scholarly journals Evaluation of the Validity of Digital Optical Microscopy in the Assessment of Marginal Adaptation of Dental Adhesive Interfaces

Polymers ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 83
Author(s):  
René Daher ◽  
Ivo Krejci ◽  
Enrico di Bella ◽  
Laurine Marger

Analysis of marginal adaptation of dental adhesive interfaces using scanning electron microscopy has proven to be a powerful nondestructive method to evaluate the quality of adhesion. This methodology is, however, time-consuming and needs expensive equipment. The purpose of this study was to evaluate the possibility and efficiency of using a digital optical microscope (DOM) to perform marginal analysis and to compare it with the scanning electron microscope (SEM) analysis. Fifteen defect-free molars were selected for this study. Class V cavities were prepared and restored with resin composite, and epoxy replicas were obtained from silicone impressions of the restored teeth. Custom-made image analysis software was then used to measure the percentage of the noncontinuous margins (NCM) of each sample. To compare the DOM to the gold standard, SEM, each sample was analyzed 10 times using the DOM and three times using the SEM, by the same experienced operator. The repeatability coefficient and concordance were evaluated, and a Bland and Altman analysis was used for comparison of the two methods of measurements. To validate the DOM analysis method, an ANOVA approach (Gage R R) was used. Repeatability and reproducibility, which are two components of precision to validate the DOM analysis system, were calculated. For this, the same restorations were analyzed by two additional operators three times with the DOM. The duration of each step of the analysis using both methods was also recorded as a secondary outcome. Regarding the repeatability of each method, the Friedman test showed no statistically significant difference within the repetitions of measurements by SEM and DOM (p = 0.523 and p = 0.123, respectively). Moreover, the Bland-Altman analysis revealed a bias of 0.86 and concluded no statistically significant difference between the two methods, DOM and SEM. ANOVA evaluated DOM measurement system variation including the variances of repeatability and reproducibility that represented, respectively, 0.3% and 4% of the variance components. Reproducibility or inter-operator variability represented the principal source of variability with a statistically significant difference (p = 0.024). The time required for analysis with SEM was almost double that of DOM. The use of digital optical microscopy appears to be a valid alternative to the SEM for the analysis of marginal adaptation of dental adhesive interfaces. Further studies to evaluate the effect of training of operators in digital optical microscopy could reveal higher accuracy for this method and inter-operator agreement when experience is gained.

2020 ◽  
Vol 45 (5) ◽  
pp. 473-483 ◽  
Author(s):  
CAGA Costa ◽  
NLG Albuquerque ◽  
JS Mendonça ◽  
AD Loguercio ◽  
VPA Saboia ◽  
...  

Clinical Relevance At 24 months, the dentin pretreatment with epigallocatechin-3-gallate did not impair the clinical performance of the adhesive Single Bond Universal regardless of the bonding strategy used. SUMMARY Purpose: To evaluate the two-year effect of dentin pretreatment with epigallocatechin-3-gallate (EGCG) on the clinical performance of restorations of noncarious cervical lesions (NCCLs) with Single Bond Universal, applied in two different modes (self-etch and etch-and-rinse). Methods and Materials: In this randomized clinical trial, 33 volunteers were selected, and 156 NCCLs were assigned to four groups: ER, etch-and-rinse; ER-EGCG, 0.1% EGCG dentin pretreatment + etch-and-rinse; SE, self-etch; and SE-EGCG, 0.1% EGCG dentin pretreatment + self-etch. The NCCLs were restored with a nanofilled resin composite and evaluated at baseline and at six, 12, 18, and 24 months using FDI criteria for retention, marginal staining, marginal adaptation, caries, and postoperative sensitivity. Two evaluators were blinded to the treatments performed, and impressions were taken for resin replicas to allow indirect observations. Statistical analyses were performed with Kruskal-Wallis and McNemar tests with a significance level of 5%. Results: Six restorations (one from ER, two from SE, one from ER-EGCG, and two from SEEGCG) were lost at 24 months with no significant differences (p>0.05). The retention rates were 97.0% (ER and ER-EGCG), 94.1% (SE), and 94.2% (SE-EGCG). For marginal adaptation, a significant difference was detected between the baseline and 24 months for the SE group (p=0.0313). There were no statistical differences among all other evaluated criteria at 24 months, neither for each group at baseline nor for 24-month comparisons (p>0.05). Conclusions: The pretreatment with EGCG provided no benefit in the clinical performance of the adhesive regardless of the bonding strategy used. In addition, it adds an additional required step to the restorative procedure.


2013 ◽  
Vol 38 (5) ◽  
pp. 477-487 ◽  
Author(s):  
JO Burgess ◽  
R Sadid-Zadeh ◽  
D Cakir ◽  
LC Ramp

SUMMARY Objective: The purpose of this study was to compare the clinical performance of two self-etch dental adhesives with Single Bond Plus, a traditional one-bottle total-etch dental adhesive, for the restoration of noncarious cervical lesions. Materials and Methods: A total of 156 restorations were placed in noncarious cervical lesions with a minimum depth of 1.5 mm. Patients had no chronic periodontal disease and had normal salivary function. Each patient received restorations on three teeth, each bonded with either Adper Single Bond Plus, Adper Easy Bond, or Adper Scotchbond SE dental adhesive. All lesions were restored with Filtek Supreme Plus composite resin. All teeth were isolated with a rubber dam, received a short enamel bevel, and were cleaned with flour of pumice. The adhesives and resin composite were applied following the manufacturers' instructions. Restorations were clinically evaluated at baseline, six months, one year, and two years using modified US Public Health Service criteria. Results: Two-year retention was recorded as 97.3%, 90.5%, and 95.2%, for Single Bond Plus, Scotchbond SE, and Easy Bond, respectively. Statistical analysis did not show a significant difference (p>0.05) in clinical performance between any of the three adhesives after a period of two years.


2018 ◽  
Vol 43 (3) ◽  
pp. 261-271 ◽  
Author(s):  
M Demirci ◽  
S Tuncer ◽  
HS Sancakli ◽  
N Tekçe ◽  
C Baydemir

SUMMARY The purpose of this study was to evaluate a nanofilled and a nanohybrid composite, in combination with manufacturer-recommended etch-and-rinse adhesives, in class IV cavities. Thirty-four patients aged 14-46 years (mean age, 27.1 years) comprised the study group. Twenty-six patients received two class IV restorations and eight patients received four class IV restorations. For each patient, half the number of restorations were performed using a nanohybrid composite (Ceram X duo) and the remaining half used a nanofilled resin composite (Filtek Supreme XT), with two- (XP Bond) and three-step (Scotchbond Multipurpose) etch-and-rinse adhesives, respectively. Two experienced examiners evaluated the restorations for retention, color match, marginal discoloration, wear/loss of anatomic form, caries formation, marginal adaptation, and surface texture to compare the baseline (after placement) and annual recalls over 5 years. The cumulative success rates for the Filtek Supreme XT and Ceram X duo restorations after five years were 86.2% and 89.7%, respectively. Four Filtek Supreme XT and three Ceram X duo restorations failed. There was no statistically significant difference between the nanofilled and nanohybrid composites at any of the evaluation periods for any of the parameters evaluated. Despite the limited number of restorations, all restorations were clinically acceptable regarding retention, color match, marginal discoloration, wear or loss of anatomic form, the formation of caries, marginal adaptation, and surface texture, except the failed restorations. Fracture was the main cause of restoration failure.


2016 ◽  
Vol 41 (6) ◽  
pp. 599-606 ◽  
Author(s):  
F Öztürk-Bozkurt ◽  
T Toz ◽  
A Kara-Tuncer ◽  
H Gözükara-Bağ ◽  
M Özcan

SUMMARY In this study, the clinical performance of a silorane-based resin composite (SC) vs a nano-hybrid resin composite (NHC) was evaluated in Class II cavities. From January 2012 to February 2013, a total of 29 patients (eight men, 21 women; mean age, 24 ± 5 years) received 29 pairs of restorations using both SC (Filtek Silorane, 3M ESPE) and NHC (Filtek Z550, 3M ESPE) materials. Patients were followed until February 2015. One operator performed all restorations using the corresponding adhesive resins according to the manufacturers' instructions. Two calibrated independent examiners evaluated the restorations at one week, six months, and then annually using the modified United States Public Health Service (USPHS) criteria for anatomic form, marginal adaptation, color match, surface roughness, marginal discoloration, secondary caries, and postoperative sensitivity. Changes in the USPHS parameters were analyzed with the McNemar test (α=0.05). The mean observation period was 31.2 months. Marginal adaptation was the only parameter that showed a significant difference and was worse for SC than NHC (p=0.012). At the final recall, 17 restorations from the SC group and five from the NHC group received a score of 1 (explorer catches). These scores were significantly different between baseline and final recall for SC (p<0.001) but not for NHC (p>0.05). Both NHC and SC performed similarly in Class II restorations up to three years except for marginal adaptation, for which the latter demonstrated significant deterioration at the final recall compared with baseline.


2016 ◽  
Vol 41 (5) ◽  
pp. 520-530 ◽  
Author(s):  
T Suzuki ◽  
T Takamizawa ◽  
WW Barkmeier ◽  
A Tsujimoto ◽  
H Endo ◽  
...  

SUMMARY The purpose of this study was to determine the enamel bond durability of three universal adhesives in different etching modes through fatigue testing. The three universal adhesives used were Scotchbond Universal, Prime&Bond Elect universal dental adhesive, and All-Bond Universal light-cured dental adhesive. A single-step self-etch adhesive, Clearfil S3 Bond Plus was used as a control. The shear bond strength (SBS) and shear fatigue strength (SFS) to human enamel were evaluated in total-etch mode and self-etch mode. A stainless steel metal ring with an internal diameter of 2.4 mm was used to bond the resin composite to the flat-ground (4000-grit) tooth surfaces for determination of both SBS and SFS. For each enamel surface treatment, 15 specimens were prepared for SBS and 30 specimens for SFS. The staircase method for fatigue testing was then used to determine the SFS of the resin composite bonded to the enamel using 10-Hz frequencies for 50,000 cycles or until failure occurred. Scanning electron microscopy was used to observe representative debonded specimen surfaces and the resin-enamel interfaces. A two-way analysis of variance and the Tukey post hoc test were used for analysis of the SBS data, whereas a modified t-test with Bonferroni correction was used for the SFS data. All adhesives in total-etch mode showed significantly higher SBS and SFS values than those in self-etch mode. Although All-Bond Universal in self-etch mode showed a significantly lower SBS value than the other adhesives, there was no significant difference in SFS values among the adhesives in this mode. All adhesives showed higher SFS:SBS ratios in total-etch mode than in self-etch mode. With regard to the adhesive systems used in this study, universal adhesives showed higher enamel bond strengths in total-etch mode. Although the influence of different etching modes on the enamel-bonding performance of universal adhesives was found to be dependent on the adhesive material, total-etch mode effectively increased the enamel bond strength and durability, as measured by fatigue testing.


Author(s):  
N. Ranjith Kumar ◽  
G. Sheela Prakash ◽  
B.Vinay Goud

Introduction: Marginal adaptation is one of the most important and weakest links in the success of cast restorations. There is some indication that the quality of marginal adaptation may be as important to gingival health as marginal location.01 Aims and Objectives: To evaluate the marginal integrity and occlusal discrepancy of base metal alloy copings by recasting method using conventional casting technique. Methodology: The 30 test samples were grouped into 3groups as Group I: 10 samples will be prepared using 100% new co-cr alloy.Group II: 10 samples will be prepared using 50% new alloy and 50% reused alloy.Group III:10 samples will be prepared using 25% new alloy and 75% reused alloy. The patterns obtained were casted with an induction casting machine and Co-Cr copings, the vertical gap and internal gap/occlusal discrepancy of all the 30 Co-Cr cast copings are obtained, with the use of stereo microscope. Results: Paired t test and tukey Kramer tests were conducted to determine p value. The p value for marginal integrity  was found to be less than 0.01 which indicates that there is a significant difference between groups on buccal,lingual,mesial and distal surfaces . p value for occlusal discrepancy was found to be greater than 0.01 indicating that there is no significant  difference between groups on occlusal mesial surfaces while distal side it is less than 0.01 which is significant. Summary and Conclusion: The patterns were prepared with inlay casting wax on the custom-made stainless steel die. 30 of the patterns were invested with a metal ring during investment.  The vertical marginal gaps of three groups cast copings obtained by conventional casting were statistically significant to each other with higher value in group 2 followed by group 3 and group 1.


2017 ◽  
Vol 20 (4) ◽  
pp. 63 ◽  
Author(s):  
Raphaela Farias Rodrigues ◽  
Suellen Scarcelli Senna ◽  
Ana Flávia Soares ◽  
Rafael Lia Mondelli ◽  
Paulo Silveira Francisconi ◽  
...  

<p><strong>Objective</strong>: To evaluate the marginal adaptation, in enamel (E) and dentin (D), of composite resin (CR) associated with flowable resin composite (flow), bulk fill flowable base (bulk) and resin modified glass ionomer cement (RMGIC) in slot cavities. <strong>Material and Methods</strong>: The study was conducted after approval (Protocol No. 21148413.4.0000.5417) from Ethics Committee. Forty extracted human molar teeth were randomly assigned in eight experimental groups: E-CR, E-BULK, E-FLOW, E-RMGIC, D-CR, D-BULK, D-FLOW, D-RMGIC. The occlusal surface was planned, two slot cavities with standard sizes (depth: 2.0 mm, height: 2.5 mm, width: 2.0 mm) were created on a machine for making cavities. The teeth were restored and after 24h subjected to 2000 cyclic loading and sectioned for analysis of marginal adaptation by scanning electron microscopy (SEM). The micrographs were analyzed with the Image J program to measure the size of marginal gaps. The data were transformed into percentages (%GAPS = LG ÷ LM  × 100) and analyzed by 2-way ANOVA followed by the post hoc Tukey test (α=0.05). <strong>Results</strong>: There was a significant difference between different treatments (<em>p&lt;0.01</em>). The groups E-RMGIC (<em>p=0.001</em>) and D-RMGIC (<em>p=0</em>) had the highest percentage of marginal gap. Others groups showed similar percentage of marginal gap <em>(p&gt;0.05)</em>. <strong>Conclusions</strong>: It was concluded that restorations with flowable composite resin and bulk fill liners exhibit the same behavior, but the RMGIC liner increased marginal gap.</p><p><strong>Keywords: </strong>Composite resins; Dental marginal adaptation; Glass ionomer cements.</p>


2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Maryam Firouzmandi ◽  
Ali Asghar Alavi ◽  
Dana Jafarpour ◽  
Soroush Sadatsharifee

The aim of the present study was to compare the fracture strength and marginal adaptation of MOD cavities restored with Cention N, bonded Cention N, and resin composite, as well as to investigate the effect of cavity preparation volume on those properties. In this experimental study, 120 human maxillary premolars were randomly divided into six groups according to the type of restoration and cavity volume (n = 20): (I) conservative MOD restored with Cention N, (II) conservative MOD restored with bonded Cention N, (III) conservative MOD restored with Z250 resin composite, (IV) extended MOD restored with Cention N, (V) extended MOD restored with bonded Cention N, and (VI) extended MOD restored with Z250 resin composite. Fracture strength (MPa) was tested using a universal testing machine. To investigate marginal adaptation, polyvinyl-siloxane impressions were taken and poured with epoxy resin. Resin replicas were examined by SEM (×400) for marginal adaptation. ANOVA tests, Tukey’s test, and independent t-test were used to analyze data ( P  ≤ 0.05). Among conservative restorations, the fracture strength of bonded Cention N was significantly greater than that of Cention N ( P  = 0.001), while in the extended preparations, there was no significant difference between fracture strengths of different types of restorations ( P  = 0.579). In terms of marginal adaptation, there was no significant difference between different types of conservative restorations ( P  = 0.232). However, in extended preparations, composite showed significantly lower marginal adaptation than Cention N and bonded Cention N ( P  = 0.004 and P  = 0.045, respectively). Conservative preparations showed significantly greater fracture strength and marginal adaptation compared to extended ones in groups restored with composite. The volume of cavity preparation was shown to be effective in the materials fracture strength and marginal adaptation. Cention N showed promising results in terms of fracture strength and marginal adaptation.


2010 ◽  
Vol 2010 ◽  
pp. 1-9
Author(s):  
Tissiana Bortolotto ◽  
Carlo Monaco ◽  
Ioana Onisor ◽  
Ivo Krejci

The purpose of this paper was to investigate, by means of marginal adaptation and fracture strength, three different types of single retainer posterior fixed partial dentures (FPDs) for the replacement of a missing premolar. Two-unit cantilever FPDs were fabricated from composite resin, feldspathic porcelain, and fiber-reinforced composite resin. After luting procedures and margin polishing, all specimens were subjected to a Scanning Electron Microscopic marginal evaluation both prior to and after thermomechanical loading with a custom made chewing simulator comprising both thermal and mechanical loads. The results indicated that the highest score of marginal adaptation, that is, the closest score to 100% of continuous margins, at thetooth-composite resininterface was attained by the feldspathic porcelain group (88.1% median), followed by the fiber-reinforced composite resin group (78.9% median). The worse results were observed in the composite resin group (58.05% median). Fracture strength was higher in feldspathic porcelain (196N median) when compared to resin composite (114.9 N median). All the fixed prostheses made of fiber-reinforced composite resin detached from the abutment teeth before fracturing, suggesting that the adhesive surface's retainer should be increased.


2009 ◽  
Vol 10 (3) ◽  
pp. 18-26 ◽  
Author(s):  
Horieh Moosavi ◽  
Fathemeh Maleknejad ◽  
Nasrin Sarabi ◽  
Resa Shahriari ◽  
Taybeh Shayankhah

Abstract Aim The aim of this in vitro study was to evaluate the influence of application techniques (with pre-curing vs without pre-curing) for dentin adhesive on microleakage and marginal adaptation of indirect composite veneer restorations. Methods and Materials A total-etch bonding system, Excite/Variolink II (EXV), and a self-etching primer system, Panavia F2.0 (PF2), were used in the study. Forty-eight human central incisors were prepared for composite veneer restorations. The teeth were divided into two groups (n=24). For each resin cement, one half of each experimental group included an adhesive pre-cure (PC) with a halogen light source while the other half received no pre-cure (NPC) prior to resin cement insertion. Thus, four experimental groups were created: A (PC+EXV), B (NPC+EXV), C (PC+PF2), and D (NPC+PF2). Veneers made of Tetric Ceram resin composite were cemented using dual-cured resin luting agents. After storage in distilled water at 37°C for 24 hours, the teeth were prepared for marginal leakage. Two samples of each group were selected at random for scanning electron microscopic (SEM) observation and evaluation of marginal adaptation at 1050x magnification. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests (Ð <0.05). Results The highest and lowest microleakage values were observed in dentinal margins of groups B and A, respectively. Dentin margins opposite to enamel margins had a significant difference in microleakage values of PC and NPC groups (P<0.05). The influence of the adhesive pre-cure was more pronounced than the type of resin cement used. No adhesive layer was visualized for the adhesives used without employing the pre-curing step. Conclusion The effect of pre-cured adhesives was not material specific. The pre-cured adhesives showed the best resistance to dye penetration although the film thickness of these luting agents was only slightly increased. Clinical Significance Different curing methods (with pre-curing/without pre-curing) regardless of total-etch or self-etch adhesive systems influenced microleakage and the marginal adaptation, especially dentin margins of indirect composite veneers. Citation Maleknejad F, Moosavi H, Shahriari R, Sarabi S, Shayankhah T. The Effect of Different Adhesive Types and Curing Methods on Microleakage and the Marginal Adaptation of Composite Veneers. J Contemp Dent Pract 2009 May; (10)3:018-026.


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