scholarly journals Membrane Purification Techniques for Recovery of Succinic Acid Obtained from Fermentation Broth during Bioconversion of Lignocellulosic Biomass: Current Advances and Future Perspectives

2021 ◽  
Vol 13 (12) ◽  
pp. 6794
Author(s):  
Olawumi O. Sadare ◽  
Olayile Ejekwu ◽  
Moloko F. Moshokoa ◽  
Monsurat O. Jimoh ◽  
Michael O. Daramola
Keyword(s):  
Succinic Acid ◽  
Lignocellulosic Biomass ◽  
Fermentation Broth ◽  
Short Review ◽  
Downstream Processing ◽  
Critical Issue ◽  
Industrial Applications ◽  
Global Market ◽  
Green Technologies ◽  
One Pot

Recently, the bioconversion of biomass into biofuels and biocommodities has received significant attention. Although green technologies for biofuel and biocommodity production are advancing, the productivity and yield from these techniques are low. Over the past years, various recovery and purification techniques have been developed and successfully employed to improve these technologies. However, these technologies still require improvement regarding the energy-consumption-related costs, low yield and product purity. In the context of sustainable green production, this review presents a broad review of membrane purification technologies/methods for succinic acid, a biocommodity obtained from lignocellulosic biomass. In addition, a short overview of the global market for sustainable green chemistry and circular economy systems or zero waste approach towards a sustainable waste management is presented. Succinic acid, the available feedstocks for its production and its industrial applications are also highlighted. Downstream separation processes of succinic acid and the current studies on different downstream processing techniques are critically reviewed. Furthermore, critical analysis of membrane-based downstream processes of succinic acid production from fermentation broth is highlighted. A short review of the integrated-membrane-based process is discussed, as well, because integrating “one-pot” lignocellulosic bioconversion to succinic acid with downstream separation processing is considered a critical issue to address. In conclusion, speculations on outlook are suggested.

scholarly journals Metabolic and Process Engineering for Microbial Production of Protocatechuate from Xylose with Corynebacterium glutamicum

2021 ◽  
Author(s):  
Mohamed Labib ◽  
Jonas Görtz ◽  
Christian Brüsseler ◽  
Nicolai Kallscheuer ◽  
Jochem Gätgens ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Fermentation Broth ◽  
Downstream Processing ◽  
Microbial Production ◽  
One Pot ◽  
Theoretical Yield ◽  
Maximum Titer ◽  
Hydroxybenzoic Acids ◽  
Cultivation Process ◽  
Wide Potential

3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA is of interest in the chemical industry as a building block for novel polymers and has wide potential for pharmaceutical applications due to its antioxidant, anti-inflammatory, and antiviral properties. In the present study, we designed and constructed a novel Corynebacterium glutamicum strain to enable the efficient utilization of d-xylose for microbial production of PCA. The engineered strain showed a maximum PCA titer of 62.1 ± 12.1 mM (9.6 ± 1.9 g L−1) from d-xylose as the primary carbon and energy source. The corresponding yield was , which corresponds to 38 % of the maximum theoretical yield and is 14-fold higher compared to the parental producer strain on d-glucose. By establishing a one-pot bioreactor cultivation process followed by subsequent process optimization, the same maximum titer and a total amount of 16.5 ± 1.1 g was reached. Downstream processing of PCA from this fermentation broth was realized via electrochemically induced crystallization by taking advantage of the pH-dependent properties of PCA. Since PCA turned out to be electrochemically unstable in combination with several anode materials, a threechamber electrolysis setup was established to crystallize PCA and to avoid direct anode contact. This resulted in a maximum final purity of 95.4 %. In summary, the established PCA production process represents a highly sustainable approach, which will serve as a blueprint for the bio-based production of other hydroxybenzoic acids from alternative sugar feedstocks.

scholarly journals A highly thermotolerant laccase produced by Cerrena unicolor strain CGMCC 5.1011 for complete and stable malachite green decolorization

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanhua Yao ◽  
Guimei Zhou ◽  
Yonghui Lin ◽  
Xinqi Xu ◽  
Jie Yang
Keyword(s):  
Malachite Green ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Life Time ◽  
Industrial Applications ◽  
Dynamics Simulation ◽  
Carbon And Nitrogen ◽  
Cdna Sequences ◽  
Cerrena Unicolor ◽  
Future Work

Abstract Laccases are a class of multi-copper oxidases with important industrial values. A thermotolerant laccase produced by a basidiomycete fungal strain Cerrena unicolor CGMCC 5.1011 was studied. With glycerin and peptone as the carbon and nitrogen sources, respectively, a maximal laccase activity of 121.7 U/mL was attained after cultivation in the shaking flask for 15 days. Transcriptomics analysis revealed an expressed laccase gene family of 12 members in C. unicolor strain CGMCC 5.1011, and the gene and cDNA sequences were cloned. A glycosylated laccase was purified from the fermentation broth of Cerrena unicolor CGMCC 5.1011 and corresponded to Lac2 based on MALDI-TOF MS/MS identification. Lac2 was stable at pH 5.0 and above, and was resistant to organic solvents. Lac2 displayed remarkable thermostability, with half-life time of 1.67 h at 70 ºC. Consistently, Lac2 was able to completely decolorize malachite green (MG) at high temperatures, whereas Lac7 from Cerrena sp. HYB07 resulted in accumulation of colored MG transformation intermediates. Molecular dynamics simulation of Lac2 was conducted, and possible mechanisms underlying Lac2 thermostability were discussed. The robustness of C. unicolor CGMCC 5.1011 laccase would not only be useful for industrial applications, but also provide a template for future work to develop thermostable laccases.

scholarly journals Recent Findings in Azaphilone Pigments

2021 ◽  
Vol 7 (7) ◽  
pp. 541
Author(s):  
Lúcia P. S. Pimenta ◽  
Dhionne C. Gomes ◽  
Patrícia G. Cardoso ◽  
Jacqueline A. Takahashi
Keyword(s):  
Water Solubility ◽  
Biological Activities ◽  
Low Cost ◽  
Scale Up ◽  
Structural Diversity ◽  
Downstream Processing ◽  
Global Market ◽  
Yield Improvement ◽  
Potential Applications ◽  
New Compounds

Filamentous fungi are known to biosynthesize an extraordinary range of azaphilones pigments with structural diversity and advantages over vegetal-derived colored natural products such agile and simple cultivation in the lab, acceptance of low-cost substrates, speed yield improvement, and ease of downstream processing. Modern genetic engineering allows industrial production, providing pigments with higher thermostability, water-solubility, and promising bioactivities combined with ecological functions. This review, covering the literature from 2020 onwards, focuses on the state-of-the-art of azaphilone dyes, the global market scenario, new compounds isolated in the period with respective biological activities, and biosynthetic pathways. Furthermore, we discussed the innovations of azaphilone cultivation and extraction techniques, as well as in yield improvement and scale-up. Potential applications in the food, cosmetic, pharmaceutical, and textile industries were also explored.

scholarly journals Purification of High Value Succinic Acid from Biomass Fermentation Broth Via Nanofiltration

2016 ◽  
Vol 9 (21) ◽  
Author(s):  
Nadiah Khairul Zaman ◽  
Rosiah Rohani ◽  
Muhammad Hanis Abdul Shukor ◽  
Abdul Wahab Mohamad
Keyword(s):  
Succinic Acid ◽  
Fermentation Broth

MODIFICATION OF POLYBUTADIENE RUBBER: A REVIEW

2021 ◽  
Author(s):  
Parashiva Prabhu C. ◽  
Subhra Mohanty ◽  
Virendra Kumar Gupta
Keyword(s):  
Chain Length ◽  
Industrial Applications ◽  
Global Market ◽  
Specific Method ◽  
Reactivity Ratios ◽  
Reaction Conditions ◽  
Commercial Products ◽  
Polybutadiene Rubber ◽  
Metal Catalyzed

ABSTRACT Developments in modification of polybutadiene rubber (PBR) using various reagents and catalysts have been reviewed. Hydrogenation and functionalization occurring at the site of unsaturation along chain length are discussed. Hydrogenation involving various metal catalyzed processes is discussed. Suitable conditions that are effective during hydrogenation and functionalization are mentioned in this article. Reactivity ratios associated with microstructures of polybutadiene rubber and possible mechanisms involved are described in the review. The importance of reaction conditions during reactivity and their impact on product properties are highlighted. A specific method that needs to be adopted in order to achieve expected product properties is discussed. Various industrial applications of modified PBR and their commercial products in the global market are discussed.

scholarly journals Industrial applications of laser-induced breakdown spectroscopy: a review

2020 ◽  
Vol 12 (8) ◽  
pp. 1014-1029 ◽  
Author(s):  
S. Legnaioli ◽  
B. Campanella ◽  
F. Poggialini ◽  
S. Pagnotta ◽  
M. A. Harith ◽  
...  
Keyword(s):  
Short Review ◽  
Industrial Applications ◽  
Laser Induced Breakdown Spectroscopy ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown

A short review on LIBS industrial applications.

Development of vegetable oil-based emulsion liquid membrane for downstream processing of bio-succinic acid

2020 ◽  
Vol 119 ◽  
pp. 161-169 ◽  
Author(s):  
Norela Jusoh ◽  
Raja Norimie Raja Sulaiman ◽  
Norasikin Othman ◽  
Norul Fatiha Mohamed Noah ◽  
Muhammad Bukhari Rosly ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Vegetable Oil ◽  
Liquid Membrane ◽  
Downstream Processing ◽  
Emulsion Liquid Membrane
Sign in / Sign up

Export Citation Format

Share Document