Fluorometric Optimized Determination of Total Glutathione in Erythrocytes

Glutathione is a tripeptide natural product characterized by a non-canonical peptide bond with an amide moiety linking the nitrogen of cysteine to the γ-carboxyl of glutamate, and is found ubiquitously in nature, in animals, plants and microorganisms. One of the most abundant biological matrices is represented by erythrocytes, being glutathione the only sulfur-containing mechanism for the red blood cell oxidative protection. Several analytical methods for glutathione determination from different samples are described in the literature and most of these methods are based on the use of high-performance liquid chromatography. HPLC equipment is not available in all the biochemical laboratories, and, moreover, displays lot of economic and ecological limitations, including organic solvent consumption and time-consuming analysis. Here, an organic-free high-throughput fluorometric methodology for the analysis of total glutathione in erythrocytes is reported, avoiding the use of time-consuming and not-sustainable techniques.

A ratiometric fluorescence platform composed of MnO2 nanosheets and nitrogen, chlorine co-doped carbon dots and its logic gate performance for glutathione determination

Herein, a ratiometric fluorescence strategy based on the MnO2 nanosheets and nitrogen, chlorine co-doped carbon dots (N, Cl-CDs) was constructed for selective determination glutathione (GSH) and the absolute fluorescence quantum...

A turn-on fluorescence strategy for cellular glutathione determination based on the aggregation-induced emission enhancement of self-assembled copper nanoclusters

A turn-on fluorescence method has been designed for the determination of cellular GSH levels based on the controlled aggregation-induced emission enhancement of self-assembled CuNCs.

Voltammetric method for determination of glutathione on a gold-carbon-containing electrode

Glutathione (GSH) is one of the most important thiol-containing antioxidants involved into various biochemical processes in the human body. Glutathione determination in biological fluids (saliva, urine, serum) and pharmaceutical preparations is rather important for clinical practice. Various analytical methods — spectrophotometry, fluorimetry, high-performance liquid chromatography, NMR spectroscopy, capillary electrophoresis and electrochemical methods — are widely used for this purpose. Electrochemical methods are characterized by easy implementation, low cost and possibility of miniaturization. The electrochemical behavior of reduced (GSH) and oxidized (GSSG) glutathione on a gold-carbon-containing electrode (AuCE) was studied using cathodic voltammetry with different methods of removing oxygen from an electrochemical cell: nitrogen sparging and addition of sodium sulfite (4 mol/dm3). It has been shown that traces of H2O2 that remain in the near-electrode layer on the AuCE even after oxygen removal influence the electrochemical properties of GSH at a cathode sweep of the potential from 0 to –1.8 V: GSH is oxidized by H2O2 to GSSG, the most important product of this reaction is O2. An indirect determination of GSH by the current of oxygen reduction in the Na2SO3 medium in the concentration range from 0.5 × 10–8 to 4.2 × 10–8 mol/dm3 with a detection limit of 2.5 × 10–9 mol/dm3 is proposed. The developed voltammetric method is approved for the determination of GSH in certain pharmaceutical preparations.

Dual role of BSA for synthesis of MnO2nanoparticles and their mediated fluorescent turn-on probe for glutathione determination and cancer cell recognition

Scheme of the MnO2NP-mediated fluorescence turn-off-on process of CDs with MnO2NPs and GSH.