DETERMINATION OF SHORT CHAIN FATTY ACIDS IN RAT FECES TREATED WITH RESISTANT STARCH BY HPLC

2019 ◽  
pp. 65-72
Author(s):  
Huu Tien Nguyen ◽  
Thi Mai Khanh Nguyen ◽  
Huu Dung Tran
Keyword(s):  
Fatty Acids ◽  
Resistant Starch ◽  
Anaerobic Fermentation ◽  
Living Organism ◽  
Short Chain Fatty Acids ◽  
Hplc Method ◽  
Wheat Starch ◽  
Short Chain ◽  
Us Fda ◽  
Chain Fatty Acids

Background: Short-chain fatty acids (SCFAs), mostly found in colon feces, is an important group of gut microbial metabolites from anaerobic fermentation of indigestible carbohydrates. Objectives: To develope an HPLC method to determined SCFAs in rat feces treated with resistant starch. Materials and methods: Sample is the rat feces fed with acetate wheat starch and normal starch; fatty acid hydrazides are derived from SCFAs in feces and measured by HPLC. After validated as guidance of US-FDA, method is applied to identify SCFAs in rat treated two types of starch. Results: the HPLC condition was optimized as follow: Eclipse XDB–C8 (4.6mmx150mm, 5µm) column; mobile phase: methanol, acetonitrile and 0,057 mM acid triflouroacetic (pH 4.5) (0:13:87 – 10:20:70 – 0:13:87, v/v/v) in 40 mins;, examinized wavelength: 396 nm. Method was validated with parameters: system suitability, specificity, linearity, precision, accuracy and stability. The result showed amount ratio of SCFAs in feces of mice group treated with acetate wheat starch containing resistant starch higher than from the diet containing normal starch significantly. Conclusion: This method can be used to investigate SCFAs in the gastrointestinal segments of the living organism. Key words: SCFAs, HPLC, resistant starch, feces

scholarly journals Short-Chain Fatty Acids and Their Association with Signalling Pathways in Inflammation, Glucose and Lipid Metabolism

2020 ◽  
Vol 21 (17) ◽  
pp. 6356 ◽  
Author(s):  
Jin He ◽  
Peiwen Zhang ◽  
Linyuan Shen ◽  
Lili Niu ◽  
Ya Tan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lipid Metabolism ◽  
Histone Deacetylases ◽  
Anaerobic Fermentation ◽  
Short Chain Fatty Acids ◽  
Biological Properties ◽  
Short Chain ◽  
Signalling Pathways ◽  
Glucose And Lipid Metabolism ◽  
Chain Fatty Acids

Short-chain fatty acids (SCFAs), particularly acetate, propionate and butyrate, are mainly produced by anaerobic fermentation of gut microbes. SCFAs play an important role in regulating energy metabolism and energy supply, as well as maintaining the homeostasis of the intestinal environment. In recent years, many studies have shown that SCFAs demonstrate physiologically beneficial effects, and the signalling pathways related to SCFA production, absorption, metabolism, and intestinal effects have been discovered. Two major signalling pathways concerning SCFAs, G-protein-coupled receptors (GPRCs) and histone deacetylases (HDACs), are well recognized. In this review, we summarize the recent advances concerning the biological properties of SCFAs and the signalling pathways in inflammation and glucose and lipid metabolism.

Short-Chain Fatty Acids and Human Colonic Function: Roles of Resistant Starch and Nonstarch Polysaccharides

2001 ◽  
Vol 81 (3) ◽  
pp. 1031-1064 ◽  
Author(s):  
David L. Topping ◽  
Peter M. Clifton
Keyword(s):  
Fatty Acids ◽  
Resistant Starch ◽  
Epidemiological Data ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Indirect Measures ◽  
Electrolyte Uptake ◽  
Colonic Blood Flow ◽  
Nonstarch Polysaccharides ◽  
Chain Fatty Acids

Resistant starch (RS) is starch and products of its small intestinal digestion that enter the large bowel. It occurs for various reasons including chemical structure, cooking of food, chemical modification, and food mastication. Human colonic bacteria ferment RS and nonstarch polysaccharides (NSP; major components of dietary fiber) to short-chain fatty acids (SCFA), mainly acetate, propionate, and butyrate. SCFA stimulate colonic blood flow and fluid and electrolyte uptake. Butyrate is a preferred substrate for colonocytes and appears to promote a normal phenotype in these cells. Fermentation of some RS types favors butyrate production. Measurement of colonic fermentation in humans is difficult, and indirect measures (e.g., fecal samples) or animal models have been used. Of the latter, rodents appear to be of limited value, and pigs or dogs are preferable. RS is less effective than NSP in stool bulking, but epidemiological data suggest that it is more protective against colorectal cancer, possibly via butyrate. RS is a prebiotic, but knowledge of its other interactions with the microflora is limited. The contribution of RS to fermentation and colonic physiology seems to be greater than that of NSP. However, the lack of a generally accepted analytical procedure that accommodates the major influences on RS means this is yet to be established.

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