Faculty Opinions recommendation of Ultrasensitive Immunoprofiling of Plasma Extracellular Vesicles Identifies Syndecan-1 as a Potential Tool for Minimally Invasive Diagnosis of Glioma.

2020 ◽  
Author(s):  
Nikos Karamanos ◽  
Zoi Piperigkou
Keyword(s):  
Minimally Invasive ◽  
Extracellular Vesicles ◽  
Potential Tool

Ultrasensitive Immunoprofiling of Plasma Extracellular Vesicles Identifies Syndecan-1 as a Potential Tool for Minimally Invasive Diagnosis of Glioma

2019 ◽  
Vol 25 (10) ◽  
pp. 3115-3127 ◽  
Author(s):  
Vineesh Indira Chandran ◽  
Charlotte Welinder ◽  
Ann-Sofie Månsson ◽  
Svenja Offer ◽  
Eva Freyhult ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Extracellular Vesicles ◽  
Potential Tool

scholarly journals Genomic instability-derived plasma extracellular vesicle-microRNA signature as a minimally invasive predictor of risk and unfavorable prognosis in breast cancer

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Siqi Bao ◽  
Ting Hu ◽  
Jiaqi Liu ◽  
Jianzhong Su ◽  
Jie Sun ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Early Diagnosis ◽  
Minimally Invasive ◽  
Genomic Instability ◽  
Extracellular Vesicles ◽  
Disease Risk ◽  
Prognostic Indicator ◽  
Mirna Regulation ◽  
Mirna Signature ◽  
Operating Characteristics

Abstract Background Breast cancer (BC) is the most frequently diagnosed cancer and the leading cause of cancer-associated deaths in women. Recent studies have indicated that microRNA (miRNA) regulation in genomic instability (GI) is associated with disease risk and clinical outcome. Herein, we aimed to identify the GI-derived miRNA signature in extracellular vesicles (EVs) as a minimally invasive biomarker for early diagnosis and prognostic risk stratification. Experimental design Integrative analysis of miRNA expression and somatic mutation profiles was performed to identify GI-associated miRNAs. Then, we constructed a discovery and validation study with multicenter prospective cohorts. The GI-derived miRNA signature (miGISig) was developed in the TCGA discovery cohort (n = 261), and was subsequently independently validated in internal TCGA validation (n = 261) and GSE22220 (n = 210) cohorts for prognosis prediction, and in GSE73002 (n = 3966), GSE41922 (n = 54), and in-house clinical exosome (n = 30) cohorts for diagnostic performance. Results We identified a GI-derived three miRNA signature (MIR421, MIR128-1 and MIR128-2) in the serum extracellular vesicles of BC patients, which was significantly associated with poor prognosis in all the cohorts tested and remained as an independent prognostic factor using multivariate analyses. When integrated with the clinical characteristics, the composite miRNA-clinical prognostic indicator showed improved prognostic performance. The miGISig also showed high accuracy in differentiating BC from healthy controls with the area under the receiver operating characteristics curve (ROC) with 0.915, 0.794 and 0.772 in GSE73002, GSE41922 and TCGA cohorts, respectively. Furthermore, circulating EVs from BC patients in the in-house cohort harbored elevated levels of miGISig, with effective diagnostic accuracy. Conclusions We report a novel GI-derived three miRNA signature in EVs, as an excellent minimally invasive biomarker for the early diagnosis and unfavorable prognosis in BC.

scholarly journals Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula

Nanoscale ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 218-232
Author(s):  
Arthur Berger ◽  
Irami Araújo-Filho ◽  
Max Piffoux ◽  
Alba Nicolás-Boluda ◽  
Alice Grangier ◽  
...  
Keyword(s):  
Stem Cell ◽  
Minimally Invasive ◽  
Therapeutic Effect ◽  
Extracellular Vesicles ◽  
Residence Time ◽  
Rat Model ◽  
Local Administration ◽  
Thermoresponsive Hydrogel ◽  
Healing Effect

Local minimally-invasive EV delivery on a thermo-actuated PF-127 gel enhanced EV residence time in colo-cutaneous fistulas promoting a therapeutic effect.

scholarly journals Extracellular Vesicles as Biomarkers in Cancer Immunotherapy

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2825
Author(s):  
Matthen Mathew ◽  
Mariam Zade ◽  
Nadia Mezghani ◽  
Romil Patel ◽  
Yu Wang ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Cancer Immunotherapy ◽  
Extracellular Vesicles ◽  
Checkpoint Inhibitors ◽  
Tissue Sample ◽  
Unmet Need ◽  
Cell Types ◽  
Molecular Signature ◽  
Histologic Diagnosis ◽  
Targeted Analysis

Extracellular vesicles (EVs), including exosomes and microvesicles, are membrane-bound vesicles secreted by most cell types during both physiologic conditions as well in response to cellular stress. EVs play an important role in intercellular communication and are emerging as key players in tumor immunology. Tumor-derived EVs (TDEs) harbor a diverse array of tumor neoantigens and contain unique molecular signature that is reflective of tumor’s underlying genetic complexity. As such they offer a glimpse into the immune tumor microenvironment (TME) and have the potential to be a novel, minimally invasive biomarker for cancer immunotherapy. Immune checkpoint inhibitors (ICI), such as anti- programmed death-1(PD-1) and its ligand (PD-L1) antibodies, have revolutionized the treatment of a wide variety of solid tumors including head and neck squamous cell carcinoma, urothelial carcinoma, melanoma, non-small cell lung cancer, and others. Typically, an invasive tissue biopsy is required both for histologic diagnosis and next-generation sequencing efforts; the latter have become more widespread in daily clinical practice. There is an unmet need for noninvasive or minimally invasive (e.g., plasma-based) biomarkers both for diagnosis and treatment monitoring. Targeted analysis of EVs in biospecimens, such as plasma and saliva could serve this purpose by potentially obviating the need for tissue sample. In this review, we describe the current challenges of biomarkers in cancer immunotherapy as well as the mechanistic role of TDEs in modulating antitumor immune response.

scholarly journals Tumor-derived extracellular vesicles: Potential tool for cancer prognosis, diagnosis, and therapy

2022 ◽  
Author(s):  
Tayyaba Saleem ◽  
Aleena Sumrin ◽  
Muhammad Bilal ◽  
Hamid Bashir ◽  
Muhammad Babar Khawar
Keyword(s):  
Extracellular Vesicles ◽  
Cancer Prognosis ◽  
Diagnosis And Therapy ◽  
Potential Tool

scholarly journals Affinity Captured Urinary Extracellular Vesicles Provide mRNA and miRNA Biomarkers for Improved Accuracy of Prostate Cancer Detection: A Pilot Study

2020 ◽  
Vol 21 (21) ◽  
pp. 8330
Author(s):  
Michelle Davey ◽  
Sami Benzina ◽  
Marc Savoie ◽  
Guy Breault ◽  
Anirban Ghosh ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Minimally Invasive ◽  
Extracellular Vesicles ◽  
Prostate Cancer Screening ◽  
Quantitative Polymerase Chain Reaction ◽  
Specific Antigen ◽  
Area Under The Curve ◽  
Test Accuracy ◽  
Expression Ratio ◽  
Tissue Biopsy

Serum prostate-specific antigen (sPSA) testing has helped to increase early detection of and decrease mortality from prostate cancer. However, since sPSA lacks specificity, an invasive prostate tissue biopsy is required to confirm cancer diagnosis. Using urinary extracellular vesicles (EVs) as a minimally invasive biomarker source, our goal was to develop a biomarker panel able to distinguish prostate cancer from benign conditions with high accuracy. We enrolled 56 patients in our study, 28 negative and 28 positive for cancer based on tissue biopsy results. Using our Vn96 peptide affinity method, we isolated EVs from post-digital rectal exam urines and used quantitative polymerase chain reaction to measure several mRNA and miRNA targets. We identified a panel of seven mRNA biomarkers whose expression ratio discriminated non-cancer from cancer with an area under the curve (AUC) of 0.825, sensitivity of 75% and specificity of 84%. We also identified two miRNAs whose combined score yielded an AUC of 0.744. A model pairing the seven mRNA and two miRNA panels yielded an AUC of 0.843, sensitivity of 79% and specificity of 89%. Addition of EV-derived PCA3 levels and clinical characteristics to the biomarker model further improved test accuracy. An AUC of 0.955, sensitivity of 86% and specificity of 93% were obtained. Hence, Vn96-isolated urinary EVs are a clinically applicable and minimally invasive source of mRNA and miRNA biomarkers with potential to improve on the accuracy of prostate cancer screening and diagnosis.

scholarly journals Extracellular Vesicles in Liquid Biopsies: Potential for Disease Diagnosis

2021 ◽  
Vol 2021 ◽  
pp. 1-17
Author(s):  
Jialing Liu ◽  
Ye Chen ◽  
Fang Pei ◽  
Chongmai Zeng ◽  
Yang Yao ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Minimally Invasive ◽  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Enzymatic Degradation ◽  
Disease Diagnosis ◽  
Sample Collection ◽  
Liquid Biopsies ◽  
Promising Tool ◽  
Extracellular Environment

Liquid biopsy is conducted through minimally invasive or noninvasive procedures, and the resulting material can be subjected to genomic, proteomic, and lipidomic analyses for early diagnosis of cancers and other diseases. Extracellular vesicles (EVs), one kind of promising tool for liquid biopsy, are nanosized bilayer particles that are secreted by all kinds of cells and that carry cargoes such as lipids, proteins, and nucleic acids, protecting them from enzymatic degradation in the extracellular environment. In this review, we provide a comprehensive introduction to the properties and applications of EVs, including their biogenesis, contents, sample collection, isolation, and applications in diagnostics based on liquid biopsy.

scholarly journals Translational Potential of RNA Derived From Extracellular Vesicles in Multiple Myeloma

2021 ◽  
Vol 11 ◽  
Author(s):  
Antonia Reale ◽  
Tiffany Khong ◽  
Sridurga Mithraprabhu ◽  
Andrew Spencer
Keyword(s):  
Multiple Myeloma ◽  
Drug Resistance ◽  
Minimally Invasive ◽  
Extracellular Vesicles ◽  
Cross Talk ◽  
Stromal Cells ◽  
Biological Features ◽  
Isolation And Characterization

The cross-talk between tumour cells and stromal cells is a hallmark of multiple myeloma (MM), a blood cancer that still remains incurable despite increased knowledge of its biology and advances in its treatment. Extracellular vesicles (EVs) derived from both tumour and stromal cells have been shown to play an important role in mediating this cross-talk ultimately favouring MM progression and drug resistance. Furthermore, EVs and their content including RNA (EV-RNA) have been successfully isolated from blood and are being explored as liquid biomarkers in MM with the potential to improve diagnosis and monitoring modalities with a minimally-invasive and repeatable analysis, i.e. liquid biopsy. In this review, we describe both the role of EV-RNA in defining the biological features of MM and their potential translational relevance as liquid biomarkers, therapeutic targets and delivery systems. We also discuss the limitations and technical challenges related to the isolation and characterization of EVs and provide a perspective on the future of MM-derived EV-RNA in translational research.

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