scholarly journals Development and Application of Molecularly Imprinted Polymers for the Selective Extraction of Chlordecone from Bovine Serum

Separations ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 237
Author(s):  
Pauline Bosman ◽  
Audrey Combès ◽  
Marine Lambert ◽  
Gwenaëlle Lavison-Bompard ◽  
Valérie Pichon
Keyword(s):  
Bovine Serum ◽  
Solid Phase ◽  
Extraction Procedure ◽  
Selective Extraction ◽  
Human Consumption ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Synthesis Conditions ◽  
Selective Retention ◽  
Bovine Serum Sample

The widespread use of chlordecone (CLD), an organochlorine pesticide, until the 1990s to protect banana crops in the French West Indies led to significant pollution of water and soil and, subsequently, of bovine intended for human consumption. Carcasses are submitted to official controls based on perirenal fat CLD determination. In order to allow for pre-slaughter controls, a selective analytical method based on a molecularly imprinted polymer (MIP) associated to the LC/MS-MS method was developed to determine the level of CLD in bovine serum that can be collected before slaughter. Different synthesis conditions were therefore assayed by varying the nature of the monomer and of the porogen, and the most promising MIP in terms of selective retention for CLD (extraction recovery close to 100%) was completely characterized by solid-phase extraction (repeatability of the extraction procedure, of the synthesis, and of the cartridge filling) in pure medium. The capacity of the MIP was determined at 0.13 µmol g−1 of MIP. After application of a spiked bovine serum sample on the MIP, the selective retention was maintained (87 and 21%, respectively, on the MIP and on the corresponding non-imprinted polymer). Moreover, extraction on the MIP led to a cleaner extract compared to those issued from a conventional C18 sorbent.

scholarly journals Performance Evaluation of Molecularly Imprinted Polymer using Propanol as Porogen for Atenolol Recognition in Human Serum

2018 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Meilia Suherman ◽  
Ike Susanti ◽  
Driyanti Rahayu ◽  
Rimadani Pratiwi ◽  
Aliya Nur Hasanah
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Solid Phase ◽  
Therapeutic Index ◽  
Selective Extraction ◽  
Bulk Polymerization ◽  
Precipitation Polymerization ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
As Doping ◽  
Recovery Percentage

Atenolol is a cardiovascular drug that has a narrow therapeutic index with long-term use and it’s often used as doping. Atenolol has a small concentration in human boby and it’s in  biological matrix (serum) so in the testing need a selective extraction so  the analyte can be pra-concentration and removed from matrix. Two molecularly imprinted polymers (MIPs) on propanol as porogen  have been made with two different methods i.e. bulk polymerization and precipitation polymerization. The polymer was made using atenolol as a template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a crosslinker. Prformance evaluations showed that polymers from bulk polymerization provide better performance than polymers from precipitation polymerization when tested against standard solution. However, this sorbent has low  recovery percentage after applied into serum sample and could not be used as alternative for atenolol extraction in human serum.Key words: Molecularly imprinted polymer, Atenolol, Solid Phase Extraction, Preparation  method, propanol.

scholarly journals A graphene oxide surface–molecularly imprinted polymer as a dispersive solid-phase extraction adsorbent for the determination of cefadroxil in water samples

RSC Advances ◽  
2017 ◽  
Vol 7 (54) ◽  
pp. 34077-34085 ◽  
Author(s):  
Xinwei Chen ◽  
Nengsheng Ye
Keyword(s):  
Graphene Oxide ◽  
Molecularly Imprinted Polymer ◽  
Water Samples ◽  
Solid Phase ◽  
Environmental Water ◽  
Selective Extraction ◽  
Oxide Surface ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

Graphene oxide–functionalized molecularly imprinted polymer particles have been prepared for specific selective extraction and determination of cefadroxil in environmental water samples.

scholarly journals Preparation and utilization of a molecularly imprinted polymer for solid phase extraction of tramadol

2010 ◽  
Vol 8 (4) ◽  
pp. 861-869 ◽  
Author(s):  
Saman Azodi-Deilami ◽  
Majid Abdouss ◽  
Seyed Hasani
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Molecularly Imprinted Polymer ◽  
Solid Phase ◽  
Extraction Procedure ◽  
Molecular Structures ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Human Plasma And Urine

AbstractIn this paper, a highly selective molecularly imprinted polymer (MIP) for tramadol hydrochloride, a drug used to treat moderate to severe pain, was prepared and its use as solid-phase extraction (SPE) sorbent was demonstrated. The molecularly imprinted solid-phase extraction procedure followed by high performance liquid chromatography with ultraviolet detector (MISPE-HPLC) was developed for selective extraction and determination of tramadol in human plasma and urine. The optimal conditions for molecularly imprinted solid-phase extraction (MISPE) consisted of conditioning with 1 mL methanol and 1 mL of deionized water at neutral pH, loading of tramadol sample (50 µg L−1) at pH 7.5, washing using 1 mL acetone and elution with 3 × 1 mL of 10% (v/v) acetic acid in methanol. The MIP selectivity was evaluated by checking several substances with similar molecular structures to that of tramadol. Results from the HPLC analyses showed that the calibration curve of tramadol (using MIP from human plasma and urine) is linear in the ranges of 6–100 and 3–120 µg L−1 with good precisions (1.9% and 2.9% for 5.0 µg L−1), respectively. The recoveries for plasma and urine samples were higher than 81%.

scholarly journals Molecularly Imprinted Polymers as Solid-Phase Microextraction Fibers for the Isolation of Selected Antibiotics from Human Plasma

Materials ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 4886
Author(s):  
Małgorzata Szultka-Młyńska ◽  
Daria Janiszewska ◽  
Bogusław Buszewski
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Solid Phase Microextraction ◽  
High Performance ◽  
Solid Phase ◽  
Selective Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Antibiotic Drugs ◽  
Central Composite ◽  
As Solid Phase

The aim of this study was to examine the synthesis of novel molecularly imprinted polymer (MIP)-coated polythiophene and poly(3-methylthiophene) solid-phase microextraction fibers using the direct electropolymerization method. Synthesized SPME fibers were characterized with the use of various physicochemical instrumental techniques. MIP-SPME coatings were successfully applied to carry out the selective extraction of selected antibiotic drugs (amoxicillin, cefotaxime, metronidazole) and their metabolites (amoxycilloic acid, amoxicillin diketopiperazine, desacetyl cefotaxime, 3-desacetyl cefotaxime lactone, hydroxymetronidazole). Solid-phase microextraction parameters for the simultaneous determination and identification of target compounds were optimized using the central composite design (CCD), and they accounted for 5–15 min for desorption time, 3–10 for the pH of the desorption solvent, and 30–100 μL for the volume of the desorption solvent. High-performance liquid chromatography and mass spectrometry (MS) detectors such as quadrupole time-of-flight (Q-TOF MS) and triple quadrupole (QqQ MS) were applied to determine and to identify selected antibiotic drugs and their metabolites. The MIP-coated SPME are suitable for the selective extraction of target compounds in biological samples from patients in intensive care units.

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