scholarly journals A Single-Nucleotide Polymorphism of αVβ3 Integrin Is Associated with the Andes Virus Infection Susceptibility

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 169 ◽  
Author(s):  
Constanza Martínez-Valdebenito ◽  
Jenniffer Angulo ◽  
Nicole Le Corre ◽  
Claudia Marco ◽  
Cecilia Vial ◽  
...  
Keyword(s):  
Αvβ3 Integrin ◽  
Risk Of Infection ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Household Contacts ◽  
The Andes ◽  
Andes Virus ◽  
Infection Susceptibility ◽  
Human Polymorphism ◽  
Host Genetic

The Andes Orthohantavirus (ANDV), which causes the hantavirus cardiopulmonary syndrome, enters cells via integrins, and a change from leucine to proline at residue 33 in the PSI domain (L33P), impairs ANDV recognition. We assessed the association between this human polymorphism and ANDV infection. We defined susceptible and protective genotypes as “TT” (coding leucine) and “CC” (coding proline), respectively. TT was present at a rate of 89.2% (66/74) among the first cohort of ANDV cases and at 60% (63/105) among exposed close-household contacts, who remained uninfected (p < 0.05). The protective genotype (CC) was absent in all 85 ANDV cases, in both cohorts, and was present at 11.4% of the exposed close-household contacts who remained uninfected. Logistic regression modeling for risk of infection had an OR of 6.2–12.6 (p < 0.05) in the presence of TT and well-known ANDV risk activities. Moreover, an OR of 7.3 was obtained when the TT condition was analyzed for two groups exposed to the same environmental risk. Host genetic background was found to have an important role in ANDV infection susceptibility, in the studied population.

scholarly journals Single nucleotide variant at the alphaVbeta3 integrin associated to Andes virus infection susceptibility.

2018 ◽  
Author(s):  
Constanza Martinez-Valdebenito ◽  
Jenniffer Angulo ◽  
N. Le Corre ◽  
Clauda Marco ◽  
Cecilia Vial ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Genetic Background ◽  
Protein A ◽  
Cell Protein ◽  
Susceptible Genotype ◽  
Single Nucleotide ◽  
Household Contacts ◽  
Andes Virus ◽  
Infection Susceptibility ◽  
Host Genetic

Background ANDV, agent of hantavirus cardiopulmonary syndrome, enters through integrin cell protein. A change from leucine-to-proline at residues 33 in the PSI-domain (L33P) inhibits ANDV recognition. We assessed the association between this human-variant and ANDV infection. Results We defined susceptible genotype to “TT” (coding leucine) and protective “CC” (coding proline). TT was in 89.2% (66/74) of a first cohort of ANDV-cases and in 60% (63/105) of exposed close-household contacts who remained unifected (p<0.05). Protective genotype was absent in all 85 ANDV cases in both cohorts and was present at 11.4% in exposed close-household contacts who remained uninfected. Logistic regression modeling to become a case had an OR 6.2-12.6 (p<0.05) in presence of TT and ANDV well-known risk activities. Moreover, OR of 7.3 was obtained when TT condition was analyzed for two groups exposed to the same environmental risk. Conclusion Host genetic background has an important role in ANDV-infection susceptibility in the studied population.

scholarly journals 2502. Host Susceptibility to Andes Hantavirus Infection Associates to a Single Nucleotide Polymorphism at the αVβ3 Integrin

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S751-S751
Author(s):  
Constanza Martinez-Valdebenito ◽  
Marcela Ferres ◽  
Nicole Le Corre ◽  
Jenniffer Angulo ◽  
Cecilia Vial ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Host Susceptibility ◽  
Hantavirus Infection ◽  
Αvβ3 Integrin ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Andes Hantavirus

scholarly journals Modeling of variables in cellular infection reveals CXCL10 levels are regulated by human genetic variation and the Chlamydia-encoded CPAF protease

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Benjamin H. Schott ◽  
Alejandro L. Antonia ◽  
Liuyang Wang ◽  
Kelly J. Pittman ◽  
Barbara S. Sixt ◽  
...  
Keyword(s):  
T Cell ◽  
Cultured Cells ◽  
Nucleotide Polymorphism ◽  
Linear Modeling ◽  
Strong Negative Correlation ◽  
Single Nucleotide ◽  
Obligate Intracellular ◽  
Activity Factor ◽  
Host Genetic ◽  
Infection Parameters

Abstract Susceptibility to infectious diseases is determined by a complex interaction between host and pathogen. For infections with the obligate intracellular bacterium Chlamydia trachomatis, variation in immune activation and disease presentation are regulated by both host genetic diversity and pathogen immune evasion. Previously, we discovered a single nucleotide polymorphism (rs2869462) associated with absolute abundance of CXCL10, a pro-inflammatory T-cell chemokine. Here, we report that levels of CXCL10 change during C. trachomatis infection of cultured cells in a manner dependent on both host and pathogen. Linear modeling of cellular traits associated with CXCL10 levels identified a strong, negative correlation with bacterial burden, suggesting that C. trachomatis actively suppresses CXCL10. We identified the pathogen-encoded factor responsible for this suppression as the chlamydial protease- or proteasome-like activity factor, CPAF. Further, we applied our modeling approach to other host cytokines in response to C. trachomatis and found evidence that RANTES, another T-cell chemoattractant, is actively suppressed by Chlamydia. However, this observed suppression of RANTES is not mediated by CPAF. Overall, our results demonstrate that CPAF suppresses CXCL10 to evade the host cytokine response and that modeling of cellular infection parameters can reveal previously unrecognized facets of host–pathogen interactions.

UTILIZATION OF DNA MARKERS FOR EVALUATING AMYLOSE CONTENT OF GRAIN IN RICE

2013 ◽  
Vol 83 (5) ◽  
Author(s):  
Dương Thanh Thủy ◽  
Taiichiro Ookawa
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Amylose Content ◽  
Waxy Gene ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Branching Enzymes ◽  
Wx Gene ◽  
High Amylose ◽  
Suitable Marker ◽  
Granule Bound Starch Synthase

The sensory and functional properties of rice are predominantly associated with its amylose content. Granule-bound starch synthase (GBSS) encoded by the Waxy (Wx) gene determines the synthesis of amylose, while starch branching enzymes encoded by Sbe genes are involved in the formation of amylopectin. Some studies have demonstrated that Wx gene is the major controller of amylose content but there are one or more modifying genes affecting the amylose content. Three markers,  microsatellite, Single – nucleotide – polymorphism (G/T SNP) in Wx gene and Single – nucleotide – polymorphism (T/C SNP) in Sbe1 gene, were tested for their association with amylose content using sixty-nine  rice accessions from twenty countries. Of the three markers, two markers in Wx gene are significantly associated with amylose content. The combination of two markers in Wx gene (haplotypes) explained 83.8% of the variation in amylose content and discriminated the three market classes of glutinous, low, intermediate and high amylose content of rice from each other. And T/C SNP in Sbe1 locus was not a suitable marker for amylose content. Keywords: marker, amylose content, Waxy gene.

Sign in / Sign up

Export Citation Format

Share Document