scholarly journals Isolation and Purification of a Hydrophobic Non-Ribosomal Peptide from an Escherichia coli Fermentation Broth

Separations ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 241
Author(s):  
Arne Michael Oestreich ◽  
Sebastian Reinhardt ◽  
Doreen Gerlach ◽  
Rong Fan ◽  
Peter Czermak
Keyword(s):  
High Performance ◽  
Fermentation Broth ◽  
Chemical Properties ◽  
Fold Increase ◽  
Complete Removal ◽  
Downstream Process ◽  
Isolation And Purification ◽  
Unit Operations ◽  
Physical And Chemical ◽  
And Chemical Properties

Non-ribosomal peptide synthases (NRPSs) generate versatile bioactive peptides by incorporating non-proteinogenic amino acids and catalyzing diverse modifications. Here, we developed an efficient downstream process for the capture, intermediate purification and polishing of a rhabdopeptide (RXP) produced by the NRPS VietABC. Many typical unit operations were unsuitable due to the similar physical and chemical properties of the RXP and related byproducts. However, we were able to capture the RXP from a fermentation broth using a hydrophobic resin (XAD-16N), resulting in a 14-fold increase in concentration while removing salts as well as polar and weak non-polar impurities. We then used ultra-high-performance liquid chromatography (UHPLC) for intermediate purification, with optimized parameters determined using statistical experimental designs, resulting in the complete removal of hydrophobic impurities. Finally, the UHPLC eluents were removed by evaporation. Our three-step downstream process achieved an overall product recovery of 81.7 ± 8.4%.

Tuning Packing, Structural Flexibility, and Porosity in 2D Metal–Organic Frameworks by Metal Node Choice

2019 ◽  
Vol 72 (10) ◽  
pp. 797 ◽  
Author(s):  
Witold M. Bloch ◽  
Christian J. Doonan ◽  
Christopher J. Sumby
Keyword(s):  
Structural Changes ◽  
Metal Organic Frameworks ◽  
Chemical Properties ◽  
Complete Removal ◽  
Structural Flexibility ◽  
X Ray Diffraction ◽  
Metal Centre ◽  
Metal Organic ◽  
Physical And Chemical ◽  
And Chemical Properties

Understanding the key features that determine structural flexibility in metal–organic frameworks (MOFs) is key to exploiting their dynamic physical and chemical properties. We have previously reported a 2D MOF material, CuL1, comprising five-coordinate metal nodes that displays exceptional CO2/N2 selectively (L1=bis(4-(4-carboxyphenyl)-1H-pyrazolyl)methane). Here we examine the effect of utilising six-coordinate metal centres (CoII and NiII) in the synthesis of isostructural MOFs from L1, namely CoL1 and NiL1. The octahedral geometry of the metal centre within the MOF analogues precludes an ideal eclipse of the 2D layers, resulting in an offset stacking, and in certain cases, the formation of 2-fold interpenetrated analogues β-CoL1 and β-NiL1. We used a combination of thermogravimetric analysis (TGA), and powder and single crystal X-ray diffraction (PXRD and SCXRD) to show that desolvation is accompanied by a structural change for NiL1, and complete removal of the coordinated H2O ligands results in a reduction in long-range order. The offset nature of the 2D layers in combination with the structural changes impedes the adsorption of meaningful quantities of gases (N2, CO2), highlighting the importance of a five-coordinate metal centre in achieving optimal pore accessibility for this family of flexible materials.

Effect of Doping of Fe into TiO2 Layer in Fe2O3/TiO2/FTO System for High Performance of Water Splitting

2014 ◽  
Vol 93 ◽  
pp. 82-89 ◽  
Author(s):  
Aryan Azad ◽  
Eul Noh ◽  
Kang Seop Yun ◽  
Hee June Jeong ◽  
Sang Chul Jung ◽  
...  
Keyword(s):  
Metal Oxides ◽  
Water Splitting ◽  
High Performance ◽  
Chemical Properties ◽  
Layer By Layer ◽  
Energy Problem ◽  
Dipping Process ◽  
Promising Solution ◽  
Physical And Chemical ◽  
And Chemical Properties

Hydrogen has unique physical and chemical properties which present benefits and challenges to its successful widespread adoption as a fuel. The photoelectrochemical (PEC) water splitting process with semiconductor metal oxides can be a promising solution to the global energy problem. Although amongst metal oxides Fe2O3 by 2.2 eV bang gap energy is more applicable, for reducing the recombination of electron and hole, Fe was doped into TiO2. In this study Fe2O3/Fe doped TiO2 photocatalysts were compared with Fe-doped TiO2 and TiO2 structures by using layer by layer-self-assemble (LBL-SA) method and dipping process on FTO glass. According to our results the Fe2O3 coated on Fe doped TiO2 /FTO has had best results.

High performance graphene embedded rubber composites

RSC Advances ◽  
2015 ◽  
Vol 5 (99) ◽  
pp. 81707-81712 ◽  
Author(s):  
Sung Ho Song ◽  
Jung Mo Kim ◽  
Kwang Hyun Park ◽  
Dong Ju Lee ◽  
O.-Seok Kwon ◽  
...  
Keyword(s):  
High Performance ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Rubber Composites ◽  
Synergy Effects ◽  
Physical And Chemical ◽  
And Chemical Properties

Nano-fillers have provided a big advantage for enhancing the performance of rubber composites through leading the synergy effects in the physical and chemical properties.

Metal Organic Framework Derived Porous Carbon Materials Excels as an Excellent Platform for High-performance Packaged Supercapacitors

Nanoscale ◽  
2021 ◽  
Author(s):  
Xianyu Chu ◽  
Fanling Meng ◽  
Ting Deng ◽  
Wei Zhang
Keyword(s):  
Porous Carbon ◽  
High Performance ◽  
Metal Organic Framework ◽  
Chemical Properties ◽  
Porous Carbon Material ◽  
Metal Organic ◽  
Key Steps ◽  
Physical And Chemical ◽  
And Chemical Properties ◽  
Organic Framework

Designing and synthesizing new materials with special physical and chemical properties are the key steps to assembling high performance supercapacitors. Metal organic framework (MOF) derived porous carbon material has drawn...

scholarly journals Research of the physical and chemical properties and methods of Red rice (food colour) determination in sausage products

2020 ◽  
Vol 5 (4) ◽  
pp. 23-28
Author(s):  
N. V. Rudometova ◽  
I. S. Kim
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Chemical Properties ◽  
Carminic Acid ◽  
Physical And Chemical Properties ◽  
Red Rice ◽  
Chromatography Method ◽  
Food Colour ◽  
Physical And Chemical ◽  
And Chemical Properties

In Russia, in the production of meat and sausage products, the food colour, named as Red rice, is use. Red rice is obtain by the cultivation the strains of the Monascus fungus on various carbohydrate substrates, for example rice. That Red rice may contain the mycotoxin citrinin, but neither the purity of the food colour nor the safety profile are regulated. The aim of this work was to study the physical and chemical properties of Red rice and to develop method for its determination in sausage products. The experiments were carried out on model and commercial samples of sausages. The samples were analyzed using spectrophotometry and highefficiency planar and liquid chromatography. Spectrophotometric analysis revealed differences in solubility, coloring power, spectral characteristics, composition and ratio of pigments in commercial samples of Red rice. The extraction parameters of colouring substances from sausages model samples were determined. It has been established that Red rice is extracted with chloroform, acetone, ethanol and its aqueous solutions. Petroleum ether was proposed for degreasing sausages. Red rice from sausages was extracted with acetone because it did not extract synthetic colours and carminic acid. It was found, that 90% of Red rice is extracted from samples of sausages by double extraction with ultrasonic treatment with a power of 128 W. High performance thin layer chromatography method and high performance liquid chromatography method for the Red rice identification was proposed. The content of Red rice in the extracts was determined by spectrophotometric method. The developed method for the determination of Red rice was tested on commercial samples of sausage products.

scholarly journals Wafer-scale two-dimensional platinum monosulfide ultrathin film via metal sulfurization for high performance photoelectronics

2022 ◽  
Author(s):  
Jinbo Pang ◽  
Yanhao Wang ◽  
Xiaoxin Yang ◽  
Lei Zhang ◽  
Yufen Li ◽  
...  
Keyword(s):  
High Performance ◽  
Chemical Properties ◽  
Ultrathin Film ◽  
Physical And Chemical Properties ◽  
Two Dimensional ◽  
Wafer Scale ◽  
Platinum Group Minerals ◽  
Platinum Group ◽  
Physical And Chemical ◽  
And Chemical Properties

2D nonlayered materials have attracted enormous research interests due to their novel physical and chemical properties with the confined dimensions. Platinum sulfide as one of the most common platinum-group minerals...

The Research on Preparation of a New Building Material with EMR and their Properties

2010 ◽  
Vol 163-167 ◽  
pp. 4575-4579 ◽  
Author(s):  
Yong Wang ◽  
Wen Hao Ye ◽  
Heng Bo Liu
Keyword(s):  
Building Material ◽  
High Performance ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Siliceous Material ◽  
Main Components ◽  
Physical And Chemical ◽  
And Chemical Properties

This article describes the physical and chemical properties of EMR and the preparation of a new building material with it. The results show that the main components of EMR are gypsum and quartz, supplemented by a small amount of cement, appropriate siliceous material and quicklime in EMR could be prepared high performance EMR autoclaved brick.

Application of Castor Oil-Based Cutting Fluids in Precision Turning

2011 ◽  
Vol 130-134 ◽  
pp. 3830-3834
Author(s):  
Hong Jie Pei ◽  
Y.J. Shen ◽  
Chun Gen Shen ◽  
Gui Cheng Wang
Keyword(s):  
Development Strategy ◽  
Castor Oil ◽  
High Performance ◽  
Chemical Properties ◽  
National Standards ◽  
Cutting Fluid ◽  
Price Ratio ◽  
Physical And Chemical ◽  
And Chemical Properties ◽  
Lubricating Properties

Green machining is a process that implements sustainable development strategy. In order to reduce cost and decrease environmental pollution in manufacturing process, a sort of biodegradable castor oil-based emulsion is developed, whose physical and chemical properties can correspond with the national standards. The cooling and lubricating properties of the emulsion are better while machining steel materials through comparative experiments with certain imported commodity synthetic cutting fluid. The emulsion can successfully substitute for commodity fluid because of its high performance-price ratio.

scholarly journals SELECTION OF MINERAL RAW MATERIAL FIELDS FOR GLAUCONITE SORPTION MATERIALS

2021 ◽  
Vol 2021 (1) ◽  
pp. 11-18
Author(s):  
D Xadjibaev ◽  
◽  
F Erkabaev
Keyword(s):  
Industrial Development ◽  
High Performance ◽  
Raw Materials ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Sorption Process ◽  
Raw Material ◽  
Physical And Chemical Properties ◽  
Physical And Chemical ◽  
And Chemical Properties

Natural minerals, such as bentonite, kaolinite, biotite, vermiculite, and glauconite, which have sufficiently high performance properties in the softening of industrial and waste water, softening and cleaning of heavy metals, are promising. To study the physical and chemical properties of glauconites from various deposits of the country, samples of glauconite mineral were brought from the Parkent, Bukhara, and Karakalpak mountain reserves, their physical and chemical properties were studied, compared with each other, and the optimal reserve was selected. The reserves of these deposits are sufficient and have the potential for industrial development. The purpose of this study is to compare samples of natural mineral reserves of glauconite and select the optimal deposit for raw materials. Methodology. Samples of glauconite minerals from the Parkent, Bukhara and Karakalpak mines were taken as the object of the study. Chemical, mineralogical, and thermal analyses were performed on the samples, and the fractional composition was determined. Scientific novelty. The main part of the mineral glauconite (50-55%) in the raw material is found in fractions of 0.08-0.125 mm. The most suitable raw material was the Parkent glauconite deposit, which has a relatively high content of potassium, which tends to exchange with other metals during the sorption process. The received data. Based on the conducted experiments, samples of glauconites from Parkent, Bukhara, and Karakalpak were subjected to chemical and mineralogical analyses. The maximum amount of the mineral glauconite was found in the fractions of the raw material (less than 0.125 mm). The most suitable deposit of raw materials for processing the mineral glauconite was selected and an environmentally friendly adsorbent was obtained.

Effect of a temporally heterogeneous environment on size and shape of the giant ostracods Mytilocypris (Ostracoda : Cyprididae) from Australian salt lakes

2004 ◽  
Vol 55 (5) ◽  
pp. 499 ◽  
Author(s):  
Terrie L. Finston
Keyword(s):  
Environmental Changes ◽  
High Salinity ◽  
Chemical Properties ◽  
Fold Increase ◽  
Sympatric Species ◽  
Physical And Chemical Properties ◽  
Saline Habitats ◽  
Successive Generations ◽  
Physical And Chemical ◽  
And Chemical Properties

The giant ostracods Mytilocypris occupy temporary saline habitats in Australia and experience physical and chemical heterogeneity in association with evaporation. Three sympatric species, namely M. mytiloides, M. tasmanica chapmani, and M. minuta, show high genetic similarity and morphological boundaries that overlap. The large elongate, intermediate, and small blunt shells of the three species are associated with lakes of low, intermediate, and high salinity, respectively. The present study investigated whether all three phenotypes were produced through time in single habitats experiencing seasonal changes in physical and chemical properties. Three lakes at one site (Coorow) were temporally heterogeneous, showing a nearly 10-fold increase in salinity in a single season, whereas four lakes at a second site (Rottnest Island) were more or less permanent and showed less environmental variation. Successive generations in the lakes from Coorow became smaller and blunter in association with environmental changes, showing the range of phenotypes displayed in the three presumptive taxa. There was less phenotypic response in individuals from Rottnest Island, reflecting the relative homogeneity of the lakes. Possible mechanisms underlying the observed changes include phenotypic plasticity, genotype replacement, and strong selection.

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