scholarly journals Effect of Different Species of Prorocentrum Genus on the Japanese Oyster Crassostrea gigas Proteomic Profile

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 504
Author(s):  
Miguel Angel Matus Hernández ◽  
Norma Yolanda Hernández Saavedra
Keyword(s):  
Crassostrea Gigas ◽  
Developmental Stages ◽  
Prorocentrum Minimum ◽  
Solid Base ◽  
Proteomic Profile ◽  
Whole Cells ◽  
Japanese Oyster ◽  
Toxic Concentrations ◽  
Analytical Tools ◽  
Main Effects

This paper assesses the effects of exposure to toxic concentrations (1200 to 6000 cells/mL) of the dinoflagellates Prorocentrum lima, Prorocentrum minimum, and Prorocentrum rhathymum and several concentrations of aqueous and organic extracts obtained from the same species (0 to 20 parts per thousand) on the Crassostrea gigas (5–7 mm) proteomic profile. Through comparative proteomic map analyses, several protein spots were detected with different expression levels, of which eight were selected to be identified by liquid chromatography–mass spectrometry (LC–MS/MS) analyses. The proteomic response suggests that, after 72 h of exposure to whole cells, the biological functions of C. gigas affected proteins in the immune system, stress response, contractile systems and cytoskeletal activities. The exposure to organic and aqueous extracts mainly showed effects on protein expressions in muscle contraction and cytoskeleton morphology. These results enrich the knowledge on early bivalve developmental stages. Therefore, they may be considered a solid base for new bioassays and/or generation of specific analytical tools that allow for some of the main effects of algal proliferation phenomena on bivalve mollusk development to be monitored, characterized and elucidated.

Development of Haemoglobin by De-embryonated Chick Blastoderms Cultured in vitro and the Effect of Abnormal RNA upon its Synthesis

Development ◽  
1961 ◽  
Vol 9 (1) ◽  
pp. 202-221
Author(s):  
B. R. A. O'Brien
Keyword(s):  
Protein Synthesis ◽  
Developmental Stages ◽  
Specific Protein ◽  
Cytoplasmic Protein ◽  
Whole Cells ◽  
Restricted Group ◽  
Dividing Cells ◽  
First Time ◽  
Structural Code

The embryo provides a sequence of developmental stages in which proteins both structural and enzymatic appear or become detectable for the first time in a restricted group of dividing cells. The cells or tissues can be maintained in vitro for a period that may precede and include the synthesis of a specific ‘cytoplasmic’ protein. In this way systems of protein synthesis within the cells of higher organisms can be studied during those stages in which current hypotheses suggest that some structural code is passed on from the DNA of the nucleus to the cytoplasm where the synthesis of the protein becomes maximal. Acellular preparations have contributed much to the elucidation of protein synthesis, but it is doubtful whether actual net synthesis has been obtained in systems less complex than the ‘protoplast’ developed by Spiegelman (1957). In order to study the synthesis of a specific protein it seems necessary at this stage to use whole cells.

Pathological changes by Eosinophilic Rickettsia-like organism in Japanese oyster, Crassostrea gigas

2019 ◽  
Vol 167 ◽  
pp. 107248
Author(s):  
Liliana Carvalho-Saucedo ◽  
Ilie S. Racotta ◽  
Citlali Guerra-Danielsen
Keyword(s):  
Crassostrea Gigas ◽  
Pathological Changes ◽  
Japanese Oyster

A population dynamics model for the Japanese oyster, Crassostrea gigas

Aquaculture ◽  
1997 ◽  
Vol 149 (3-4) ◽  
pp. 285-321 ◽  
Author(s):  
Masato Kobayashi ◽  
Eileen E Hofmann ◽  
Eric N Powell ◽  
John M Klinck ◽  
Koji Kusaka
Keyword(s):  
Population Dynamics ◽  
Crassostrea Gigas ◽  
Dynamics Model ◽  
Population Dynamics Model ◽  
Japanese Oyster

Cryopreservation of spermatozoa of the Japanese oyster, Crassostrea gigas

Aquaculture ◽  
1986 ◽  
Vol 58 (3-4) ◽  
pp. 277-280 ◽  
Author(s):  
S. Bougrier ◽  
L.D. Rabenomanana
Keyword(s):  
Crassostrea Gigas ◽  
Japanese Oyster

Overdominance of a Muscle Protein (Mp-1) Locus in the Japanese Oyster, Crassostrea gigas (Ostreidae)

1979 ◽  
Vol 36 (11) ◽  
pp. 1313-1318 ◽  
Author(s):  
N. E. Buroker
Keyword(s):  
Marine Environment ◽  
Crassostrea Gigas ◽  
Genetic Study ◽  
Thermal Gradient ◽  
Population Genetic ◽  
Muscle Protein ◽  
Selective Advantage ◽  
Relative Fitness ◽  
Population Genetic Study ◽  
Japanese Oyster

A microgeographical population genetic study of the Japanese oyster, Crassostrea gigas, revealed a case of overdominance for a muscle protein (Mp-1) locus. This locus consists of a two allele polymorphism. A study of the relative "fitness" of the three genotypes (Mp-1105/105, Mp-1105/100, and Mp-1100/100) revealed a heterozygote superiority in the segment of the oyster population at the lowest tidal level (+ 1.2 m). In segments of the population at higher tidal heights (i.e. + 1.8 and + 2.4 m), there appears to be a selective advantage for Mp-1105 allele since the highest "fitness" values belong to the Mp-1105/105 genotype. Since oysters do not appear at the field station lower than 1.2 tidal m, it could not be determined if the Mp-1100 allele had a selective advantage at tidal levels lower than 1.2 m. However, the Mp-1105 allele possibly exhibits a selective advantage in the subtropical marine environment of southern Japan, while the Mp-1100 allele is favored in the temperate marine environment of northern Japan. This suggests perhaps that a micro- and macrogeographical cline in gene frequency exists for the Mp-1 locus due to a thermal gradient. Key words: Japanese oyster, Crassostrea gigas, population genetic study, overdominance, heterozygote superiority, Mp-1 locus, micro- and macrogeographical cline, thermal gradient, tidal height, latitude

scholarly journals In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement

2019 ◽  
Vol 20 (1) ◽  
pp. 197 ◽  
Author(s):  
Valentin Foulon ◽  
Pierre Boudry ◽  
Sébastien Artigaud ◽  
Fabienne Guérard ◽  
Claire Hellio
Keyword(s):  
Crassostrea Gigas ◽  
In Silico ◽  
Pacific Oyster ◽  
Developmental Stages ◽  
Larval Settlement ◽  
In Silico Analysis ◽  
Suitable Habitat ◽  
Related Proteins ◽  
Von Willebrand ◽  
Silico Analysis

Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae.

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