatus ◽

High Ph ◽

Internal Ph ◽

Ph Conditions

Carbonic anhydrase (CA), Na+/K+-ATPase (NKA) and Vacuolar-type H+-ATPase (HAT) play vital roles in osmoregulation and pH balance in decapod crustaceans. As variable pH levels have a significant impact on the physiology of crustaceans, it is crucial to understand the mechanisms by which an animal maintains its internal pH. We examined expression patterns of cytoplasmic (CAc) and membrane-associated form (CAg) of CA, NKA α subunit and HAT subunit a in gills of freshwater crayfish, Cherax quadricarinatus, at three pH levels – 6.2, 7.2 (control) and 8.2 – over 24 h. Expression levels of CAc were significantly increased at low pH and decreased at high pH conditions 24 h after transfer. Expression increased at low pH after 12 h, and reached its maximum level by 24 h. CAg showed a significant increase in expression at 6 h after transfer at low pH. Expression of NKA significantly increased at 6 h after transfer to pH 6.2 and remained elevated for up to 24 h. Expression for HAT and NKA showed similar patterns, where expression significantly increased 6 h after transfer to low pH and remained significantly elevated throughout the experiment. Overall, CAc, CAg, NKA and HAT gene expression is induced at low pH conditions in freshwater crayfish.

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