Atividade Antitumoral de Extratos obtidos do Epicarpo de Plinia cauliflora (Mart.) Kausel sobre Tumor Sólido de Ehrlich/ Antitumoral Activity of Extracts Obtained from Epicarp of Plinia cauliflora (Mart.) Kausel on Solid Ehrlich Tumor

Abstract Background Cancer is a multifactorial disease caused by uncontrolled proliferation of cells. About 50–80% of cancer patients develop cachexia, a complex metabolic syndrome associated with an increase of mortality and morbidity. However, there are no effective therapies in medical clinic for cancer cachexia. Vochysia tucanorum Mart. is a common three of the Brazilian “Cerrado”. The butanolic fraction of V. tucanorum (Fr-BuVt), very rich in triterpenes with various biological activities, might be interesting in being tested in cancer cachexia syndrome. Hence, the present study was undertaken to investigate the antitumoral activity of Fr-BuVt and its potential against cachexia development. Methods Ehrlich tumor was used as model of cancer cachexia. Ascitic Ehrlich tumor cells were collected, processed and inoculated subcutaneously in saline solution (1 × 107/100 μl; ≥95% viability) for the obtention of solid Ehrlich carcinoma. After inoculation, solid Ehrlich carcinoma-bearing mice were treated by 14 consecutive days by gavage with Fr-BuVt (200 mg/kg). Body weight and tumor volume were measure during the treatment period. Tumors were removed, weighed and properly processed to measure the content and phosphorylation levels of key-proteins involved to apoptotic and proliferation process by Western Blot. Muscles and adipose tissues were removed for weighed. Serum was collected to cytokines levels and energetic blood markers measurements. Results The treatment with the Fr-BuVt (200 mg/kg, 14 days) decreased the solid Ehrlich tumor volume and weight besides increased the expression of the pro-apoptotic proteins caspase-3 and BAX, but also decreased the expression of the proteins involved in proliferation NFκB, mTOR and ERK. In addition, our data shows that the administration of Fr-BuVt was able to prevent the installation of cancer cachexia in Ehrlich carcinoma-bearing mice, since prevented the loss of body weight, as well as the loss of muscle and adipose tissue. Moreover, an improvement in some blood parameters such as decrease in cytokines TNF-α and IL-6 levels is observed. Conclusions The study revealed that Fr-BuVt has antitumoral activity and prevent installation of cancer cachexia in Ehrlich model. Therefore, Fr-BuVt may represent an alternative treatment for cancer cachexia.

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Development and Characterization Colchicine-Loaded PEGylated Gelatin Nanoparticles for Targeted Delivery to Tumor

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2013.6.2.8 ◽

2013 ◽

Vol 6 (2) ◽

pp. 2058-2063

Author(s):

G D Chandrethiya ◽

P K Shelat ◽

M N Zaveri

Keyword(s):

Drug Delivery ◽

Targeted Delivery ◽

High Performance ◽

Entrapment Efficiency ◽

Stability Study ◽

Spherical Particles ◽

Precipitation Method ◽

Antitumoral Activity ◽

Gelatin Nanoparticles

PEGylated gelatin nanoparticles loaded with colchicine were prepared by ethanol precipitation method. Poly-(ethylene glycol)-5000-monomethylether (MPEG 5000), a hydrophilic polymer, was used to pegylate gelatin. Gluteraldehyde was used as cross-linking agent. To obtain a high quality product, major formulation parameters were optimized. Spherical particles with mean particles of 193 nm were measured by a Malvern particle size analyzer. Entrapment efficiency was found to be 71.7 ± 1.4% and determined with reverse phase high performance liquid charomatography (RP-HPLC). The in vitro drug release study was performed by dialysis bag method for a period of 168 hours. Lyophilizaton study showed sucrose at lower concentrations proved the best cryoprotectant for this formulation. Stability study revealed that lyophilized nanoparticles were equally effective (p < 0.05) after one year of storage at 2-8°C with ambient humidity. In vitro antitumoral activity was accessed using the MCF-7 cell line by MTT assay. The IC50 value was found to be 0.034 μg/ml for the prepared formulation. The results indicate that PEGylated gelatin nanoparticles could be utilized as a potential drug delivery for targeted drug delivery of tumors.

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Molecular Docking Studies of the Antitumoral Activity and Characterization of New Chalcone

Current Topics in Medicinal Chemistry ◽

10.2174/1568026615666150427125033 ◽

2015 ◽

Vol 15 (17) ◽

pp. 1743-1749 ◽

Cited By ~ 4

Author(s):

Aurelio San-Martin ◽

Viviana Donoso ◽

Sergio Leiva ◽

Mitchell Bacho ◽

Solange Nunez ◽

...

Keyword(s):

Molecular Docking ◽

Docking Studies ◽

Antitumoral Activity ◽

Molecular Docking Studies

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The Antiangiogenic and Antitumoral Activity of Titanocene Y* In Vivo

Letters in Drug Design & Discovery ◽

10.2174/157018011794839367 ◽

2011 ◽

Vol 8 (4) ◽

pp. 302-307 ◽

Cited By ~ 10

Author(s):

Iduna Fichtner ◽

Diana Behrens ◽

James Claffey ◽

Anthony Deally ◽

Brendan Gleeson ◽

...

Keyword(s):

Antitumoral Activity

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A Review on the Biological Activity of Camellia Species

Molecules ◽

10.3390/molecules26082178 ◽

2021 ◽

Vol 26 (8) ◽

pp. 2178

Author(s):

Ana Margarida Teixeira ◽

Clara Sousa

Keyword(s):

Biological Activity ◽

Medicinal Plants ◽

Green Tea ◽

Antioxidant Properties ◽

Antitumoral Activity ◽

Camellia Oleifera ◽

Camellia Japonica ◽

Detailed Review ◽

Additional Information ◽

Camellia Species

Medicinal plants have been used since antiquity to cure illnesses and injuries. In the last few decades, natural compounds extracted from plants have garnered the attention of scientists and the Camellia species are no exception. Several species and cultivars are widespread in Asia, namely in China, Japan, Vietnam and India, being also identified in western countries like Portugal. Tea and oil are the most valuable and appreciated Camellia subproducts extracted from Camellia sinensis and Camellia oleifera, respectively. The economic impact of these species has boosted the search for additional information about the Camellia genus. Many studies can be found in the literature reporting the health benefits of several Camellia species, namely C. sinensis, C. oleifera and Camellia japonica. These species have been highlighted as possessing antimicrobial (antibacterial, antifungal, antiviral) and antitumoral activity and as being a huge source of polyphenols such as the catechins. Particularly, epicatechin (EC), epigallocatechin (EGC), epicatechin-3-gallate (ECG), and specially epigallocatechin-3-gallate (EGCG), the major polyphenols of green tea. This paper presents a detailed review of Camellia species’ antioxidant properties and biological activity.

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On‐Chip Biogenesis of Circulating NK Cell‐Derived Exosomes in Non‐Small Cell Lung Cancer Exhibits Antitumoral Activity

Advanced Science ◽

10.1002/advs.202003747 ◽

2021 ◽

pp. 2003747

Author(s):

Yoon‐Tae Kang ◽

Zeqi Niu ◽

Thomas Hadlock ◽

Emma Purcell ◽

Ting‐Wen Lo ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Nk Cell ◽

Small Cell ◽

Antitumoral Activity ◽

Small Cell Lung ◽

On Chip

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Redefining interferon-producing killer dendritic cells as a novel intermediate in NK-cell differentiation

Blood ◽

10.1182/blood-2011-11-395954 ◽

2012 ◽

Vol 119 (19) ◽

pp. 4349-4357 ◽

Cited By ~ 18

Author(s):

Fanny Guimont-Desrochers ◽

Geneviève Boucher ◽

Zhongjun Dong ◽

Martine Dupuis ◽

André Veillette ◽

...

Keyword(s):

Dendritic Cells ◽

Nk Cells ◽

Adoptive Transfer ◽

Nk Cell ◽

Cell Lineage ◽

Current View ◽

Antitumoral Activity ◽

Nk Cell Differentiation ◽

A Cell

Abstract The cell lineage origin of IFN-producing killer dendritic cells (IKDCs), which exhibit prominent antitumoral activity, has been subject to debate. Although IKDCs were first described as a cell type exhibiting both plasmacytoid DC and natural killer (NK) cell properties, the current view reflects that IKDCs merely represent activated NK cells expressing B220, which were thus renamed B220+ NK cells. Herein, we further investigate the lineage relation of B220+ NK cells with regard to other NK-cell subsets. We surprisingly find that, after adoptive transfer, B220− NK cells did not acquire B220 expression, even in the presence of potent activating stimuli. These findings strongly argue against the concept that B220+ NK cells are activated NK cells. Moreover, we unequivocally show that B220+ NK cells are highly proliferative and differentiate into mature NK cells after in vivo adoptive transfer. Additional phenotypic, functional, and transcriptional characterizations further define B220+ NK cells as immediate precursors to mature NK cells. The characterization of these novel attributes to B220+ NK cells will guide the identification of their ortholog in humans, contributing to the design of potent cancer immunotherapies.

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