The Toxicological Intersection between Allergen and Toxin: A Structural Comparison of the Cat Dander Allergenic Protein Fel d1 and the Slow Loris Brachial Gland Secretion Protein

Slow lorises are enigmatic animal that represent the only venomous primate lineage. Their defensive secretions have received little attention. In this study we determined the full length sequence of the protein secreted by their unique brachial glands. The full length sequences displayed homology to the main allergenic protein present in cat dander. We thus compared the molecular features of the slow loris brachial gland protein and the cat dander allergen protein, showing remarkable similarities between them. Thus we postulate that allergenic proteins play a role in the slow loris defensive arsenal. These results shed light on these neglected, novel animals.

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Relationship between Phenolic Compounds, Antioxidant Properties, and the Allergenic Protein Mal d 1 in Different Selenium-Biofortified Apple Cultivars (Malus domestica)

Molecules ◽

10.3390/molecules26092647 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2647

Author(s):

Sabrina Groth ◽

Christoph Budke ◽

Timo Weber ◽

Susanne Neugart ◽

Sven Brockmann ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Properties ◽

Total Phenolic ◽

Allergenic Protein ◽

Antibody Recognition ◽

Allergenic Potential ◽

Allergenic Proteins ◽

Other Substances ◽

Mal D 1 ◽

Apple Cultivars

Notable parts of the population in Europe suffer from allergies towards apples. To address this health problem, the analysis of the interactions of relevant allergens with other substances such as phenolic compounds is of particular importance. The aim of this study was to evaluate the correlations between the total phenolic content (TPC), polyphenol oxidase (PPO) activity, antioxidant activity (AOA), and the phenolic compound profile and the content of the allergenic protein Mal d 1 in six apple cultivars. It was found that the PPO activity and the content of individual phenolic compounds had an influence on the Mal d 1 content. With regard to the important constituents, flavan-3-ols and phenolic acids, it was found that apples with a higher content of chlorogenic acid and a low content of procyanidin trimers and/or epicatechin had a lower allergenic potential. This is probably based on the reaction of phenolic compounds (when oxidized by the endogenous PPO) with proteins, thus being able to change the conformation of the (allergenic) proteins, which further corresponds to a loss of antibody recognition. When apples were additionally biofortified with selenium, the composition of the apples, with regard to TPC, phenolic profile, AOA, and PPO, was significantly affected. Consequently, this innovative agronomic practice seems to be promising for reducing the allergenic potential of apples.

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The genomic full‐length sequence of the HLA‐A *24:257 allele, identified by full‐length group‐specific sequencing

HLA ◽

10.1111/tan.14424 ◽

2021 ◽

Author(s):

Bin Hu ◽

Hihui Feng ◽

Shuxian Jiao ◽

Shutao Pang

Keyword(s):

Full Length ◽

Length Group ◽

Full Length Sequence

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Genomic full‐length sequence of the HLA‐B*52:23 allele was identified by full length group‐specific sequencing

HLA ◽

10.1111/tan.14435 ◽

2021 ◽

Author(s):

Zhihui Feng ◽

Shuxian Jiao ◽

Bin Hu ◽

Shutao Pang

Keyword(s):

Full Length ◽

Length Group ◽

Full Length Sequence ◽

B 52

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Isolation and full-length sequence analysis of Armigeres subalbatus totivirus, the first totivirus isolate from mosquitoes representing a proposed novel genus (Artivirus) of the family Totiviridae

Journal of General Virology ◽

10.1099/vir.0.024794-0 ◽

2010 ◽

Vol 91 (11) ◽

pp. 2836-2845 ◽

Cited By ~ 53

Author(s):

Y. Zhai ◽

H. Attoui ◽

F. Mohd Jaafar ◽

H.-q. Wang ◽

Y.-x. Cao ◽

...

Keyword(s):

Sequence Analysis ◽

Full Length ◽

Novel Genus ◽

Armigeres Subalbatus ◽

The Family ◽

Full Length Sequence

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Full-length sequence of a novel HLA-B*15:220 allele identified in an individual from Guadeloupe

Tissue Antigens ◽

10.1111/j.1399-0039.2011.01762.x ◽

2011 ◽

Vol 79 (1) ◽

pp. 75-76 ◽

Cited By ~ 5

Author(s):

M. O. R. F. Joannes ◽

C. E. M. Voorter ◽

F. Agis ◽

M. G. J. Tilanus

Keyword(s):

Full Length ◽

Full Length Sequence

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Full-length sequence and genome analysis of CHV1-CN280, a North China isolate of cryphonectria hypovirus 1

Archives of Virology ◽

10.1007/s00705-017-3296-2 ◽

2017 ◽

Vol 162 (6) ◽

pp. 1811-1818 ◽

Cited By ~ 4

Author(s):

Yanan Du ◽

Yuan Lin ◽

Xuan Zhou ◽

Kerong Wang ◽

Shouguo Fang ◽

...

Keyword(s):

Genome Analysis ◽

North China ◽

Full Length ◽

Full Length Sequence ◽

Cryphonectria Hypovirus

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BMP-binding modules in chordin: a model for signalling regulation in the extracellular space

Development ◽

10.1242/dev.127.4.821 ◽

2000 ◽

Vol 127 (4) ◽

pp. 821-830 ◽

Cited By ~ 10

Author(s):

J. Larrain ◽

D. Bachiller ◽

B. Lu ◽

E. Agius ◽

S. Piccolo ◽

...

Keyword(s):

Dissociation Constants ◽

Extracellular Proteins ◽

Full Length ◽

Dorsoventral Patterning ◽

C Elegans ◽

Growth Factor Signalling ◽

Bmp Antagonist ◽

Nanomolar Range ◽

Shed Light ◽

Novel Protein

A number of genetic and molecular studies have implicated Chordin in the regulation of dorsoventral patterning during gastrulation. Chordin, a BMP antagonist of 120 kDa, contains four small (about 70 amino acids each) cysteine-rich domains (CRs) of unknown function. In this study, we show that the Chordin CRs define a novel protein module for the binding and regulation of BMPs. The biological activity of Chordin resides in the CRs, especially in CR1 and CR3, which have dorsalizing activity in Xenopus embryo assays and bind BMP4 with dissociation constants in the nanomolar range. The activity of individual CRs, however, is 5- to 10-fold lower than that of full-length Chordin. These results shed light on the molecular mechanism by which Chordin/BMP complexes are regulated by the metalloprotease Xolloid, which cleaves in the vicinity of CR1 and CR3 and would release CR/BMP complexes with lower anti-BMP activity than intact Chordin. CR domains are found in other extracellular proteins such as procollagens. Full-length Xenopus procollagen IIA mRNA has dorsalizing activity in embryo microinjection assays and the CR domain is required for this activity. Similarly, a C. elegans cDNA containing five CR domains induces secondary axes in injected Xenopus embryos. These results suggest that CR modules may function in a number of extracellular proteins to regulate growth factor signalling.

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Creative Process II

Dave Brubeck's Time Out ◽

10.1093/oso/9780190217716.003.0005 ◽

2019 ◽

pp. 117-136

Author(s):

Stephen A. Crist

Keyword(s):

Creative Process ◽

Full Length ◽

Time Out ◽

Audio Recordings ◽

History Of ◽

Shed Light ◽

Surprising Discovery ◽

The Way

Among Dave Brubeck’s personal audio recordings are about two dozen takes of “Take Five,” which preceded the full-length version on Time Out and the shorter single release version. This chapter discusses first the familiar versions from July 1959, and then considers how the Quartet’s efforts at the end of June paved the way for those iconic recordings. A surprising discovery from the original session recordings of “Kathy’s Waltz” and “Strange Meadow Lark” is that both cuts were spliced from supplemental takes. The recording history of “Everybody’s Jumpin’ ” includes the unexpected fact that a portion of the tune originated in 1950, nine years before the Time Out sessions. The chapter also examines the original recordings of “Three to Get Ready” and “Blue Rondo à la Turk,” as well as two tracks that shed light on the origins of “Pick Up Sticks.”

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