scholarly journals Validation of a Screening Method Based on a Needle Stochastic Sensor for the Determination of Interleukins 1β, 6, and 12 in Biological Samples

Proceedings ◽  
2020 ◽  
Vol 55 (1) ◽  
pp. 14
Author(s):  
Sorin Sebastian Gheorghe ◽  
Ruxandra Maria Ilie-Mihai ◽  
Raluca-Ioana Stefan-van Staden ◽  
Alexandru Bratei
Keyword(s):  
Brain Cancer ◽  
Biological Samples ◽  
Whole Blood ◽  
Screening Method ◽  
Protoporphyrin Ix ◽  
Tissue Samples ◽  
Fast Screening ◽  
Relative Standard ◽  
Diamond Paste

Interleukins proved to be valuable biomarkers for different diseases. Interleukins 1β, 6, and 12 can be used as biomarkers for brain cancer diagnosis, and therefore this paper proposed a needle stochastic sensor based on protoporphyrin IX immobilized in nano-diamond paste for fast screening of biological samples, such as whole blood, urine and brain tumoral tissue, for these interleukins. The results obtained using this needle stochastic sensor proved that the interleukins 1β, 6, and 12 can be reliably determined from whole blood, urine and brain tumoral tissue, with recoveries higher than 96.00% and with relative standard deviations lower than 1.00%. The validation of the method was performed using whole blood and tissue samples collected from the patients confirmed with brain tumor.

scholarly journals Fast Screening of Whole Blood and Tumor Tissue for Bladder Cancer Biomarkers Using Stochastic Needle Sensors

Sensors ◽  
2020 ◽  
Vol 20 (8) ◽  
pp. 2420
Author(s):  
Raluca-Ioana Stefan-van Staden ◽  
Damaris-Cristina Gheorghe ◽  
Viorel Jinga ◽  
Cristian Sorin Sima ◽  
Marius Geanta
Keyword(s):  
Bladder Cancer ◽  
Hyaluronic Acid ◽  
Whole Blood ◽  
Screening Test ◽  
Tumor Tissue ◽  
Screening Tests ◽  
Tissue Samples ◽  
Fast Screening ◽  
Relative Standard ◽  
E Cadherin

Bladder cancer is one of the most common urologic malignancies, which is more frequent in men than in women. The early diagnosis for this type of cancer still remains a challenge, therefore, the development of a fast screening test for whole blood and tumor tissue samples may save lives. Four biomarkers, p53, E-cadherin, bladder tumor antigen (BTA), and hyaluronic acid were considered for the screening tests using stochastic needle sensors. Three stochastic needle sensors, based on graphite powder and modified with three types of chitosan, were designed and characterized for the screening test. The proposed sensors showed low limits of quantification, and high sensitivity and selectivity levels. The recoveries of p53, E-cadherin, BTA, and hyaluronic acid in whole blood samples and tissue samples were higher than 95.00% with a relative standard deviation lower than 1.00%.

Graphene-based stochastic sensors for pattern recognition of gastric cancer biomarkers in biological fluids

2019 ◽  
Vol 23 (11n12) ◽  
pp. 1365-1370 ◽  
Author(s):  
Raluca-Ioana Stefan-van Staden ◽  
Ruxandra-Maria Ilie-Mihai ◽  
Florina Pogacean ◽  
Stela Pruneanu
Keyword(s):  
Gastric Cancer ◽  
Pattern Recognition ◽  
Biological Samples ◽  
Whole Blood ◽  
Biological Fluids ◽  
Protoporphyrin Ix ◽  
Cancer Biomarkers ◽  
Exfoliated Graphene ◽  
Blood And Urine Samples

This paper proposes pattern recognition of gastric cancer biomarkers CEA, CA19-9 and p53 in whole blood and urine samples using a stochastic sensor based on exfoliated graphene (E-NGr) paste modified with protoporphyrin IX. The proposed sensor covered large ranges of concentrations: 1 × 10[Formula: see text]–1 × 10[Formula: see text]g/mL for CEA, 1 × 10[Formula: see text]–1 × 102 U/mL for CA19-9, and 0.2–5.0 [Formula: see text]g/mL for p53. These ranges allowed the determination of the three biomarkers from early to latest stages of gastric cancer. Validation of the pattern recognition of gastric cancer biomarkers was accomplished using biological samples: whole blood and urine.

scholarly journals Fast Screening Method of Biological Samples Based on Needle Stochastic Sensors for Early Detection of Gastric Cancer

2020 ◽  
Vol 72 (4) ◽  
pp. 22-34
Author(s):  
Ruxandra-Maria Ilie-Mihai ◽  
Damaris-Cristina Gheorghe ◽  
Raluca-Ioana Stefan-Van Staden ◽  
Alexandrina Lungu-Moscalu ◽  
Maria Coros ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Early Detection ◽  
Biological Samples ◽  
Early Detection Of Cancer ◽  
Tumor Tissue ◽  
Screening Method ◽  
Protoporphyrin Ix ◽  
Fast Screening ◽  
Doped Graphene ◽  
Linear Concentration

Early detection of cancer is essential for saving the life of patients. Needle stochastic sensors were proposed as new tools for the fast screening of biological samples. The design of the stochastic sensors was based on the immobilization of protoporphyrin IX in pastes of S-doped graphene. The selected biomarkers were CEA and CA19-9. High sensitivities, and wide linear concentration ranges as well as low limits of quantification were achieved. The proposed sensors were validated; student t-test shown that the proposed needle stochastic sensors can be reliably used for the pattern recognition and quantification of CEA and CA19-9 in whole blood, gastric tumor tissue, saliva, and urine.

scholarly journals Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Mohsen Keyvanfard ◽  
Khadijeh Alizad ◽  
Razieh Shakeri
Keyword(s):  
Standard Deviation ◽  
Sodium Cromoglycate ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Micellar Medium ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A new kinetic spectrophotometric method is described for the determination of ultratrace amounts of sodium cromoglycate (SCG). The method based on catalytic action of SCG on the oxidation of amaranth with periodate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of the amaranth at 518 nm, for the first 4 min from initiation of the reaction. Calibration curve was linear in the range of 4.0−36.0 ng mL−1SCG. The limit of detection is 2.7 ng mL−1SCG. The relative standard deviation (RSD) for ten replicate analyses of 12, 20, and 28 ng mL−1SCG was 0.40%, 0.32%, and 0.53%, respectively. The proposed method was used for the determination of SCG in biological samples.

scholarly journals Spectrophotometric Method for Determination of Paraquat in Food and Biological Samples

1997 ◽  
Vol 80 (2) ◽  
pp. 388-391 ◽  
Author(s):  
Ritu Kesari ◽  
Manish Rai ◽  
Vinay Kumar Gupta
Keyword(s):  
Standard Deviation ◽  
Metal Ions ◽  
Relative Standard Deviation ◽  
Alkaline Medium ◽  
Spectrophotometric Method ◽  
Biological Samples ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

Abstract A sensitive spectrophotometric method was developed for determination of paraquat, a widely used herbicide. Paraquat was reduced with glucose in an alkaline medium, and the blue radical ion obtained was measured at 600 nm. Beer’s law was obeyed at 0.1–1.2 ppm paraquat. The molar absorptivity was 1.26 × 105 L mol-1 cm-1. The standard deviation and relative standard deviation were ± 0.007 and 2.0%, respectively, for 5 μg paraquat/10 mL analyzed over 7 days. The method was free from interference by other commonly used pesticides and metal ions. The method may be used to the determine paraquat in plants, fruits, grains, water, blood, and urine.

Development of a robust, fast screening method for the potentiometric determination of iodide in urine and salt samples

Talanta ◽  
2017 ◽  
Vol 167 ◽  
pp. 688-694 ◽  
Author(s):  
Ana Machado ◽  
Raquel B.R. Mesquita ◽  
Sara Oliveira ◽  
Adriano A. Bordalo
Keyword(s):  
Screening Method ◽  
Potentiometric Determination ◽  
Fast Screening

Picomol Assay of Nitric Oxide Screening in Biological Samples by Derivatization Combined with High-Throughput Microplate Format

2013 ◽  
Vol 423-426 ◽  
pp. 1786-1789
Author(s):  
Xiao Ling Zhang ◽  
Qiao Yang
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
High Throughput ◽  
Biological Samples ◽  
Biological Sample ◽  
Small Volume ◽  
Screening Method ◽  
Broad Variety ◽  
Sensitive Determination

Nitric oxide (NO) is a critical intra-and extra-cellular signaling molecule that mediates a broad variety of physiologic and pathophysiologic events, and has prompted rapid growth of investigations in investigating its physiology and generation mechanism of corresponding diseases. In this paper, a picomol assay of NO screening method using fluorescent derivatization combined with high-throughout microplate format has been developed and successfully applied to the determination of NO in biological samples. The satisfied detection limit and recovery of the proposed method demonstrates that it can be competent for the sensitive determination of NO in extremely small volume of biological sample.

scholarly journals Sensitive Determination of Terazosin in Pharmaceutical Formulations and Biological Samples by Ionic-Liquid Microextraction Prior to Spectrofluorimetry

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Mohsen Zeeb ◽  
Mahdi Sadeghi
Keyword(s):  
Ionic Liquid ◽  
Ionic Strength ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Experimental Conditions ◽  
Relative Standard ◽  
Common Ion ◽  
Liquid Microextraction

An efficient and environmentally friendly sample preparation method based on the application of hydrophobic 1-Hexylpyridinium hexafluorophosphate [Hpy][PF6] ionic liquid (IL) as a microextraction solvent was proposed to preconcentrate terazosin. The performance of the microextraction method was improved by introducing a common ion of pyridinium IL into the sample solution. Due to the presence of the common ion, the solubility of IL significantly decreased. As a result, the phase separation successfully occurred even at high ionic strength, and the volume of the settled IL-phase was not influenced by variations in the ionic strength (up to 30% w/v). After preconcentration step, the enriched phase was introduced to the spectrofluorimeter for the determination of terazosin. The obtained results revealed that this system did not suffer from the limitations of that in conventional ionic-liquid microextraction. Under optimum experimental conditions, the proposed method provided a limit of detection (LOD) of 0.027 μg L−1and a relative standard deviation (R.S.D.) of 2.4%. The present method was successfully applied to terazosin determination in actual pharmaceutical formulations and biological samples. Considering the large variety of ionic liquids, the proposed microextraction method earns many merits, and will present a wide application in the future.

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