scholarly journals A Photoluminescent Colorimetric Probe of Bovine Serum Albumin-Stabilized Gold Nanoclusters for New Psychoactive Substances: Cathinone Drugs in Seized Street Samples

Sensors ◽  
2019 ◽  
Vol 19 (16) ◽  
pp. 3554 ◽  
Author(s):  
Yao-Te Yen ◽  
Ting-Yueh Chen ◽  
Chun-Yu Chen ◽  
Chi-Lun Chang ◽  
San-Chong Chyueh ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Illicit Drugs ◽  
Limit Of Detection ◽  
Gold Nanoclusters ◽  
New Psychoactive Substances ◽  
Psychoactive Substances ◽  
Electron Transfer Mechanism ◽  
Crime Scenes

Screening of illicit drugs for new psychoactive substances—namely cathinone—at crime scenes is in high demand. A dual-emission bovine serum albumin-stabilized gold nanoclusters probe was synthesized and used for quantitation and screening of 4-chloromethcathinone and cathinone analogues in an aqueous solution. The photoluminescent (PL) color of the bovine serum albumin-stabilized Au nanoclusters (BSA-Au NCs) probe solution changed from red to dark blue during the identification of cathinone drugs when excited using a portable ultraviolet light-emitting diodes lamp (365 nm). This probe solution allows the PL color-changing point and limit of detection down to 10.0 and 0.14 mM, respectively, for 4-chloromethcathinone. The phenomenon of PL color-changing of BSA-Au NCs was attributed to its PL band at 650 nm, quenching through an electron transfer mechanism. The probe solution was highly specific to cathinone drugs, over other popular illicit drugs, including heroin, cocaine, ketamine, and methamphetamine. The practicality of this BSA-Au NCs probe was assessed by using it to screen illicit drugs seized by law enforcement officers. All 20 actual cases from street and smuggling samples were validated using this BSA-Au NCs probe solution and then confirmed using gas chromatography–mass spectrometry. The results reveal this BSA-Au NCs probe solution is practical for screening cathinone drugs at crime scenes.

Deciphering the positional impact of chlorine in a new series of berberine analogues towards the superb-selective “turn-on” hydrophobic signaling of bovine serum albumin at physiological pH

2020 ◽  
Vol 44 (5) ◽  
pp. 1761-1771 ◽  
Author(s):  
Gopal Chandra Jana ◽  
Sk Nayim ◽  
Nandan Kumar Sahoo ◽  
Somnath Das ◽  
Mt Nasima Aktara ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Limit Of Detection ◽  
Selective Detection ◽  
Turn On

We report a new 9-O-benzyl substituted berberine analogue for the selective detection of BSA with a limit of detection value of 3.30 nM.

Radioimmunoassay for 13, 14-dihydro-15-ketoprostaglandin F2α and its application in normo- and anovulatory women

1982 ◽  
Vol 100 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Werner Schlegel ◽  
Jaime Urdinola ◽  
Hermann P. G. Schneider
Keyword(s):  
Menstrual Cycle ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Rapid Method ◽  
Plasma Levels ◽  
Bovine Serum ◽  
Limit Of Detection ◽  
Cross Reactivity ◽  
Specific Antibodies ◽  
Standard Range

Abstract. Highly specific antibodies to 13, 14-dihydro-15-ketoprostaglandin F2α (PGFM) were raised in rabbits. The animals were immunized with PGFM-bovine serum albumin (BSA)-conjugates. Prior to the incubation procedure PGFM was extracted by a rapid method with dichloromethane followed by column chromatography. The antisera dilution was 1:10000 and the cross-reactivity towards prostaglandin A2, E2, F2α, 13, 14-dihydro-15-ketoprostaglandin E2 and the 15-ketoprostaglandin E2 and F2α was < 1%. The limit of detection was 1.9 ± 0.6 pg/ml plasma over the standard range 1.9–250 pg. The intra- and inter-assay variations were 3.9 and 15%, respectively. PGFM was measured throughout the menstrual cycle in female volunteers. In normal ovulatory women (n = 3) plasma levels of PGFM varied between 65.6 to 107.1 pg/ml. No significant variations of plasma PGFM were seen during the cycle. In anovulatory women (n = 4) no difference of PGFM was found during the cycle. PGFM levels in hyperprolactinaemic but ovulating women tend to be higher than in anovulatory, and normoprolactinaemic subjects. These data strongly indicate that PGFM is not correlated with other hormonal parameters tested here in the normal and anovulatory cycles.

scholarly journals Identifying Reducing and Capping Sites of Protein-Encapsulated Gold Nanoclusters

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1630 ◽  
Author(s):  
Yu-Chen Hsu ◽  
Mei-Jou Hung ◽  
Yi-An Chen ◽  
Tsu-Fan Wang ◽  
Ying-Ru Ou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Conformational Changes ◽  
Local Structure ◽  
Bovine Serum ◽  
Gold Nanoclusters ◽  
Cysteic Acid ◽  
Subsequent Growth ◽  
The Core

The reducing and capping sites along with their local structure impact photo properties of the red bovine serum albumin-capped Au nanocluster (BSA-AuNC), however, they are hard to identify. We developped a workflow and relevant techniques using mass spectrometry (MS) to identify the reducing and capping sites of BSA-AuNCs involved in their formation and fluorescence. Digestion without disulfide cleavages yielded an Au core fraction exhibiting red fluorescence and [AunSm] ion signals and a non-core fraction exhibiting neither of them. The core fraction was identified to mainly be comprised of peptides containing cysteine residues. The fluorescence and [AunSm] signals were quenched by tris(2-carboxyethyl)phosphine, confirming that disulfide groups were required for nanocluster stabilization and fluorescence. By MS sequencing, the disulfide pairs, C75–C91/C90–C101 in domain IA, C315–C360/C359–C368 in domain IIB, and C513–C558/C557–C566 in domain IIIB, were identified to be main capping sites of red AuNCs. Peptides containing oxidized cysteines (sulfinic or cysteic acid) were identified as reducing sites mainly in the non-core fraction, suggesting that disulfide cleavages by oxidization and conformational changes contributed to the subsequent growth of nanoclusters at nearby intact disulfide pairs. This is the first report on precise identification of the reducing and capping sites of BSA-AuNCs.

scholarly journals A Turn-On Fluorescent Sensor for Glutathione Based on Bovine Serum Albumin-Stabilized Gold Nanoclusters

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Yan Qiu ◽  
Jianlin Huang ◽  
Li Jia
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fluorescent Sensor ◽  
Gold Nanoclusters ◽  
Copper Ion ◽  
Fluorescence Sensor ◽  
Gold Nanocluster ◽  
Experimental Conditions ◽  
Bovine Serum Albumin Molecule

A fluorescence sensor for the detection of glutathione based on the fluorescence recovering of the bovine serum albumin-stabilized gold nanoclusters is reported. This study indicates that glutathione restores the copper-ion-quenched fluorescence by coordinating the bound copper ion in the bovine serum albumin molecule used for stabilizing the gold nanocluster through its sulfydryl. Under the experimental conditions, the fluorescence response showed a linear relationship with the concentration of glutathione over the range from 10 µM to 400 µM. The fluorescence sensor successfully detected glutathione in commercial drug products.

scholarly journals Bovine Serum Albumin Protein-Based Liquid Crystal Biosensors for Optical Detection of Toxic Heavy Metals in Water

Sensors ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 298 ◽  
Author(s):  
Noor ul Amin ◽  
Humaira Masood Siddiqi ◽  
Yang Kun Lin ◽  
Zakir Hussain ◽  
Nasir Majeed
Keyword(s):  
Heavy Metal ◽  
Liquid Crystal ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Metal Ions ◽  
Bovine Serum ◽  
Heavy Metal Ions ◽  
Limit Of Detection ◽  
Optical Image ◽  
Toxic Heavy Metals

A new methodology involving the use of Bovine Serum Albumin (BSA) as a probe and liquid crystal (LC) as a signal reporter for the detection of heavy metal ions in water at neutral pH was developed. BSA acted as a multi-dentate ligand for the detection of multiple metal ions. The LC sensor was fabricated by immobilizing 3 µg mL−1 BSA solution on dimethyloctadecyl-[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP)-coated glass slides. In the absence of heavy metal ions, a dark optical image was observed, while in their presence, a dark optical image turned to bright. The optical response was characterized by using a polarized optical microscope (POM). The BSA based LC sensor selectively detected toxic metal ions as compared to s block metal ions and ammonium ions in water. Moreover, the limit of detection was found to be very low (i.e., 1 nM) for the developed new biosensor in comparison to reported biosensors.

Ratiometric fluorescence determination of alkaline phosphatase activity based on dual emission of bovine serum albumin-stabilized gold nanoclusters and the inner filter effect

The Analyst ◽  
2021 ◽  
Author(s):  
Li Pu ◽  
Mengfan Xia ◽  
Pengyue Sun ◽  
Yaodong Zhang
Keyword(s):  
Alkaline Phosphatase ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Gold Nanoclusters ◽  
Inner Filter Effect ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Filter Effect

Ratiometric fluorescence assay of alkaline phosphatase based on dual emission of bovine serum albumin-templated gold nanoclusters and inner filter effect.

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